A Brief Introduction to Mass Spectrometry

[@justinmacak2290]

This is how chemistry needs to be taught. There are no leaps or unexplained events in this video, it is a complete, concise, logical path from beginning to end. I am so glad there are people who understand the difference between presenting information and education.

[@jp11e3]

I swear I wish more teachers/tutors understood this concept

[@ChemSurvival]

Use the videos for learning, or as a sleep aid. It is all good with me. I'm just glad to know that they are helping you out in some way. Cheers, Davis

[@cjmfourk]

great explanation of mass filtering and mass to charge ratio. as an ms analyst this is a great teaching tool. well done

[@crytp0crux]

Exemplary explanation and presentation- It's a departure from the usual explanations. Thanks!

[@zubairsyed5570]

This is such a good explanation of how Mass Spec. works. Thank You

[@mkamareddine]

You make me want to chuck my textbook out of the window. Thank you!

[@ChemSurvival]

Thanks, Muhammad. I am glad you found this useful! That is our goal.

[@ChemSurvival]

Hi Brian. No worries. You are absolutely right! The right hand rule of physics predicts that the ions would fall away from the page or out of the page depending on the exact direction of the field lines.Showing the exact motions and the field lines in three dimensions is a tough animating challenge and is a little bit beyond my skill set at the moment, so I fudged the right hand rule to make it easier to see. I think the basic concept holds, though.

[@6202kamel]

A very good presentation of mass spectrometry concept. Thank you very much for sharing

[@muhammadsaqib7395]

David this is really very helpful, i wish my teacher could have explained it this well..

[@salvatoreferdinandoaruta7008]

Congrats for your clarity!

[@aniawo5119]

Phenomenal video and great explanations. Thank you. 💙

[@ChemSurvival]

Thanks for the comment and suggestions. This was one of my first attempts at creating a KZbin video, and I agree that there are certainly some ways to improve it. I like the suggestion of an alternate with genuine fragments. I have had my eye on this as one lesson which I would like to revisit and apply all that I have learned and developed over the past year making micro-lectures for my channel. Maybe the time is drawing near :-)

[@jarredaasen7596]

Best explanation video out there - - - Kudos :)

[@Bee-nk8nk]

Really useful. Thank you for making it.

[@natle255]

Wonderful video! Thanks for posting!

[@yasminali7578]

man i had an instructur try and explain this using a blow dryer...... thank god i found this

[@akeedasharma4249]

Awesome basic explanation 🎉🎉

[@Senthilkumar-wx1bx]

Very nice explanation of Mass spectral mechanism in animation view. Instead of your showing pink, green yellow balls as positive ions, you can try to show molecular ions, like methyl cation, ethyl cation, benzene cation if you choose Ethylmethylbenzene. I think this will be more highly understandable for youngster. Please give these kind of animation for MALDI TOF Mass spectrum. This will be useful for some higher levels... Thanks a lot for your above contribution. Congrats...

[@asifcheema3498]

good video. glad it helped.it is best explanation of mass spectrometry.

[@ChemSurvival]

Phan Chin Soon. Yes, in this simple model (and in most mass analyzers that I am familiar with) the detector is fixed and the ion trajectory is altered through the course of the experiment. This general concept is true for most common mass spectrometers, including ion traps and quadrupoles. I plan to make a few animations later showing how each of these analyzers work, so subscribe and stay tuned!

[@ChemSurvival]

My pleasure!

[@ChemSurvival]

Glad you were able to benefit from it!

[@EagleEye3]

Wow your video is so helpful. It helped me visualize the process. Hope you will have time in the near future to integrate a 3D video to portray the magnetic field as mentioned by Brian. If not recommend to add a note on the video. THANK YOU so much.

[@trinathigapanich7792]

wow, thax man, this is really good, keep it up man

[@ChemSurvival]

+Trin Athigapanich Thanks, dude. Don't forget to check out the rest of the channel and subscribe. kzbin.info

[@ChemSurvival]

Glad you found it helpful. Don't forget to tell your friends and subscribe. I am putting out new videos as quickly as I can make them!

[@ChemSurvival]

We don't. They are silent to this technique. The neutral fragments just fly right on through the mass filter unaffected by the field (no charge means no force!).

[@JohnV14]

Very well done. Thank you!

[@ChemSurvival]

+John Veitch Glad you liked it! Please pass the video link along!

[@lubnashehzad307]

Now it is easy to understand complex concepts

[@meganmoore89]

Thank you! This helped sooo much!!

[@yojelsonrc]

so once a spectrum is made you not only will know wich compound it is but also you can know how much of that compound is present in your sample?

[@Nanoscientist2]

Thanks a lot .Simple and neat

[@aminehattal7669]

Thanks a lot !!!! that was so helpful

[@mathmatters5537]

Beautiful presentation

[@alisa9626]

Thank you sir for the effort. good video

[@ChemSurvival]

+Jack Blue my pleasure. I hope you enjoyed it!

[@alex8148]

Question: For the scheme of the Mass Analyzer, since the magnetic field is applying a constant force to the fragment ions, shouldn't the arrow point downwards? Perhaps there's a reason why you have them pointing UP. Awesome video!

[@rondavis8350]

HiAlex. If I understand your question, you are pointing out that the right hand rule isn't really presented quite right. If so, you are correct. This was one of my first animations for the channel, and the 3D relationships between the field lines and particle motions was (and still is ) tough to show just right. Truth be told, there are many more common (and more complex) mas filtration schemes, but the principle still applies!!! Lighter ions are deflected more than heavy ones.

[@alex8148]

Thank you, sir! Are you planning on coming up with a video showing a more detailed explanation for Mass Spec in tandem with either GC or LC? Again, thank you very much.

[@rondavis8350]

I would love to. Will probably start intense work on the channel again this summer after my project with The Great Courses is wrapped. Based on the success of my lab and instrumentation-focused videos, I will definitely be producing more of them then!

[@akeedasharma4249]

What happens to the lighter ion when not being detected by the detector? Where are they being striked on?

[@chin-soonphan4979]

At here, the ions are separated by using magnetic field. So the detector is fixed in position and need to constantly vary the magnetic field so that all the ions able to strike the detector?

[@maheshbutta7398]

good demo..quite informative.Thanks

[@bmcpeakUMD]

Not nitpick, but I think the magnetic field should be perpendicular to the motion of the ions, or in this case, into or out of the screen

[@kobit2111]

Thanks for the vid. that was very informative. Do you happen to have one of tandem MS aka ms/ms? This would be highly appreciated.

[@easylearning981]

Isn't an eletric field that makes the ions travel till the mass analyzer? I assume it's omitted. Great video btw :-)

[@inasamad2002]

great video, thank you

[@ChemSurvival]

Thanks for watching! Please pass it on.

[@alexandernrgardalsing2370]

Very nice video. Ty alot. Regards

[@savitri7]

If I wanted to cite this video in an essay, what sources would I use?

[@swiftsword684]

what does the detector called within mass spect

[@fatimaaw93]

Thanks a lot!! Really helpful =)

[@indukumarimurugan7639]

what happens to the beam of electron that resulted in ionization of gases after it has ionized

[@concept4549]

WOW thanks so much .

[@AKASHPARAB2]

good start up for pg's thanks.

[@Tanvi009]

how do we deal with the neutral fragments ?

[@chuangqiwei2359]

very good!

[@sadiqnoor7930]

Thanks

[@sowjanyach4765]

thank you sir good and vary usefull video

[@jul8613]

Thanks a lot!

[@ChemSurvival]

Glad it helped!!!!!

[@vrushalitambe6525]

Why we can not use multiple detectors

[@ChemSurvival]

I assume you are suggesting a detection system that could detect all masses of interest simultaneously (something akin to a photodiode array in UV spectroscopy that can detect all wavelengths rather than scanning through them to produce a spectrum)? If this is indeed what you suggest, then there are many reasons. My first instinct is to site cost. The electron multipliers that my instruments use cost over a thousand US dollars each. An array of those detectors covering the entire spectrum of masses that I might want to see could easily cost more than the entire instrument does now (not to mention that the manifold of the instrument itself would have to be be gigantic to accommodate all those detectors). In addition, most mass filters don't work exactly as I have shown in the animation. In my illustration, the paths of the ions simply curve. In most modern MS systems, however, complex electrode configurations are used to make ions travel in spirals (as a quad system would) or orbits (as an ion-trap system would). These more complex ion motions have the advantage of requiring less physical space for the instrument, but they also often would preclude the use of multiple detectors. Finally, the experiments can already take place at startlingly fast speeds with modern technology. My ion trap systems scan the entire mass spectrum of small to moderate sized compounds in far less than one second. This is more than fast enough to produce the resolution necessary to create a useful chromatogram, for example when used in GC-MS. I would file this under the "if it isn't broken, then don't try to fix it" logic for not using multiple detectors. Thanks for the question! Professor D.

[@ashleyking1862]

How does Liquid Chromatography change the mass spec results/process?

[@ChemSurvival]

Hi Ashley. Liquid Chromatography (LC) is often used when the compounds to be analyzed are non-volatile (usually rather large compounds, like polyaromatics, large peptides, etc), though it can be done on any non-volatile compound. This method of separation doesn't produce a gaseous analyte the way GC does, and so a different ionization technique is often employed: the technique of electrospray ionization (ESI). As the name implies, ESI involves spraying the liquid analyte solution into the MS through a nozzle with a voltage applied to it. The molecules of analyte take on charge as they pass out of the nozzle via a process akin to the triboelectric effect. One noteworthy consequence of ESI-mode MS is that ions can, and often do, take on multiple charges, making their m/z ratios less straight-forward than they are with electron impact (EI-mode).

[@aankawlni6142]

nice presentation

[@PsnAssassinHD]

I am seriously screwed for my lab tomorrow

[@Juliossca]

nice one

[@miss1smartysoda]

I want more

[@ChemSurvival]

try kzbin.info (my channel, with over 100 videos like this one!)

[@ChemSurvival]

ehow.com shows the MLA approach to referencing online videos. You should be able to glean all the info you need from my channel (upload date, author name, etc).

[@suzanpaul3615]

ENJOY BEST

[@JDeeptheeGreat]

*Snores*

[@ChemSurvival]

Yikes! Here's hoping you were wrong about this!!!!