10 Calculating Interplanar Spacing (d) from HRTEM Image

Learn how to calculate Interplanar Spacing (d) from a High-Resolution Transmission Electron Microscopy (HRTEM) image in this step-by-step tutorial. In this video, we will go through the process of determining the distance between planes in a crystal structure using HRTEM images. This technique is commonly used in materials science, physics, and chemistry research. Increase your understanding of crystal structures and improve your analytical skills with this informative video.
#InterplanarSpacing #HRTEM #MaterialsScience #Physics #Chemistry #CrystalStructures #AnalyticalMethods #Tutorial #ResearchMethods #AnalyticalMethods

[@DnyaNiPhysicsWorld]

Note: You need to fill the dot with white color before performing the inverse FFT (kzbin.info/www/bejne/rWHVhpyOfb-Yhqs). If the fill color is different, go to the Edit menu, select Options, then Colors, and set the foreground color to White. Now, you can fill the dot with White color and then perform the inverse FFT.

[@dianavanessacorderorodrigu7627]

It was just what I needed. Such a good work.

[@sagarikasahoo2303]

I was asking my senior about this and he did not help me.....Thank you so much for making such videos.

[@MIZRAIM1984]

thank you so much! Really cool tutorial!

[@anyeshachakraborty5056]

Very helpful...thank u

[@chemistmarusaheb1296]

nice one And very helf full to me for

[@yusufahassan5833]

Good job sir.

[@khanfaizan8229]

Excellent explanation sir👌👌👌

[@nizam2017]

Nizam

[@sumansarker5186]

Thak you

[@amansirbhuworld9688]

if number of peaks are greater than no. of lines counted perpendicular to parrallel lines then what it mean?

[@akshaypatil876]

Hello sir, after selecting the angle tool first line I understood we need to draw aligned to the white line however should I draw the next two lines at 90 degrees? on what basis do I have to draw another 2 lines? please explain to me. Except for this, I understood very well, nice explanation.

[@DnyaNiPhysicsWorld]

Thank you for watching the video and for leaving a comment. The first line you drew using the angle tool is not a line but an angel, now the next step is to plot a profile but if you try so without drawing a line using the line tool you get a message "Line or rectangular selection required". This is because the profile plot function in ImageJ is used to display the intensity of the pixels along a selected line or within a selected rectangular area. The software needs to know the specific pixels to measure in order to generate the profile plot. By drawing a line or rectangular selection, you are telling ImageJ which area of the image you want to measure and the software can then extract the intensity values of those pixels to create the profile plot. Without a line or rectangular selection, the software would not know which pixels to measure and would be unable to generate a profile plot.

[@vaishalitanwar3670]

I am unable to draw a line after the angle. Please help🙏🏻

[@DnyaNiPhysicsWorld]

Elaborate the issues faced by you @ [e-mail]

[@akshaypatil876]

Hello sir after pressing Invert FFT the pop up message coming like this = No pixels have been set to 0 (black) or 255 (white) so filtering will not be done. after pressing ok same like you image not coming. How can I solve this issue.

[@DnyaNiPhysicsWorld]

The step before using "Invert FFT" is to use "Fill" for one of the bright spots, which you have missed.

[@ruchisharma-ll9mf]

I'm facing the same problem plz help

[@DnyaNiPhysicsWorld]

@@imaginarynumber416 Reciprocal Lattice Points in FFT: When you perform the Fourier Transform on an HRTEM image, the resulting FFT image displays bright spots corresponding to the reciprocal lattice points. These bright spots are indicative of the periodic structures present in the crystal lattice. Filling a Bright Spot: Filling one of the bright spots in the FFT image with white effectively means emphasizing a specific reciprocal lattice point. By doing this, you are enhancing the contribution of the corresponding lattice plane in the spatial domain. Inverse FFT: Performing the Inverse Fourier Transform on the modified FFT image brings you back to the spatial domain. This process essentially reconstructs an image based on the modified frequency domain representation. Black and White Bands: The resulting image after inverse FFT consists of black and white bands. These bands correspond to the interference fringes resulting from the crystal lattice planes. The spacing between these fringes is directly related to the interplanar spacing of the crystal lattice. Calculating Interplanar Spacing: By measuring the average distance between the black and white bands in the reconstructed image, you can determine the interplanar spacing. The distance between adjacent bands is related to the wavelength of the electrons used in HRTEM and the angles at which they scatter, providing valuable information about the crystal structure. Physical Context: Filling one of the spots before inverse FFT is done to isolate and emphasize a specific lattice plane. This step helps in obtaining a clearer and more focused image of the lattice plane of interest, making it easier to measure the interplanar spacing accurately.

[@ashwinichavan499]

@@DnyaNiPhysicsWorld even after fill option i m facing same issue pls help with this

[@DnyaNiPhysicsWorld]

@@ashwinichavan499 Can you elaborate what exact problem are you facing, what error message are you getting?

[@rasankumargiri1865]

It's a humble request please reupload the video while rectifying the mistake

[@DnyaNiPhysicsWorld]

Please check the pinned comment

[@anupsinghania7290]

How to save it?

[@dr.ketangattu824]

You can save the final image file as File-> Save as->Jpeg

[@kausarshah8903]

when i click on auto and apply it give me warning that your pixel vlaues may change plzz sir what should i do

[@DnyaNiPhysicsWorld]

Check the pinned comment

[@kausarshah8903]

i am facing deficulty when i go to adjust and brightness/contrst it give me warnning that your pixl value may change sir kindly guide me i really neend it

[@DnyaNiPhysicsWorld]

If you have followed all the instructions, you shouldn't see any such warning. However, if you are still encountering the same warning, please share a screenshot of the message at [e-mail] so that I can assist you better.

[@kausarshah8903]

thank you so much for your fast response and helping me i have emaild you screensjots

[@rcfychemistry3082]

Can u explain in Hindi..? How to interpret the HRTEM images of nanoparticles..

[@ruchisharma-ll9mf]

Plz sir give me ur email i want to discuss over result

[@DnyaNiPhysicsWorld]

[e-mail]