How to Make Mushroom Culture Slants for Long Term Storage

[@abnervalbuena9774]

Excellent..!!! This is the way knowledge should be given. Direct and simple. Thanks

[@SporenSprout]

Thank you! 🙏

[@Allyourbase1990]

I’ve always wondered why ppl use these over plates . This is pretty cool . May have to try it out !

[@ScottWConvid19]

There are only a few mycology channels that I find to be the most informative with practical demonstrations for the hobbyist. Yours is one. Thank you for making the time to think these through and share the details

[@chicofromph33nix64]

Along with Philly golden teacher and 90 second mycology

[@ScottWConvid19]

@@chicofromph33nix64 I have not found them to be so. They are mostly focused on recreational psychoactives

[@naythansouthafrica632]

You answered questions i had that i could not find in other videos . I like that you mention every part of the process including mentioning incubation , parafilm , lifespan etc . Well done and thank you

[@SporenSprout]

Thank you for watching and for the feedback! Glad it was helpful!

[@DevoutJourney]

Is it best practice to go from an agar plate to slant instead of fruit body sample to slant, to identify contaminants?

[@SporenSprout]

Yes

[@thegreenroomorganic]

Thank you for this! It’s a game changer. My question is : so I where I live I don’t have access to a lot of things. So for my agar recipe I use boiled potato water with agar. Do you think it’s alright to boil the potato and the pop sticks together and mix agar into to that medium?

[@SporenSprout]

I think that would work fine!

[@michaelcarey9359]

Retired Semi-Pro Tip: For LOOONG term storage, a little mineral oil over the slant's colonized surface will prevent the culture from drying out.

[@zainuddin3680]

I guess you should sterilise the mineral oil too?

[@adamschaefer423]

Why use the water you boiled the sticks in and not use fresh water instead?

[@SporenSprout]

Just to save time, doesn’t really matter.

[@orionsbelt3827]

Would you use the premade agar/supplement powder that you showed in your agar petri dish video for slants?

[@SporenSprout]

Yeah that works good

[@skyelord6229]

Excellent video🍄

[@SporenSprout]

Thanks for watching! 🙏

[@thedude8122]

What is the purpose for adding the popsicle sticks?

[@SporenSprout]

Basically the mycelium permeates the stick and is able to survive for longer than the mycelium on the agar surface. It provides a protective barrier from outside elements and disturbance. Its like a fail safe for your culture in case the agar dries up you can pull the colonized stick out many years later and revive the mycelium.

[@thedude8122]

@@SporenSprout Very cool, Thanks for the response.

[@orionsbelt3827]

@@SporenSproutfascinating. Where did you learn about mycology? Do you recommend any books?

[@kosmika_didjeridoo]

It's better inoculate with agar or with liquid colture? Thank you

[@SporenSprout]

@@kosmika_didjeridoo Agar is better

[@TheShroomate]

Why didn't you mention what the popsicle sticks are used for?

[@SporenSprout]

@@TheShroomate They are like a last resort for the culture. If years later it looks dead on the surface. You can pull the stick out and put it on agar and it should grow.

[@ZeStaticWolf]

Why do people put popsicle sticks in there slants???

[@SporenSprout]

The mycelium will colonize the stick and if the mycelium on the surface dries up over a long period of time you will still be able to pull the stick out and put it on an agar plate to revive it.

[@TheScientist777]

Thanks for the LC!!!!

[@hebaaly1382]

What is the type of malt extract used? Is it from local stores or laboratory grade?

[@SporenSprout]

Light malt extract from local brewery supply store or online here: amzn.to/4bB7Ict

[@coloradodirtbike5930]

You prefer the 50 ml slant over the 15 ml? I see you use both, you notice any difference between the two sizes

[@datasoftmanagers9409]

What is the refrigerator temperature?

[@SporenSprout]

40 F

[@Its_me12759]

Thank you 😊

[@SporenSprout]

Thanks for watching!

[@hansdampf2284]

What is the popsicle stick for?

[@giuek8389]

Question: sorry if I missed the part but what temperature do you recommend for the refrigerator / long term storage? Great video👌

[@SporenSprout]

39 degrees F is good.

[@paulofx408]

​@@SporenSproutwithout fridge how much time a slant would last? (room temperature)

[@szilardhertelendi7417]

​@@paulofx408 I have slants 1 year old and still living, but best scenario is storing them in refridgerator. Lifetime can vary per specie and per strain. And also might require some transfers to get them back to their strength. (They were living in oxigen deficit for a long time)

[@erniebusby9180]

What brand/model is your flow hood or did you build it yourself?

[@SporenSprout]

@@erniebusby9180 it’s a Camfil FFU I picked up at a clean room supply store in my state. It was $1000 and I didn’t have to pay for shipping

[@ourfamilyoutdoors7331]

Why can’t I just load the tubes into the PC?

[@milepetricevic7267]

How long can this slant stay sterile before inoculating

[@SporenSprout]

12 months or longer

[@glennplatvoet7111]

Thanks it was informative

[@SporenSprout]

Thanks for watching! 🍄

[@marlibrown5174]

whats the name of the tool used, to cut the wedges

[@SporenSprout]

Sterilized disposable scalpel is good to use.

[@henryLA310smokes]

@@SporenSprout your doesn't look like the typical sterilized scalpel. It looks long with a squared off end you used to cut wedges. What is that tool or called to look up online?

[@SporenSprout]

@@henryLA310smokes amzn.to/4e8CDhk

[@henryLA310smokes]

@@SporenSprout thank you!! Ordering now :)

[@stayanddrown]

My slants warped or became concave in the middle. Any clue why that would happen?

[@MB-sn8sf]

Didn’t loosen the caps enough to let gas escape maybe?

[@Eric-yj5xg]

What molecule is your tattoo? Also great video!

[@DevoutJourney]

Hey brotha, so I made slants today for my gen1 LM, but I forgot the damn popsicle sticks!!! How viable are these gonna be for long term storage? They are the same tubes you got.

[@SporenSprout]

They should still be viable after one year. I would transfer it to another fresh slant next year.

[@DevoutJourney]

@@SporenSprout thank you sir! I just watched your video on the top 4 medicinal mushrooms and I think I want to try growing them. I’m gonna try my hand at a double extraction tincture w some of the lions mane I grew this weekend. Well, at least get it started.

[@SporenSprout]

@@DevoutJourney Thanks for watching that video! 🙏 Congratulations on your successful lions mane grow! That’s awesome 👏 🍄. Dual extracts are the best way to go, good luck!

[@DevoutJourney]

@@SporenSprout hey brotha, how can I store my lions mane after harvesting. At least for a few days without them turning brown or getting fucked up? I’ve got this second flush wrapped loosely in parchment paper, and in a bowl on the counter

[@DevoutJourney]

@@SporenSprout hey man so it’s been I think 6 days and my agar slants aren’t growing any mycelium. I took tissue samples and have em in the proper environment. Did I do something wrong?

[@orionsbelt3827]

Hey dude! Sorry to no blasting your comment section but I need that tool you use to transfer agar wedges. What’s it called?

[@henryLA310smokes]

yes! @SporenSprout, we need to know! lol

[@MadScientist816]

Hey brother hope you are well Badass content! Mad luv Peace out Mad scientist organic grower✌️

[@SporenSprout]

Mush love brother

[@MadScientist816]

@@SporenSprout ahahaha well said, keep it up. Mad luv

[@SmokinAce7]

What’s the reason for the popsicle sticks?

[@SporenSprout]

@@SmokinAce7 It’s like a backup plan for long term storage. The mycelium will eventually colonize the stick deep in the agar, protecting it better than the surface. If something happens to the surface mycelium you can pull the stick out and put it on agar to regenerate the mycelium.

[@WhiteWolf1980]

​@@SporenSprout yup. That response just got you a sub. Thank you.

[@SporenSprout]

@@WhiteWolf1980 Thank you! 🙏 Glad to have you.