Membrane Filtration Technique for Water Analysis (E. coli, Salmonella, Pseudomonas, Coliform etc.)

[@ucjakozicka978]

The explanation was clear, concise and informative, I learnt all I needed to for a project Im working on, thank you!

[@MicroChemsExperiments]

Thank you so much

[@MicroChemsExperiments]

Send email for get help/suggestions about your practical knowledge ([e-mail])

[@me..7885]

Can not get over how amaizing ur explaination is...all r grateful for that massive work 🌹🌹🌹🌹

[@MicroChemsExperiments]

Thanks a bunch

[@dshift2225]

This my job at Nestle :) Living the dream!

[@MicroChemsExperiments]

Great !!!

[@khushiyadav6723]

Are you from India?

[@BhavyaRecordsOfficial]

Can u tell me if there is any vacancy available in Nestle?

[@n4nishiiiii]

So you only perform water testing?

[@muralibond1373]

what's the package bro...

[@vsrpinternational3863]

Brilliant..Continue the journey of knowledge.

[@MicroChemsExperiments]

Thank you, I will

[@agboalfred4567]

This is beautiful and so easy to comprehend. Thanks a lot

[@MicroChemsExperiments]

You are welcome. Stay with us

[@FideliaAyemhenre]

How can I carry out serial dilution on water analysis for assessing fecal enterococci contamination using River water

[@01kanan]

Good 👍information and steps of Coliform Group E.coli found proper

[@01kanan]

Please mam red colonies are gram negative bacteria mostly.

[@prafull07prajapati83]

You are talented and delivers the lecture very smooth Thank you so much

[@MicroChemsExperiments]

Thank you so much. Stay with us

[@materialsandtechnology8956]

kzbin.info/www/bejne/q6jKhWlres6MZ7M watch this video of membrane for water purification

[@JayAcharya-fz4kl]

Please make a video on MPN in a similar manner

[@theconqueror24568]

Can you pls share with us the method of the most probable number technique (MPN) also?

[@MicroChemsExperiments]

Ok, we will make video on it soon. Stay with us.

[@ziangvo1877]

Thanks for the video. Can u show me details preparation for the negative and positive control plz?

[@MicroChemsExperiments]

Ok. We will make video later

[@gracefulheart7910]

If we cannot count the number of "blue colonies", & "blue & purple colonies" we have to do dilution before filtering. So in that case, how to do dilution ? And how to do calculations ? Plzz explain. Thanks.

[@MicroChemsExperiments]

For dilution: mix 1 sample with 99ml sterile distilled water. Calculation: Number of colony × 100

[@aishamuhammad9010]

Plz explain 🙏

[@Adidthya]

Thank you dear.Can you please mention the specification of filtration unit?

[@LunjapikaiHaokip]

can we use hot air oven instead of autoclave for sterilization?

[@safaafrin5391]

Please upload the video of legionella detection

[@Travel4happiness2601]

Very nice, I really like it. just one rectification. It says incubate the plate @35 degree but in the video it showed putting the plate in the hot air oven.

[@MicroChemsExperiments]

We are using the Hot Air Oven as incubator.

[@brianmarv]

Can this Buchner's funnel be cleaned with flamed methanol without being damaged or only an autoclave?

[@MdSaidalSaba]

best wishes from my water chemistry lab

[@ALISONSAMANTAAGUILARAMELLER]

Great! Thank you...But I would like to know if you worked with hydrophilic or hydrophobic membranes

[@FideliaAyemhenre]

And which selective agar will I need to use?

[@radhaanand1099]

For incubation they are showing hot air oven

[@aasthaashi0500]

Hello. Thanks for the video. It was nicely explained. I would like to ask that how will we determine the water quality using this filter membrane method and what are some disadvantages of using this method.

[@MicroChemsExperiments]

If pathogenic bacteria is found, means that the water is bad in quality. Only disadvantage is that it is applicable for water sample with low bacterial count

[@aasthaashi0500]

@@MicroChemsExperiments Thank you so much. Really appreciated.❤

[@Bharatpithiyalive]

Please suggested another techniques for purified water testing

[@foodloverss84]

Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzbin.info/www/bejne/jKClgICoi8d3r7c

[@materialsandtechnology8956]

kzbin.info/www/bejne/q6jKhWlres6MZ7M watch this video of membrane for water purification

[@anaispelleteret3123]

Is it normal to observe something at the extremities of the filter ? Are they growing colonies ? Or do some metabolites reacts with the medium ? Or is it something else ?

[@SauravKumar-hm9un]

Nice video....plz make a video on multiple fermantation tube techniques for fecal coliform .

[@nisasa4814]

yess agree👆

[@MicroChemsExperiments]

Noted the parameter. We will work on it soon. Stay with us.

[@sushantpatil6679]

​@@MicroChemsExperimentswhen you will upload it?

[@aishamuhammad9010]

So plz how do you calculate for CFU. If the colonies are 180

[@arzoosundrani1415]

Very nice video

[@MicroChemsExperiments]

Thank you

[@sghosh1234]

I have a query..Suppose E coli (blue colony) is present in 70 CFU and coliform(pink colony) are absent.Then what should be the report? Total coliform=70 CFU E coli= 70 CFU - is this to be written in the report? Please clear my doubt

[@liesdiebibelbruder420]

So how are the Membrane Filters Sterilised? They don't seem to be Sterile.

[@MicroChemsExperiments]

It is sterilized by Autoclaving

[@liesdiebibelbruder420]

@@MicroChemsExperiments do you put them in aluminium foil for autoclaving?

[@vaidehipatel2630]

Is for the fruit Slurry is the same.

[@gyss3349]

Nice.i. appreciating & thanks all of you for giving me scientific knowledge regarding to microbiology & Micology in general.

[@MicroChemsExperiments]

Our pleasure!

[@ewelina3817]

Hey, could you tell me why you "don't invert plates during incubation". If you have some references to this method I would be appreciate if you could share. Thank you.

[@imonroy1928]

Hi, I feel if we incubate it in an inverted position, the filter paper may fall off from the media side of the plate.

[@MicroChemsExperiments]

You shouldn't invert the plate because filter paper is on the surface of the media. Reference: Iso 9308-1:2017

[@abdelrhmanali4215]

⁠​⁠@@MicroChemsExperiments thanks for the useful info, but as in ISO 9308-1/2014: after filteration, place the membrane filter on the agar. ensuring that no air is trapped underneath, invert petridish and incubate at 36 *c for 24h!

[@mirnahazem3702]

is it true to sterilize the filtration units in oven?

[@alicemori7327]

Hi, i have a question? How do i sterilise the agar medium since it can't be autoclaved?

[@MicroChemsExperiments]

No need to sterilize it. Just dissolve the media as per the instruction mentioned in the label or catalog of the media

[@KaarlisBaumanis]

Which Agar you would suggest for counting colonies at 22 and 36 degrees?

[@abronexportsskjain5295]

Well explained do you have any video colony counter working

[@MicroChemsExperiments]

We dont make video on instrument. In future, we will make video on different instruments. Stay with us.

[@MicroChemsExperiments]

We used colony counter in coliform count. Take a look

[@enviromantallabreport]

cant you use M -Fc agar LES for E.coli and M Endo Agar LES for total coliform organisms???by seeing this i can feel that results would be very hard to take

[@arima6210]

What agar plate that you used to measure the e coli

[@MicroChemsExperiments]

Chromogenic Coliform Agar

[@fadilanwar.official]

What is the name of this tool? Where we can buy it?

[@mehedihasanrabbi342]

Mention the oil content test procedure

[@MicroChemsExperiments]

Which sample?

[@mehedihasanrabbi342]

@@MicroChemsExperiments bleaching earth oil content. Vegetable oil

[@MicroChemsExperiments]

We already uploaded. Video link: kzbin.info/www/bejne/aGXUiImCopybntk

[@Bhagat.creation01]

This video is amazing mam, but I have a question, please reply. Actually, you told that E coli will appear blue in color on this media, but it is a gram negative bacteria. Then it should appear pink in color. Please tell me why this happens.

[@kinetomagnetic]

Thanks, one question: if the filter paper is not inverted while placing on agar, how the filtered microbes present at the top side of filter come in contact with the media?

[@MicroChemsExperiments]

Microorganisms absorb nutrients from the media through the semi permeable filter paper

[@kinetomagnetic]

@@MicroChemsExperiments Thanks!

[@keepgoing6630]

Good introduction. Can we put the filter paper into the liquid media in the flask after filtration instead of the Petri plates for growing the bacteria?

[@MicroChemsExperiments]

Yes. You can.

[@namitaarya3517]

In this viedo you have told that incubate the petri plate without inverting it ,but in ISO 9308-1 it there to invert the petri plate. Which is correct please make it sure.

[@MicroChemsExperiments]

Filter paper is on the the culture media of the petri plate. So the plate should not invert

[@AppolloniahNhire]

Hiw do we incorperate enrichment of the sample into this process?

[@MicroChemsExperiments]

Enrichment is not needed and not possible

[@vaishnaviraotokla8758]

Can we do different filter membrane filteration for the same solution??

[@MicroChemsExperiments]

You can

[@nadiaariba5118]

Thanks for explaining

[@MicroChemsExperiments]

You're welcome

[@miftaamalia4995]

Do you know what is the composition of chromogenic coliform agar media? Could you give the tutorial how to make it?

[@MicroChemsExperiments]

We know the composition but making in local lab is difficult

[@shravanis1755]

Is there any method to sterilize the membrane filters if they are not sealed?

[@MicroChemsExperiments]

If the filter autoclavable, then autoclave it to make it sterile

[@likeleah]

Thanks for the video.. can u explain and do a video on water sampling , normally checking for water quality in Industries

[@MicroChemsExperiments]

We will make video later. Thank you

[@sangrammore3985]

How could you use filter paper without pre wet

[@MicroChemsExperiments]

Try as shown in this video. It will work

[@rakiborrahman1368]

Perfect deliberation...👍 Thank you. May I have your gram negative strain photographic analysis under Florence Microscope please Dr. R.Rehman

[@alicemori7327]

hi, i have a question. does the filtration appratus need to be autoclave for each sample? Thank you

[@MicroChemsExperiments]

Yes

[@lalitasaini544]

Amazing experiment 🙌

[@MicroChemsExperiments]

Thank you. Keep supporting us.

[@dheerajtiwari920]

Very informative Video ....

[@MicroChemsExperiments]

Glad you liked it

[@lilianas9702]

Hi, I have a question about colonies on CCA chromogenic coliforms. Can we extend the incubation? Sometimes yellow colonies appear after 24 hours, which become pink with longer incubation. In this case, are there also coliform bacteria?

[@cryptusxx]

Thanks for the explanation. But can this method be used for contaminated food?

[@MicroChemsExperiments]

No. This method is not for the food sample

[@cryptusxx]

@@MicroChemsExperiments i see. Can you suggest what method can be used for food sample?

[@rejinaprajapati2571]

you say, for the preparation of the chromocult coliform agar, we need to dissolve 5.5 g of dehydrated power of agar, but you had written 2.55 g of to dissolve in 100 ml distilled water. Can you please clear me my confusion?

[@MicroChemsExperiments]

Ops sorry, we did mistake while saying. It should be 2.55g for 100ml.

[@sanjuktaghosh2886]

How the colour has come in bacterial colony?

[@MicroChemsExperiments]

Everything is described in this video. Watch the video again

[@shahidnawaz126]

excellent information. can you help me with CFU count? im doing testings on non sterile cotton

[@MicroChemsExperiments]

Surely I can help you. Please write in details.

[@theophilust9736]

Nice video ! Please can this technique be used for testing of Ecoli,Coliform,salmonella,pseudomonas and staphylococcus organisms present in sanitary pad. And if yes will it involve just diluting the sample in water ?

[@MicroChemsExperiments]

Yes. This method can be applied after diluting your sample in sterile distilled water if the expected microbial count is low. But if the expected microbial count in your sample is high, then you have to follow different method as I have already uploaded for enumeration of E.coli, coliform & salmonella from different sample. Please find those tests from my channel.

[@foodloverss84]

Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzbin.info/www/bejne/jKClgICoi8d3r7c

[@narendrakondamoni2246]

what is the Water testing guideline

[@MicroChemsExperiments]

Many guidelines can be found for water testing. Like American Water Association

[@shravanis1755]

If Colonies are growing outside the membrane that is below the membrane ,then what is the problem Mam?

[@MicroChemsExperiments]

It indicates cross contamination. Don't count them during result interpretation. only count colonies on the filter paper

[@shravanis1755]

@@MicroChemsExperiments thank you

[@redweasleypotter1127]

Thank for this video. So informative and another technique is added to my list

[@jandecoole5687]

Thank you great bexplaination

[@MicroChemsExperiments]

Glad you liked it

[@jimfanahvinpg5944]

Is it good topic for msc project?

[@jibantaraahongshangbam157]

Membrane filtration technique for water samples using chromocult agar I observed some transparent colonies apart from pink and blue colonies. So, I want to ask what organism is that transparent colonies. Can u explain.

[@MicroChemsExperiments]

You can use differential media and then conduct biochemical or serological experiments to confirm those colonies.

[@FayazKhan-gr1mx]

Can the liquid still go thru the filter without using a vacuum pump?

[@MicroChemsExperiments]

No. Water can't pass without using vaccume pump

[@Spring_rolls]

What is the active ingredient that is responsible for giving color to colonies?

[@MicroChemsExperiments]

Different media have different ingredients and give different colored colonies. Please study about media composition in Google

[@knowledgeuptocorner5863]

Nicely explained...

[@MicroChemsExperiments]

Thanks. Stay with us

[@tweety6878]

At how many count of coliform indicate water potablity?????

[@MicroChemsExperiments]

Zero coliform per 100ml drinking water

[@krishnachaitanya414]

Can I repeat the exact same process for Salmonella but replacing chromcult Agar with XLD and keeping all the steps same? Also how to calculate the number of colonies for it? the same way?

[@MicroChemsExperiments]

Yes you can, and the calculation will be the same.

[@krishnachaitanya414]

@@MicroChemsExperiments Thank you so much!

[@krishnachaitanya414]

i could not find XLD, so using Salmonella Shigella Agar now, hope it works in the same way.

[@Salmankhan-rj7vs]

Which type filter you're use

[@MicroChemsExperiments]

Everything is described in this video. Watch the video again

[@mansoorahmad490]

can we use VRBA/EMB for Colifrm/E. Coli & PDA for Yeast/Mold?

[@MicroChemsExperiments]

Yes you can

[@KomalpreetKaur-os6pb]

GOOD EXPLANATION:)

[@MicroChemsExperiments]

Thank you.

[@gladwelchepkemoi6249]

Is the measuring cylinder sterile ?

[@MicroChemsExperiments]

Yes.

[@redvelvet191]

I have question. For membrane filtration technique, it is advisable to filter 3 different volume. Do i need to sterilised the filtration unit in between filtering 3 volume or i can filter 3 different volume using the same filtration unit?

[@MicroChemsExperiments]

Sterilisation for 3 times is best but you can sterilise only once for 3 volumes of sample as you are taking same sample.

[@redvelvet191]

@@MicroChemsExperiments thank you so much for your answer

[@MicroChemsExperiments]

@@redvelvet191 you are most welcome

[@sulochanasinthanaithuli1936]

sulphate reduction anaerobic bacteria test (water) vedio upload mam plz

[@MicroChemsExperiments]

Noted

[@eyadomar00]

how much do the total test parts cost?

[@MicroChemsExperiments]

Varies with the type and brand of the used media and equipment

[@theophilust9736]

Thanks for the video Can you please do for testing for presence of yeast and mold in sample using this membrane filtration technique

[@MicroChemsExperiments]

The same procedure will be followed except DRBC agar plate should be used replacing the Chromocult Coliform Agar and incubate at 25 degree Celsius for 3-5 days. Total fungal colonies on membrane filter is the total yeast & mold.

[@theophilust9736]

@@MicroChemsExperiments and preparing the yeast and mold using distilled water ! Does it involve using peptone water ?

[@MicroChemsExperiments]

@@theophilust9736 Using normal physiological saline (0.85% NaCl)

[@theophilust9736]

You guys are awesome ! Love that you are willing to help I am trying to set up a lab for sanitary pad and I need agar to buy I will be testing for the presence of ecoil&coliform, yeast&mold, salmonella,staphylococcus aureus, pseudomonas aeroginosa, total viable count. Obviously I now know using membrane filtration is easier but knowing the appropriate selective agar for each is where I need help. I know for ecoli/coliform from this video and for the fungi through your response to my question. Please list the agar for salmonella, pseudomonas euroginosa,staphylococcus aureus and total viable count . I appreciate you

[@MicroChemsExperiments]

@@theophilust9736 Buy XLD agar for Salmonella, Nalidixic Cetrimide Agar for pseudomonas aeruginosa, Plate Count Agar for Total viable count and Mannitol Salt Agar for Staphylococcus aureus.

[@khoirulrozul9240]

Can this method be used for food samples such as sugar and others?

[@MicroChemsExperiments]

No. This method is applicable only for Water sample

[@deshdeepak098]

Mam we can use alternative M-indo broath.

[@MicroChemsExperiments]

No.

[@shahrilizat740]

Can i ask you?, Which regulation that you follow?? APHA or As??

[@MicroChemsExperiments]

ISO 9308

[@muralibond1373]

as per which standard u have performed these tests..

[@MicroChemsExperiments]

ISO

[@sathisdas5465]

No need to autoclave the medium?

[@MicroChemsExperiments]

No. No need to autoclave

[@alameenallu8639]

Is it .22 or .45 micron filtr??

[@MicroChemsExperiments]

0.45

[@fiacreishimwe7275]

Thank you, I understand it clearly.

[@MicroChemsExperiments]

Thanks for staying with us

[@materialsandtechnology8956]

kzbin.info/www/bejne/q6jKhWlres6MZ7M watch this video of membrane for water purification

[@qatetrapilpl3630]

Is this filter paper sterile?

[@MicroChemsExperiments]

Yes

[@sandhyaranjith427]

For heterotrophic count which culture media is used?Nutrient Agar is enough?

[@MicroChemsExperiments]

Yes. Nutrient Agar.

[@sandhyaranjith427]

@@MicroChemsExperiments Thank you. One more thing,For Fecal streptococcus and sulphite reducing bacteria which culture media is used?

[@MicroChemsExperiments]

@@sandhyaranjith427 Bile Esculin Azide Agar is used for Fecal streptococcus amd Tryptose-sulfite-cycloserine (TSC) agar is used for Sulphite Reducing Bacteria

[@sandhyaranjith427]

@@MicroChemsExperiments Thank you so much.

[@MicroChemsExperiments]

@@sandhyaranjith427 You are welcome.

[@joshuabansah7366]

Thanks for video

[@MicroChemsExperiments]

You are welcome

[@Jawaab01]

Subscribed because if this! ❤

[@MicroChemsExperiments]

Thank you. Stay with us

[@Bhabita-k8q]

Nice video

[@MicroChemsExperiments]

Thank you

[@Maheswair]

This media used in pesudomos

[@Ankitsingh-bq9bu]

Thank you so much mam

[@MicroChemsExperiments]

Most welcome 😊

[@sharyanazir9850]

Amazing.

[@MicroChemsExperiments]

Thank you

[@panggogi348]

thank you

[@MicroChemsExperiments]

You are welcome

[@chinmoymandal7858]

Voice very sweet

[@MicroChemsExperiments]

Thanks a bunch

[@thegandeeman]

I remember when this stuff was part of my continued education studies for licensure, I'm now to screwed over and drunk to care. Maybe I need methamphetamines

[@TR_Conqueror]

Thanks

[@kalyana679]

I love your voice

[@cook.king0844]

Nice❤❤

[@MicroChemsExperiments]

Thanks 🤗

[@foodloverss84]

@@MicroChemsExperiments Electrospun PVDF-carbon membranes preparation for water filtration. Pls watch our video kzbin.info/www/bejne/jKClgICoi8d3r7c