Translation in prokaryotes lecture 2 | tRNA charging in protein synthesis

[@tirthankarpal2068]

This is the proper tRNA charging 🙌🏻.... loved your way of teaching.....a crystal clear concept has been made in my mind..... truly mesmerized sir🙏🏻

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@PratikPatel244644]

In 2:40 It's not ACC sequence.. It is read out as CCA sequence.... Coz the reading of codons are always from 5'-->3' direction...... But then also u r great teacher.. I follow most of ur videos #Shomu'sGenius......

[@diyabhattacharya1333]

"Adenylation" is the wrong term used, it should be "Adenylylation" .Both are completely different. Adenylation is transfer of adenine and Adenylylation is attaching of Adenine with amino acid. Its okay, we are humans, we tend to make mistakes. Good going. :)

[@prakatneupane2912]

i was about to comment the same

[@sandramusiyiwa1725]

Adenylation this is when adenine is added to something and Adenylylation this is when adenine +ribose +phosphate are added

[@tella5357]

@@plabanbhattacharjee4516 No it isnt dummy

[@poorviasthana1587]

@@plabanbhattacharjee4516 no intelligent

[@bhartiparihar]

Hi Shomu. The ester bond is formed between the 3' hydroxyl of the adenine from the tRNA and the carbon from the C terminus of the aa, not the Nitrogen from the N terminus. The N terminus should be available after the ester bond has formed.

[@Tariq5P]

Ester bond formation occur between lipid

[@krupasanghavi6881]

thankyou so much for all your helpful videos.. u seem to have covered almost all the topics in CSIR NET syllabus. its a great help..thankyou.. however i would like to point that in this video you say the N from the amino group acts as an nucleophile but its the oxygen from the COO- group (which is attached to AMP) which acts like the nucleophile

[@nadaibrahim1403]

😍😍😍 that is teaching. ........really you are the best .....

[@swetabiswas8066]

thank u SO MUCH FOR HELPING ME IN THE BSC COURSES. ACTUALLY I BELONG TO THE CALCUTTA UNIVERSITY WHERE SCORING IS VERY MUCH DIFFICULT BUT I THINK THAT UNDER UR GUIDENCE I'LL BE GETTING THE DESIRED SCORED VERY SOON.. THANK U SO MUCH DADA FOR MAKING THESE AMAZING VIDEOS WHICH ARE GOING TO HELP MILLIONS IN LEARNING. THANK U SO MUCH AND UR A GREAT TEACHER. LOVE UR LECTURES.

[@shubhamredekar3780]

Thank you so much sir Aapki vajise hamara subject jate jate bacha You are the real hero sir Such a great persnality I love sir

[@bikidas5029]

isnt it the lone pair of oxygen of 2 prime or 3 prime hydroxyl group of ribose of t-RNA which attacks the carbon of aminoacyl-AMP

[@jeliyaashish]

transfer of aminoacyl group to the trna, aminoacyl group attach at 2' hydroxyl group and 3' hydroxyl group of the terminal ribose by the class 1 enzyme and class 2, (aaRS) enzyme respectivlyand this reaction is called aminoacylation. amino acid attached to trna by high energy bond and the energy release by the hydrolysis of acyl linkage use in peptide bond formation. in this reaction one oxygen is removed from amino acid.

[@bmchicswati8425]

till now the best videos ever for studies...keep it up sir 👍 very well defined in a simple and easy way

[@shomusbiologyofficial]

+Swati Sinha thank you. Glad you liked my lectures

[@discriplinehelpusDas]

Great explaination

[@shomusbiologyofficial]

Thank you

[@archanahm3665]

Why shomu sir you dont comment on messages which tell you like you did mistake at some points...if you comment on it then it will be still more useful..otherwise beginners follow wrong information...and sir you are already a proved lecture .often we do commit mistake while teaching out of anxiety..excitement.its very normal..but that mistake needs to be accepted and corrected..there is nothing like ego issues i hope...mistakes are very easily ruled out those who watches

[@soumyaranjanmahanta9127]

Thank you so much 😊 Sir 🙏

[@shomusbiologyofficial]

You're welcome

[@arabindabiswas4670]

VERY NICE WORK SIR KEEP IT UP UR WAY OF TEACHING IS GOOD

[@bikidas5029]

you should delete this video and make a new one, and you are a amazing teacher no doubt

[@raghukul_junior]

Thanks alot you have been most helpful :) such a great video

[@shomusbiologyofficial]

Thank you.

[@leratoshasha08]

thank you so much i understand now God bless you

[@fathimaarafa2585]

always thanking you sir for helping to do some rapid revisions

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@SUBHAJIT-fox]

Ester bond formation occur between 3 ' OH group of tRNA and carboxylic group of amino acid not with amino group

[@kaustavsen1324]

such a great video

[@shomusbiologyofficial]

+KAUSTAV SEN you're welcome

[@laaarsas]

How does the tRNA select the correct amino acid with specificity corresponding to it's anticodon?

[@devanggondaleeya4321]

By the amino acyl tRNA synthase enzyme.

[@iwsv7253]

please guide more about recombination.. what r the functions of Rec proteins

[@shomusbiologyofficial]

Thank you. Please watch my recombination video

[@MrOnepieceadventure]

thank you sir! You are so great at explaining!!

[@kaziabdulmasud5284]

SORRY Sir I was thinking the bond forms between 3'OH group of sugar and -COOH group of amino acids (not NH2)

[@aboutstudies123]

Great video Sir

[@dbjrc]

thank you soo much u are a real life saver

[@akashvani3836]

But the 1st reaction is not carried out by aatRNA synthetase... it's aa transferase... I don't think that they are the same...Btw Very good explanation💛

[@shomusbiologyofficial]

Same enzyme used.

[@atahanersoy5168]

great job thankss

[@akankshagupta3026]

sir....it's rely u .....gladdd

[@mamuclasses3644]

Sir your lectures are great but in trna charging Amino acid joins to trna by its carboxyl group not by its Amino group

[@letslearntogether.4336]

Thankyouuu sir❤

[@shomusbiologyofficial]

You're welcome

[@neethuletha9684]

Thank u sir for this good lectures...

[@shomusbiologyofficial]

+Neethu letha thank you. Glad you liked my lectures

[@dxuan96]

Sir, thank you very much for the lectures! :) U save my life in my Biochemistry. God bless you :)

[@triptimehra5940]

It is the carboxyl group which is envolved..

[@Priyankakumari-so3jk]

I really didn't understand your explanations...can u plzz tell me what is structure of aminoacyl adenylate..and how it is removed by the t RNA ....and how tRNA attaches with amino acid

[@dan-simi6]

carboxyl group should bind the trna and the amine group will form the peptide during elongation

[@reshmasamanta4845]

such a great vdo

[@priyankeedutta808]

please make some videos on breeding methods of plant breeding ...please 🙂

[@ilovebiology3926]

Thanks sir ☺️

[@shomusbiologyofficial]

You're welcome

[@33sunandakuanr79]

Sir first the anticodon loop reads the codon on mRNA or first aa is attached to the tRNA

[@iwsv7253]

u r great.. thanku

[@shomusbiologyofficial]

Thank you.

[@aratrikade5612]

Dude this video is all wrong... Firstly it's "adenylylation" cuz AMP moeity attaches to amino acid... And the Alpha-NH2 gr of amino acids remain free,the carboxyl gr attaches to 3’-OH of CCA on acceptor arm of tRNA.

[@bio-learnerschannelforquer3873]

Yes

[@parameshvaranshothiga9665]

@@bio-learnerschannelforquer3873 m

[@Krish_demonslayeredits]

It is either 3'-oH/2'-OH

[@malaraju87]

It may be very silly doubt ,but pls help me Amino acid which comes and binds with amino acid acceptor loop of tRNA synthesized where Pls clear my doubt...

[@Krish_demonslayeredits]

The amino acid are consumed by us in the form of protein in our diet moreover few of them are also biosynthesized by us from the byproduct of Krebs cycle with some modifications.

[@malaraju87]

Thank you bro👍

[@srish2536]

🙏🙏💗💗🇮🇳U r a gem🌈

[@pushpadhruv2265]

3 prime end of tRNA should bind to carboxylic group of amino acid. but you said it is attached to to NH3 side of amino acid. how is it possible??

[@akankshagupta3026]

veryyyyyyy hplful sir thnkyu....

[@shomusbiologyofficial]

+AKANKSHA GUPTA you're welcome

[@madhavendrashukla5776]

Carboxyl group of amino acid binds to the hydroxil group of ribose sugar. Please check out this thing...bcz i think that's a wrong idea fir attachment of sugar and amino acid.

[@shittanshusingh1902]

First amino acid attached to t rna with the COOH group not with the NH2

[@sukhpreetsingh5603]

shomu bro plz make a video on lytic lysogeny gene regulation these cro c1 c11 xis prm pr they are so confusing.. i know u have a video on this topic but that is not much helpful. ..

[@bignwa6952]

great!!!

[@abhidhakela5019]

How many minimum no. of tRNA required in production of protein??

[@Priyankakumari-so3jk]

tRNA does not produce amino acid it only binds with it and carry it to the ribosome...1 tRNA will be required to carry 1 amino acid

[@kalpeshmori2123]

thank u bro

[@shomusbiologyofficial]

+Kalpesh Mori you're welcome

[@karthikparker8079]

I HAVE ONE DOUBT SIR ,PLEASE CLEAR ME WHAT IS THE ACC PORTION IN tRNA.

[@sudarshanreddy5115]

Tq sir

[@shomusbiologyofficial]

You're welcome

[@mariperez404]

my son has AARSD1 GENE we are looking for diseases it causes my son also has DEPDC5 gene also that causes rare epilepsy he is 14 yrs old any help is good thank you

[@theabhishekbhowmik]

your videos are good but sometimes you keep going in circles so please stay in the point.

[@letsdoit1262]

there are more than 20 tRNA in a cell, so there is not only one tRNA for one amino acid.

[@moonknight616]

What is fidelity of translation?

[@aratrikade5612]

Oh and there are not just 20 types of tRNA in a cell... What about amino acids that are encoded by 5 or more codons...

[@sandhyaindori6401]

Sir 50s and 30s will be 80s then why we calle 70s ???? Complex

[@kshitizadhyani4055]

It is adenylylation and not adenylation...nevertheless...nice explanation

[@hussainzuhajamz1420]

👍👍👍

[@dynogamer02]

where r uh from

[@shomusbiologyofficial]

Kolkata

[@Satya_Soach]

❤️❤️❤️

[@tanu6464]

☺☺☺☺☺

[@ambreennaz4682]

mujhe paper k hawale se kuch help chye plzzzz

[@mirthush]

Where is the trna origin

[@vinodrana1153]

Got

[@vinodrana1153]

I got it

[@abhisheklamkhade6289]

not understandable my dude !!!!

[@Bindu_08771]

Watching in2024

[@mukundaupadhyaya7861]

Bas 1bar Hindi ma v bol dijia sir

[@shomusbiologyofficial]

Sure

[@prashantchauhan8549]

yeah dude study urself .. properly before delivering the lecture .....

[@manuraj8889]

then can you create lecture videos and post it on the youtube? He is of such a small age and he presents every topic with crystal clarity.

[@aratrikade5612]

manu raj age shouldn't allow somebody to put out wrong concepts on a public portal, especially since people depend on his lectures and accept these explanations.

[@jayantbatra1838]

Lakadbhaga khi ka

[@mohammadwazid1934]

Bhai Hindi me bano lo

[@kalpanajaiswar5025]

Thank you so much sir 🙏😊

[@repalasravanthi283]

Thank u sir

[@shomusbiologyofficial]

You're welcome

[@Bindu_08771]

Watching in2024

[@shomusbiologyofficial]

All the best