7. Replication

[@TIM-721]

her tone of voice is really strong and she is really confident in whatever she says. So that make me believe in every word she says

[@marianaht4366]

"Who can't be excited by a complex called the ORC complex?" hahaha Nice lecture. I really learnt a lot in just 50 minutes.

[@lukaslenart7390]

Greetings everybody just a little correction. I think the instructor used wrong term for the viruses she talked about - "Baculoviruses" are viruses of insects, not bacteria. Viruses of bacteria are called bacteriophages and the particular phage, that was used in Hershey and Chase experiment is Bacteriophage T2. I´m writing this down so you don’t make unnecessary mistake at your exam 😊 Otherwise I must say that these courses are brilliant, love the structure of these lessons as well as the wide spectrum of knowledge that gets covered in the whole “series”. Thank you very much for making them accessible for everybody 😊

[@TonyTigerTonyTiger]

Thanks. I knew the correct term was bacteriophage (or just phage), but had no idea what a baculovirus was.

[@UDITKUMAR-gh1xc]

@@TonyTigerTonyTiger same here... viruses which infect arthropods

[@DellAnderson]

Her slide was correct, but some how her words were wrong. Sometimes my brain does that too. Think I'm saying one word, and another comes out.

[@cswanson4476]

Agreed. I was listening without watching, and when she described a virus that looked like a moon lander, I thought “Oh, she’s been talking about a *bacteriophage* all along. Why does she keep calling it a ‘baculovirus’?” But I adore her lectures and I’ve been using them every evening to unwind and destress. It’s comforting to review things I used to know, and amusing to learn things I never knew. I am grateful for this series.

[@omarmath8573]

She is a really astonishing teacher; very capable of her discipline , know how to transfer the knowledge and the interest, you are rare, thank you very much.

[@sambasivaraovejendla5274]

Oh! No words. You are a legend in teaching. I love your teaching and your usage of gestures is wonderful! I became your fan! Thank you very much!

[@timiderte]

You are great. You make knowledge easy to understand. You are mastering your knowledge and you know how to transfer it. Thank you.

[@eukaryotic0703]

Amazing lecture, amazing teacher thank you. Jake

[@dr.subhojitsen6126]

A correction:12:45 onwards the speaker mentions BACulovirus as infecting BACteria - but thats wrong. its BACteriophages. Baculoviruses infect insect cells.

[@jatindheeriitb]

Right

[@Mary-bx6dz]

Yes!!! Thank you, I came here to say that too. Thought I was going crazy for a second! :)

[@bioamicos7620]

Now my only dream is to take lecture from you in person ☺️ you are an amazing teacher and your teaching style is intriguing

[@katilotfi4115]

Thank you very much for your lectures and presenting the subject so well and thorough .. Many thanks for sharing..

[@xiaoweiliu5984]

An wonderful journey in these biology lectures !

[@micaelgarcia5005]

genuinely supporting me in my lessons.

[@nafezqanadilo9150]

There is a huge difference of quality between those lectures and the ones given by Eric S. Lander. Those ones bore you to death.

[@gabygonzalezyay]

I personally like watching Dr. Lander's lectures. These lectures are great as well.

[@odditoriumleviathan8725]

@@gabygonzalezyay same… I think that his excitement and energy goes a long way too. I feel like it’s ridiculous to say, but in general I feel like MIT professors are pretty good…

[@brainstormingsharing1309]

Absolutely well done and definitely keep it up!!! 👍👍👍👍👍👍

[@Alanpie314]

In the slide shown at 31:10 there are many "typos", a C paired with another C, a G paired with a G, an A paired with an A, a T paired with a T. Presumably, this couldn't happen.

[@geansharma1999]

Thanks Mam For Molecular biology. Now my all concepts are crystal clear , now I'm able to solve all type of Qsn from This Topic 🤓💞

[@awakeningthewild]

Why rna primer dont have U nucleotide in it??? See 38:40

[@woloabel]

(On Sunday of January 29, 2023). Introduction to Biology: DNA Replication and the Process therein: 1) The Structural Proteins Within the Replicasome is marvelous and Unique to Prokaryotes as it is for Eukaryotes (Multisite about 100x at a rate of 50bp/Second [3.2 Billion Basepairs for Humans]; while the bacterium has 5 Million base pair, circular Genome [1k/Sec]); By Barbara Imperiali; 2) Complexed Protein Guided By Adenine-Thymine (AT; Two Hydrogen Bounds Rather Than Three)-Rich Sites (Origins of Initiation [ORIs]; Othewise Replication Binding Sites) in the DNA Molecule (Bounded By Chromatin [Histones in a Nucleosome]) signal the DNA Polymerase to Start (about 100 Sites at Once); 3) Biosynthetic Precursors: 1) Nucleic Acids (Radio and Heavy Isotopic Labeling and Centrifugation Processes Investigations [Nitrogen 14, Phosphorus 14, 15 for Nucleic Acid; and Sulfur 35 Proteins); 2) Double-Stranded DNA Molecule; 3) Deoxynucleotide Triphosphates (dNTP); And Protein Wise: 1) Single-Stranded Binding Proteins (SSBPs); 2) Helicase; 3) Primase with Nucleotide Triphosphates (NTPs) Or a Primer; 4) DNA Polymerase (There Being About 5 Possible); 5) Topoisomerases (I or II); 6) RNAase; and 7) Ligase; There are Chemical Patterns or Rules (In fact they are interchange with Law) DNA Replication is 5' to 3'; DNA Polymerase Needs a Primer To Start (A Complementary Sequence [15-20 Nucleotides (NT) Long]); The Control and Regulation of DNA Replication Happens Via Two Mechanism: 1) Methylation of the Histones (Epigenetic Regulation) at the C5 position of Cytosine-Guanine (CpG; Adenine Can Also be Methylated) dinucleotides of Inactivation (Conserved Epigenetic Function); and 2) Histone Neutralizaton (Positive in Original Charge) of Charged Amino Acids, Namely Lysine and Arginine in Histone Proteins; PhD Barbara Imperiali, es geht gut zu DNA Nachbildung. Heil!

[@339059331]

Thank you Professor!

[@gokulgokul-qn7du]

Great lecture Madam.

[@medicokhan1607]

I got an amazing channel finally😍😁

[@saadalmissallaty90saad64]

Wooow, amazing, very informative.

[@UDITKUMAR-gh1xc]

38:40 HERE THE PRIMER HAVE THYMINE ... i think it should be uracil because its an RNA

[@Elisha-e4m]

does she have purple tips on her hair or is that the video being weird

[@1ofthosemelodramaticfools754]

How has no one pointed out it's bacteriophage, not baculovirus?

[@grayjphys]

Is the video slowed down? It feels like 1.1-1.15 seems more natural

[@not_amanullah]

This is helpful ❤️🤍

[@not_amanullah]

Thanks ❤🤍

[@BaoNguyen-ew8pj]

"not you, not you, anyone else?" This indicates a highly polarized class lol, classic stuff.

[@saadyiahsunehrieugene3154]

i just wanna master this topic 😩

[@fightingspirit7733]

How have these videos been "introductory"

[@andrewpaige316]

Does anyone else see the purple hair on her? Is that real or is that a camera issue?

[@manasaveena6528]

I feel the demonstration of how the Topoisomerase works was a good idea but execution was a bit wrong. Opening up the strands and pulling them apart is not what Topoisomerase does, that is done by helicase. Topoisomerase just tries to straighten the helix and that increases the tension on the other end.

[@samedy00]

This is exactly what she said. The guy from the left was indeed helicase, not topoisomerase.

[@thiakann1]

LOTR reference!!!

[@ahmetbuyukumman3544]

greatness of ALMIGHT GOD !

[@quarstrongforce]

To understand how things work, students really need to read the original papers.

[@kofipapa2886]

I wonder how anyone makes the claim that they understand what she is saying. It is all talking and doesn't make the thing lucid.