We Upgraded Our Microscope!

Differential interference contrast is not a microscope, but rather a method that enhances contrast, and thanks to our new microscope we are able to share some amazing DIC images with you!
Get our new socks and coloring book here:
store.dftba.com/collections/j...
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Twitter: / journeytomicro
Facebook: / journeytomicro
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Instagram: / jam_and_germs
KZbin: / @jamsgerms
Hosted by Hank Green:
Twitter: / hankgreen
KZbin: / vlogbrothers
Music by Andrew Huang:
/ andrewhuang
Journey to the Microcosmos is a Complexly production.
Find out more at www.complexly.com
SOURCES:
www.microscopyu.com/tutorials...
www.microscopemaster.com/diff...

[@JamsGerms]

Thank you so much, everyone, who made the DIC possible for us! We have some gorgeous footage for you coming in the next episodes! -James

[@domm3361]

We love to share this journey with you :D

[@noeldenever]

Thank you for sharing your labor of love with us. I'm out of words to describe what a joy it is to watch your work ❤

[@covodex516]

I could've given you the DIC all the time, whenever you wanted. You just had to ask. But well, now a multitude of people gave you DIC together. Kinda dirty, but im happy you can enjoy the DIC now.

[@IgneousGorilla]

Have fun with your DIC my dude

[@ianbcnp]

Thank You James, the images are absolutely stunning - I enjoy every episode and look forward to whatever you are going to find to show us next :-)

[@wanderinguser7665]

Wow that's amazing! Looking forward to more DIC pics!

[@paraphidd]

Underrated comment

[@jeffgoldblunt]

Dic flicks

[@Robert_McGarry_Poems]

Second!

[@Freakhealer]

Naughty

[@levi12howell]

DIC vids bro

[@petruraciula9056]

I love how Hank uses a soft voice. It's like he dosn't want to disturb that tiny but huge universe.

[@INRFan]

Yesss

[@Blue-xc7qc]

I kept thinking that this guy sounds like Hank Green, and I finally figured out it is him because of this, haha.

[@iamanidiotbut5523]

It’s almost like it’s the first time he’s reading the script

[@NOMAD-qp3dd]

Yea he makes you feel like your crouched in taking turns looking through the microscope together, it's perfect.

[@padkirsch]

@@Blue-xc7qc holy shit that's EXACTLY what I was thinking loll

[@LordMoonie]

My mom loves microorganisms, they're her favorite thing to paint! So I showed her this video, and i came back about 5 minutes later to her crying! She said she was happy because she'd "seen the world". Thank y'all for making this moment of pure joy possible.

[@ireallyhatemakingupnamesfo1758]

What the hell, you can see the texture of the membranes. It’s astounding a) how incredible the contrast is for this footage, and b) how much that improves the experience

[@simonrodriguez4685]

It makes it different, the other two seasons are some of the most beautiful KZbin videos ever. We’ll see, it’s too early to tell. It looks fascinating, but better? Color photographs aren’t better than black and white, they’re different. More contrast allows for appreciating finer details. But what about the interactions of different organisms I wonder?

[@ac.creations]

@@simonrodriguez4685 color photography is objectively better. More information = more better

[@ireallyhatemakingupnamesfo1758]

Simón Rodríguez you’re absolutely right, but it stirs up wonder in me the way that a new perspective on something amazing always does

[@campkira]

technology.... those thing will die with just normal soup...

[@benrodir2]

going beyond all the childish jokes, I love how that changes everything from a flat object that just seems to move somehow, to a 3d living creature. Best upgrade yet

[@Imupinta]

It's not Flatland afterall!

[@mooseknuckle8334]

Hahaha childish jokes? Welcome to the interweb.

[@TheRealFlenuan]

but 2D > 3D

[@nathanlevesque7812]

@@TheRealFlenuan only if it's squared into 4d

[@10010011001100110101]

3d(ics)?

[@SnootyFarkleboob]

"If you've ever taken an optics course, you will remember how terrible that was." I've never felt so seen.

[@DrunkenUFOPilot]

In college, the prof who was going to teach Optics suddenly went to another university 2000 miles away. An optics engineer at a local company was found to fill in. He knew optics well, of course, but was a terrible teacher. So yeah, optics was terrible.

[@ricardocabrera8344]

OMG So many nightmares rigth there. hate it the course with all my might.

[@sasdagreat8052]

@@DrunkenUFOPilot So even the original professor wanted nothing to do with the course.

[@DrunkenUFOPilot]

@@sasdagreat8052 LOL, but no, I think he was offered more money, as he was the author of one of the more widely used first year physics texts.

[@anthonymorales842]

@@ricardocabrera8344 What was so bad about the course. Rationalizing the physics or the math involved? Never taken it.

[@arthurshawn9177]

Awesome upgrade! Suggestions based on curiosity: an episode on all body fluids, one about the germs living on our skin, one focused on the surfaces we touch daily, one on public areas, etc. Let's see if germophobes got it right 😊 So I dropped my sandwich on the floor, please show me the extra bacteria it collected. I didn't refrigerate the milk, or I left my pizza unde the bed (cliché). Don't forget to wash your hands! A series based on our daily life, discovering what we don't see but it's always there. 👍🏻

[@BossOfAllTrades]

Yea kinda cool I wouldn't freak out as we have our own microdefense machines living within us

[@GetThePizza]

"Dic Microscopy" I'm not going to watch it if you're going to insult me.

[@Ed-zv8ot]

lol

[@SkinsFirstGeneration]

Really advanced microscopy techniques needed to be developed to study your... Sample

[@Boogers32150]

He’d need an electron microscope to render that, my friend.

[@clarencetse]

*Shows up on Recommended Vidoes* I feel personally attacked

[@Elitecommando501]

@@SkinsFirstGeneration There's no advanced microscope yet to render such nano objects.

[@snaaail]

“If you’ve ever taken an optics course” Yes! It was the best class “Then you will remember how terrible it was” oh ok never mind

[@AyushBahuguna]

"Optics was a mistake." - Isaac Newton

[@snaaail]

:(

[@CucumbersSC]

RIGHT! Not even my area really but my opticts/classical physics course was really good and I learned a lot

[@mohithraju2629]

Same here, optics class was one of my favourite classes

[@sortof3337]

Leo Hultén mine worst. We had a tobacco chewing professor

[@ColinTimmins]

Wow, what a difference the new microscope has made! Thank you to all who contributed.

[@aishahwalker7503]

Omg this is amazing, these are the coolest microscope images I've seen. All the little organisms look adorable and I love it! Can't wait for more amazing videos!!!

[@waterunderthebridge7950]

The only DIC pics you’ll never get tired of

[@Christians_outdoors]

That’s what she said

[@Suchega_Uber]

8:25 Probably my favorite image. I love the colors for one, but I also like how the solitude of the amoeba gives it a sense of vastness, as if we were viewing some secluded part of outer space, rather than something many, many magnitudes smaller.

[@bignasty389]

It's incredible! I immediately came to the comments section to see if there was any discussion about it to be had. A little nebula, or a space ship floating slowly along its way; its shimmering lights decorating the void with something busy and hopeful.

[@Candesce]

I came down to the comments to say that this was by far my most favourite clip as well, for all the reasons you've outlined. It's beautiful.

[@Decodeish1]

Oh, I just commented the exact same thing and then saw your comment. It's simply amazing :D

[@zantus1438]

I loved that part

[@vartika_aga]

this is the most glorious comment section ever 😭💖

[@krisanthonysilveira8244]

Journey to the microcosmos is on a completely different dimension itself. The script, the music, the footage and the crafting have a deep spiritual essence to it. and my it hits you like a BRICK when you high! It takes me back to the exact moments when I would culture cilliates. Thank you journey to the microcosmos

[@diegocaballero59]

5:18 this is the sickest and dopest image I've seen from a Microscope

[@JasonSubers]

With the change in lens bringing a new perspective, I love that you didn't miss the opportunity to bring the analogy home and make it relevant and real. You guys never fail to deliver.

[@mixer0014]

So, You say even more amazing content is possible? I didn’t see that coming.

[@XstraightjaketmanX]

Neither did they, until they got the new microscope in

[@iloveeveryone8611]

“i want a DIC microscope!” Looks up price “Never mind”

[@kwinzman]

How much?

[@TheWalkingAmongTheDead]

@@kwinzman The Olympus BX53, which seems to be the one used here, costs more than 8 thousand dollars. I could be wrong, and instead they are using another one that seems to cost more than 30k.

[@earlspencer7863]

Try godove.com they do industrial and scientific equipment auctions. Bought a couple of rotovaps from there cheap :)

[@kwinzman]

@@TheWalkingAmongTheDead Thanks!

[@sntslilhlpr6601]

@@TheWalkingAmongTheDead Sounds in the ballpark, but that is a wide range. I think the fact that they had a tech come out and fix them up definitely puts them in the upper part of it, but I really don't know and have very limited experience with this stuff. But you gotta know that when manufacturers start saying "call for pricing" you know that shit is expensive... I used to work on some Zeiss stuff and while I wasn't on the optical side, the shit I worked on ran about 100k, but that's FDA approved type shit which balloons the cost compared to just some badass microscope a consumer might want to buy, and the price of optics has come down as china gets better at it (I have a Chinese scope on my very expensive rifle, call me a traitor if you want to but there it is). But this isn't even including the imaging equipment they've been using all this time which I'm sure is not cheap. I'm guessing they're mid 5 figures all in at this point on the equipment. And I'm glad they're investing in their operation and they definitely deserve more subscribers. I was on patreon before they took off but I just can't justify it in times like these.

[@rebeccaadamson5972]

"If you've ever taken an optics class then you will remember how terrible that was" HANK I'M DYING

[@rotifer]

*Serratia marcescens is red,* *Dinoflagellates are blue,* *I missed the Microcosmos,* *And I missed you too.*

[@gregoryfenn1462]

I just pooped you out. Sorry bro

[@pattheplanter]

Looking good, Rotifer!

[@ChaosMagnet]

We missed you too!

[@nahushmodak1003]

I was legit searching for this comment.

[@HimanshuSharma-oy9ss]

Daphnia-tely.

[@lemont3a]

watching these videos really help me calm down when im feeling stressed or anxious, thank you JTTM!!

[@thewoodweldingfabricator9300]

There is a certain zen to this.

[@makaimaukahasopinions848]

Same

[@mikeljmcphee]

Same here.

[@browneyedbitch62]

The talk about how the DIC microscope works was very interesting. But the pictures are fantastic you can see the cilia moving in exquisite detail. And watching it on my larger HD TV it blows your mind. The colors are so much more intense. I am glad that you were able to get your new DIC microscope. Keep up the good work

[@dudeb5610]

"DIC MICROSCOPY" Me: *My asian insecurities intensifies*

[@evergriffith221]

INTENSIFY

[@tasnifrahman2486]

Bahahahaha

[@mamons_2339]

Yeah i have this condition too, i think.

[@kdvr766]

Lool

[@Yetipfote]

You deserve a free pair of algae socks.

[@fritz6462]

You can’t imagine the amount of happiness you people bring me. I love all of you

[@NewMessage]

Literally awestruck here. Been a long, long time since I've felt that... * tips an imaginary hat *

[@NewMessage]

(my daughter just added that she could tell, because I didn't make a DIC joke)

[@semaj_5022]

My mouth was hanging open watching this whole video. I feel you, it is hard to truly blow me away anymore with so much at our fingertips on the internet to desensitize. This was truly astonishingly awesome though

[@raoulduke7668]

I really love the fact that you explain the different microscoping techniques so accurately ! Really shows how much you love microscopy and your fanbase! Looking forward for more content :D greetz from germany

[@PhillipAmthor]

Your voice is more than satisfying to listen to. And im happy for james with his new microscope!

[@FingerinUrDaughter]

this looks so good, people will mistake it for high quality physics engines.

[@keithleracc]

"Dic microscopy?" Reminds me of what my ex girlfriend said to me...

[@aspektx]

Well that was a DIC move on her part.

[@mooseknuckle8334]

You should have listened to your mother and left it alone.

[@Ed-zv8ot]

lmfao

[@Elkoreshzulkarnain]

🥀🍆

[@csn583]

@@mooseknuckle8334 Don't play with your DIC microscope or you'll go blind.

[@mihaipredescu]

This is a great job, images are so crisp and motion so.. truely reproduced that I can enter easily with my mind's eye inside this microcosmos!! Congrats, guys!!

[@albertusdrostable]

This could potential bring up unhappy memories of school. But for pure entertainment: PERFECT! I love it!

[@elizaalmabuena]

looks wonderful guys congrats on the new microscope, James

[@robertholquist627]

Congratulations James, Hank, and team on the new equipment/techniques. We appreciate all of your work and respect your passion.

[@backpacker3421]

I've been subscribed since this channel started, and watched every episode. This.... this was the first time I ever expanded the video to full screen. That was truly wonderful to watch. I look forward to many more with the new scope!

[@13mjunky]

What a beautiful view!!!! Thank you! Well done! I'm blown away!

[@thepoofster2251]

I will always remember the emotion I felt the first time looking through a telescope. It amazed me that I was actually seeing light that bounced off Jupiter. To be able to get that experience TWICE in the microcosms makes James a lucky man. It honestly makes me a little emotional knowing that you guys got to make this means year with this microscope

[@edwarddewolf3392]

Looking forward to some amazing footage using this microscope, keep up the AMAZING work !

[@gregkral4467]

Those images are indeed some of the very best I have ever seen, thank you so much for all of your work, fascinating, just pure fascinating.

[@deliafunk9810]

This is so amazing! Really springs to life, makes such a difference! Thank you

[@Apostate_ofmind]

my mouth literally hang open for five good seconds. I almost cried. I didnt think i could be so excited for a microscope. Thank the stars im taking the right path, studying biology, and every excitement at new tech, new discoveries, new creatures, reminds me of it every time more. I cant wait to see more. I always felt microscope made me feel kinda blind, since contrast and details are often hard to focus on, or capture correctly in the field of view. This video made me feel like i put a new prescription of glasses on (and i would know the feeling).

[@peytoia]

Ohhhh this footage is stunning! I completely understand why James was getting emotional over it, I teared up a little too. I’m so happy for you all for making it so far and doing so well, you definitely deserve it. Keep up the good work and here’s to another three seasons of doing what you love in the future!

[@aclearlight]

Bravo! So glad you are doing this work!

[@typhoon9945]

I can't wait for the new episodes with these amazing pictures. Thanks to everyone who contributed.

[@Kram1032]

dang. So, the first episode of JttM was breath taking. It continued to be that, of course, but at the same time, the first WOW is always going to be hard to beat. This beat that.

[@TheFuturistTom]

I’ve been watching Journey to the Microcosmos for a while now! I loved their content! As such, I made my own sci-fi/futurist channel!

[@chestuk68]

I'm totally awe-struck; thank you for this amazing footage! I'll definitely be watching more. And the merch! Those socks??? Yass

[@thekewpie353]

Learning about new things is often exciting but I like that sometimes it can be a quiet wonder. Thanks for making videos that have incredible content and also have this meditative quality, it is a much appreciated pace change.

[@OwariNeko]

Nice! A new microscope!

[@matthewbaker7513]

60 FPS is seriously a huge upgrade as well, having these both happen at once makes the series unbearably beautiful

[@JimWilbourne]

Great upgrade! And the story told was great too. When I saw that the video was nearly 10 minutes, I didn't know how you'd fill the time, but you did a great job with that. Make sure to let us know what your next upgrade goal is so we can help you get there!

[@HayTatsuko]

You've upgraded your microscope and our enjoyment of the things you'll be sharing with us in the future. Thank you, very much. The sharp new DIC images in this are fabulous. I can hardly wait to see more!

[@emrazum]

You guys should make a 10 hour compilation of microbial footage so I can use it as a TV screensaver at work

[@shellypatterson6519]

DarlEng I would pay for that

[@tatfung]

They would need to have a motorised XYZ stage and tracking software with autofocus to keep the images and microbes in the field of view, life support system for those little cells, maybe 20-30hours shooting in timelapse and tons of luck

[@emrazum]

@@tatfung or just compile all of the existing videos in one big thing

[@coyote-bird1801]

The last time I was this early the sun was a deadly lazer

[@meatvortexgaming7134]

🎵 Not any more there’s a blanket 🎵

[@TheYahmez]

kzbin.info/www/bejne/rqamn2urrZeamNU For those who have not seen and really aught to. 🙃

Congratulations!! Looks amazing!!

[@chimedemon]

Man this little channel makes me feel so happy, even with how you guys express your excitement at getting new equipment to see more sides of the the hidden world. I'm excited for what you'll find and teach us in the future! :)

[@padawanelite]

I loved this channel before. Now it's just reached a whole NEW level. Truly amazing sir. 👏👏👏

[@simon_far]

Everyone is talking about DIC jokes, and all I can think about is Disseminated Intravascular Coagulation

[@feeltheeffect85]

Same

[@thebiglightbulb1457]

Sounds painful

[@ooooneeee]

Ikr

[@wombat.6652]

@@thebiglightbulb1457 Yes, and can be lethal frequently.

[@nycboriqua90]

Amazing footage, & I see even smaller germs or what ever and it's amazing 👏

[@springbird2265]

Detailed and amazing.Thank you.

[@TheRogueWolf]

Cripes, for half a moment I believed I'd actually be able to feel the texture of that _Stentor_ at 1:12.

[@TheMcKenzieHaus]

Me too! Just breathtaking

[@Deeraise]

It's like microscopic corduroy.

[@RadioRoxx.FM_90.1FM]

I never seen such quality

[@repeatdefender6032]

THIS IS AMAZING!!! Dude, we’ve gotta find a way for you to work with IMAX, that would be utterly mind-blowing!

[@mariatherese3353]

This makes me teary eyed 🥺 the organisms I only see in books and pictures are moving beautifully and clearly. 👏🏻👍🏻

[@TheDanknugg]

Absolutely beautiful details now, can’t wait for the new content from this microscope

[@MannFace51]

The DIC we didn’t ask for, but needed

[@DIVERGEsound]

This is extremely high quality content.

[@annefoley6950]

I may have actually screamed when I saw that first Stentor, looking like those little plastic tubes filled with water and glitter, that you can stick your fingers in ... but moving?! Gosh that is beautiful

[@malaikamillions]

You (all involved) bring so much wealth to my reality, for which I am enormously grateful! Thank You.

[@mohithraju2629]

question: All the dic microscopy pictures I have seen till now were in greyscale, why do we see colour in James' video? My gut feeling says: Higher resolution photos under dic don't have colour while lower resolution ones do. I still don't understand why some pictures of dic are in greyscale while others have colour.

[@MegaLuc1997]

Though I'm not sure about this answer, I think your intuition is right, higher resolution cameras usually don't have colour because the colour needs to be detected by RGB pixels and therefore you would be "wasting" pixels on generating colour. James probably uses a lower resolution camera that is able to detect colour, whereas most scientists go for the higher resolution cameras in greyscale, since colour doesn't contain much actual information about the sample. Though it sure does make a pretty image ;)

[@ceoyoyo]

Most very small things are essentially transparent. They modify the phase of the light but don't absorb any, and this change is invisible. DIC is designed to turn some of those phase changes into amplitude changes, so you can see them. For things like that there isn't really any colour, and a grayscale camera can give a higher resolution because it doesn't have to capture the R,G and B channels. For really high resolution microscopy you might also use blue or violet laser light instead of white light. The multicellular organisms on this channel are bigger and have a lot of colour, so filming them with a colour camera is definitely worth it.

[@companerger9416]

ceoyoyo That was helpful to learn. Thank you for sharing.

[@LandoCalrissiano]

@@MegaLuc1997 I imagine you could have different filters that filter for red, green, or blue light, take greyscale images through those and combine them in software. This is done in astronomy for a different reason but same principle can apply to give high resolution color images.

[@MegaLuc1997]

@@LandoCalrissiano definitely! But your resolution will still go down, not in space, but in time! Probably not much of an issue for dead stuff or diatoms, but definitely for moving things like the stentor. Another solution is to split the light path twice and then using the color filters, this would require you to adjust the sensitivity of the cameras, but it is probably the best solution, allowing high resolution and high speed. Though it would also be a lot more expensive since it requires three cameras.

[@laurahill9643]

I'm just over here, grinning like a loon while watching.

[@FriedFreya]

I'd get chocked up, too, seeing as that seems one of his most used study mediums... It's emotional, seeing even closer, more than likely. They're so... tiny... but they do so much with their little lives. Amazing. I can't wait to see more.

[@AwesomeShotStudios]

Fantastic images! Congratulations on the new optics.

[@nicolasleonard4658]

His voice is trying to hypnotized me. When I woke up, i was in another planet full of virus, brrrr

[@Monk-E]

Nice

[@lopkobor6916]

Nice

[@kylemathew205]

This is the first video I have ever seen on your channel... 0:23 in and it's an instant like and subscribe. That moment when you find a whole KZbin channel of content. This is amazing.

[@lianav707]

I don't know what Thrills me more... the Clarity and the beauty of these microorganisms themselves, or the plethora of tiny creatures running around the microorganisms. It is truly mind-blowing!

[@yaraihan]

7:51 Also It Means "More Than Meets The Eye"

[@someoneyouknow525]

Absolutely amazing. Literally looks fake it's so good! If I owned this, I'd never leave my house lol

[@dustypidgeon]

Great stuff everyone! Thanks for the amazing content I can't believe it just keeps getting better and better!

[@68FCchannel]

thank you for creating this channel

[@tp6335]

Next milestone: scanning electron microscope. To bad it kills everything you put in it....

[@ElectricityTaster]

what if we put in a cell taken from Prince Phillip?

[@tp6335]

@@ElectricityTaster lmfao

[@kum6865]

I sometimes wonder, do microorganisms get scared when they see a big round eye in the sky?

[@raphaelachristalynelleaudr8372]

hmmm, interesting

[@David_Kin]

They dont have any feelings, theyre microbs

[@johndor7793]

@@raphaelachristalynelleaudr8372 tf are u smoking, they dont have eyes

[@rubytuby6369]

It was strange how some of the organisms look like they were investigating other nearby creatures, almost like they were thinking.

[@alejandroe.zunigasanchez259]

John Doe They do tho Lol flat worms, Tardigrades, copepodos, they all have eyes

[@madeinusados2808]

This is one of the most amazing things i've seen in my life. Never thought i'd live to see something like this. Thank you

[@silviam.7195]

Differential Phase Contrast Microscopy was at first invented by the german scientist Fritz Zernicke (+1965) in 1938 further developing his Phase contrast method using circular and elliptical polarisation. It´s an amazing video....:D.... Greetings, Silvia. :D

[@12e444]

>stentor at 650x and i'm sitting here bouncing in my seat with excitement because i'm like "WAIT We can see them BIGGER? with MORE DETAIL?"

[@rutvikrs]

"That's what she said"

[@jpdominator]

Absolutely crazy detail. Looking forward to seeing more!

[@guri311]

Really fantastic and very impressiv! Thanks vor this Video and others!

[@mybocks3]

Half the comments: Interesting content! Other half: *G I G G I T Y*

[@gwyn.]

*Love your DIC vids James!*

[@khamaSenin]

Love how a real microscopist shows us the diversity of the microcosms. Rather than just looking at bacteria

[@Scatyricon]

Super cool. Can't wait to see what else this new equipment reveals.

[@nightcoder5k]

Wow! I've never seen anything like it. Thank you!

[@turneramas]

F...ing amazing!!!!!!! Love the new microscope!!!!!!! Congratulations 🎉

[@dariocruz3158]

Kick ass vid. Solid channel. Congrets

[@leojnardrapsing9577]

THANK YOU FOR SHARING THIS VISION!!

[@sweetsue4204]

I’m mesmerized. James.... that was emotionally moving. 💕

[@yakfarang8366]

Spectacular..... many thanks.

[@manthighs69]

im glad the incredible concepts and truths in these videos can be closer expressed though the beautiful imagery now :)

[@merbst]

This is the best DIC I have ever seen. 😉 Such beautiful Interference Contrast illumination of Algae.