What is a gap in

This video explains with examples about how gaps are introduced into two aligned sequences in BLAST and how using a formula we calculate gap score and max score.
My other videos on BLAST-n and BLAST-p demonstrate step by step how to use BLAST for nucleotide and protein alignments and how max and total scores are calculated.Here are the links-
• UNDERSTAND, LEARN AND ...
• Understand protein-BLA...

[@NeelamnishanthreddyReddy]

in your example first gap score does not contain any extension 5+2*0=5

[@biosolutionsbygunjan7474]

since there is one gap only, the value of "n" is 1. n can not be zero.