Your Home Yeast Lab Made Easy - Aseptic Techniques

[@tube4waldek]

Amazing lessons all over your channel! Thanks!

[@gabrielfusaru1211]

I would seeking for some easy understandable lessons about yeast growing and I found your lessons. thanks!

[@SuiGenerisBrewing]

Some on commented, then deleted "shouldn't aseptic techniques include gloves?". Not sure why they deleted the question (its a good one), so I'll reply to it anyways. In the micro lab we always wear gloves if working with pathogens, and never wear gloves when working with non-pathogens. The reason is a balance of risk and benifits. Gloves provide minimal protection for your samples, as only dander/etc from your hands is blocked - stuff in the air, falling from your face/hair/etc are not impacted. So there is a negligible advantage in terms of protecting your sample. Gloves do provide a lot of advantage in protecting you, as hand contact is the most common source of self-contamination when working with microorganisms. But there are costs to wearing gloves - you lose dexterity, and if you pass the gloves too close to the flame they can melt to your hand or even catch fire, causing a serious burn. So you have to balance the risk of wearing gloves to the advantages they offer. For a brewer, the advantages are far outweighed by the potential risks; in other microbiology circumstances the risk:benifit ratio is different.

[@HaikuAutomation]

0:03 Introduction, Aseptic Techniques. 0:18 Most important thing, clean and uncluttered workstation 0:38 Clean yourself up, wash hands, tips and tricks. 0:58 PSA on hydrating soap, vs antibacterial soaps. 1:14 Start working on your work area, and additional clean room techs. 1:35 Gloves, why, when and how to use them. 2:16 Working properly with your burner/lamp. Slow movements, spacing, etc. 3:33 Most important tools, the loop (and others) 4:42 Cooling loop to agar, or dish/cup of 70% alcohol. 5:47 Flame and working with tubes, keeping tube clean and sterile. 6:38 Flaming the tube, cap, whatever through flame. 7:04 Working with plates 7:42 Flipping gently a plate/dish 8:14 Working with plate with loop 9:10 Thanks for watching.

[@randomness3235]

Wow, this very useful and great information! At least for my needs... Thank you so very much for providing this knowledge, it will certainly be of use and help.

[@SuiGenerisBrewing]

Glad you found it useful!

[@jasonclick]

excellent video. thanks for sharing.

[@SuiGenerisBrewing]

Thanx!

[@nortonshields8405]

great video - can you make additional agar slants and store them until you have the yeast to culture them?

[@SuiGenerisBrewing]

They only last about 3 months in the fridge, so not really, unless you're expecting to do a lot with then short-term.

[@kartalbaba981]

Thanks for sharing for that great video.

[@SuiGenerisBrewing]

Glad you liked it

[@b.e.d.brewing3909]

Very informative, also don’t some gloves have a residual powder on them? Seems like that would also cause problems

[@SuiGenerisBrewing]

Powders on the inside.

[@lacaval]

Will using 2 or more alcohol lamps aid the updrift and make the workspace bigger?

[@SuiGenerisBrewing]

I doubt it. Using a lamp limits your workspace - the area behind is essentially unusable as it is difficult to access materials there safely (i.e. without burning yourself). While more lamps would give a larger updraft, it would also make for a larger danger zone. Unless you are working with particularly large plates or flasks, a single lamp should be more than enough.

[@Ray.5446.]

How would you inoculate flour for bread making?

[@SuiGenerisBrewing]

Suspend it (either dough, or dried yeast) in some sterile water, then spread it on the plate.

[@TrevorFisher]

Is there any scenario where you would not advocate for quenching in alcohol and flaming off the loop? (Other than it's a little more work)

[@SuiGenerisBrewing]

No reason not to do it; just e careful you don't light the alcohol on fire.

[@meganlee8055]

What was the agar you were using??

[@SuiGenerisBrewing]

Wort-Agar. Make some wort using DME, with an O.G. of 1.004 to 1.008. Add 2% agar (by weight; i.e. 2g/100 ml; 5g/cup), sterilize and pour.