Understanding Sequence Alignment Algorithms: with Needleman-Wunsch

[@kaidenwilliams7047]

I don't often comment on KZbin videos, but this was explained as clearly and succinctly as I could imagine. Thank you for making this video, it helped me out a lot in my understanding of the algorithm.

[@NicoMarveaux]

Also not a usual commenter myself, but was going to comment to say the same thing!

[@biobruh...]

Great video!. Had a tough time understanding Neeldeman-Wunsch, but I got it now. Thanks for the clear and structured explanation!

[@mattralston4969]

Really excellent! Succinct and simple to understand. Thanks for mentioning substitutions and affine scoring. That's a nuance that shows real mastery and I like your video!

[@Am-ug9np]

I watched like every other video on this topic on youtube, and not 1 other person mentioned the traceback matrix OR that we need to look for gaps in both x and y, for some dumb reason lol. I finally understand thanks to your video

[@verinen_runkkari5652]

Hi! At 9:51 when the video was fastforwarded you were getting into the matrix entry on which the row and column both corresponds to "C". Why was -1 added instead of +1?

[@othmanelamoumni3019]

I guess you should go back to 6:09, when you calculate from the diagonal Fi-1, j-1 you are adding +1 for match or -1 for mismatch. When you calculate from up or left (Fi-1 or Fj-1) you are adding G which in this case equals -1.

[@astratenebris1461]

great question i missed that too the first time, and great answer, clear and to the point

[@Periareion]

This was very helpful. Thank you, Hendrix!

[@aurel8872]

Thank you so much. I took bioinformatics, but at Biology level and we lack a lot of Mathematical and Statistics background at my uni - not to mention Informatics. We touch on these options in Blast but for me to learn I need to know how something works at least a bit. A lot of informatics and statistics info is very overwhelming but you put it very succintly so that even I understood.

[@alexkesin]

Great video! Explained this super clearly

[@sleepwright8378]

Thank you Professor, very helpful!

[@EgeBerkYurtkoruyanStudent]

Sir, you are a legend. Thank you.

[@RowingIsFun]

Good stuff! Thanks for making this video.

[@styxnexus]

Very well explained !

[@alimudarres1246]

Also, anyone else running into more complex or unequal lengths of sequences, there can be multiple solutions since your trace back matrix could have branches going multiple paths all giving the same score. Easiest would be to trace a path and read it from top left to bottom right to avoid confusion with where to apply the gap etc

[@vitortarghetta418]

Awesome! Thanks for the uploading this video!

[@Msyoutube38]

On the column and row where first A's match why do we pick to go(11.11) right side (2->1)when we can also move downwards on that backtracking matrix?Makes no sense to me..

[@nycoshouse]

hello, is there a meaning or applications to use a base 4 system and associate CAGT with 0123 ?

[@mememememememememe]

Great Video! Thank you so much 🙏

[@ishandvd]

Brilliantly explained, Thank you

[@busbusvlog]

Thanks a lot! This was very helpful for my studies

[@MultiSpiros123]

So good explanation !!!! thank you !!!

[@Rickyp0123]

Super clear and helpful. Thanks!

[@alecskriloff4250]

Saved my life rn

[@halafares333]

what if its a semi-global alignment ??? how cam i use this website ??

[@markphilipcastillo4029]

Great, thanks professor .

[@kenjidemesure1697]

nicely explained!

[@paulo_ebrd4127]

Merci beaucoup !!

[@qaziacademy3048]

Sir ur videos are amazing. Please i need ur kind help in Feature extraction. Please help me.

[@aurelienjeanneau5334]

Thanks !

[@caseses]

I have to thank you!

[@nicolasantonio8053]

Thank you!

[@dorotap3373]

yhank you!!

[@QanarIsDelirious]

Tough one :D

[@atenek9243]

Thanks

[@SubhanUllah-bc9wb]

Hello sir myre chennal pe alignment se related video hey accha lage to comment kigiye🙏😊

[@BryanJL]

thanks daddy

[@gschitz]

⥊ 771: 58 0: Good (if not good, then not zero) 0001: Uniqueness 001: Identity 002: Individuality 003: Variability 004: Diversity 005: Tolerance / Inclusion 006: Acceptance 007: Consonance / Harmony 008: 009: Transcendence 01: Awareness 02: Diversion 03: Sensing 04: Love 05: Grace 06: Reasoning 07: Ingenuity 08: Purity 09: Truth Seeking 1: Ethical 2: Problem-Solution 3: Situation 4: Abstraction 5: Expression Concrete 6: Why/Who/When/How/Where/What 7: Information/Code 8: Stability 9: Sanity 10: Network (Good Network, because zero) 11: Justice 12: Time/Spread/Dissipation/Easter 13: Death / Fact 14: Cognition 15: Agent 16: Reason 17: Discourse 18: State 19: Discipline / Control 20: Judgement 21: To Solve Problems with Ethics 22: Resistance/Endure Introspection 23: Consideration 24: Humor / Mental State 25: Behavior 26: Motivation 27: Cohesion 28: Dependability 29: Prognostic 30: Liberty Subject Wellness 31: Consent 32: Discernment 33: Response 34: Ideas 35: Showing 36: Option 37: Opinion 38: Decision 39: Optimism 40: Wellness 41: Attention / Idea 42: Critical Thinking 43: Acknowledgment 44: Free Love 45: Plan / Task Force 46: Purpose 47: Perspective 48: Character 49: Ambition 50: Good Expression 51: Manifestation 52: Support 53: Respect 54: Care 55: Peace / War 56: Pointing / Profiling 57: Tell / Counter-Terrorism 58: Invoking / Building 59: Proficiency / To Excel 60: Certainty 61: Identification 62: Qualification / Characterization 63: Inquiring 64: Conjecturing 65: Function / Role 66: Investigation 67: Arguing 68: Indicator 69: Scrutinity 70: Validation 71: Illustration / Knowledge 72: Construct 73: Explanation / Informing 74: Data 75: Materialization 76: Delineation / Instruction 77: Flow / Cadency 78: Share 79: Accountability 80: Personal Soverenity 81: Independency Constancy 82: Resilience 83: Established 84: Autonomy 85: Executing 86: Authority 87: Stream 88: Consistency 89: Boldness 90: Wisdom 91: Assertiveness 92: Science 93: Scrupulousness 94: Integrality 95: Composure 96: Blockchain: ⥊ (TRACE MARKER) 97: Anarchy 98: Veganism 99: Revolution 100: Liberation 101: Space and Time Peer-2-Peer 102: Decentralized Network 103: Financial Revolution 104: Production Revolution 105: Labour Reorganization 106: Needs Evaluation 107: Instruction 108: Personal Sovereignty 109: Resources Availability 110: Frankness 111: Honor 112: Distributed Ledger 113: Privacy 114: Abolitionism 115: Pride 116: Pacifism 119: Mission 120: Harvest 121: To Revolt 122: To Sort Out 125: Differentiate 126: Distinguish 130: Realization 131: Fulfillment 132: Regard 133: Perform 134: Address 135: Execute 136: Obtain 138: To Mark 140: Serenity 141: Idea 144: To Notice 148: Mindfulness 150: Good Character 151: Virtue 155: Honesty 160: Technology 161: Communication Systems 166: Causality 167: To Label 170: Knowledge 185: System Accessibility 181: Structure 199: The Mission 200: Sanctuary 209: Enlist 210: Resolution 211: To Engage 212: Secure 215: Mobilize 219: Annihilation 220: Contemplation / Meditation 221: Either Way / Alignment 222: Cooperation 230: Resilience 250: To Thrive 255: To Emerge 266: Correlation 300: Crusade / Jihad 301: Confront 309: Consensus 310: Cell Net 311: Agreement 320: Deliberation 322: Oportunity 330: Win-Win Energy Management 333: Abundance 340: Abolitionism 355: 360: Technology 373: Remote Killing 370: Efficiency 390: Economicity 400: Earth Population 410: Permaculture 430: Ecosystem 440: Fellowship 444: Direct Digital Democracy 500: Assumption 501: Leadership 510: Admiration 511: Honorable 512: Altruism 520: Proficiency 530: Acceptance 535: Denoucing 540: Appreciation 550: Diplomacy 555: Gathering 556: To Pick Up Someone/Something 590: Excellency 600: Organizator 620: Diagnose 660: Operation 661: Operator 665: Role 666: Method 676: Deduce 700: Constitution 710: Intelligence 717: Desing 720: Quantum Mechanics 733: Argorithm Explaining 747: Artificial Intelligence 750: Consolidation 751: Enlightment 757: Prompt 766: Plan 770: The Gear 771: File 773: Algorithm Recoginzing 775: Output 777: Pattern / Rhetoric 778: Development 780: Systematization 799: Revolution Algorithm 800: Providence / Heroism 801: Production 802: Necessities 803: Resorces 804: Energy 808: Renewable Cycle 810: Permaculture 820: Management 830: Logistics 831: Scheme 832: Reach 833: Demand 834: Stock 835: Distribution 840: Consumption 850: Labor 860: Human Resources 863: Assignments 870: Structure 880: Peace 888: Globalization 890: Needs 899: Conception 900: Transparency 910: Institutions 911: Emergency Call 930: Energy Distribution 931: Only One Global Currency 960: Blockchain 962: Traceability 970: Optimination 990: Inspection 995: Accountancy 998: Regulation 999: Implementation 1000: Militancy 1001: Nova Era 1312: Boycott 1042: Drug Liberation 1100: Liberation Army 1400: Unity 1500: Benevolence 1550: Charity 1807: Black & Yellow 2222: Paradox 3000: Freedom 3100: Triumph 3330: We All Can Live In Abundance 4000: Coexist 5000: Glory 5500: Greatness 6999: Mystery 7220: Unified Field Theory 8000: Sustainability 8999: Finitude 9997: Discovery 9998: Unknown 9999: Universe 10000: Existence . This is a numeric Matrix for communications purposes. . This can be used to convey meaning. Ex.: 6022: Good Reason to Resist. . Mathematical operators can be used to calculate meaning. Ex.: To liberate with a good force: 340. 300 + 40 = 340. . Logical operators to create statements. Ex.: ¬11: 13. If not Justice then Death. . Colors can also be used as information. - You can help build it.

[@gschitz]

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