Acidic vs. Basic Stains in Microbiology

I also have videos on the Gram Stain, Flagella Stain, Endospore Stain, Capsule Stain and more if you want to check them out! Here is a link to a playlist on all my videos relating to Microbiology Stains:
• How to Pass Your Micro...
Biology Professor (Twitter: @DrWhitneyHolden) explains the difference between acidic and basic stains (dyes), a handy pneumonic device to help with memorization, and the uses of these stains in microbiology. Stain structure is also discussed, including chromophore and counterion descriptions and examples of multiple stains are given.

[@tiffanyscott3259]

Some people are destined to be teachers and its their God given ability. You are definitely one of those people! Thank you for making Biology easy to understand.

[@cristiano4309]

Thank you for all your hard work. With your videos, microbiology doesn’t seem that scary anymore.

[@woah1887]

Honestly thank you, I have been searching quite a lot to find an explanation to this because I’m a med student and these fundamental ideas always make anxious when I don’t know them but I’m glad I found this video THANKS !

[@BiologyProfessor]

You are welcome and GOOD LUCK with your studies!

[@francismannion7075]

That was really great, I found it easy to follow and very clearly presented, thank you.

[@trieditx8935]

thank you so much mam you cleared my all concepts about acidic and basic dyes.What would i do without you.i love sooooooo much.You are best teacherin the world

[@mehjabeen1128]

BEST DAY FOR ME BECAUSE I FOUND YOUR CHANNEL. THANKS A LOT MAM, SEEN LOTS OF VIDEO BUT FINALLY SATISFY AFTER WATCHING YOUR VIDEO ❤

[@bernadetteborja7828]

love all your videos on microbiology!

[@sujis142]

Thank you for making easy understanding of the concept with least tyming

[@BiologyProfessor]

I’m happy to help!

[@ramesh_ghanghav_04]

Superb job

[@Apple561989]

Thank you,, you are like my savior for microbiology...

[@BulbulChan5]

you are awesome. please make more microbiology videos!

[@BiologyProfessor]

Any requests? :)

[@samehmercer5627]

That helped a lot, thank you very much

[@healthcare6102]

A very nice lecture

[@knowledgenecessary3832]

It's very helpfull and easy method

[@maheshiwijesinghe682]

Thanks

[@harshmishra8108]

You make it so easier 💯💯😊💯

[@BiologyProfessor]

I’m love to hear that! ❤️

[@harshmishra8108]

@@BiologyProfessor thanks 🙏

[@dudekularehaman3902]

Thanq.. For explaining.. It is easily understandable...

[@hyder_youtube]

please upload more

[@tahirsmedicalandbiologyvid4969]

good

[@sallyrahma608]

Thank you

[@BiologyProfessor]

Thanks for watching so many of my videos. I hope they have helped you. :)

[@lazylavender3628]

Hello ( IM SO HAPPY I FOUND YOU BTW) , I'm very confused about positive acidic stains like rose bengal, may you please explain it? What makes it different from the ones you explained in the video? Thank you!

[@mrjibo]

I need some classes of microbiology can give me at zoom classes?

[@IndianBoy-1433]

Thank you madam

[@sivasankar372]

Teaching, Nice !

[@muhammadsadiq8191]

Mam will you kindly tell me azure B dye is made up of what which is used in hematology?

[@fred9za]

Awsome information thank you for making it avaliable

[@BiologyProfessor]

Glad it was helpful!

[@mehjabeen1128]

Mam make more videos about microbiology

[@ramesh_ghanghav_04]

Thank🙏

[@Newstepinstitute]

Vry good

[@jayprakashsingh5890]

Nice

[@tastedpurple1492]

Is acidic stain different from acid-fast stain? Slightly confused here. Thank you xx

[@BiologyProfessor]

Yes, they are different. Acidic stains are dyes that are negatively charged (like the bacterial cell walls) so the dye is repelled And gives color to the background the cells are on rather than color to the cells themselves. On the other hand, the acid-fast stain is a staining technique that allows one to differentiate between acid-fast cells and non-acid-fast cells. I have a video on the acid-fast stain here if you are interested: kzbin.info/www/bejne/inSpoYhupZh1r5Y

[@subhodiproy536]

Ma'am why proteins are stained by acidic dyes and nucleic acids by basis dyes ?

[@BiologyProfessor]

Acidic dyes react with cationic (positively-charged, basic) components in cells. Proteins fall into this category (they have a lot of amino acids with positive charges), and so they will bind to acidic dyes. Basic dyes react with anionic (negatively-charged, acidic) components in cells. Nucleic acids are acidic, and therefore bind to basic dyes. You can find more information here: histology.leeds.ac.uk/what-is-histology/H_and_E.php

[@Newstepinstitute]

Hello

[@Islamthewayoflife5M]

Love you from kashmir❤

[@aryanpant982]

hey in past i have seen so many of ur vedios but in this vedio u look completely changed...u hv lost weight.... by the way this vedio was quite informative .. but i have 1 question in gram staining what will happen if we will replace crystal violet with mehtylene blue..vl it give result because both the stains are basic stains....???reply me fast..

[@Newstepinstitute]

I m from India

[@motivatedyouth313]

mam plzz upload more videos

[@BiologyProfessor]

Any requests?

[@akshpatel2868]

So cute Ma'am

[@jaydhanwant4072]

Why is Emma Watson teaching biology?

[@devendrananda9850]

I love u😘