Training: Carl Zeiss LSM 880 Confocal Microscope

Please watch our Introduction to Confocal Microscopy lecture before taking this training: • Introduction to Confoc...
A complete user training for the Carl Zeiss LSM 880 confocal microscope. This training takes the user from powering the instrument on through collecting 3 color confocal images with 3D Z-Stacks. This comprehensive training video is designed for Baylor College of Medicine graduate students, post docs and independent researchers utilizing the OiVM core's microscopy systems.
00:00:00 - Introduction
00:00:46 - Overview of the LSM 880
00:01:21 - Power On the LSM 880
00:06:02 - Mounting the Sample
00:16:31 - Configuring the Beampath
00:33:11 - Optimizing the Image
00:58:13 - Collecting a Z-Stack
01:02:03 - Shutting Down the LSM 880

[@jannamoen4370]

I've been looking for a tutorial that explains both the "how" AND the "why", and this absolutely did both! Fantastic video, thanks for posting!

[@JinxinGao]

I am totally cleared after seeing the tutorial.

[@haniekhorshidi8753]

The greatest training ever! Thanks

[@Shaq0112]

Superb tutorial. Thank you very much!

[@glendaoliveira5342]

Thank you for the great tutorial! It helped me so much.

[@OiVM]

Awesome! Glad it was useful for you.

[@KamalUddin-gr8qk]

The best !!!!

[@user-xe5wo6tn8u]

The best.

[@TheUdayachandrika]

Can you share how to change and keep the CO2 chamber for live cell imaging

[@carlh.6450]

Thank you for this helpful video! I have a question concerning the Beampath Configuration: How can I change the Detectors? Instead of Ch1, CHS1 and CH2 I can only choose between CH1, CH2 GaAsP and CH3. How can I set up the same detectors you showed in this video? I'd be extremely thankful if you could help me out with that!

[@OiVM]

Glad you liked it! Detector choice will depend on your system configuration. Some LSM 780/880 instruments are equipped with a single PMT instead of the 32 channel spectral array (ChS) in that second detector slot. Regardless, the order of the channels would be the same for the sequential acquisition presented. On your system use Ch2 GaAsP in place of ChS1 and Ch3 in place of Ch2. Ch1 remains the same. You will just rely on the upstream prisms for emission separation to Ch2 rather than the electronic selection the 32 element spectral array provides.

[@soothingmelodies6556]

How about taking DIC or PC please, Thanks

[@cleusaoliveira9788]

This tutorial is so great! Do you have a written protocol that I can print so all the students can follow? We use a shared equipment and all the settings are deleted when we exit the program. Is there a way to get the same settings when we go back to the confocal? Thank you s much!

[@OiVM]

Thank you Cleusa! We do have a PDF guide on our website: oivm.org/lsm880/#training As for saving your settings - you can either save them to the Experiment Manager at the top of the Acquisition dialog or you can load them from an image by clicking the 'Reuse' button from any CZI image taken with the same system.

[@cleusaoliveira9788]

@@OiVM Thank you Jason. The guide has some areas where the text is not very visible. I looked for the "reuse" button but I couldn't find it. Is it possible that we don't see it because of the version that we have?

[@OiVM]

@@cleusaoliveira9788 You can find the Reuse button in the 'Dimensions' tab located at the bottom of the image display.