Correction to your video: Succintly, phosphorylated retina blastoma renders dissociation from E2F. Subsequently, E2F can translocate to the nucleus to confer increased expression of E2F target genes, including E2F (positive feedback loop) and cyclin E to propogate entry into S-phase. CDK = Cyclin-Dependent-Kinase. Per definition, kinases phosphorylate (add phosphor groups to) targets. Thus, CDK will phosphorylate retina blastoma to confer its dissociation from E2F.
@kalyanRanjit-u4z3 ай бұрын
Yes the CDK2/Cyclin E phosphorylates PrB, which can release the factors that allow G1 to S.
@AnchaliJunn4 ай бұрын
Very nice explanation 😺
@reezis16194 жыл бұрын
doesn't the retinoblastic protein release E2F after phosphorylation?
@jonathanlehmann20594 жыл бұрын
I thought so too. From ‘Signal Transduction, Principles, Pathways, and Processes’, page 141: “Phosphorylation of the pRB family proteins by CDKs during G1 phase causes pRB to dissociate from E2Fs, allowing transcription of target genes that stimulate progression into S phase.”
@reezis16194 жыл бұрын
@@jonathanlehmann2059 Yeah. That is what it says in my textbook as well
@jonathanlehmann20594 жыл бұрын
milk is cheap protein Good to know, thanks. Mine is a 2013 book, so it’s possible that the field has changed since then, maybe.
@sudiptasarma Жыл бұрын
nicely explained.
@alhaeri13 жыл бұрын
in my opinion it should be more like high degredation of p53 in the case of low stress and low degredation of p53 in the case of high stress
@dontmissthelittlethings2 жыл бұрын
Thanks for the information.
@killianpapail9324 Жыл бұрын
Isn't the cdk4/6-cyclinD complex which phosphorylates Rb to release E2F ?
@fatahiyakashif81993 жыл бұрын
pRb is inactivated when phosphorylated and then it releases E2F which enters the nucleus and acts as a transcription factor for proteins that help in G1 to S phase transition.
@seanclark64384 жыл бұрын
Just double checking but is P21 negative my regulated by MDM2
@fayazferoz79784 жыл бұрын
it is p53 that is degraded by the action of MDM2
@seanclark64384 жыл бұрын
Fayaz Feroz Thanks couldn’t quite remember, when revising this I looked up all the gene loci, thanks to the wink wink to revise p53 and non of it came up on the exam