Thank you so much for such an informative and constructive tutorial. Please I am looking for how to get SNPs, Allele frequency, heterozygosity and homozygosity.
@napapornsriden8890Ай бұрын
It is really useful. Thanks a lot
@anwesasaha8321Ай бұрын
This video is really helpful, thank you very much sir.
@aljanespinas5032 ай бұрын
How to redo in fig tree?
@linuxgirl_2 ай бұрын
The video is great. The video mastering is not well done. The music is much louder than the vocal sound in the video, and the heavy base when the volume suddenly changes is very jarring.
@nirodhasenevirathna49803 ай бұрын
very useful explanation. Thank you
@teengodees40404 ай бұрын
Has anyone had the problem that fig tree is suddenly not letting you export at all? I can see my trees and Edit them but I can’t export them. If anyone has any troubleshooting suggestions I would appreciate it.
@user-lh8yw6fi1w4 ай бұрын
Did you end up making a video on getting just the coding sequences?
@juliabarrauparraga26026 ай бұрын
It is a fantastic video. Thank you so much, you are a very good teacher. I learnt a lot.
@anshul09347 ай бұрын
Thankyou sir, god bless you
@Hikl-mk1kj7 ай бұрын
i am not able to change type of my file🙂
@dogeofthesouth59988 ай бұрын
Very useful for my Biology class, thank you!
@Iamikram19988 ай бұрын
Love it
@user-pf3fi7xd7z8 ай бұрын
On exporting or saving annotation on time slot 25:08 I see that you missed to check mark the annotation box which caused to make you think the Figtree dud not work although it did the second time you tried it. I have been trying to play with Figtree for more than 10 years and just found out this tutorial but I still learned new things on annotation, fish eye, exporting and powerpoint. I have also seen ggtree but is handy, although fig tree collapses some tree branches near zero ones which Mega can split it is still useful. I do not know why there is no tutorial about this.
@mirelapale254411 ай бұрын
I just loved it. you are amazing. Thank You
@rashmibhattacherjee181811 ай бұрын
Extremely helpful video ! Thanks for your wonderful explanation...
@desaishailesh3527 Жыл бұрын
hello nice turorial, well i have whole mitochondiral sequence of 300 individual, obtained from NCBI, and after trimming i am doing alignment and its though producigng more gap and increasing sits number from 15K to 21 K, is it ohk ?
@scottkrafft6830 Жыл бұрын
THANK YOU!!! My professor had us just drop into R without telling us any of this. I didn't even know there was programming xD
@viviannwokorogu7140 Жыл бұрын
Thank you so much for this video. I love how explicit your videos are, no more boring trees !!😂
@viviannwokorogu7140 Жыл бұрын
Thanks you so much for this insightful video. Please how can I hide the bootstrap values less than 70 as reported in many publications.
@aamir9559 Жыл бұрын
How would you replicate this for 5 different genes at once? For example Cox1,2,3 cytb and RAG1 for a number of species?
@sebastianalvarezpulgarin1343 Жыл бұрын
Thank you so much! I finally understood this diagram :D
@anastasiyav4305 Жыл бұрын
Why do I have a small part of what should be displayed in Analysis Preferences (NJ/UPGMA)?
@moulaymhammedakermi3410 Жыл бұрын
Thank you for your help
@misbahmajid704 Жыл бұрын
Mega software website is not loading...I had to download it..please help
@tobyjones7292 Жыл бұрын
Does the DNA work for RNA sequences
@Carovilleneuve Жыл бұрын
Thank you so much for taking the time to do these tutorials. Your explanations were so clear and easy to understand, I will be forever grateful!
@abhipapu2720 Жыл бұрын
Sir ,why we use outgroup for construct phylogenetic tree?
@cristianjaviermena6955 Жыл бұрын
Thanks Doc! from Cordoba, Argentina.
@ivanmateotamayoceballos7308 Жыл бұрын
Saludos desde Colombia. Muchas gracias por la información!!!
@vitoriaglenzel2021 Жыл бұрын
Thank you for this, it was very very helpful!!! :D
@rajeshkannan4932 Жыл бұрын
Thank you sir, Its very useful video sir for students sir, I have a one dout sir, In fig tree what indicates scale value ? how to write a result in discussion sir
@kuilanyogeswaran1955 Жыл бұрын
Hi, Dr. The scale bar does not appear when I generate the phylogenetic tree. The distance scale box is being ticked but still, there was no scale bar appearing on my diagram. How do I solve this?
@nandagouvea Жыл бұрын
Thank you!
@wen46612 жыл бұрын
I need at least 10 sequences how can i do ?
@ayeshatripty2 жыл бұрын
How do I know wheather my epitopes fall in conserved regions in MEGA?
@williamminahua90852 жыл бұрын
Thanks a lot.
@raniam.mahmoud90772 жыл бұрын
Thanks a lot, it is very informative and helpful.
@orantiahmedomi45732 жыл бұрын
Thank you so much for making videos like this
@oreoluwaoyadiran2 жыл бұрын
Do you have a video on annotating phylogenetic trees
@sana_haron2 жыл бұрын
thank you so much sir, easy to understand, but i was here to see video on representation of tree which you have not provided yet.
@neomoonprince692 жыл бұрын
Hi, professor. Can I use different gene sequences in one tree? and how should I handle gene duplications? particularly 16S rrna?
@sizablecracker12872 жыл бұрын
could you help me, when i choose the option to open find best dna/protein, the same file save as fasta doesn't show up,, what do i do?
@DrZahidMumtaz2 жыл бұрын
You will also like this method for phylogenetic tree preparation. kzbin.info/www/bejne/r5nNh6ehip2lfLc
@miaoyi18332 жыл бұрын
Thank u a lot! It really helps a lot!!
@OsvaldoLaurindo2 жыл бұрын
Nice and clear, but please help me, I am trying to do a multiple alignment of sequences using Muscle but keeps getting an error "Stop codon(s) are found in the translated sequences. Please select a correct Genetic Code or coding frame." What am I missing?
@eswinipi2 жыл бұрын
Whenever I try to align a series of whole DNA sequences (from bacteria) the software keeps giving me an error message "MUSLCE log file did not end properly, suggesting an unhandled exception" and I am not sure what does that mean. I downloaded all my files from NCBI and they all are in .FASTA files.
@amren20072 жыл бұрын
Do you have any advice for saving larger trees with around 100 taxa in a way they are presentable and readable in a word document, when I stretch the PNG etc of my figtree in word it becomes unreadable and it’s really frustrating. The video was really helpful!
@cat-et6dx2 жыл бұрын
Figtree is nice to visualize trees. For my dissertation I had to use a package in R. There are other ways using Python. I get frustrated too.
@harry-pymdavis24822 жыл бұрын
I've had a lot of success using [the free software] Inkscape! Export the file from FigTree as an SVG file then go to town!! What I like to do is fiddle with the tree as much as possible in FigTree first to make sure I don't accidentally mess with the branch lengths when it's an SVG file (these could just be my own unnecessary habits though). I like to expand it to a point where it's visually readable including branch/node values, and thicken the branches up a bit too. Then in Inkscape it's a case of jumping between locked and unlocked aspect ratios for the tree (you can ungroup the vector file if you want to colourise specific clades or shrink the root a bit for example), and editing the font of the labels and values until you're satisfied with it all. You can set the page size to A4, then when it comes to exporting the final product I export by "selection" or by "page" and that usually gives me a nice PNG to place in a Word manuscript. Good luck!! There are plenty of tutorials on KZbin for Inkscape too, so you'll be fine!! :)
@user-pf3fi7xd7z8 ай бұрын
I have also heard about Bluescape or something ArcGIS. I am going to see which one is free. Thanks that was something good!
@bipinkb86062 жыл бұрын
I could not change my txt document to fasta even if I have renamed and if I use MEGA to save the file, it just shows MAS format, not FASTA format. As I use MAS format file to align using MUSCLE, it shows error, how to overcome this problem?
@bipinkb85192 жыл бұрын
It is Showing error message, while I go with "Align DNA" using MUSCLE. How can I overcome that problem?