Plant Tissue Culture 101 | With Demonstration! | The 'Breaking Bad' of Houseplants!

[@ZipinS1]

I'm doing my PhD in plant biology, and I'm impressed and approve of the quality and explainations! You made sure not to miss anything! great video! also, our Lab is NOT sterile. Soil everywhere. the laminar flow hood is sufficient for sterile work!

[@Anne-tl9ug]

Microbologist here, same same :-). Having a sterile lab it is not necessary for us. However, the situation is different in e.g. a medical device cleanroom, in which manufacturing and filling has to be aseptic.

[@tere6024]

I'm studying plant biology too!! 😍

[@Marie-hu7xd]

Zip Lynx biology student here and I can only agree!! You explained this better and clearer than a lot of academics do

[@erin-iq8tm]

omg im 15 and its my dream to get a botany degree and hopefully PhD 🌿

[@Tommy-dt6xl]

Biology student here too, same with me can just agree💪🏽👍🏽

[@maxwinkeljohn]

As someone who works in a tissue culture research lab, I’m very impressed! I think you did a better job explaining the basics of the process than I probably could 😂 Also, as a few other people mentioned, the laminar flow hood is pretty much the only sterile space in our lab lol. But overall, loved the video! 😊

[@kirstilipphardt4713]

I also work in a research lab with tissue culture! What kind of plants are u working with? We focus on strawberries and raspberries. She makes it sound fancier than it is lol

[@ld4028]

@@kirstilipphardt4713 that’s really interesting to hear someone say. What do you think would be an easier to digest overview or resource for n00bs like me?

[@FiddleSticks800]

There is one thing she missed. Sure, a lot of plant-specific culture mediums are proprietary, but the scientific literature is filled with a lot of recipes and techniques.

[@KR-zg5uo]

You looking for staff? I cannot deal with the world anymore, need to retreat to a lab with plants lol

[@aryanprivilege9651]

It’s all easy, not like having kingdoms, not mere plants and orchids forever. In addition to human lines of forever endless additive’s incubators giants multiple, moving bottles, epic’s. You of course have multiple autoclave mechanics, build a pressure cooker, Large heavy duty, gas,…actual chemicals in more like shampoos and reagents for design, wasting, no rna dna synthesizers had in undergrad. Plasmids, viral not a Chem drug bent protein? Simplistic will likely be. It’s not to kick it novel or needed drugs especially custom w/o anaphylaxis. Add vitamins, they’ll do weed and lophora peyote like have there. Graft error, custom grow unit none heard of hybrid species and loosing ie haas..

[@juliepeterson1550]

This is so fascinating! Please do follow up videos so we can see the rest of the process as you go! I’m so interested in trying this eventually!

[@lisaeiber4450]

Great explanation! Just wanted to add a little nugget: This whole process can even be done with single plant cells! Just take a plant cell, remove cell wall so you end up with a protoplast, and put it in the right medium so a new plant can grow, simple as that huh? All this is possible because plant cells are totipotent, I just think that's really really cool! Biotechnology hey!

[@chiahuei]

we don't even need a node? that's amazing. i can try it on nicer plants without sacrificing a whole node then!

[@carlosg9934]

​@@chiahuei I believe it would only work on certain cells, which would differ from different types of plant. We would need to identify a piece of the plant which produces cells that allow "Differentiation" i.e to become root cells, node cells etc. I've only learned about this today so I'm just scratching the surface but I'm pretty sure it's not as simple as taking any old cell.

[@traumahealingandprevention]

@@carlosg9934 unless new research has evolved that I am not aware of, all plant am cells are totipotent versus most animal cells that are pluripotent. Therefore, a single plant cell has all the genetic material to grow an entire plant.

[@couchaphant]

I freaking love chemistry/hands-on science videos so I cannot tell you how much I loved this! Your graphics are so clean and your explanations so concise. Probably my fav video of yours

[@dopapier]

This is basically extremely good but spoilt by whoever put music over the top. It is so unnecessary.

[@TS-eo9uf]

Oh my god. I work in a biotech company doing large-scale cell culture. I have so much experience with keeping things sterile. I recently got really into houseplants... I think you just introduced me to my new hobby... :) I had no idea "kitchen culture" existed until just now. THANK YOU!

[@chandranthaliyil5794]

Thanks you any more links please send me

[@CrossCultural-c7f]

Interesting. Thank you. Yes, I’d like to hear a follow up.

[@Peterrdee]

Pink princess is here at the grocery store now, I laughed. 😂 I don’t have one tho, lmao

[@isabellentxawm6773]

I never knew this is how they mass produce plants. I always thought is it through seed or propagation only. So interesting. In deed it is a very tedious and long process.

[@everydaywithellamae205]

i think this is what they do in mass production in Thailand :)

[@Tismesue]

Nowhere near as long or tedious as regular propagation though.

[@pocketwatcheshit]

Dang girl your production value is looking more professional with every new video you put out! Great work!

[@sooth15]

Almost makes home propagation in a glass of water look barbaric, hahaha.

[@STRcircaFKR]

So un sterile

[@anima6035]

@@STRcircaFKR would be interested to know which plants are healthier and more resilient at the the end of it all.

[@hollydolly1994]

@@STRcircaFKR the translation 😂😂😂

[@markgarcia3485]

😂

[@dennis-fh7dt]

Yhea, but you all know your still experiment with the cup

[@MelMackey]

This style of "tutorial" is AMAZING

[@kgeorgejunior]

You invested a lot of time and resources for this video. The quality of your work is spectacular. Loved every second of it.

[@Jay-td1en]

Hands down my absolute favorite plant video. It transcends all the hauls, and care videos (which I absolutely love). But this video took plantube to a whole new level.

[@penn6693]

This was fascinating! I really appreciate videos like this - I feel like I'm actually learning when I'm watching these videos, rather than just killing time watching someone talk about 'My Top 10 Vining Plants.' (Not that videos like that are necessarily bad, we've all got time to kill these days.)

[@meeks6798]

PLEASE make sure your dish soap does not contain ammonia if you do this at home!! Mixing ammonia and bleach in any form is a big no no

[@sarahcaprez2884]

meeks can confirm 😅 it smells horrible aswell

[@everythingis2495]

A coworker at a pizza joint we worked at back in the 90s filled the mop bucket with an assortment of cleaners. Pushed buttons that dispensed surfactants, orange cleaner, WINDOW CLEANER and decided to add bleach to the cocktail. He immediately started coughing. He ran out of the kitchen. Soon the gasses started hitting other people in the restaurant. We had to evacuate the entire restaurant. It’s was definitely not good.

[@mattfletcher6815]

What if they want to get an authentic WW1 trench experience though?

[@jessicaferguson4518]

It’s been a long year, maybe some people want to

[@coagulatedsalts4711]

lets make. M U S T A R D G A S

[@erink.6420]

Oh man, as a microbiologist and plant enthusiast I would LOVE to get into this

[@louisesmith9940]

i love learning new things about plants and botanicals, thank you for all your hard work kaylee tissue culture is such an interesting topic and i'm loving every minute

[@EsspressoStresso]

Once I'm out of self isolation for the Rona I'm DEFINITELY doing this with one of my plants at my lab out of sheer curiosity. No intent to actually make it work, but it just sounds like a fun thing to do 😂😂. (I'm a research scientist and normally I work with cultures all day every day, so this is right up my alley. I can't wait to start doing science instead of paperwork when I dont have to work from home)

[@minicat3640]

Did you ever get around to it? I'd like to try this in my lab but where I work is really strict and I don't want to get in trouble. 😅

[@lindseym.8074]

@@minicat3640 Me too. I work in a biopharma lab. I'm considering asking my boss. They're going to think I'm crazy, and they'd be right. 😁

[@thomasgeorge1836]

Going back and watching these older videos from a couple years back all over again I fun. You were 100% right about the P³. In Texas we can get them at the grocery store for $20 although the varigation is hit or miss. Anyway, keep up the good work and I'd love to see more videos like this at some point once you get settled into the new house. Congrats by the way.

[@viennacreator]

can you post a video on how the kitchen tissue culture experiment turned out?

[@annevalenzuela912]

Tissue culture is very exciting! I have a protocol for pineapple, anthuriums, orchids and mutant coconut

[@plloswald]

Anne Valenzuela tell us 😛

[@aniadrabczyk8662]

Anne Valenzuela could you send these protocols?

[@izabelacont7440]

I want to se the protocol to

[@mitzivink8872]

Would you share your protocol? 🤩🤩

[@jobwong7279]

These guys who asked for protocol obviously skipped the part of the video where she explains why no one shares their protocol

[@GreenWitch1]

The intro to this video was much more pleasant than the later videos. Good video!!

[@kateoc8]

Will there be a follow up on how successful this was? I'm utterly fascinated!

[@AnaS-mn1wx]

First time I get to watch videos as soon as it comes out!! :D This information is great and thank you so much for putting your time and effort into this video. The content is just beyond expectations!! Like always, amazing work!!

[@luigibby4591]

This is an amazing and educational video. Your production quality is smooth and you're not afraid to add a personal touch. Thank you for teaching me something new.

[@samanthamerritt1718]

A Pink princess is fairly common in Canada. You won't find them in big box stores but they're in pretty much every independent plant shop and cost about 20 dollars.

[@ceitwolfe]

Where about in Canada are you? I'm in Edmonton & haver never seen one before.

[@pansymoons9536]

Similar in Australia, I've seen PPP in Bunnings before (which I think is equivalent to like, Home Depot in America?) they didn't really have any pink on them and cost about $50 AUD, but they're not super rare.

[@kaimay8657]

@@pansymoons9536 $50? More like $20 and half the time marked down to $5

[@CrazyPlantGuy]

Wow. So cool. Great video Kaylee and lots of info, definitely learned a lot. 😊

[@odd.fingers]

This video gives me How It’s Made vibes! I’m really excited to see the update on these guys 🌱🙌🏻

[@ameliagraham92]

Wow! Thank-you so much Kaylee. I have read about this process but I am a visual learner and found the reading a bit confusing. This demo was exactly what I needed to understand it. Please do follow up on how your process goes. I would love to try it sometime myself if I can only find room! You are so clever. I love your videos.

[@StaceyHerewegrowagain]

Great video. I'm just starting to dip into tissue culture. So much to learn. Thanks for sharing! I really appreciate your knowledge. It's so amazing how the process is done.

[@lisapettit7334]

Thank you! This was a very clear and easy to understand description of the process. Love that you are doing "kitchen culture" and I'm looking forward to seeing the results!

[@thepropagationstation]

At 5:27 there's a guy in a full bodysuit with an N95 mask, holding a beaker with some dirt in it and there's just a cut off fern frond sitting on top of the dirt and he's measuring the frond 🤣 This is the most hillarious stock-film I've ever seen

[@rhondafisher72]

Great haul, love the planter (she looks like a Sassy..lol) of course the cactus you put in her is so fitting.

[@krystynachen]

I can’t wait to try this. I already culture fungi so I am excited to be using my skills for plants

[@brittewater]

This is one of your best explanation videos yet. You continue to impress me with your quality work.

[@aquaroxity]

It's been a pleasure watching your channel and editing style grow into this wonderful professional informational plant videos!

[@gabbiesettlemire1587]

This was a very well done video. Even down to the visuals on screen and the background music. Your informational videos are seriously so captivating!

[@carsonianthegreat4672]

Any update on what happened to this tissue culture? It would be interesting to see it 4 years later!

[@plantcollectormelbourne]

This video was perfect and answers all the questions I had! Coming from a science background, the demo was soooo freaking sick to watch!!

[@caranorini1]

Wow i appreciate all the hard work going into this video it really shows in the quality & super informative!

[@chasingzs]

The content and quality of this video was top notch! Loved everything about it. Thanks for sharing what you learn with us!

[@ztszebra]

This is fascinating. Thanks for al your hard work to bring us the best content!

[@LifeRomanticized]

I feel like this was the best birthday present I got in a while! Wow Kaylee, you gave us a chance to sneak behind the curtains of plant business! 🥳

[@AranDeathly]

Seize the means of Obliqua production ✊🏽😂

[@PamsPrettyPlants]

Aran_Deathly 💚

[@AranDeathly]

@@PamsPrettyPlants You know where I'm at and what I'm about 😂😂😂😂

[@endor8witch]

even if they make lots of obliqua, you'll need to give it a very strict environmental factors for it to thrive. in her earlier video she did say the oblique she showed just is surviving. not growing. it needs a tropical-like environment still.

[@AranDeathly]

@@endor8witch Dude, I already grow extremely sensitive aroids and terrestrial orchids. All you need is a bell jar or a cheap terrarium. Not to mention the many millions of folks who live in warmer climates throughout the world who can easily grow these guys outdoors

[@coagulatedsalts4711]

@@endor8witch not a problem for someone like me in the middle east. the country I live in is a peninsula and so we have humidity 24/7/365. :) and wouldn't it make sense to unleash them to the masses who can't care for them? you'd get profit that way because they can't care for it.

[@LL-rb8wd]

Very interesting video! I really liked the way you presented this and demonstrated the tissue culture. Its the first time I've seen this and it was fascinating, thanks! I also appreciate the amount of work you must have put into this.

[@zetagundamzz]

This was so so cool to see! I kind of knew what it was, but didn't know how it worked. Thanks!!

[@matildaandersson4550]

I can see a lot of effort was put into this! Thank you so much for taking the time to give us such a thorough and high quality explanation!

[@maevek8624]

I've been WAITING for a comprehensive tissue culture video!!!

[@tomorrow3582]

Hi Kaylee, tha k you for your video. Could you let me know where you buy your MS Media in the UK?

[@juniper754]

Was there a follow up video ?

[@wxlurker]

This was really informative and the editing was very sleek, thank you, Kaylee. Cool to know that the transparency of the medium was in part due to agar, agar-agar makes a lot of delicious desserts too.

[@PeggysPlants]

Very interesting. I've always wondered about the tissue culture process. Thanks!

[@PlantsPotsWhatNots]

So neat!!! Thanks so much for this hun! I'm sure a ton of us wanted to know what tissue culture actually is, as we hear it all the time. I know I did! Love your face! 🥰

[@MonkeyNielson]

Thank you so much for this! I have looked into trying this at home and have read and looked at a lot of stuff on the internet about it. So happy to see it al step by step in a clear video :)

[@WindowofHappinessblog]

What a genius way a science can do! Glad to know rare plants do still exist in the tropics, in a natural method. However i don’t have a bad feelings with tissue culture as well.. What an informative post. Angie

[@YayangAgungSundawa]

Thank you so much , its very helpful for me to understand TC , 😍

[@ronald7872]

I think I got a new hobby during this quarantine

[@PamsPrettyPlants]

This video was so worth the work!! Super informative! 👏🏻👏🏻👏🏻

[@julia_in_hamburg3809]

Wow! This might be your best video so far. Thank you!

[@ralphchristianson]

This is an amazing bit of information about propagating plants. Since most plant people seem to have issues with just watering their plants and finding the right light, I do not think there will be a rush to do this. I find it interesting that there is another level above your local green house, just another secret corporation plot for our favorite hobby. Seems to be right up there with the pharma industry, so must be some big money involved and this year lots of people are helping to promote growing more plants. It is great to learn about this.

[@Appleton-WI]

Amazing process! Thank you Kaylee Ellen!

[@scubamage]

Hey! You can fudge an autoclave at home with a pressure cooker if you need (preferably one that can go up to 15 PSI). Just make the mix with the agar and solution in a mason jar, and put it in the pressure cooker to sterilize. I used to make sterile agar slants for home mycology experiments years and years ago. It may work a bit better than a microwave, assuming the high temps won't hurt anything you have in your solution. It's definitely not as good as a real autoclave though.

[@AlphaHenriksen]

You hit the nail on the head with this. I have found white wizard, pink princess and thai con monsteras in most large plant shops in Denmark, almost definitely all tissue cultures and certainly extreme cheap compared to a number of years ago. I look forward to three years from now when the devil monster or albo/mint monstera might get hit by the same process and the prices drop

[@Sheryl1026]

Loved this! I've heard of tissue culture but, never really understood any of it... thanks for making it so simple to understand! Great video! I really enjoyed it & can't wait for an update on the next step in the process..☺️❤️

[@mollysisboo]

Your hard work editing this definitely shows! Amazing video 👏

[@kahleiahetaraka6718]

follow up video would be amazing! love this vid!

[@debracancelliere4081]

So interesting ! I’ve always curious what was involved with tissue culture.

[@danielle4248]

I really am looking forward to some kinda follow up on this

[@AungWinHtutGH]

Thank you so much for your nice explanation! By the way, your presentation is great. Let me know how to make the annotation in videos, please.

[@thatscool1550]

this video was so dang helpful. thank you so much Kaylee!!!

[@jasonguanzon8967]

Honestly tissue culture in aquatic and emersed plants makes buying them much more affordable. Fingers crossed the trend follows for the houseplant market!

[@irisssmits]

Lovely video :) As someone who studied as a molecular plant scientist I learned how to tissueculture (and still often help in the lab). So there are some holes in your story for me. But I think for most people this is very well explained! One of the things that I do want to mention is that some protecols for commercial plants are published, they can easily be found online trough google scolar. A lot of universities do tissue culture and there is lot of information out here. The second thing I would like to add to you video is that tissue culture is based on the principle that plant cells are totipotent. This means that every cell can be reverted into a stemcell that in turn can give birth to a completely new plant. One cell is enough (there are exeptions offcourse) so a tiny piece of leaf can be enough. You can see this really nicely in Begonia where from some types you can cut a leaf into pieces and grow a new plants from each piece. This is one of the reasons why I picked moleculair plant biology as a major, its almost magical :D We often use hormones for reverting the cells into stemcells blobs (we call callus), and then move them to a medium with hormones to grow shoots, then its moved to medium that grows roots. So most of the steps you mention are quite on point :) There are some more tips I can give you to keep stuff more sterile (as keeping medium sterile is one of the hardest things to do in tissue culture). Let me know if you need any more information :)

[@KayleeEllenOfficial]

Yeah at the moment I'm learning via trial and error! I'd love to have a chat about it over an instagram DM or something!

[@irisssmits]

@@KayleeEllenOfficial offcourse! Happy to help. Iris.saskia on instagram, I'll send your page a message :)

[@annad7062]

Wow - what a cool visual. Thank you for a glimpse into the process

[@moto-thug]

Thanks. Fab job. I've done similar with mushrooms. I may give it a go 👍

[@AlasaSamu]

I work as a scientific assistant in plant tissue culture and cell cultures. So cool that you did a video about it

[@rootedinplants]

I love this video and all the hard work that you put into it!!!

[@Uneedmoremeds]

Wow Kaylee you did an amazing job with this video. The production was clean and fun to watch. Thanks for taking your time to bring us this great information. Stay healthy and safe my friend🤗👍❤.

[@h.r.9563]

This is such a fascinating area of biology. Wow!

[@conniecarmona1051]

i love how you explain things! ive started following you for a week now and i’ve learned sooo much from you!

[@toohotglass]

Great video! My only critique is, alcohol doesn't kill everything on contact. It kills on evaporation. You can decrease chances of contamination if you let the alcohol evaporate in front of the flow hood before proceeding.

[@VividVerse]

This is such a cool video. I was wondering about this and you did a wonderful job explaining it. Thank you! ♥️

[@emmiestenelooceramics]

Finally a kaylee Ellen video!!😍🌱❤️

[@tanya9kin]

This was so informational and done so well. Really.. my inner super geek is so satisfied.

[@victorabadio9169]

This video! I mean... Wow! Thanks Kaylee! Best KZbinr out there, plant-related or not.

[@Uchi.Chafloque]

Amazing video! Thanks so much❤️

[@arielbedonio]

Where to buy the media

[@donnaholmes1158]

Love your honesty.

[@JimMeeker]

Very Cool Kaylee! This is something I've been reading about lately and it seems like a lot of the equipment needed for Kitchen Culture is the same as Mushroom growers use. Especially the equipment for keeping things sterile. Tissue culture tutorials always leave me a bit hollow though as no one wants to give away their secret hormone formulas. I can't imagine chopping up the meristem of a super rare plant though. That would make me panic. LOL Gotta love the "Science people" stock footage!

[@ericatorres1080]

I love the plant community and all the videos but this is soooooo refreshing and interesting and so different for what I'm used to. Looking forward for updates on this interesting topic!!!

[@chrisrad216]

It's been 8 months. What happened? Are you going to produce 102? Thank you for your channel. A treasure trove of information.

[@hausgagag4923]

i would love to see an update on your attempt! 🌱

[@braedonclanton125]

Same!

[@wendysalamat7568]

yes please @kaylee ellen

[@camille5173]

Kaylee Ellen = The Beyonce of plant community = THE QUEEN

[@aliciaroxanne]

You have outdone yourself this is fascinating!

[@flowwithspirit]

i learnt something new today. thank you! i‘m glad i clicked on your video 😊

[@9044emily]

Any other health care professionals think nooooooo when she put her whole arm in with a jumper on 🤣🤣 laminate flow hood would push air over your jumper and onto the table, always bare below the elbow! Really cool to see though!! New subscriber just binge watching videos!!

[@kellygregoryco]

thank you! so well delivered. how did your kitchen culture experiment go? have you posted any update videos?

[@marcopaganelli6059]

Kaylee if you use microwave sometimes some spore will survive, an autoclave is basically a big pressure cooker so this is affordable to anybody, again nicely explained but to make home tissue culture more easy you should give some more explanations. In order to work in a clean area there are methods that don't need to involve a pricey laminar flow hood even if working in a hood is really a better experience (yet you can get contamination if your sterility protocol is not followed carefully even with the laminar flow hood, for instance you clearly touch the rim of the flask because you don't have longer tools, if your glove hasn't been previously completely sterilized or if by chance you extract your hand outside of the safe area in your hood and don't realize that, you will have contaminants on the gloves that may enter the flask so another important thing when possible is flaming) that said, if you use a glove box it is a cheaper way to get a sterile area, plus sometimes even just a plastic box on its side can do the job or simply just a flame because the area around a flame makes a circle of sterile air. Another way to get a sterile air is working over a boiling water carefully, the steam create the steryle area so working with slow movements is adviceable. Another system can even done in open air using the chlorine vapour sterilization method I explained on my channel that is good also to sow orchids seeds but can be applied to the tissue culture basics as well . So lets say hometissue culture is not impossible. And the people having rare plants at home should always be able to try to understand the simple protocols required by home tissue culture to try to get more copies of endangered plants.

[@Foreverfrys]

I saw a artical on tissue culture maybe 10 or 11 years ago in high times, since then I've asked around a bunch to many different people from many different backrounds very few have ever heard about it, even fewer have tried the process but this is the first time actually seeing this done really appreciiate it, very awesome to see this being preformed thank you a bunch for the solid information. Can you please do a follow up video I know I'd love to see it and I know others would too. Again thanks for the info and such a great page on youtube. love and light

[@dudedokduk3697]

so what happened to this tissue culture? 🤔 its been two years

[@LMEL756]

A true plant guru! Nice explanation KE!

[@katekupiec]

Hey Kaylee! Could you do a video of how to understand horticultural and scientific names and break down Technichal terms, eg: genus, species, cultivar, form, etc? Thanks and love your channel!