Great video! If I wanted to knock out a gene in C. diff by using bacteriophage deployment of the cas9 protein, complementary gene sequence, and the guide rna, how would I do so?. A few more questions are: 1. Which of the 3 viral delivery methods would be bacteriophage? 2. How would I go about designing the dna strand that I want to insert? 3. How would I get the edited bacteriophage to grow clones? 4. How can I assure that the bacteriophage will target the C. diff? 5. How can I tell if c. diff uses the Nhej method or the hdr method of dna repair? 6. What can I use to find the base sequence of a particular gene in the c. diff genome? Sorry for all of the questions!! I hope that you all can help 😁

This is really very helpful!!!

Really short n sweet

Tell me bout genomic technique at least there will work...

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Humans hijacking a virus hijacking a virus hijacking a human cell. Wow

Hi! What secondary annealing means?

Very helpful! Greetings from Brazil.

Very good guide.

I have created arms PCR primer; might I perhaps email it for revision?

PAM is located opposite strand compared to the strand where guide binds.

Fantastic, brief and awesome video, I was trying to understand the Crispr A system, it was so nicely explained here. Thanks a lot!

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Such a cute little video with lots of information. Tell me what happens to the experiments? Where do these mature “cells” go? The CRISPR-Cas9 creations?

Pq no hay elado n la cafeteria de la USJ? kobhñp noug b

great

I was wondering why you included the PGK-Puro within the AAVS homology arms? If the CMV promoter is constitutive then can't we just select based on red flourescence?

This is the best I have seen so far on RNA-seq... Thank you so much, sensei.

Thank you for the wonderful visual+explanation! 15:53 May I know does the fragmentation occur before cDNA synthesis or after? I look through the protocol from Illumina they fragmented the RNA before cDNA synthesis

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Thank u❤

Even though this video was 8 years ago, it still seems great. I love it. Thanks a lot

Excellent work. Thanks so much for sharing.

You left out the all important part everyone wants to know. The kit costs $360 USD.

I made cell immortalization part of my morning routine and was able to cut my coffee consumption almost in half!

Who, if anyone, monitors and regulates CRISPR usage? What's to stop this tech falling into the wrong hands?

Amazing video thanks.. please reduce the speed of audio.

The background music is a noise.

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Can this technique be used to generate knock-in mice?

What is the use of ColE1? does that the origin of replication for the replication of plasmid inside the mammalian cells?

A brilliant, clear and comprehensive video!

fantastic video

Hello, Can you answer this question that if non-engineered lentivirus don't have psi gene for packing then what gene they use to do that ?

Additionally the repression/activation protiens can be engineered to bind to an extention of the guide RNA's so that multiple reactions can take place simultainously. The dCas9 shown here would only be good for one reaction at a time, where all genes targets are activated or repressed but cant do both for different sets of gene targets. RNA binding components are able to do that though and is also an option.

Very well explained introduction to Adenoviruses as vectors.

Micromolar filter 😂

I taught a neighbor boy how to make an electroporator from an AM FM radio powered by 9 volt batteries.

that is great thank you!

What is meant by Tm of product?...what is meant by word product?????

THis is my first clear understanding to PCR Am very impressed Thanks a bunch

very explanatory, thank you

Thank you so much for clarifying such a hard topic in a simple language. It was very helpful.

Tq so much for this informative video

abm u r the goat for making these videos. thank u

Nice explanation. Is it safe to consider Infection unit in this video is same as PFU(plaque forming units)?

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