What does it take to kill a bacterial spore? Petri dish timelapse

An experiment showing different environmental conditions bacterial spores can survive through. Petri dishes were incubated for 24 hours at 37 degrees Celsius. Each time-lapse shot is 24 hours long.
Drums by David Maya
Guitar by Sci-inspi

[@francescobertelli847]

"Everything was killed!" *LOUD HEAVY METAL MUSIC PLAYING*

[@Torch314159265]

Made me think of a Doom level for a moment.

[@dandanthedandan7558]

Reminds me of the hydraulic press channel intro

[@mr.waffentrager4400]

I was going to say that...

[@wartman1006]

Death note almost

[@aceshadowins1310]

Torch314159265 more like death note

[@schizophreniagaming1187]

"Everything was killed!" 0.01% of bacteria: *Laughs microscopically *

[@cringeusername9961]

The rest 0.0001% that you haven talked about:am i a joke to you

[@dandanthedandan7558]

@Kaya Beautymantra It can also be the 0.0001 that is not included in the 0.01

[@brian5619]

DANDAN THE DANDAN that makes no sense. Was your math taught by your gym reacher?

[@kevinjhoncadiz2342]

@@brian5619 I know right?😂 We're talking about odds here and he makes no sense😂

[@dandanthedandan7558]

@@brian5619 No, I meant like if there's a hundred boxes, you can have three groups: 10 red boxes, one green box, and the remaining 89 that is unclassified. That's what I meant. Also, stop being rude you kid.

[@dandanthedandan7558]

Oh come on using an autoclave is like bringing a nuke to a knife fight

[@wormwoodbecomedelphinus4131]

Increased thermal transfer due to higher pressure. Yeah. Not a fucking chance.

[@NotSoCrazyNinja]

There is a non-zero chance at some pathogen evolving to survive such harsh conditions. Very unlikely, but the chance is there. There already seems to exist living things that can survive an autoclave.

[@dandanthedandan7558]

@@NotSoCrazyNinja yeah it's called "next to impossible"

[@tolentinoalexam.7262]

@@NotSoCrazyNinja I mean it's true though, some bacteria CAN survive harsh conditions. They're called "Archaebacteria".(Please don't call me a nerd for this because I literally forgot what it was called though my teacher just taught this some days ago.)

[@flamed_bars7299]

Tolentino, Alexa M. You’re not a nerd. I already knew that, but good observation

[@kingseekerbackup3085]

E. Coli: *Infects humans and probably causes death* Also E. Coli: *Dies to almost anything*

[@anduro7448]

*The glass cannon*

[@PauaP]

@@anduro7448 Hay thats a pretty good name for E Coli.

[@elie464]

Well to be fair a human would probably die in the same conditions. They're just like us~

[@albapantheratigris6071]

I mean that’s why people at the CDC jump off of buildings when an E.Coli outbreak happens. Wash your damn hands, especially after you touch your poonanards, before you serve someone at a restaurant.

[@kingseekerbackup3085]

@Jesus Christ whats your point?

[@CannotBeVerified]

Interested to see the effect of alcohol or antiseptic cleaning products

[@Jeercrul]

I can't provide the exact statistics because that would require indepth research into that, but I can tell you that even 70% alcohol is not trusted to sterilize surfaces, only disinfect. However at 90% ethanol bacterial tends to encyst and not die.

[@CannotBeVerified]

@@Jeercrul woahh but how to clean and kill tho. Crazy

[@sci-inspi]

Yea they will survive through a lot of cleaning products.

[@Jeercrul]

@@CannotBeVerified trust me, if you've been disinfecting (not sterilizing) surfaces all this time and have not fallen sick all the time, there's nothing to worry about. Less than 1% will hurt you, and there are many beneficial bacteria out there that help us too. I know how scary it seems that some bacteria do not get killed permanently, but this is more of an issue of labs and hospitals. Like the video shows, autoclaving is a routine in hospitals and labs where they are working wih the more dangerous strains. Nothing to worry too much about.

[@elalogar7340]

​@@sci-inspi Bleach included?

[@NoobAlike]

Autoclave: I am inevitable ...

[@Allplussomeminus]

Even the name sounds badass

[@sundeww]

guy with chainsaw: and i... am going to destroy you

[@Inidentificado]

And I.. am... Prion Man

[@rahmatbanua7009]

Inidentificado i see what you did there. Protein man

[@austinpowersasmaozedong]

and I'm pepsi man

[@6Twisted]

Damn, 2 minutes of UV light was enough to kill most of them where as 10 minutes of boiling hardly affected the spores. I didn't realise UV was so damaging.

[@Kanal7Indonesia]

All hail the UV light!

[@kellynolen498]

@@jomsies sunscreen is uv black face....... .........._|prove me wrong|_

[@sharpen193]

@@kellynolen498 well first of all sunscreen is white

[@kellynolen498]

@@sharpen193 well its simple stuff is white because it reflects the visible light its uv blackface because on a uv camera or if you could see in uv it would show up as black

[@bongotoucan7913]

Kelly Nolen r/wooosh

[@ritacortez3883]

"Time to bring out the big guns! THE AUTOCLAVE!!!..." And the change in the music... AMAZING!!! EXCELLENT WORK!!!

[@sci-inspi]

Lol I had to make it match the video.

[@GoldenizedElite]

@@sci-inspi solo tabs? didn't know you also played metal lmao

[@thraximundar2974]

@@sci-inspi evidently shazam recognizes the music too.

[@snowstarsparkle]

@@thraximundar2974 LMAO, I didn't know it could do that! And what a nice song name too XD

[@Kenmanhl]

I knew the autoclave would work the moment the lid was closed and it showed the autoclave with a confidant smile

[@sunisol3058]

The guitar riff sounds like the death note soundtrack

[@RicardoMontania]

my thoughts

[@user-vz5md5tm4u]

bass only

[@heyweykrutcorbin5005]

Ikr

[@kgun3434]

I was thinking the same exact thing!

[@viejodegeneradoculoroto4111]

Yaaa

[@monsegeek]

If B. Licheniformis is so resilient and given the fact that it can also infect humans, I wonder if it's antibiotic resistent as well.

[@pulvenberg1709]

After some time... Most propably

[@ryanchuabowen2045]

@@changweihsu bacteriophage*

[@Liamb2179]

I found a paper where they tested the susceptibility of 90+ strains of B. Licheniformis in korea, most of the strains they found were on average susceptible to some antibiotics, but interestingly were naturally resistant to two types (chloramphenicol and streptomycin) and there were a lot of strains that appeared to have acquired resistance to erythromycin. So on the whole it appears to be mostly resistant to some antibiotics but not at an alarming rate, however any bacteria can become resistant to any antibiotic over time. I also learned that it likes to live in fermented soybean based food which are very common in korea! Apparently infections from it are quite rare. Ref: Do-Won Jeong et al. Antibiotic susceptibilities and characteristics of Bacillus licheniformis isolates from traditional Korean fermented soybean foods, LWT-Food Science and Technology DOI: 10.1016/j.lwt.2016.10.001 (disclaimer: I spent maybe 5 mins on this so might not be accurate, also this was just the first paper I saw and I've never even heard of this journal so take this with a grain of salt)

[@monsegeek]

@@Liamb2179 Wow, really nice of you to take the time and look it up. Thank you! And yes, totally true that any bacteria can develop antibiotic resistance over time. Current projections predict that by 2050 resistent bacteria will cause more human deaths than cancer. That's just terrifying, if you ask me.

[@minepose98]

@@monsegeek It's just slightly less terrifying when you realize that this is in part due to cancer deaths being replaced by resistant bacteria deaths. Or does that make it worse?

[@simunator]

omg they just won't die

[@Alexander99602]

Autoclave : I would like to introduce myself

[@lynx5327]

besides E. coli that got fucked real hard

[@Dover939]

@Lynx fuck you ecoli get fucked

[@Just_Disco]

Autoclave : I’m about to end this man’s whole career

[@tedchirvasiu]

...until *heavy metal music starts playing*

[@natanezra8589]

B. licheniformis: why do i hear boss music?

[@deborahol]

Where is this bacteria mostly found, is it a very common bacteria?

[@illegal_juicebox]

*T H E A U T O C L A V E* [evil laughing]

[@Leekodot15]

(Barely-evil laughing)

[@barsozuguler4744]

I never seen a autoclave before this video. This will be interesting

[@thermomeds]

RIP e.coli. You Will be missed

[@MarcosAlmeidakirito]

"You Will (not) be missed"

[@haekly264]

he was like a father to me

[@Vandarte_translator]

@@haekly264 I (didn't) love him like a son.

[@PauaP]

Not really, I prefee my food to be Ecoli free tbh.

[@sulejmaniapologist]

An hero

[@andricluis]

im more surprised that the blue plastic holder is still normal after heated at 121C for 30 min

[@wildcloud1476]

its heat resistant polymer based plastic. You could drop it from six stories and it would be okay.

[@notyou6674]

@@wildcloud1476 yes but what if you dropped it from 6 stories and it was on FIRE?

[@wildcloud1476]

@@notyou6674 then we would all die

[@PauaP]

@@notyou6674 Big ass explosion would happen.

[@dienosorpo]

Polipropilene is a tough plastic. I sterilize it a lot in my moms pressure cooker lol. It also reaches 121 °C, 15 PSI, and i sometimes do 1:30 hrs of sterilization time. And the plastic resist various sterilization cicles

[@anrie2623]

Autoclave sounds like adventurer fantasy weapon

[@ayzix-polytopia3854]

anrie the AutoCleaver

[@Thecarloful]

This is one of the coolest science channel in youtube, keep it up my friend!

[@trashuser8646]

Kills 99.9% bacteria 0.1%: its time to reproduce myself

[@kamhargrove8694]

Are you talking about the autoclave? Autoclaves kill ALL microorganisms.

[@ramblinevilmushroom]

so.. an autoclave is a fancy pressure cooker?

[@sci-inspi]

Pretty much.

[@urielseptim910]

Mmmm... homemade e-coli in the morning tastes so good from the pressure cooker

[@itsgrace717]

@@urielseptim910 yummy bacteria soup

[@Anthomemes]

There's nothing better than protozoa cereal on Saturday mornings.

[@gessyb936]

One question, is B. Lichenformis infectious? To any organism? Also , lovely music!

[@sci-inspi]

It can infect humans.

[@stap0510]

@@sci-inspi But is an infection harmful to people?

[@sadiev2778]

@@stap0510 Infections are always harmful. Immune response, stealing nutriënts, possibly producing toxcins.

[@sci-inspi]

Yes the infection can harm humans.

[@sci-inspi]

@@FFR2099 I have a bachelors degree in biology. I am a lab tech at a community college. I set-up materials, supplies and equipment for college students in order for them to do experiments in their biology college courses. I also order all the materials and maintain the lab.

[@kirenireves]

Were the tubes covered in the autoclave? How does that work? Also, after the UV test, how do the surviving microbes travel to the dead side? Air currents in the covered dish?

[@sci-inspi]

The cap is vented, it allow air and steam to enter the tube. The microbes don’t travel in the uv test. That is were they were. Just not all of them died.

[@edwchristian123]

E. Coli: didn't survived at many tests Sci- Inspi= *Time to bring out the big guns!* Also E. Coli= *:')*

[@jeremia8515]

Lol

[@frederickboateng3574]

When I saw “Bring out the big guns “ I thought she was gonna bring out Lysol

[@jhoxha]

Autoclave: I have become death, destroyer of worlds .... or petri dish bacterial cultures in our case

[@Jin-di9zv]

Both just deleted. Well, not really surprising for E.coli

[@PauaP]

Well a petri dish becterial cultures can be considered "worlds" if you think about it.

[@jhoxha]

@@PauaP kinda reminds me of that scene in man in black where a whole alien colony lives in jays locker and whenever he opens it they chant Jay Jay Jay

[@PauaP]

@@jhoxha Exactly. So in this sense, the Autoclave literally became death, destroyer of worlds.

[@hilalk-b]

Autoclave is my hero. The first thing I always do in a new lab is to learn how that exact autoclave works or is there a specificly assigned staff to use it and what is its schedule. Even thought I am scared to work in lab when autoclave is on. Sometimes I wish to have it at home.

[@laboonplatoon9263]

Please remind people to autoclave bacteria before disposing of it. I love DIY science but it’s super critical to teach proper sterilization and disposal techniques. Boiling bacteria and culturing it on agar, leading to cultures of a few CFU, could lead to the introduction of a dangerous strain into the environment.

[@Frank-im2ku]

Sorry for my ignorance what does CFU means?

[@jochembraad1760]

@@Frank-im2ku colony forming units. So the few bacteria that survive the harsh condition are able to form many more bacteria and are now a new strain that is more adapted to a harsh condition and thus thus harder to kill

[@Frank-im2ku]

@@jochembraad1760 thank you very much! Now i see why the op was worried about it.

[@hexagonist23]

@@jochembraad1760 1. Take bacteria. 2. Treat with harsh conditions. 3. Take the colonies that survive and treat them with even harsher conditions... 4. Rinse and repeat 5. Create a super-bacteria that wipes out all of humanity

[@olivegarden2137]

I'm glad to have found this channel, not only is the content amazing but the music is just as good.

[@timothykock6799]

This is so fun to watch! I am studying this myself and I'm always very exited to see micro organisms in that way

[@setlenbolstenderods1617]

"I will put bacterial cultures for 30 mins" 2.5 hours later...

[@saint0004]

Great choice of music

[@den0616]

i think he makes them on his own

[@Milkex]

I actually listened to Boiled Dirty Water on Spotify Discovery Weekly while I was building my Openflexure Delta Stage (microscope) without knowing it was you! I had seen the boiled dirty water video maybe two weeks before this and never added 2+2. You just got a sub for life!

[@keyontaeg.6645]

Simply exquisite. My interest in videos like this confirm my own nerdy nature. Thanks, & keep them coming.

[@TreyPDB]

the music is really good

[@jsutien]

*microbes exist* Autoclave: "im about to destroy this man's career"

[@Stogie1]

Autoclave: "ima bout to end this man's whole species"

[@megsley]

my dad does installation and maintenance on hospital autoclaves and other sterilization equipment - he really enjoyed this video! cheers!

[@o0Avalon0o]

The music started and all I could think of was Death Note... Great show.

[@Gvozd111]

What about some antiseptics? Chlorhexidine, ethanol, hard liquor ?

[@johnc7532]

Берзин Григорий ethanol and hard liquor is the same thing. The only difference is that lab ethanol is denatured.

[@Thematic2177]

@@johnc7532 - hard liquors are only about 40% alcohol by volume.

[@easysyndicate]

...

[@sci-inspi]

They can survive a lot of antiseptics.

[@YommyRawr]

@@sci-inspi what about those detergent that claim to kill 99.9% of germs ?

[@hhthoj]

You should make another test. Submerge them in WBC and see if they will survive macrophages.

[@gregoryallen2748]

Your channel is f*king awesome. Seeing these would of made class much more interactive and pertinent to real world than a text book image.

[@lumineaether1494]

ok,this has to be the best channel i have seen right now

[@dal.salvador]

This was really cool and informative.

[@TheDevilockedzombie]

I love these videos. Absolutely interesting and the music is always superb.

[@501joshb]

New to the channel and been watching your videos. I know I’m super late to the party. As a sterile Processing Tech I love seeing Autoclaves get used and the actual results. We sterilize most everything at 132 Celsius for 4 to sometimes 10 minutes for almost all surgical items. Love the vids

[@luciusn21]

Watching your videos in my microbiology class. Love the music!

[@user-fh5ov4tu2j]

The music kind of sounds like Death Note soundtrack, i love it

[@paraumbralin]

You're one cool dude. Also, poor E. coli

[@someonerandom7398]

You will (not) be missed e coli

[@ataplin3058]

Never in my life would I think of an auto-clave as badass until now!

[@mysteriousarcanum2769]

As someone who wants to be a microbiologist, this was very entertaining to watch.

[@aetherblackbolt1301]

If only 2 minutes under a UV lamp did that much, I wonder how good 10 minutes or even 30 minutes would do.

[@isidoraedwards9615]

so cool ☺️ i'm learning about biology on my own through the internet and your channel is awesome for this purpose 💕 is there any way to effectively sterilize objects from spore forming bacteria other than the autoclave?

[@sci-inspi]

Preventing them from growing is best, but they are hard to kill. Autoclaving is the best way to kill spores.

[@isidoraedwards9615]

@@sci-inspi i'll look more into the prevention of their growth thank you! 💗

[@isidoraedwards9615]

​@Unique SP thank you 💗would boiling them for prolonged periods of time be effective as well?

[@capertillar4634]

@@isidoraedwards9615 peepee

[@joesteadman343]

I like the music. At around 00:25 I felt like Kira was going to write my name in the Death Note.

[@unironicdoomer614]

It’s awesome that I now do this kind of lab work at university, trust me guys this is a lot more badass when you yourself are also testing bacteria.

[@RayMak]

This is so much time and effort to make and so educational. Thank you! Didn't know some bacteria can be so tough!!

[@icarokaue7334]

How are you everywhere

[@sams8749]

The beginning of this music sounds like L's theme from Death Note, and I like it.

[@jaxonpontious8018]

I think what you do here is super cool and your channel is very underrated for the neat shit you're doing

[@sheeniebeanie2597]

i just found this channel--this is great!

[@hunterjensen8111]

That one tough microbe! It took a fancy scientific pressure cooker to kill it

[@ec35317]

Great experiments! Thank you! This may be the reason why people who get Clostridium Dificile (which has spores) sometimes get RE-infected since bacterias with spores are hard to kill on surfaces. You used 2 minutes of UV light. How long under UV light would kill the B. Licheniformis completely? Also, you boiled only for 10 minutes. So how long using the boiling method would actually kill the B. Licheniformis completely?

[@extatixz]

As someone who has been battling reoccurring cases of C.Dif for the past year and a half due in part to Ulcerative Colitis, I can confirm. It’s a bitch to get rid of.

[@MyBoyNewton]

Boiling will not kill the spores. They don't metabolize and reproduce but they survive.

[@Brian_Vallejo]

The guitar shredding built up the anticipation!

[@fixitralphltd4988]

Love the music choice when pulling out the big guns for total distruction on all life forms! 🤘😁

[@chevinramheet6858]

What will happen if you boil the test tubes of bacterial culture for 2.5 hours?

[@nerodino5508]

What will happen if you put something really hard to kill (tardigrade?) inside the Autoclave for 25 hours though?

[@trent800]

@@nerodino5508 Tartigrades will be fine if they are in their tun state which can survive up to 150 C

[@haziq880]

Impressive video! Do you usually produce music or the video first?

[@sci-inspi]

I edit the footage together first to see what kind of music I should make. So video and then music.

[@ricksanchez7999]

Wish more channels were like this

[@V77710]

Came for the experiment, stayed for the amazing guitar track

[@cmdrjackwhite5443]

Looks like we all need to start cooking the autoclave way

[@esclapter]

Is the pressure in the autoclave used for actual sterilization purposes, like does high pressure actually harm the microbes by itself? Or is it only used to allow the temperature to go over 100 degrees?

[@sci-inspi]

The pressure allow water solutions to get hotter than 100 degrees Celsius. This will kill microorganisms because some can survive at 100 degrees.

[@Martin_Z555]

awesome demo!

[@ajhproductions2347]

This is all soooo metal...that solo at the end is boss

[@hogane2290]

Your music is so great. This reminds me of Deathnote.

[@jeevacation]

In what way does B. lichenformis affect humans? Nice video btw :)

[@sci-inspi]

It is possible for them to infect humans.

[@headonswivel7569]

Amazing video, that soundtrack was dope also

[@JESUSSavedMefromDeath]

AWESOME! Great Channel!

[@nataliaprzeszowska]

This was very interesting to watch! The microscopic world is being forgotten most of the time by the majority of people although it exists around and within us at all times and we are inseparable from it. I'm learning from your videos, you show great content! Also, I really like your music. It fits perfectly and is well composed. Keep up the good work!

[@lasagna4layf132]

Mother: Doc my son has a bacterial spore infection what do we do. Doctor: We *AUTOCLAVE* him Mother: What does it do? Doctor: It boils and CRUSHES THE BACTERIA AND YOUR SON Mother: Son: Mother: Son: Police: Hi I'm officer John please come with me to the office Doc

[@kami5198]

haha funny KZbin guy

[@akbarputraa7574]

@@kami5198 bro.

[@kami5198]

@@akbarputraa7574 b r o

[@suleiman3417]

i'm in love with the music

[@avenue4624]

Love the music choices

[@wuznab5109]

So all I need to do is jump into an autoclave.

[@jhtang5441]

Who initially thought the Big Guns was 99.99% hand sanitizer?

[@kangmviwgurumindalatrang]

u do it so well. thank you very much sir. great work

[@christianredman2621]

Thank you KZbin recommendations

[@Rhoxe]

E.coli are weak af, God gotta buff them up in the next update

[@Cattus_Maximus]

Then I ain't swimming in any pool.

[@soyderiverdeliverybeaver8941]

Tiermicro

[@ao2415]

Rhoxe .C just main parasites and just farm exp inside a host noobs.

[@tigershark8867]

The Anti-vaxx faction should be an easy target.

[@Sectsoldier]

Nah, let's just vault em until the next patch

[@ninjahunter6925]

I thought it would be like “let’s put different antibiotics in the culture” but you went for the nuke autoclave lol

[@LoFi3]

I’m so glad your music is on Apple Music

[@hanac5586]

This is so very interesting, awesome content

[@TonYnoT117]

Would love to see how effective an ozone generator would be against bacteria and viruses.. video hint ;)

[@aandreoli8961]

No gamma rays :)?

[@tropixMw2]

Vill do same as UV

[@aandreoli8961]

@@tropixMw2 I dont think so, gamma rays are much more penetrating and energetic, they would have the same effect as the autoclave

[@loandx2074]

yeah but where is he supposed to get gamma rays from? 😂

[@nikolaikarpovich1128]

LOL! Love the rock music that played at the autoclave introduction

[@PaceVali]

Amazing videos!

[@armstrong2450]

Music: mass zombie massacre Reality: killing *Bacteria*

[@GygasDistruttore]

Zombie apocalipses are caused usually by bacteria. So it is fitting.

[@halonothing1]

I'm really glad you used an actual UVC lamp instead of just some LEDs or something. I would have lost it if you used LEDs. This was a really great video. I don't usually like it when people just put text on the screen and play music over the video in lieu of an actual narration, but for the most part I found the music was fairly non-invasive. Until the end, of course. :)

[@jhailey2418]

Having online microbiology classes rn, this seems to be really cool

[@michelemond9968]

That was great! More!!!

[@parthgaming1804]

No one: Not a single soul: Sci- inspi: E. coli didn't survive

[@billetede2peso113]

Me: E. Coli the guy she told me to not worry about: B. Licheniformis

[@rabeHH]

Great information. Spores are very hard to kill, for sure.

[@eklectiktoni]

I love your channel!

[@bryanmartinez6600]

Don't worry I got the Hand Sanitizer that kills 100% of germs from area 51.

[@Upgradeo8]

Brad’s Wife would have killed that bacteria first try. 11 years of service dude.

[@sm0lfry198]

As a fan of micro biology, I appreciate this

[@DefaultKnight]

This man is going to fuck around and release a new variant