Bacterial Identification Tests: Motility Test (Hanging drop method)

[@renish1531]

This video is very helpful i will recommend it to my friends . The motility test was my weakness but not anymore.

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@SatyamKumar-tn4nt]

Excellent explanation Now my concept is clear and I can give viva without any fear Thank you sir 😊

[@the_bioway]

I'm glad that this was helpful for you🙏🏼 Best of luck for your Viva👍🏼

[@ghaidaghaida3742]

Very helpful. In college we use the play dough instead of cavity slide. I did this experiment 4 times and I couldn’t observe any motility. So my teacher couldn’t give me the mark. I think that’s because we fix the lens on 100x power , I will try again with 40x power. Thanx

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@savvydeadpansnarker2733]

Superb sir

[@pavadaiarumugam9723]

Very clear explanation sir. Thank you so much

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@rithikmodi4468]

Hi direct second year students

[@BalogunRoseline-oj3zn]

Thanks for this wonderful teaching

[@the_bioway]

Glad it was helpful!

[@roshangaikwad7962]

“I like your video!”

[@the_bioway]

Thanks

[@subhdrasahu3273]

Wow ❤ Sir this was so interesting and helpful🎉thank you so much

[@the_bioway]

Glad you liked it!

[@ammarsayyed1635]

Awesome video sir

[@abhitoons5213]

Ham to fan ho gaye apke 🎉

[@the_bioway]

I'm glad that this was helpful for you.🙏

[@priyakotian1264]

Sir.. You have to off the bunsen burner flame

[@usmanrafilu7386]

But sir why the video procedure are not displayed?

[@santhosh30899]

Thank u so much sir the video was really helpful❤

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@Mr_humerus462]

Excellent.. Thank you🙏💕

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@vishaltelange8995]

I have a doubt When we have to observe slide under microscope .from Which side we can observe From cover slip or cover slip below the slide

[@the_bioway]

You need to observe from the side where the cover slip is placed.

[@sheraziz147]

Very informative video

[@the_bioway]

I’m glad that this was helpful for you 🙏🏼

[@sethambrose7583]

Sir is there any need of adding safranin in this procedure?

[@the_bioway]

No. Not required

[@ritwikghosh7743]

Thank u Sir. Great explanation. One question, can plasticine be used in place of petroleum jelly?

[@the_bioway]

I'm glad that this was helpful for you🙏🏼 Coming to your question... Yeah you can use Plasticine provided it should hold cavity slide and cover slip properly.

[@ritwikghosh7743]

@@the_bioway Alright Sir, thanks😄

[@rithikmodi4468]

Hi malhari how are u

[@rithikmodi4468]

I love microbiology

[@welearner4402]

nice video sir, its really helpful

[@the_bioway]

I’m glad that this was helpful for you 🙏🏼

[@riyapopat9934]

Thank you so much

[@the_bioway]

I’m glad that this was helpful for you🙏🏼

[@sanarahim782]

Wgat if there isnt any cavity , will we be able to observe bacteria mobility.

[@akankshamadavi8919]

Plz tell the principle of hanging drop method.

[@zahramohammedhamedalmahroo1548]

Incredible

[@babluyadavbabluyadav8536]

Thanks

[@rekhashinde7015]

Sir you took another cover slip but didn't applied on slide na 😕

[@kalanidhih749]

Sir , why we focuse the edge of the drop in this method ? 🤔🤔

[@the_bioway]

There is higher probability of finding motile bacteria due to oxygen concentration at edge

[@kalanidhih749]

@@the_bioway Thanks a lot sir 😊

[@christinahinton4538]

excellent. thank you Sir.

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@ritashreebasu9806]

If you open the cover slip with sample of bacteria in air for a long time that u do now then bacteria could be change their character and started to reaction with environmental organisms...how can do u this process... sir??

[@دوديبدر-خ6ج]

God bless you💙

[@eleven9701]

Sir Do we need to use the fresh broth for viewing motility assay ...or can we use the refrigerated broth also

[@the_bioway]

Freshly prepared one.

[@SandeepSir1310]

Nice explanation...

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@lalitpandey240]

Nice video

[@the_bioway]

Thanks

[@buddy7270]

Thank you so much sir ❤️

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@babluyadavbabluyadav8536]

Very nice 👌👌

[@the_bioway]

I’m glad that this was helpful for you🙏🏼

[@drx_prem_nayak143]

Nice sir

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@pharmed6914]

Hlo is it possible to view the border in 40x too or in just 10x.. My teacher keeps asking for that in observation.. I'm not really able to get it.

[@the_bioway]

At 40x or 10x you can see only the border but to see bacteria you need to go for higher magnification power

[@ab21234]

Sir we can see any type of bacterial movement through this procedure?

[@the_bioway]

Yes

[@aliyusaniado8807]

Its helpful

[@the_bioway]

I’m glad that this was helpful for you 🙏🏼

[@restlessmedicos785]

I am worry for the subject from whom this sample is taken😩😩😩😭

[@anushreekor1648]

Why did u use petroleum jelly only to coverslip?

[@the_bioway]

To make sure only corners of coverslip getting attached

[@sumaiyyahashmi6623]

But we are not dropping any thing than why its called as hanging drop method

[@the_bioway]

Because bacterial sample we have taken on coverslip is now in inverted position against the cavity side.... when we we observe it under microscope ... Which results into drop hanging from coverslip

[@zahraa6475]

Sir please can Answer me the heating of loop donot kill bacteria ?

[@the_bioway]

Heating the loop kills unwanted bacteria... So it becomes sterile (free from bacteria) and after cooling it...then it can be used to transfer bacteria of our choice....

[@zahraa6475]

Thank you sir god bless you

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@priyakotian1264]

No.. We have ro sterilize the loop.. We are doing many cultures and and we have to transfer many of the culture to cover slip.. So we have to sterilize it

[@msdohni9369]

Heating of loop kya huta hai mam

[@fathielzahra]

How to do this experiment with solid media

[@the_bioway]

You can refer following article for Motility Test using Semi-solid Media microbeonline.com/tests-bacterial-motility-procedure-results/

[@fathielzahra]

@@the_bioway Tomorrow is our microbiology lab exam. But I'm still confused about making hanging drop from a solid media.

[@the_bioway]

Hanging drop method doesn't use Solid Media.

[@arivusaravanan7327]

I have a question Why do bacteria move in edged

[@the_bioway]

Bacteria move towards edge due to high oxygen concentration at edge

[@arivusaravanan7327]

Tq so much for your certain help.

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@rithikmodi4468]

Hiii

[@medicos5649]

❤️

[@zarin1897]

💕💕💕

[@CC2k12]

does this method need heat fixation?

[@the_bioway]

No

[@rithikmodi4468]

Malhari how are u

[@komaltiwari1407]

👍👍👍

[@the_bioway]

Thanks

[@brookarsenault1259]

can I hear a cat in the background. I hope not.

[@the_bioway]

Not at all... 😅

[@dannyguzman5142]

cats are great

[@PiyushKumar04141]

How to report?

[@the_bioway]

Reporting is done as Motile, non-Motile and sluggishly Motile organisms

[@i96i9]

ايزي😂😂😂😂😂😂😂😂😂😂😂😂😂😂😂😂

[@Josephhell43]

Thanks

[@Munde0608]

Nice Sir

[@kiranbhagat789]

Nice video

[@the_bioway]

I'm glad that this was helpful for you🙏🏼

[@ayrabatool]

❤