Tissue Culture Lab /tissue culture lab complete tutorial

[@raj4104]

Informative video.Thank you.

[@johndavid4825]

Very nice video. Thank you for sharing. I stumbled onto this video and noticed that you are using what appears to be saran-wrap for the tissue cultures (around 2:25 minutes into the video), closed with a rubber band. I've never seen this done before. Do you have any details on the technique? How well does it work for keeping the tissue cultures sterile?

[@Taslima-Abshaar]

Awesome

[@botanicalinformation8260]

thank you

[@wickittiewhaat2495]

Wow such tremendous tedious work! Thank you for sharing 🎉

[@aminasaeed4386]

Thank u

[@botanicalinformation8260]

thank u

[@mahorajpoot2600]

Gud work Very informative Just keep it up dude 🎗️🌻

[@botanicalinformation8260]

Thanks a ton

[@astilekuriakose2422]

What media did you use for growing plantlets?

[@botanicalinformation8260]

MS media

[@muhammadshahid4820]

MS media having 4mg per liter BAP

[@bascik]

Hey, what type of autoclave is it?? thank you for sharing!! Really appreciate the tour

[@gruit5876]

Reinheldt GMBH, the website is german laboratory equipment manufacturer. It says it on the interface of the top of the machine in the video. One year later😆

[@gruit5876]

Well I think the hardest and maybe most expensive part is the incubation room. I understand having separate rooms for each step minimizes contamination when you have more than one person working at the sametime, but for me I feel like a 4x8 tent with positive pressure air would be good for the mixing of media and the culture room as long as the air is filtered inside I would just run the laminar filter for 30 minutes before starting. If your culture is in vitro and doesn't have a open culture lid, I'm assuming the incubation room doesn't have to have controlled humidity?

[@LakshmiLakshmi-bb3kb]

Sir lab lo work chesthe anny health issues pls cheppandi

[@dinocageman2311]

Great video . What kind of lights are used ?

[@greenhut1642]

FROM WHERE CAN THISAUTO CLAVE PURCHASED

[@comedyworld4005]

Good

[@botanicalinformation8260]

Thanks

[@paulaz.flaquer9570]

Can you use a vertical laminar flow hood on plant tissue culture?

[@juis_arttheatre2998]

Where is this setup?

[@botanicalinformation8260]

Faisalabad Punjab Pakistan

[@muhammadshahid4820]

Vegetable Research Institute AARI Faisalabad Pakistan

[@ChampionsPath11]

That girl should had used gloves 😂

[@sunshinevalley0]

Gloves are probably too expensive ❤

[@arcticwolf9876]

nope using the gloves with an open flame for sterilization can cause serious burns if you even touch the flame, the whole glove catches fire and you have melted plastic on your hands@@sunshinevalley0

[@hemshankarsahu192]

"Where are perches for Auxins like IBA, NAA and Cytokinins like Kinetin purchased for research purposes, specifically for best root induction and root culture?"

[@kavyakotian6583]

Which explant you used here?

[@aminasaeed4386]

Bud

[@botanicalinformation8260]

bud

[@muhammadshahid4820]

Ginger buds

[@hafizishtiaqahmad6257]

Where is this lab ?? Which university? BTW awesome 👍👍

[@botanicalinformation8260]

AARI punjab pakistan

[@dootpooter4740]

@@botanicalinformation8260 tell all my pakistani homies I said whats upppppp

[@muhammadshahid4820]

Vegetable Research Institute AARI. Faisalabad Pakistan

[@muhammadshahid4820]

This is Ginger tissue culture lab at Vegetable Research Institute AARI, Faisalabad Pakistan Dr. Tasdiq Hussain