mam in DPA method you told that due to DEPURINATION the concentration of DNA measured will be half of the actual DNA present in the sample then why is this not true for ORCINOL method as in this method also depurination is occuring
@ruchika_89928 күн бұрын
Thank you soo much madam 💗
@RajeshwariKotwaniАй бұрын
hello ma'am may u plz tell me from where have u taken reference for this , specially for the enzyme activity formula so that I can mention that my teachers, i cannot quote a yt video i hope u understand and reply the soonest.
@Nova-r6s7nАй бұрын
Ma'am how the OD is obtained?
@priyankamajhi2042Ай бұрын
Thank you so much mam ,ur videos are so much helpful to us 🙏😍
@shilpasshivakumar53573 ай бұрын
Mam what about molecular weight of protein
@LearnLifeScience3 ай бұрын
specific activity indicates enzyme activity by mg of protein
@dishasuratwala66603 ай бұрын
Thanks a lot maam ❤❤❤❤❤❤
@ritikaarora64623 ай бұрын
Thank you so much mam, you saved me. Kl mera exam h
@shailendravikram8843 ай бұрын
Ma'am why we are using the formula absorbance/ slope and not X= Y-C/M formula please explain
@the_little_moon4 ай бұрын
clear and useful.Thanks alot
@Neel.A.B4 ай бұрын
How is slope 0.00096
@samidhasharma82505 ай бұрын
Very informative video
@misssmile15115 ай бұрын
Tq❤
@manishathakur15035 ай бұрын
Spermatogenesis regulations is done by testosterone .
@BiologybuzzzАй бұрын
No 👎
@a.b2375 ай бұрын
Can u please provide a reference paper from where the formulae for calculation is taken.
@selviartsandcrafts57475 ай бұрын
Na
@Sanus-anime-world5 ай бұрын
Very informative video
@rehanakhatoon68156 ай бұрын
Thank you 😊😊😊
@nehasonar83166 ай бұрын
Ans please
@mariachristinaesteban47917 ай бұрын
it will be nice to do it in english or an english subtitle will be fine.
@areebazafar-bv2uc7 ай бұрын
mam please snd the link of slopes for standard graph
@LearnLifeScience7 ай бұрын
Calculation of slope from standard graph kzbin.info/www/bejne/j6PUf5eXpK5kobs
@RishikaShukla-wk1hg7 ай бұрын
Tq ❤️
@sanmatiparashar28517 ай бұрын
Thank you so much ma'am..🙏😊 Mam can you please explain me how to calculate slope..?
@LearnLifeScience7 ай бұрын
there is another video added for slope calculation
@kimuduadrustarao23877 ай бұрын
Y2-y1 which values are we took
@LearnLifeScience7 ай бұрын
two points on the line higher is y2 n lower is y1
@unseen47217 ай бұрын
Not audible
@emebetmohammed7 ай бұрын
Thank you for your explanation
@DeepakSingh-yw8vg7 ай бұрын
You mean to say that, we have to use sample after 30 minute to find sample absorbance? Please reply.
@LearnLifeScience7 ай бұрын
yes
8 ай бұрын
Thank you Mrs. Vinita
@shipsi9298 ай бұрын
0.12-0.66= -0.54 aa raha hai… negative value kaise aayi?
@YousufAliMd8 ай бұрын
Thank you so much madam, Can you please explain the Enzymatic activity of Pretease in the similar way
@snehabanger31378 ай бұрын
Thank you ma'am for this video it's very useful for my practical exam I am really thankfully😊.
@AmandeepKaur-xv7ds9 ай бұрын
Thanku sooooo muchhhh
@pakaanrouzmuhsinouu9 ай бұрын
Great explanation mam, Thankyou✨ 😂Our lecturer used these same slides of yours in lab today to teach the principle and procedure.
@LearnLifeScience9 ай бұрын
i am so glad it was useful😊
@Sarah-ix2be9 ай бұрын
The best video on this topic I've found so far! Thank you :) Did you only use the paper by Birnboim and Doly as soirce or did you use any other Literature? Thanks
@anjup46949 ай бұрын
Thank u maam
@LearnLifeScience9 ай бұрын
😊
@AnhLe-fk8mc9 ай бұрын
Best lecture and explaination ❤
@LearnLifeScience9 ай бұрын
thank you😊
@saptaparnide47919 ай бұрын
Why to multiply by 1000
@alhassanmohammedawal385110 ай бұрын
hello Maam, i have the coomasie protein assay reagent which is already prepared in liquid. do you i just add it to my sample and take the reading or how do i go about it. i want to analyse the protein content in the malaria vaccine.
@ShivaniQueenfarkandHllwPlaces10 ай бұрын
does diphenylamine contribute to the dilution factor?
@LearnLifeScience10 ай бұрын
dear, the dilution factor is considered for final reaction mixture. The reagent is prepared in appropriate concentration priorly. It doesn’t affect your calculation
@firdousbiradar761910 ай бұрын
Hello mam, Is it necessary to draw a straight line only from origin point.
@foodthirstera157010 ай бұрын
I am still waiting for its other part, Aplha gucosidase inhibition. Your research paper don't have clear method for it.
@deshmukh.official272610 ай бұрын
Cell count by haemocytometer
@nareshbarik538411 ай бұрын
Thank u ma'am 🙏
@aqsahabib125611 ай бұрын
Mam how we can drive regression equation from it
@abhyarthanabehera4802 Жыл бұрын
Thanks a lot Mam
@khushboosharma5931 Жыл бұрын
Thanku so much ma'am for this amazing explanation 🙏🥰
@shubhamaryan2972 Жыл бұрын
The best video for theoretical understanding of mini preparation of Plasmid DNA on internet...
@laphishimtilaphishimti7277 Жыл бұрын
Maam can u please teach on how to plot a graph in the graph paper im confused about the division to take when i used to plot