The Principle of Agarose Gel Electrophoresis, a full explanatory video

  Рет қаралды 163,667

Biomedical and Biological Sciences

Biomedical and Biological Sciences

Күн бұрын

Пікірлер: 104
@romelsoyza4160
@romelsoyza4160 6 жыл бұрын
A complete and very easy to understand explanation (like the videos for Flow Cytometry and FACS) on the principles behind the technique.
@AyazSamo
@AyazSamo 5 жыл бұрын
She is great a great teacher! Respects from Sindh!
@سمرعبدالناصر-و2م
@سمرعبدالناصر-و2م Жыл бұрын
بجد احسن حد شرح الموضوع دة بالتوفيق❤
@srigayathri5980
@srigayathri5980 3 жыл бұрын
Thank you so muck mam.Because of u i can able to understand the concept now mam.
@Animelover7948
@Animelover7948 3 жыл бұрын
Very very well explained...very helpful....people out there can try watching this vid
@mohammedholba2498
@mohammedholba2498 6 жыл бұрын
One of the best youtube chanel l ever seen. :)
@VarshiiiPrem
@VarshiiiPrem 5 жыл бұрын
A great teacher..... Thanks a lot... Tomorrow is my exam... Very useful
@sanskrutiraj8051
@sanskrutiraj8051 Жыл бұрын
This video is very helpful for me. Thank you u explained in easy way 🙂
@stephaniehurtado4096
@stephaniehurtado4096 3 жыл бұрын
Late to the upload but I’ve watch almost all your video and they all been so helpful:) ofc ill like and subscribe!
@qamarhennawi9137
@qamarhennawi9137 3 жыл бұрын
You are the best, no one like you
@joan-b-empire576
@joan-b-empire576 2 жыл бұрын
Love from Cameroon 🇨🇲 thank you
@statusboys4036
@statusboys4036 2 жыл бұрын
God give me an opportunity to spend 15 mins of my life time in a precious way...
@walrus4248
@walrus4248 4 жыл бұрын
Will see if you already did capillary electrophoresis, this was such a fine explanation thanks.
@abdulmunafisalisuumar7016
@abdulmunafisalisuumar7016 Жыл бұрын
thank you so much maam, for your wonderful explanation of Principle of Agarose gel electrophoresis.
@yamaatomayi4315
@yamaatomayi4315 6 жыл бұрын
mem i like your explanation, i love to hear u r voice again and again u r a great talent .. and i really love..
@briang1310
@briang1310 5 жыл бұрын
why do indian people always says mem or ma'am
@gabecalderon2043
@gabecalderon2043 3 жыл бұрын
@Sandeep Kumar learn respect then
@mohammedal-hammadi5085
@mohammedal-hammadi5085 5 жыл бұрын
Wow, this video is so clear and useful, besides you are awesome, thank you so much
@sahewa100
@sahewa100 7 жыл бұрын
Very well explained.... thanx alot for making such video. This helped a lot in my thesis research. Please continue to teach us :))
@biomedicalandbiologicalsci4989
@biomedicalandbiologicalsci4989 7 жыл бұрын
Thank you for your support :)
@ladushky1
@ladushky1 4 жыл бұрын
Thank you! we have never add the colour right into the gel tho.. but we do add it to the samples we are running on the gel.
@gilyasungi4572
@gilyasungi4572 5 жыл бұрын
Well explained! Good explanations linked with real life applications.
@Gbemi78
@Gbemi78 5 жыл бұрын
your explanation is second to none.
@lincolnkarim1
@lincolnkarim1 3 жыл бұрын
This is not my field at all so please excuse my ignorance; Since DNA is a negative molecule (3:48), does that mean it is an ion?
@wonderakpese1901
@wonderakpese1901 2 жыл бұрын
Concept well explained. Thank you
@walrus4248
@walrus4248 4 жыл бұрын
Fantastic, I learned a lot and I didn't know anything about the subject.
@RohitPant04
@RohitPant04 3 жыл бұрын
Concise & informative! Good job. 🙏
@trona2612
@trona2612 6 жыл бұрын
Hi Ma'am, thank you very much for such a good,short & crisp but very much informative video on agarose gel electrophoresis.... I've a question on the differential mobility profile of nicked & linear DNA molecules of same size; why do linear DNA molecule migrates ahead of nicked one? I'll will be grateful to hear from you. Thank you!
@muhammadjaber2272
@muhammadjaber2272 6 жыл бұрын
Your voice like best for presenting and I have get many from your channel
@worldaviation4k
@worldaviation4k 4 жыл бұрын
so this isn't used to be able to look at the dna letters? Thanks I've had trouble trying to find the videos where you take the dna and look at the actual proteins. maybe i will find it soon :)
@krisantinimarkam3396
@krisantinimarkam3396 2 жыл бұрын
Very well explained and easy to understand. Thank you
@ridzwanfauzi975
@ridzwanfauzi975 4 жыл бұрын
great video!!really helped me in my test..thank you so much
@poetrylover5561
@poetrylover5561 3 жыл бұрын
You've nicely explained the topic. Many thanks for sharing ♥️
@Stonkonly
@Stonkonly 6 жыл бұрын
very informative video, explained in detail for the reason of each step.
@soheilaazam799
@soheilaazam799 4 жыл бұрын
You are the best molec teacher, who I have ever seen!!! Well done 👍😊 and thanks for the Videos. What is your name by the way???
@dstan16224
@dstan16224 5 жыл бұрын
Thank you very much ma'am.It really helped a lot.
@fasilutubechannel6697
@fasilutubechannel6697 3 жыл бұрын
thanks i hope another video release in this related video
@ciao_abhi
@ciao_abhi 6 жыл бұрын
This helped me so much for my mcat test
@maggiejameel6725
@maggiejameel6725 6 жыл бұрын
Very well explained, you are an awesome teacher. I SUBSCRIBED
@Aendrila231
@Aendrila231 3 жыл бұрын
Thank you Ma'am.. It's a great video! ❤
@mohaninos64
@mohaninos64 6 жыл бұрын
Such a good and simply way of explanation thank you
@sukanyahembrom6213
@sukanyahembrom6213 7 жыл бұрын
very well explained.... thanx alot for making such video
@tinyikonkuna1479
@tinyikonkuna1479 2 жыл бұрын
😊😊😊😊Love it here plz make more videos on biology concepts especially 4 varsity students
@nextagro
@nextagro 4 жыл бұрын
Not sure if the comments are still answered, but... Why do we need a PLATINUM wire electrode in the electrophoresis setup? What exactly will happen if one replaces that electrode with an ordinary Copper wire?
@rahmanebrahimzadegan7198
@rahmanebrahimzadegan7198 3 жыл бұрын
You teach excellent. Thank you.
@Min-gh1jr
@Min-gh1jr 6 жыл бұрын
Hi this video is great! I was wondering if the thickness of the band would suggest any properties about the nucleic acid strand, i.e there are more nucleic acid fragments of that size?
@jaswantnegi4879
@jaswantnegi4879 5 жыл бұрын
Please upload some videos about difference between RNA and DNA isolation and separation techniques. Do tell about Difference in their agarose gel concentration and gel separating chamber, where we use horizontal or vertical gel chambers...
@setarehsohail5422
@setarehsohail5422 2 жыл бұрын
It was a very nice lecture!
@bahahos7426
@bahahos7426 5 жыл бұрын
Thank u very much i love all your videos
@iliyamohd8154
@iliyamohd8154 3 жыл бұрын
Very good presentation
@yennguyen-tp7ce
@yennguyen-tp7ce 7 жыл бұрын
everything is just so clear, thanks
@ikhinerawlings124
@ikhinerawlings124 2 жыл бұрын
Well explained. Thanks. Can talk on chromatography in details
@pritichristian2673
@pritichristian2673 3 жыл бұрын
Thanks. Very clear explanation
@stevemorton3078
@stevemorton3078 4 жыл бұрын
Are PCR and then AGE being done in an automated fashion on a single sample in a miniature electrochemical device, such as one that can only be used once ?
@jyothinandakumar6190
@jyothinandakumar6190 Жыл бұрын
Very well explained
@tanimaferdous50
@tanimaferdous50 7 жыл бұрын
Very very good explanation
@laidewasiu2310
@laidewasiu2310 5 ай бұрын
How can one read a plasmid using three different enzymes to cut
@shailendrayadav5799
@shailendrayadav5799 5 жыл бұрын
You are great teacher mam
@krishnanandh7999
@krishnanandh7999 3 жыл бұрын
Supr class. Thank you🤩🤩
@Katherine-mf9wz
@Katherine-mf9wz 8 ай бұрын
Nice job!
@felixjohnpaulbarqueros6675
@felixjohnpaulbarqueros6675 5 жыл бұрын
Hi how can I determine the effectiveness if i will alternative coloring dye for the loading buffer
@robinkhan6419
@robinkhan6419 3 жыл бұрын
Well presentation
@saraabdulaziz3764
@saraabdulaziz3764 3 жыл бұрын
Very informative🥰
@girlschannel11
@girlschannel11 6 жыл бұрын
Really good explanation !!!!
@halafr5861
@halafr5861 6 жыл бұрын
You are the best thank you from my heart 💜
@samanthabautista1655
@samanthabautista1655 6 жыл бұрын
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
@bharathkumart6097
@bharathkumart6097 5 жыл бұрын
Thank you very much for this video ma'am
@dikshashreedevi4568
@dikshashreedevi4568 5 жыл бұрын
please make some more videos .good explanation
@jthomas0007
@jthomas0007 3 жыл бұрын
Hi ma'am, could you please talk about PCR and it's various types?
@emanwanli7740
@emanwanli7740 4 жыл бұрын
I have a question .. when migrating the genomic DNA .. shouldn't multiple bandits appear, each expressing a single chromosome content .. since chromosomes carry DNA of very different sizes, especially if we are talking about humans, for example
@youcare6415
@youcare6415 7 жыл бұрын
Very informative. I guess it is SYBR Green not cyber green ;)
@anirbanhait5998
@anirbanhait5998 2 жыл бұрын
Ma'am have pronounced rightly.
@SLNmin
@SLNmin 2 жыл бұрын
It is SYBR, pronounced as cyber but correct spelling is SYBR.
@claudiaaurie8161
@claudiaaurie8161 7 жыл бұрын
Hi! I just found you and I have been watching your videos. They are amazing! I was wondering (this may be a silly question) but how do you know which restriction enzymes to use for example for paternity testing? does it matter which to use as long as all DNA samples are treated the same? thank you! look forward to watching all your videos--Claudia
@biomedicalandbiologicalsci4989
@biomedicalandbiologicalsci4989 7 жыл бұрын
Hei .. thank you for your comment ... as you said, you should treat all the samples with the same restriction enzyme .. and experimentation will show us which is the best restriction enzyme to use :)
@sidratulmuntaha1687
@sidratulmuntaha1687 6 жыл бұрын
@@biomedicalandbiologicalsci4989 Can we search it out from the literature which endonuclease to be used?
@stevanstankovic8021
@stevanstankovic8021 4 жыл бұрын
Great video, thanks! I have a question concerning visualisation: If after the gel we have the blue bands with the loadind dye, why do we need to add Ethidium bromide to make it visible under UV? Isn't the information we have from the blue bands sufficient? Or is it maybe, that the blue bands we see after we ran the gel is maybe almost only the dye itself, as it leaves the DNA (or RNA) and just sinks faster to the + side, so that our actual DNA (or RNA) is located a bit above the dye - and that is why we need to check it under UV, as it would be "invisibly" located above the dye? Really can't find any answer to this! Thanks again! (:
@ladushky1
@ladushky1 4 жыл бұрын
We either add GelRed right into the gel or we use ethidium bromide later for visualization; both are carcinogens but we would not use both at the same time.
@geeswags6172
@geeswags6172 3 жыл бұрын
Informative
@nagendrag7562
@nagendrag7562 4 жыл бұрын
explained very nicely
@insanhabib4497
@insanhabib4497 4 жыл бұрын
Thanks a lot, ma'am.
@kuldeepdinkar4918
@kuldeepdinkar4918 6 жыл бұрын
What is the use of nylon fiber?
@urmilahussainpiya6735
@urmilahussainpiya6735 6 жыл бұрын
please make a vedio why Taq DNA polymerase is thermostable
@Milahh.
@Milahh. 7 жыл бұрын
Thx, very useful well done
@biomedicalandbiologicalsci4989
@biomedicalandbiologicalsci4989 7 жыл бұрын
Thank you for your comment ...
@Art-cq1zy
@Art-cq1zy 2 жыл бұрын
Im confused. Why does the solution not evaporate in the microwave?
@drgaikwadsir7270
@drgaikwadsir7270 5 жыл бұрын
Make some videos related with r dDNA technology
@devyaniitware2428
@devyaniitware2428 6 жыл бұрын
What are amplicons?
@jiaflair
@jiaflair 3 жыл бұрын
THANKYOU 💞
@Az-xr5yx
@Az-xr5yx 5 жыл бұрын
Thanx thats was helpfull
@thnxm8
@thnxm8 3 жыл бұрын
Thanks a lot
@ranaaamiraamir3437
@ranaaamiraamir3437 6 жыл бұрын
Brilliant
@abeerm9786
@abeerm9786 5 жыл бұрын
Thank you
@MaryGraceBayot
@MaryGraceBayot Жыл бұрын
Thanks
@redeemerbadagbor1673
@redeemerbadagbor1673 9 ай бұрын
you do all :salut
@drgaikwadsir7270
@drgaikwadsir7270 5 жыл бұрын
Nice mam
@Killer-v4j
@Killer-v4j 10 ай бұрын
❤❤❤
@wycliffenyandika9017
@wycliffenyandika9017 Жыл бұрын
like it
@sukantamandal8106
@sukantamandal8106 5 жыл бұрын
Replication
@jsvclubdeciencia6283
@jsvclubdeciencia6283 3 жыл бұрын
In silico: kzbin.info/www/bejne/eJy3g4yDn96UqqM
@tıbhendese
@tıbhendese 4 жыл бұрын
I don't think anything in nature and universe is junk or useless
@ladushky1
@ladushky1 4 жыл бұрын
Sorry, but there is no such thing as DNA junk sequence anymore.
@samanthabautista1655
@samanthabautista1655 6 жыл бұрын
Hi im just wondering which buffer to use when your stain is acidic? Because according to a journal ive read that stain that we are about to use when expsed to basic enviroment it can affect the staining capability in a bad way? So which buffers to use??? Anyone can answer? It would help me a lot for our research please
@joyeke6340
@joyeke6340 3 жыл бұрын
Thank you
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