Four Quadrant Streak procedure - How to properly streak a Petri plate for isolated colonies

[@Ms1101997]

I’m a new microbiology student and I’ll do this for my tomorrow’s lab 😅 I was confused but thanks god I found this video

[@HardyDx]

We love helping new micro enthusiasts! Feel free to browse our other tutorials for your class too!

[@winispecial]

"Thank God you found this video "

[@CrankyRayy]

@@winispecial " " Thank God you found this video " "

[@mdraihan1582]

What's your country name?

[@Ms1101997]

@@mdraihan1582 I’m from the US, California

[@notjeb_9409]

its so wild how a lot of ppl commenting here r college students or professionals n here i am watching this for our lab activity in my high school biology class. cool video, helps a lot

[@clairem290]

I'm a college instructor and I'm sending this video to my students before they do their first-ever cultures!

[@HardyDx]

We love our instructors and our students! Feel free to reach out to us should you need materials for your students as well!

[@Flowers12660]

Well as an instructor why don't you show your students this yourself? Isn't this supposed to be your job to teach? It would be alot better. I really dislike and hate how college so called instructors and lecturers are relying so much on youtube videos instead of doing the work themselves.

[@clairem290]

I did streak some plates myself, which came in handy later. However, a nice KZbin video they could watch the night before was also really helpful. To make up for your previous instructors who didn't explicitly show you enough things, I will now show you how to spell the phrases "so-called" and "a lot."

[@Faceless_Chat]

Very helpful. I have a "unknown bacterium" assignment that starts today. This really helped bc I already forgot you have to sterilize the loop between streaking...

[@daliadivicpuskarski7453]

Yesteday I streaked a Petri dish for the first time in my life. I've watched this video before doing it and it really helped me 🧫 Thank you 😊

[@HardyDx]

That's wonderful. Glad it helped!

[@lorettarodman9117]

I was taught a very different way, always remember that there is more than one way to streak plates everyone! Find what works best for you. Also if you have a bacteria that really sucks at growing on plates from freezer stock I’ve found that not flaming between streaks helps with that

[@sloane.2747]

This was so much fun when I did it, microbiology was my literal favorite class my first semester of college

[@HardyDx]

Glad to hear it! Keep on learning!

[@nina-ge6mn]

I love microbiology and honestly its just so satisfying to watch people streak plate I'm doing a microbiology project that involves e. Coli K-12 plating and im so excited

[@HardyDx]

Nina, we love projects for the tiny humans! Please feel free to reach out to your local sales rep for product, we'd love to help out the next generation of micro-microbiologists!

[@johnson1262]

My universities professor , didn’t tech to put the petri disk , up side down when going into incubator. Thank you for sharing.

[@HardyDx]

Oh no! When media is incubated "upside down" like that, with the media facing upwards, condensation can accumulate on the face of the agar and inhibit proper growth and developing colonies. If you invert your plates, you'll definitely have better-looking plates than the rest of your class!

[@DanTheisen]

This is an excellent video. Thanks, Hardy for showing us how this is properly done. Much more to it that a squiggle of bugs on a petri plate!

[@HardyDx]

Glad it helped!

[@DanTheisen]

As required by law, I’m disclosing that I was employed by Hardy Diagnostics at the time of this comment.

[@Sean-ur4cb]

@@DanTheisen what did you sign that told you to disclose this info?

[@DanTheisen]

I don’t recall where I saw it but this is, as far as I know, meant to prevent large companies from having their employees promote their products as if they are customers. I have not signed anything from the company compelling either a comment or a disclaimer about the comment. The law as I read it made sense to me from a consumer protection point of view, which is why I happily added it.

[@Sean-ur4cb]

@@DanTheisen ahh I see, thanks for the reply. Have a good day!

[@Cyan-J-e5u]

It's self explanatory and the visuals, helps for easy remembrance. Thanks.

[@HardyDx]

You are very welcome. I am glad our video was useful for you.

[@mr.crimson5109]

3:16 i have an idea for faster cool down. you can use normal saline solution for cooling it down, must be done aseptically.

[@AL_ayyubi1137]

I'm medical laboratory technician(MLT) and 11 lecture is included for simple technique in tomorrow thank you for this video I appreciate it 🤩

[@HardyDx]

You are welcome, I am glad the video was helpful. Keep up the great work as a MLT. You are important.

[@Dananq98]

So useful description, thanks a lot 👏🏻

[@HardyDx]

Glad it was helpful!

[@ayesha-it5yw]

I learnt this method today in my microbiology lab.

[@HardyDx]

That's great Ayesha! Keep up the proper four quadrant streak method, you will be a pro in no time!

[@leannajulien4175]

I didn't know I have place it in the heat everything I learned something new. Thanks

[@HardyDx]

We're always here to help you learn! We have a great Technical Services team should you ever have questions!

[@ry_n007]

thank you for saving my ee broski 🙏🙏

[@tayyabirshad3029]

Today may microbiology practical exam it's help alot!!🥰🥰🥰🥰

[@HardyDx]

Glad to hear the video was useful. You are very welcome. Good luck with your exam. You will do great!

[@anycamenzind]

Will there be a video available that talks about the microscope and Khoeler illumination? Unfortunately, part of the practical microbiology and laboratory sessions were taken in times of pandemic. Likewise, I would greatly appreciate if you could explain how the UFC colony counter works. Your explanations are great! Thank you 🙌🙌🙌

[@Manthab]

Don’t know how I got here at 1am but it was fascinating and informative if I ever need to know this I’m covered😁

[@HardyDx]

Welcome down the rabbit hole of microbiology, Mantha! We've got a whole channel of videos if you ever need more obscure information to get you even more hooked 😃!

[@nangsanbhalangblah333]

Great vid, I've a question though. Why is the media red in color? Is there a different type of agar used?

[@918mounika7]

It's probably blood agar medium, blood added to regular nutrient agar medium

[@HardyDx]

Nangsanbhalang Blah, Great question about the coloration of the agar! This was streaked on our Blood Agar, an all-purpose growth agar with hemolytic properties. Some organisms will hemolyze the blood in the agar, leaving clear zones around the colonies, something we won't see on traditional TSA. Hope that helps!

[@AliyuNanaaishat]

Please can I see a video that will teach me more on how to carry out indoor air quality using SDA and PDA

[@JoshAngelo-k7b]

How do you report colony growth on a 4 way streaking? along with its size, shape, transparency, consistency, and color.. Thanks

[@ruchika_899]

Gorgeous explanation 💥❤️

[@HardyDx]

Thank you, glad you liked it!

[@apokalypthoapokalypsys9573]

I'm in an IT MSc but this is perfect ASMR

[@HardyDx]

Love it! That's a whole new industry we should break into!

[@JesusLightsYourPath]

In my class they never told us to reheat the probe before creating the next spread section. Is that why our colonies are always too big? Or is it possible to make small colonies without burning the probe in between each swab?

[@HardyDx]

@JesusLightsYourPath Great question. The reason that the metal loop is sterilized for each quadrant is to eliminate extraneous 'crossover' of organism when you streak each quadrant out. By the final streak, the number of cells on the loop should be diluted and low enough that single colonies are dispersed and isolated on the agar surface. If you were to not sterilize between streaks you would end up with a much more confluent 'lawn' of bacteria because of all the extra organism on the loop when you go to streak your successive quadrants. To learn more about how to properly streak a Petri plate visit our blog. hardydiagnostics.com/blog/petri-plate-2023

[@KHALILLABSKILLS]

It's absolutely Amazing sir.

[@HardyDx]

Thanks, it's our pleasure.

[@jessical4866]

I’m not a teacher or a student… why am I so fascinated with this? 😂

[@HardyDx]

I mean we can't blame you, we do this for a living. Our whole lives are this, welcome to the obsession!

[@GaneshKunayak]

Who are you 🤣😂

[@affiliatemarketinginnigeri7617]

I'm a passer-by as well and I am entranced ❤️

[@ruchika_899]

Excellent explanation ❤

[@HardyDx]

I am glad our video was useful for to you.

[@KHALILLABSKILLS]

I really appreciate you sir.

[@jennisagala1263]

thanks a lot, i hope, my thesis will be finisih

[@1alex92]

Wish I seen this 2 weeks ago when I had a test! ...nevertheless.. I just subscribed ☺️

[@HardyDx]

Welcome to the online Hardy family! Stay tuned for more great content soon to come!

[@vennelagovindugari5420]

Perfect!!

[@NahalFatima-x7j]

I did not understand one thing...can anbody answered? Do i have to heat the heat the loop every time before streaking?

[@nithinmuluka7987]

Thank you sir very nice video... Pls upload more videos

[@HardyDx]

Yes, stay tuned. More video are on the way!

[@todddillerson4053]

I do this every day for the hospital I work at :)

[@HardyDx]

That's great! Heroes wear lab coats not capes. Which hospital do you work at?

[@Hellamoody]

@@HardyDx boundaries Dwight!

[@joannadevung4358]

If i accidentally tore the surface of the agar using the loop during streaking, will the bacteria colonies still able to grow?

[@HardyDx]

Colonies will still grow, yes. However, it is usually best to restart on a fresh plate as the puncture could lead to difficulties in reading/counting colonies or could cause unwanted areas of heavy growth. Don't worry though! Marring the surface is extremely common, especially in beginners. Keep practicing and you'll be able to do it as if it were nothing!

[@mohanzm12]

What do we call the thing she used to flame the loop ? 😅

[@DadaClaire]

I'm curious too. I'm working with unicellular fungi and bacteria since time, streaking them to isolate but it's the first time ever I'm seeing that instrument.

[@vanessadang4674]

It is called a bacticinerator :)

[@DadaClaire]

@@vanessadang4674 Thank you!

[@HardyDx]

@Mohan The tube the loop was inserted into is a special type of sterilizer. Placing non-sterile metal loops into this tube for a few seconds will sterilize these loops so you can re-use these again and again. Hope that answers your questions!

[@mohanzm12]

@@vanessadang4674 wow .thank you

[@YusmaArfanDogar]

I'm student of DPT and tomorrow I have to perform this practical

[@KHALILLABSKILLS]

It's well explained.wow

[@abdullahmohammad6803]

I did it last week, applied so much force that it tore the agar . I’m worried I might do bad for the practical.

[@sunny4483]

Can we not streak circular on plate instead of streaking zig zag? I'm curious

[@MrDubbindan]

There is an instrument that does streak with a plastic "comb" after pipetting a volume on a plate and it does make circles ... The Previ Isola

[@Szaisthatyou]

I love my major ❤

[@RaiSaysS]

Thank you

[@HardyDx]

You're welcome.

[@SaidaZaknoune]

Quand utiliser la methode en stries?

[@ceciliaovansa2738]

Nice one

[@HardyDx]

Thanks for watching

[@hebahariri9936]

Hello, I'm a microbiologist and a content creator on Instagram educating microbiologists in Arabic, can I translate your video and post it on my account? with sharing your video link of course. thank you.

[@HardyDx]

Yes, that would be fine as long you tag and credit Hardy Diagnostics in your video description and link it back to the original video. Keep up the great work in microbiology.

[@GaneshKunayak]

Bdw i think this must be carried out inside a laminar air flow

[@LìXià-o2q]

Isn't it 60° degree ?

[@pastmonster446]

Watching this after I took my lab practical exam, and I definitely fucked it up

[@akritikuikel8506]

how to get a job or internship of microbiology in europe. I have done bachelor in microbiology and working in a restaurant since 4 years. Did not even get any inernship. Already forgot all the techniques and skills in these 4 years . sad sad

[@BlueFox61]

Build a portfolio of your interests and maybe your thesis paper too. It's hard to get an internship now

[@M313_1]

*ثانكيو* 🦦

[@chrisk6469]

Watching them tear up the agar like that was a bit painful ngl

[@lilianmumbo880]

5 method of preparing specimen

[@SOPHIA-yf7ws]

A++++

[@mtado4933]

yeah i fucked it up so badly

[@عبدالرحمنحسن-و6ث]

عربي وين

[@Behejrbrbrjrj]

هنا موجودين 🙂🙂

[@kasisfile]

😮

[@alvinxyz7419]

I thought the title said steak

[@sriningsih4808]

kangen haha

[@John-fr6wo]

For God so loved the world that He gave His one & only Son, that whoever believes in him shall not perish but have eternal life. For God did not send His Son into the world to condemn the world, But to save the world through Him. (John 3:16-17)✝️

[@brystopczynski1295]

Wha??? She's literally doing like 10-20 passes AS THE NARRATOR IS SAYING "no more than 2-3" or "1-2"!? 🫴🫴?! Is her technique fine? Sure. Is it the best? No. But what a terrible example 😠 at least be consistent when making an instructional video.

[@HardyDx]

@brystopczynski1295 Thank you for your comment. Yes, this is the correct technique used by microbiologist. When streaking a plate the goal is to reduce the number of cells to isolated colonies from quadrant to quadrant. So when the narrator says overlap no more than 2-3 times, it means only overlap from quadrant #1 to quadrant #2, 2-3 times, then continue to streak the remainder of the quadrant. Each time you go to a new quadrant only overlap your streak 2-3 times, then finish out the streak with the 10 -20 passes shown in the video. Your feedback is heard, and we will continue to improve our videos. We are committed to providing a Culture of Service. If you have any other further questions don't hesitate to reach out. Additional if you would like to know more about the four quadrant streak feel free to visit our blog to learn more about the process. hardydiagnostics.com/blog/petri-plate-2023

[@FarzadSalim]

thank you

[@HardyDx]

You're welcome.