5) CRISPR Cas9 - Screening and Validation Strategies

[@joanacosta7258]

woaw thankssss

[@jedn6488]

Isn’t serial dilution a tedious way to isolate dna?

[@YeddulaSAIGOWTHAMREDDY]

Can we use nanopore long-read sequencing for my CRISPR knock-in experiment validation?

[@abmgood]

Yes, long read sequencing can be used for a more comprehensive view of the knock-in region of interest. Since the read length is longer, it can give a more complete spectrum of mutations induced by CRISPR-Cas9.

[@r_-_-_s8621]

Amazing Video!! Thank you :-)

[@abmgood]

Glad to hear that you found the video helpful! :) Thanks for watching!

[@jethrosims8339]

What would you use for CRISPRi?

[@abmgood]

CRISPRi utilizes dCas9 with or without fused repressor domains (e.g. KRAB) along with a sgRNA to target the promoter regions for transcriptional repression or knockdown of a gene. Visit our knowledge base today to learn more: info.abmgood.com/crispr-cas9-gene-regulation-dCas9

[@cesartopper1545]

Subtitle to spanish?

[@Oncolytic]

Not clear :/

[@abmgood]

Hello, Please contact us at [e-mail] if you have anything unclear! We will be more than happy to help you :)

[@gordonbarrett2783]

The days of Noah are here.