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G.I. Joe is the special mission unit operating under the control of the United States Military against evil Cobra organization.
#20 Comic relief Cobra henchman who likes to blow things up.
#19 an anti-environmentalist working for the Cobra, he brought Toxo-Vipers under his control to destroy and pollute environment.
#18 Cobra's evil scientist from the early days before #7 came along.
#17 An emissary of Cobra-La and #13, a half-woman half-reptile with super strength and reflexes and major villainess in GI Joe animated Movie.
#16 Cobra's bloodthirsty tank destroyer and master of explosives.
#15 Silent fighter pilot and Cobra's ace who is as mysterious as deadly.
#14 Cobra agent who became Cobra's new commander after "Cobra Civil war" event and thus he is major villain in IDW Comics.
#13 Leader of Cobra-La, ancient Himalayan kingdom that once ruled the earth. He's the main villain in GI Joe animated movie.
#12 Master of disguise, deadly assassin, the sister of #4 and connected to #8.
#11 A mercenary who works for the Cobra Organization as a master saboteur and demolition expert.
#10 Dangerous eye patch mercenary who has served many dictators and mostly the Cobra. Common secondary antagonist in GI Joe adventures.
#9 Acrobatic twins who often appear fighting against Joes but they also manage the Cobra's financial affairs.
#8 Dangerous mercenary biker gang leaded by #4, the most famous lineup is Buzzer, Ripper and Torch but many other members have joined this as well like Monkeywrench and Thrasher.
#7 The most infamous evil scientist in Cobra organization who has been involved in many evil things such as creating #5.
#6 Cobra's deadly ninja and master of martial arts, he's the evil counterpart of Snake-Eyes.
#5 Cobra's ultimate leader who was created from the genes of ancient dictators to rule whole organization (and the world) as their emperor when the Cobra officers grew tired of #1's failures.
#4 Deadly assassin, master of disguise, leader of #8 and one of the main villains of Joes who has a strange weakness for sunlight.
#3 Deadly, ruthless, aristocratic, beautiful right-hand-woman of #1 and lover of #2. Main female villain of GI Joe.
#2 Second-in-command of the Cobra and traditional supervillain in every way with the mask, deadly gadgets and high criminal intelligence, smarter than his boss #1.
#1 Who else can be number one than the leader of the Cobra and one of the most memorable villains from the 80s cartoons who never gets Joes.