How To Grow Mushrooms | Doing Agar Work With Mushroom Cultures

[@kerem7546]

i've found that by having my lab bench a little bit higher it helps take some of the strain off my back when working, would highly recommend it!

[@leekeebum9406]

Brilliant detailed video, good to show in detail how to transfer plates, and save strains from contamination. It's the little tings that count, 3 cheers for Brian

[@markcarney9144]

I cool off my scalpel with a quick dip in a small glass that has 70% alcohol in it. Also, when saving mycelium from a contam'd dish I do that work inside a still air box rather than in front of a fan so I'm not blowing spores all over myself and the room.

[@COMEGETSOME27]

Thanks for sharing this Brian, great video!! Very helpful as a beginner looking into starting a little mushroom library and looking to learn about the processes as a whole and what goes into keeping it sustainable! Really appreciated the detailed content, awesome energy and inspiration so thank you brotha! :)

[@dirtdiggerjerry]

I'm just getting started. Thanks for the advice.

[@DCupsHerbs]

Thanks for all the beautiful and useful information. Watched a couple of your videos now and just starting up my mycelium hobby / hopefully business in the future greetings from the Netherlands

[@rendacomcogumelos]

Hi, thank you for the video. I have a question about the filter. The filter you are using is a Hepa H13 or H14? what the velocity of the air? The consulter where I am trying to buy a HEPA h14 said that is necessary an air velocity of 0.45-0.8 m/s.

[@Trimdeluxe]

Once the transfer is done, how do you store the plates while mycelium is growing? Also on average, what is the growth rate of mycelium on agar?

[@AreaHQx]

The thing i like most about having plates on hand (my case jars) .. The condensation built up mostly evaporates by the time you use it...

[@future3325]

i saw the loop sterilizer and knew i was in for a treat. edit: you need a tube rack

[@bryanadz]

Hi, very nice video, can you explain if it's possible to transport some mycelium from other countries (UE- USA ) to nord Africa, I want to make a small mushroom farm using mycelium of lion's man ...etc witch are not known in my country, do they have a lifespan ? And if are capable of being transferred? Thanks and best regards

[@davidcolle7335]

Thanks for sharing, super informative! Lab skills and process are really important and theres not a whole lot out there to help with the learning curve. very timely for me as I have been spending alot of time in the lab lately refining my process, finally working on backing up my cultures. Funny note to leave you with....I was pouring plates the other day and wouldn't ya know it the mason jar I was using slips out my hand and of course it pours hot agar on/into my flow hood, so the lesson that day...get a handle of things. Simple yet so valuable.

[@whatthefungus]

oh man, that sounds like a nightmare. I once dropped a glass jar of spawn on my lab floor when it was in my spare bedroom back in 2013, and had to remove all the shelves and sweep up the mess. It was a lot of work and frustrating.

[@joshsmushroomsandfarmlifeo6178]

Where did you get the electric sterilizer for your knife?

[@josephnorris1007]

Thank you So kindly for sharing you work. Each piece so valuable. Quite the Gift

[@kennyofatoboiyo7447]

Hey man just curious I thought it was bad practice to so agar transfers where one is infected with mold in front of a laminar flow hood because the air from the flowhood will cause the mold spores to blow all over the room in which you did the transfer leading to even more contamination. I thought in this instance you would be better suited to use a SAB?

[@lukehalpin8571]

I'm starting up a mushroom farm in Portugal on zero budget, I think I already died but I will respawn somehow. I got to find a way to get good substrate, the wood pellets are mainly softwood out here. Thanks for your videos they are very helpful

[@hhyy3173]

What a great lab! Nice work!❤️

[@HopeSlayer954]

What is the brand of your sterilizer for the knife?

[@yangchen7982]

Hope Slayer on amazon. Look up “infrared inoculation sterilizer”. They’re only like 200 to 400 dollars lol

[@noelmansfield8505]

What temperature do you keep your room?

[@Noe18091]

good videos I see them from colombia south america and I want to put the translation option because I am very interested in what you say, I also have a mycelium laboratory in my house and I have not been able to get even a petri dish with mycelium mother to inoculate grain . the boxes with culture medium always begin to sweat that is normal? with or without inoculum sweat I do not know why I cover them with parafilm and that always happens to them, that I will be doing badly.

[@awesum2230]

I am not going to say this wasn't a very informative video. I do a ton of agar work myself and one of the number one rules is NEVER open a contaminated dish in front of a flow Hood. I'm sure you can understand the science behind it. The mold spores that are in there will begin to flow around everywhere. Still air box work for contaminants only. At least that's my company's rule of thumb. Please don't think I am trying to BASH you. Just some constructive feedback. But I'm sure you know enough to know what you need to know. Great video!

[@YouOriginal]

^^^^^^

[@stenny4days389]

Is there an update video on the contaminated transfer? Did it work out? Did you need to transfer again? For really bad infections how many transfers do you think it could take to isolate? Also, I have a microscope. How can that be of use in these processes? Like someone else said, it’s hard to find good videos. Maybe I should look at microbiology vids

[@timburleigh9661]

Please where can I find one of those scalpel sterilizers ?

[@yangchen7982]

Tim Burleigh on amazon. Look up “infrared inoculation sterilizer”. They’re only like 200 to 400 dollars lol

[@rompstar]

those round plastic containers you use to start off your ML (not the test tubes or agar plates), what are those ?

[@whatthefungus]

32 oz PP jars from US Plastics

[@rompstar]

@@whatthefungushow do you sterilize them, since they are plastic ?

[@rompstar]

@@whatthefungus how do you sterilize them given they are plastic ?

[@lololollaughatlife1431]

How do you make the agar in the Petri dish? How do you collect the specimens to use in the first place?

[@semperviren674]

How do you dispose of the contaminated dish?

[@yangchen7982]

What is the temperature in the room where you store your plates?

[@valleyshroom7572]

What is that little stainless steel rack called that's in front of your flow

[@TR-gg5nt]

HEPA filter

[@crucialmatt]

What is the real name of that heat tool you stick the needle into yo get it hot?

[@yangchen7982]

on amazon. Look up “infrared inoculation sterilizer”. They’re only like 200 to 400 dollars lol

[@jamessharp7330]

New to agar work. Thanks for your patience. Where are the vents on the petri dishes you recommend, honestly I am a bit confused about the concepts of vents in petri dishes in general? I know they need to breathe but aren't the lids on them already loose? I didn't notice you covering any vents with tape. Do the boxes of 500 come in sleeves? Are they sterile?

[@johngrady1755]

Another great video. Curious about where you buy the plastic jars you use? Looks like you put on the filter disks yourself. There's always something satisfying about the time lapses, and you always use a certain type of music that I've come to associate with WTF. Funky jazz reminds me of Let's Grow Mushrooms and It's neat to see the different styles reflect different personalities.

[@whatthefungus]

Jars are from US Plastics, filter patches from Fungi Perfecti. Love the jazz timelapse !!

[@robslams2324]

when i pour agar infront of a flow hood, do i need to leave the cabinet running until it solidifies and i can wrap it with parafilm? im used to a sab but i just spent a grand on a nice hood, but i dont know about when the agar is still warm and liquid and you just poured it.

[@ryanbarrett1949]

Thank you ! Great video once again!

[@margeauxeatsushi]

What do you think about T.R.Davis's recent video about making your own spawn (or actually NOT making your own spawn)?

[@brettlatulip67]

Jees I wish I found mushrooms in my 20s instead of late 40s. Still... it will be done! Mush on! I would have watched even in slow mo🤓

[@JAleksandr]

43... right there with ya!!!

[@iknowyourerightbut9392]

How old is your oldest daughter Brett?

[@stochastic24]

I'm 39, glad I got into mushrooms at such a young age :D

[@bossanovabossanova3386]

wait so thats just sawdust? you dont need the extra stuff that a kit comes with?

[@joaovalentim945]

How do you store your test tubes? And how long they last? Just love seeing your work!

[@sedimalko]

Hello. Very interesting cinnamon and quite instructive! I have a few questions. Would you share your experience with us? 1. Have you tried putting agar or wheat mycelium in a chamber. minus degrees in order to stay longer. What are the results? 2. Can you move from agar to agar after a certain period of time. As far as I understand, there are up to about 4 times the grain after which the qualities are lost. For example, after 2 or 3 months, move to a new agar? 3. How long can the mycelium persist and what are the steps? Thanks in advance!

[@louispconstant6624]

Did you ever find out the answer to these questions at all pal?

[@louispconstant6624]

Just starting for the first time to work with agar using spore prints and trying cloning subs

[@sedimalko]

@@louispconstant6624 Yes. I found the answer to all my questions! It took a long time but I found them! Thanks for the reply! Get healthy!

[@dambition7495]

How long can a colonized plate last for storrage?

[@mimimajgaard9651]

Where can I get the flow hood from? And are they all over $1k?

[@roontunes]

wher doyou get the test tubeculture. thanks for poosing this

[@alexandm1001]

curious if over spray from the alcohol can kill the mycelium if it lands on the plate

[@whatthefungus]

Yes it can. 3% peroxide will not kill mycelium however

[@awesum2230]

although it will kill spores! So no peroxide with Spore work. Only with mycelium work. There are actually agar recipes that contain peroxide :-) and it is very effective I might add from personal experience

[@garfoni]

Hi, for how long can you keep the test tubes without getting bad? And you freeze them for long term storage? Thank you!

[@BrettMerrow]

This is awesome thank you

[@fighttilltheendfighting6819]

how about spore print to agar?

[@leehuntley189]

Are these nalgene containers? Or just pp

[@vitaly5209]

Hi, man! I followed your chanel for a long time and i think you experienced enough to know the answer to my question. Why should we do tissue culture? Can i Just take inoculated and fully colonized grain to inoculated another sterilized grain to get new mycelium. I found in Internet that agar mother culture needed to make stronger generation.Quote:" The strength of mushroom culture is maintained by preparing mother culture. The culture become weak when subcultured several times." How do you think?

[@Farmhousehorror]

What kind of vent hood are those

[@jeanwhite5913]

This is off topic but how do you figure out what the market is when there is no market where I live?

[@oforihubert2192]

Thank you, excellent, I want you to teach me how to do it.

[@tomaapple3573]

Question I’m getting into mushroom farming I live in the USA where can I get good strains? Or should I buy a live mushroom locally and clone the mushrooms.

[@whatthefungus]

You can get good strains from me

[@tomaapple3573]

WHAT THE FUNGUS cool thanks I’m looking at what the fungus website but I can’t find any strains that you are selling

[@whatthefungus]

just send me an email. I have not designed a tab on my website yet for strains. [e-mail]

[@averagemo3tx960]

Are these psilocybin/ psychedelic mushrooms??

[@Wickedstickyflowers]

can you go from syringe to dish

[@seussiii]

Lookin good! Might be one of my orders

[@whatthefungus]

You know it.. just waiting on more plates to complete the order.

[@seussiii]

No worries, brother! Got plenty to keep me busy!

[@ranacaran]

What about all plastic bags? Are they one time use?

[@whatthefungus]

Yes they are, however I am currently working with Unicorn Bags to develop a Bio-degradable bag option

[@ranacaran]

@@whatthefungus great that would be awesome...check this out kzbin.info/www/bejne/oJ-wiXtridakfZo fungus packaging

[@xxpowwowbluexx]

.

[@robertedwards9809]

Good lord! I'd never open a Trich contaminated dish in my lab! That could easily prove to be the mistake that keeps on giving.

[@whatthefungus]

naw its fine

[@robertedwards9809]

@@whatthefungus I get that the filter hood will handle the threat just fine. But still feel like that hood just spread green death over everything down flow of the hood. Might be an interesting experiment with some open plates behind a contaminated plate behind a hood, see what comes of it.

[@robertedwards9809]

@@whatthefungus but your gonna have to be the one who conducts that experiment, because even in the name of science I refuse to open a contaminated dish inside my lab,. Hahah. Great videos man! Excited everytime a new one comes out.

[@RunescapeGod]

Didn’t look like trich to me

[@johnnyt7900]

Cool dude

[@themyceliumnetwork]

15:56 do not try this at home. actively sporulating mold should not be opened in your lab environment !!

[@andreslorenzosantana4920]

hey brian, there is a special lab sharpie that you could use to write on wet surfaces and doesn't smear with water or alcohol (useful for petris, slants and bags) www.fishersci.com/shop/products/fisherbrand-fine-tip-marking-pens-4/p-158236

[@whatthefungus]

thanks but I like milwaukee markers..half the price and great quality www.blackrocktools.com/milwaukee-inkzall-black-fine-point-marker-4-pkg-48-22-3104.html?gclid=Cj0KCQiAg_HhBRDNARIsAGHLV523l9RSsnTnE25mLhhg12M9dw31d9svTYU_Y5kUojLwGytgby9xjYUaAhOXEALw_wcB&s_kwcid=AL!3645!3!304422442490!!!g!!&ef_id=W-2g2wAAAK6kLyqd:20190114193041:s

[@andreslorenzosantana4920]

I said it because yours is deleted with iso, and the lab marker saves time by not having to rewrite the petri. Anyway, thanks for the video!

[@Lisa-mk7dt]

TARA LEE shared this with is intersting stuff , I'll be back. 🌱♳✌🏼

[@dingdongshlong]

Use plant parafilm tape instead of expensive parafilm and save money. Trust me

[@gustavohenao9586]

Good work and thanks. Suggestion, if I may. Write a script so you won't be speaking more than you need and particularly you won't repeat over and over that you are going to save the penicillium contaminated box. But, thanks for the good info. Good look!

[@jcjensenllc]

I would rather get process, protocol details versus an inventory of your cultures. NTK: temp, humidity, duration , light level, transfer techniques, how to spot contaminants, sources for tools, supplies, costs, sources of inoculants, etc.

[@jcjensenllc]

In my readings about soil health everyone talks about how fungi works symbiotically with other organisms. You patureize your growing medium to kill everything much like practicing monoculture in farming - not healthy and reduces nutrient density in crops. Please comment.

[@xxpowwowbluexx]

.

[@jcjensenllc]

FYI, Your skin is home to protective bacteria, part of your immune system. Isn't there a better way to prevent contamination than wiping your skin with alcohol?

[@geinstein8407]

Dude 87 dollars for 500 dishes are not a good price that's almost more then I sell them fro well making a profit Pity if you want I'm in South Africa but I'm shore the shipping cost will then make it expensive

[@tomchrissuicagoins1900]

You're not GONNA DO IT!!! OMG you did.... ru crazy? Dude If you have a 10 mile by 20 mile flow hood, open moldy plates inside a SAB thats why thay teach SAB to noobs first. You went straight to pro and skipped the noob part.

[@Mueneeez]

Ego maniac