Lowry method for protein quantification

[@SunilYadav-kd1uq]

thank you mam, finally 1 KZbin channel to mila jo Life Science ke syllabus ko padhata h 😅😅😅😅😅😅

[@SunilYadav-kd1uq]

@Aden Eden oK

[@Shahidwani2k21_]

Ye sirf life science mai nahi aata

[@SunilYadav-kd1uq]

@@Shahidwani2k21_ but mere to kaam bn gya na 😅😅😅

[@user-lz3pw7jb7i]

Msc ke bad kya karenge

[@axelblaze3470]

i am a microbiology stud. thanks for help. you are a life saver before the night of my exam....take my regards

[@inactive392]

when I did my BSc and Msc.13 yrs back.We had no laptop, neither smartphone. Our tutor never explained like this.

[@aravindssp4519]

I completely understood the concepts.... its wonderful exactly what i was looking for!! Thank you so much

[@Taraivlogs-ct2og]

sachi bolu didi kuch jyada hi achi se explain kiyenho maja aagaya padhke pure youtube aisa explain karewala ssirf aap hin ho

[@ankurphillips1239]

Thank you very much for clearing my doubts because most of the tutorials are only upto standard, But you video is full with the comparision to the unkown sample also.

[@itsme9181]

Thank you sooooooo much! Itni mushkil se mili video thek se jo hamare salbus se match karti thi♥️

[@himanibhatt1170]

It is saving us in lockdown . Thankyou mam

[@Sanus-anime-world]

Very informative video

[@ronithakur7187]

Very helpful video

[@youareenough6148]

Good morning mam Mam, why do we make the sol. upto 1ml only?

[@khushboosharma5931]

Thanku so much ma'am for this amazing explanation 🙏🥰

[@whyBio]

Hello Vinita Vineet Here good explanation shared this with my class

[@abhipatil96k20]

Very nice explnation mam

[@neet_tutor_official]

Such a nice and helpful video .thanku mam

[@priyankamajhi2042]

Thank you so much mam ,ur videos are so much helpful to us 🙏😍

[@_thingsbyprajakta]

Thanks u mam. For teaching in very simple language

[@prasannanaga252]

Excellent explanation madam. Thank u very much

[@saraswatisingh1845]

Namaskar ma'am very well explained Lowery,s method thank you very much

[@thebiobuddy9595]

Excellent

[@sukanyadas4968]

Great video ❤️

[@rachitvaghani4468]

Why do we add reagents in blank solution? If we are to zero the reading then what's the purpose of adding reagents to blank solution (i.e. water)? Since water does not absorb any colour except red colour. We could use it directly instead dye formed by reactions. Please throw some light.

[@LearnLifeScience]

Blank tube includes all the chemical except the test sample. The blank is set by spectrometer in order to eliminate all the light which is absorbed by reagents, chemical and only light absorbtion and transmission by test molecule in test cuvette

[@rachitvaghani4468]

@@LearnLifeScience Thank you mam👍

[@tanimanikpuri2372]

Mam biuret Method mai video ni hai ky

[@youareenough6148]

And mam Thanks for such a nice explanation ❤❤❤

[@amolgangurde3144]

Thank you so much mam... really nice expansion...

[@shahirasadaf2665]

Is it necessary to use distilled water? Can we use buffer in place of distilled water?

[@RaviKumar-DXNRVC]

Ma'am protein ke OD-630 par le sakte hai

[@sheri-.-]

Ma'am bht acha samjhaya apne! Slope calculate krte hue y1 aur y2 ki values konsi Leni hn ?? Sab se pehli aur sab se last wali ??

[@Poojajundiya]

Thank you so much ma'am

[@subhradipmalakar148]

According to this readin agar mai practical perform Karu to practical successful hoga na? Mam

[@ArtsWithAisha]

Best explaination for Lowey's method

[@swapnamishra3325]

Very well explained . Thanks mam 🙏

[@surekhanetam6709]

Mam is method k reference bta sakte hai kya with paper

[@ashupawar3231]

Best lecture ❤️.. clear my all doubts in this vdo.. thankyou mam ❤❤

[@mezzzeddup]

Mam I have to make interpretation of journal i have various readings of different students this experiments how i calculate average of these readings please explain mam

[@alokmohapatra8231]

Mam, can we find concentration of Protein of unkown sample by substracting OD value of unknown sample with intercept and then divided by the slope? Is this good method?

[@LearnLifeScience]

OD/slope is a preferrable method.. Still you can compare your answer by that method...and you can also get tha conc. Directly from the graph by plotting OD at y- axis straight to line and meeting point at x axis..answer will be same

[@alokmohapatra8231]

@@LearnLifeScience Hello mam, I have diluted the protein concentration because it was showing too much blue colour, I did dilution about 10 times so how would I found the protein concentration ?

[@LearnLifeScience]

What was your initial cuvette volume before dilution?

[@alokmohapatra8231]

@@LearnLifeScience 0.5ml mam

[@LearnLifeScience]

Your Dilution factor will be 10.5/0.5 i.e equal to 21. Multiply the conc. You got by 21. It will show the conc. For 10 ml solution

[@rajashree_08]

All doubts are cleared.Thank you Ma'am.

[@Wordboost087]

Ma'am what type of question ask from this practical.. ??

[@miacara06]

Nice explanation ma'am 😍.... Thank uhh for this☺Keep it up 🙌🏻

[@mahimaaabhadrakalikildali5825]

Thank u so much mam tomorrow I m going to do this practical

[@LearnLifeScience]

Do let me know your results😊

[@mahimaaabhadrakalikildali5825]

Good evening mam I have prepared standard curve today in my lab bt all my reading coming negetive why 😩

[@LearnLifeScience]

Readings in negative means the blank tube has shown the error i think..or while setting absorbance there can be error...negative reading is not possible for a coloured solution at specific absorbance...try again and let me know

[@RakeshKumar-e1l1f]

Thank you so much dear ma'am

[@straveller4226]

@learnlifescience don’t you calculate the conc of BSA using A280 absorbance? That will give you accurate conc of BSA instead of conversion from the stock as you have explained.

[@ritikaarora6462]

Thank you so much mam, you saved me. Kl mera exam h

[@lakykhatun7119]

Mam plz explain how to prepare sample for analysis

[@rajibkashyap9813]

Madam what about reagent c. Where to add it

[@tanimanikpuri2372]

Mam biuret Method se quantitative protein determine by seed mai 1 video please

[@richakohli9653]

Very well explained. Thank u so much mam 🙏. U clear my doubts. I have problem with reagent concentration preparation and conversion of 1 unit to another. Plz help me out.

[@sharandeepkaur4242]

thank you mam ... it was of great help

[@krishnataviyad7568]

Protein % kese calculate kre??

[@Utkalbiotechvloger]

Please make a video on reagents preparation ...solid reagents/ liquids / liquid to liquid ... speci density, standard curve , titration , normality , molality ,malarity ...... factor dilution..

[@LearnLifeScience]

kzbin.info/www/bejne/bGbYiamojLV2mKs.. Go through this video, it may help you in reagent preparation.

[@sciencenerd752]

great video. thanks

[@act_pvt]

Thank you so mch for this

[@surjitdeka7092]

Maam after getting the concentration of unknown sample.. is the value obtained present in the aliqout taken from the sample ??or per ml of sample.. Can you please tell me the process of calculation after this No one tells after this Ho to calculate protein in the sample

[@LittleUniverse-5]

Thanks you for helping us

[@sharandeepkaur4242]

mam as per points taken on y axis ,.. mam the values on graph doesnt match with the one on table .

[@LearnLifeScience]

Dear, I I have plotted random points to teach. When you will plot std graph you will get straight line.

[@areebazafar-bv2uc]

mam please snd the link of slopes for standard graph

[@LearnLifeScience]

Calculation of slope from standard graph kzbin.info/www/bejne/j6PUf5eXpK5kobs

[@sakshisardana3083]

Thank you ma'am n really very helpful 💐🙏

[@mkdutta9428]

Ma'am, I had the doubt, from where did CU2+ ions come in rhe solution? Thank you so much

[@Aryan.aarkay]

Thank you very much for your hard work.

[@sharandeepkaur4242]

mam , if we take absorbance 0.180 ..... will it be correct for calculations ?

[@aminlone6330]

NICE ONE

[@sevelikaur2532]

Thanks 💖🌻

[@minotigupta6714]

Hello Mam... Mam i am getting dark colour of my sample. Which reagent i should use to dilute it? Please tell me mam.... Thank you Mam.

[@27POMPI]

Thanks a lot for such a wonderful explanatory video. We have one doubt please let me know if the final incubation after adding Folin reagent should be done under dark condition or normally?

[@LearnLifeScience]

Normally

[@manashsingha8357]

Thanks a lot mam🥰🥰

[@durgeshpatel7520]

Very nice madam

[@shrikrishna1150]

Thankyou so much for uploading such a beautiful video❤️❤️ your hardwork is seen in this video 🙏🙏🙏 keep growing 💐💐💐💐💐👍👍👍

[@lifesciencesbydr.muhammada9385]

Thanks ~~

[@yaghuvendrakumar7971]

Mam it would be better if you would have included the calculation of protein %

[@marufamalek8779]

Will u please share the link that how to calculate the standard graph?

[@LearnLifeScience]

You can use this video for understanding Construct std graph link kzbin.info/www/bejne/iHS8Z6p5j7ZlqbM Calculate slope kzbin.info/www/bejne/j6PUf5eXpK5kobs

[@kimuduadrustarao2387]

Y2-y1 which values are we took

[@LearnLifeScience]

two points on the line higher is y2 n lower is y1

[@rajashrichoure5163]

Mam if concentration of BSA is 200 ug/ml then what is concontration 0.2 standard

[@LearnLifeScience]

If conc is 200ug/ml, then standard set will be 20ug in 0.1ml 40ug in 0.2ml 60ug in 0.3ml . . 200ug in 1ml

[@Yogesh61789]

Thanks mam

[@nishant.verrma]

Heey dear lecture was absolutely great 👍, may i know which pen or tool u r using to write with hand? I'll appreciate your feedback

[@sheikdawood5097]

I hv a doubt.. Why we are comparing bsa value graph with unknown protein sample.. Because two are different proteins.. How this protein concentration calculated with bsa graph? Plz ans

[@LearnLifeScience]

Protein assays often use standards to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of the protein standard being used BSA is a protein that is universally accepted pure protein. Also the advantage of using it is its least interference with the reaction and accuracy in results.

[@sheikdawood5097]

@@LearnLifeScience thanks for reply.. Understood 🙂

[@superbscientist743]

Ma'am we also have Bradford's method. So which one is better and why ?

[@LearnLifeScience]

Accuracy of lowry method is more perfect...i have perfomed it multiple times.

[@superbscientist743]

@@LearnLifeScience Thank You.

[@tandelchandni1273]

Mam, beerlambartlaw is absorbance is proportional To proteins sample . Means more protein ,more absorbance

[@LearnLifeScience]

Yes. More the protein concentration more will be the absorbance and therefore we get straight line with increasing protein concentration.

[@divz2646]

Is this method can be used for crude protein estimations?

[@LearnLifeScience]

Yes you can

[@AnkitKumar-xz4uh]

Why do we have need to incubate ?

[@LearnLifeScience]

Incubation is done for the reaction between protein and reagent to take place.

[@AnkitKumar-xz4uh]

@@LearnLifeScience thanks

[@prajaktafokane2014]

Thank you so much mam, this video is very helpful for us. Can you please share protocol for vitamin B2?

[@shipsi929]

0.12-0.66= -0.54 aa raha hai… negative value kaise aayi?

[@architvishwakarma4803]

Thank you mam 😍❤❤

[@prakritityagi975]

Thanks for such a nice explanation. Doubt - do we need to prepare standard graph for BSA every time, we need to detect the concentration of unknown sample? Please clear this doubt.

[@LearnLifeScience]

Its better to prepare standard set along with test, to eliminate any error. Atleast 5 std tubes.

[@salamamulla1125]

Aapne kaha bsa sample 100 pr aapne likha hai1000

[@manabsenapati3996]

Thank you so much mam❤️

[@manthanjain6697]

Thanks so much mam

[@drashtisanandiya911]

Please make video on how to make solution in lab? And it's calculation like percent solution and molarity etc

[@LearnLifeScience]

I have prepared video on chemical weighing. Do watch😊

[@sakshisardana3083]

Ya shure ma'am I subscribe ur channel

[@drashtisanandiya911]

@@LearnLifeScience ya sure

[@Kanika-sq8dj]

Thank you mam

[@dewanlimboo9350]

This is very informative ! Thank you! Can you make a video on Total sugar and Vitamin A

[@himadriborah4642]

I want the protocol for xenobiotics enzymes glutathione s transferase, cytochrome p450. Please share.

[@anweshadas7810]

Can you please upload the experiment on the estimation of SGPT and SGOT or GST and GSH in serum/tissue.

[@atanusamanta3328]

Mam please reply I have data from stranded curve At Concentration absorbance 300 μ 0.08 600 μ 0.14 900 μ 0.20 1200 μ 0.27 1500 μ 0.30 And our unknown sample shows absorbance 0.16 , so what Will be concentration of unknown sample ? Our teacher say that it will be 645 μ Is it right please reply mam 🙏🙏🙏🙏🙏

[@LearnLifeScience]

As per standard absorbance, test conc. Will range between 600 to 900. Its better to calculate Slope and get accurate concentration.

[@bt-017indraniroy6]

Maam what is OD ?

[@LearnLifeScience]

Optical density 660nm for proteins.

[@bt-017indraniroy6]

Thanks maam for your response

[@subratasardar6869]

Hello mam ...... Best explanation 🥲☺️☺️

[@mahimaaabhadrakalikildali5825]

😍😍😍🥰🥰🥰🥰🥰🥰🥰🥰

[@healthandhum]

Mam plz help me 🥺🥺😭😭

[@billzon4]

are you next to a jet engine

[@NishaSharma-ve2tr]

Thanks ❤️

[@bijoymondal6005]

Mam apni to Bengali

[@surekhanetam6709]

Pls tell me mam

[@Amanda_52]

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