Vitamin-C (Ascorbic Acid) Analysis Using HPLC_Sample Preparation (Part-1)

[@SonooDixit]

V.nice

[@dr.ajaybarman7041]

So nice ... Very helpful video.. 🤗👍

[@MicroChemsExperiments]

Thank you

[@Wardi_lovers]

Very useful video 👍

[@MicroChemsExperiments]

Glad you think so!

[@kishorregmi2265]

please reference the protocol so that we can cite it

[@saravanans101]

Hi ma'am i have one doubt your using c18 column it is phenomenox make or any other make suppose if it is thermo scientific make is your using,im using phenomenox make no issues or any issues please clear my doubt

[@farahadra3395]

How to analyze vitamin D3 in a pharmaceutical preparation on an HPLC device on a C18 column

[@titelmegu9632]

Is this sample preparation is ok for vit c estimation using iodometry titration ?

[@MicroChemsExperiments]

No. This is applicable only for the analysis using HPLC

[@nadiatulhusna5943]

what if the sample is in juice form? how much it will be used?

[@MicroChemsExperiments]

This method is also applicable for juice.

[@ao-kq2iy]

please I want to know that the method is conducted by ISO standard??? My English grammer even is weak. I hope You can get it. I hope I can receive your answer soon. Thanks.

[@MicroChemsExperiments]

It's completely fine in understanding your question. No, this method is not made by following ISO.

[@aymenogkush]

Thanks for creating this channel, very helpful !! Please could you make a video about the analysis of BOD, Ammonia and total Phosphorus in water

[@MicroChemsExperiments]

Noted. We will make video leter. Stay with us.

[@lyndamezali7452]

Ascorbic acid is not vit C. This acid is just its external core. Anyway, thanks for the excellent explanation

[@MicroChemsExperiments]

Stay with us

[@LabAnaysis1385]

Plz defined de ioned water

[@MicroChemsExperiments]

In a simple definition, it is free from any kinds of ions.

[@LabAnaysis1385]

@@MicroChemsExperiments difference distilled water and de ioned water

[@MicroChemsExperiments]

@@LabAnaysis1385 Please take help from google.

[@woloabel]

(On Monday of May 15, 2023). On the Matter of High-Performance Liquid Chromatography (HPLC) and Sample Preparation (Herein Ascorbic Acid [Vitamin C]): 1) The Analyte however is not Pure or sometimes even known. I reckon this is merely a didactic Video. 2) I would probably be curious how much AIDS protein and HIV Nucleic Acid is Disseminated in the Air just as it is Transferred to the Population via the Services these Subjects have Proclivity in (All so-called professions) Lawyers, MDs, Architects, and thereafter how much is directly infused into Humans by these Thriving and Proliferating, Non-Human Chemical Terrorists (Politics Aside, is it not a Human Right Assault and Violation we ALL have in Common?). 3) Anyway, just a Thought-Hypothesis (With Ample Research and Investigation Play but without much Resources thereto). Keep science beneficial and utilitarian. And most important SIC SEMPER TYRANNIS. MDx, Es gut ist aber es auch muss sein rein und besser (am Besten Heiligest ist). Heil!

[@hammadjutt8616]

which sample is there at 3: 12 I mean from where you are extracting vitamin c

[@MicroChemsExperiments]

From fruit jam

[@valeedck8738]

Can I perform analysis of vitamin C in milk also using same method?

[@MicroChemsExperiments]

Yes. You can

[@nipunshapnil2663]

Can i use this sample preparation technique for determination of monosodium glutamate by using HPLC

[@MicroChemsExperiments]

No. This method is only for vitamin C

[@saliksiddiquee3254]

✔️✔️✔️

[@MicroChemsExperiments]

Thanks for your support

[@gayathryganesin712]

Thank u for the videos it’s very useful for us can make video abt vit d,total sugar ,total acidity by hplc

[@MicroChemsExperiments]

Noted. You can determine total sugar accurately by this method: kzbin.info/www/bejne/aZ-ud6aiq8-lirc We will make video for HPLC method later. Stay with us.

[@MeriemCHAOUCHE-xf7qg]

Thanks for the videos, could you do a video about diclofenac found in wastewater samples by HPLC

[@MicroChemsExperiments]

Noted.

[@anusreesobhanan4100]

Is this method applicable, for finding ascorbic acid content in plant extracts?

[@MicroChemsExperiments]

Yes.

[@ramakantmagade8820]

What's the source or reference of this method?

[@MicroChemsExperiments]

This is a laboratory developed method collected from multiple international journals.

[@sundayyua4203]

Thank you so much

[@MicroChemsExperiments]

You are welcome

[@surjitdeka7092]

Can I get the methods in pdf form and any other reference for this method please

[@MicroChemsExperiments]

No sir. We don't provide any document

[@surjitdeka7092]

@@MicroChemsExperiments ok thank you

[@toveivarsson247]

Hello! I am currently working on a scientific essay about vitamins, and one of the things that are included is an quantitive analysis of c-vitamin in an orange. So my question for you is have you used any scientific report regarding the methods of this video? And if you have is there any chance that you might be able to link the reports so that I have an reliable source which I can refer to in my essay.

[@MicroChemsExperiments]

Thanks for commenting here. This method is developed using several international publications (journals). But I can't provide you the links at this moment. Sorry for that. Search journals and try to match this procedure so that you can use them as reference.

[@johnimmanuel1774]

Kindly upload the video link for hplc analysis of the same.

[@MicroChemsExperiments]

We are already working on it. Will upload soon.

[@aswinkumar8016]

Part 2 pls I don't see any for the part 2 in vitamin e and a sample preparation also

[@MicroChemsExperiments]

Will upload soon. Stay with us.

[@tarik7849]

Hi .can i prepare ascorbic acid injection from effervescent tablets. Thanks

[@MicroChemsExperiments]

No idea

[@jasminesenevirathna2714]

sorry, I can't find the part 02 in the description as mentioned in the video

[@MicroChemsExperiments]

We are working with it. Will upload very soon. Stay with us.

[@MicroChemsExperiments]

Part-02: kzbin.info/www/bejne/Y16mdmefrsdlqNk

[@jasminesenevirathna2714]

@@MicroChemsExperiments thanks alot!

[@MicroChemsExperiments]

@@jasminesenevirathna2714 you are welcome

[@jasminesenevirathna2714]

@@MicroChemsExperiments Hi, is it alright to do the sample preparation day before the HPLC Analysis?

[@aezzygaming2385]

Please upload vitamin B

[@MicroChemsExperiments]

Ok. Noted.

[@chemmuhammad8471]

up

[@MicroChemsExperiments]

Thanks