Affinity chromatography

[@tam5743]

This is still my favorite explanation for affinity chromatography. Thank you, always.

[@shomusbiologyofficial]

You're welcome

[@nusaibashafi932]

I can't express how eloquently you explain everything! Even the hardest concepts seem easy when you teach us through your videos. I've been grateful to you for years now and will surely be forever grateful to you for your contribution to the society! Lots of love and prayers, Sir.

[@shomusbiologyofficial]

I am glad to help you

[@debajyotiacharya1152]

Sir if you were not a lecturer in youtube i would fail in microbiology subject. A big thank you for you Sir 🙏🙏

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. All the best for future career

[@debajyotiacharya1152]

@@shomusbiologyofficial thank you Sir ❤

[@natasha3467]

You really make things easier, thank you so much sir❤

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@antonelle3477]

Thank you wholehearetedly for generously sharing knowledge with people! You are a true scientist! Greetings from Greece

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@erintopel2554]

Thank you so much!! It's so hard to find a clear and concise explanation of his tags, and I understood so much more after watching your video. Keep it up!

[@rimsharana2782]

God bless you sir! You make everything so easy and clear to understand! I hope one day you become a professor at a top university!

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@bornitadeb8282]

Thank you so much for your explanation. Khub bhalo bojhao tumi, Keep going!

[@shomusbiologyofficial]

You're welcome

[@wilmayuangu9386]

Thank you sir. Very clear and simple explanation. Straight to the point. God bless you

[@anushaverma6134]

your videosss helped a lottttt... nd alwayss clear my doubts.. i used to watch ur videos 1st and dn go for books ☺☺☺☺☺

[@IshikaSharma]

All Chromatography methods are so easily understood and are delivered so well👏👏👌👌👌

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@dolly7582]

Sir ! Your explanation have always been so crystal clear so far. It helps me understand in a wide perspective. Thank you so much ! 👍🏼

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@sanabiology5110]

That‘s the best video i have seen so far! Thank you so much, you are the best🥰☺️

[@AshokKumar-pg6iq]

Thank you so much sir.. From only ur teaching I am going to clear my sem xams..

[@Salikabadshareef]

hello..... hope you are doing well..... i just want to thank you because this video saved my annual exam as i dont understand how to prepare it from a book chapter. it was written soo soooo difficulte there. thank u soo much again . keep it up.... good job.

[@raikarsart8777]

I always prepare my Biochemistry notes or any doubts,i clear it by listening ur tutorial videos sir....thnks alott.... very very nice and clear explanations ....very easy to understand the concepts...

[@shomusbiologyofficial]

+shrinath raikar thank you. Glad it helped

[@kathyjoseph2105]

Shomu's Biology Hi dada, can you please do videos on Phase 1 and phase 2 reaction for drug metabolism please!! I believe you can better explain these. I really need a better understanding on those materials.

[@priyankakaushik5270]

Thanks Shomu for easiy way presentation to understand the chrom tech

[@shomusbiologyofficial]

Thank you

[@oliveromondi6592]

A good explanation can you explain concept of spacer arm in the process

[@haifa9415]

Best teacher 💯 thank you 🙏🏻

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@aleenasebastian_]

Thank u sir...it will more helpful to present my seminar .... thanks a lot

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@irsagul7978]

Thnk u so sir i hear this lecture at paper time and these lecture help us .at paper time.🤩🤩😍

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@iqimalik4658]

Thanku SO Much Sir...I Am from Pakistan ...I Have lot of Doubts about this topic.....But u use very simple way to explain ....that is easy for me to prepare this topis ... :)

[@shomusbiologyofficial]

+iqi malik you're welcome

[@shravanvansiya7100]

iqi malik special thanks

[@dejanvukovic9763]

Another master explanation!!!

[@shomusbiologyofficial]

Thank you

[@mayukhroy5393]

Thank You Sir, your video is very very helpful.

[@shomusbiologyofficial]

Thank you

[@nagayoutuberfamilyasemoi.255]

always the best teacher..you are sir.

[@chbhagyashreepraharaj5486]

Thanks sir ...u r really amazing ...❤️

[@Gayu_raje]

Your videos are so useful and can be easily understand thank you so much

[@shomusbiologyofficial]

+Gayathri Rajendran thank you. Glad you liked my lectures

[@poojaprakashtvm664]

thank u sir for giving us a clear and concise explanation

[@shomusbiologyofficial]

You're welcome

[@KIRUBAKAR]

In ELISA what should be use for solid stationary phase in affinity chromatography

[@aisyahmoktarroji167]

thank you so much your elabooration is so gooddd... keep it up!!!

[@shomusbiologyofficial]

You're welcome

[@nlnrao123]

Very helpful sir Thank you for your great efforts 🙏

[@shomusbiologyofficial]

You're welcome

[@Arodiscool11]

Watching stuff like this reminds me how much extra irrelevant information they like to pass out at uni. Everything is pretty simple when summarised to its core elements and outcomes

[@shomusbiologyofficial]

+Arodiscool11 yes. Keep watching

[@chanechimyang4863]

So true

[@barsha9831]

Your videos are really helpfull... It clear all my doubts. Thank uu sir...

[@prabhanadukuri5428]

Supr explanation sir I like the way u teach its very much helpfull in proper understanding

[@kavyap4453]

Thank you for the amazing lecture

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@sadiamallick8231]

Its very easy to understand...thanku shomus sir

[@ruby-Rubab]

Sir your classes r excellent n helpful!! Can u please suggest some good books for concepts n notes?

[@okccitizen4400]

Great lecture 👍🏽

[@shomusbiologyofficial]

Thank you

[@ramlabasharat8238]

Really helpful. Thank u👍

[@shomusbiologyofficial]

You're welcome

[@alishamoazzam7470]

great job...may Allah bless u always with all His blessings...keep the great going :)

[@shwetaathwani4756]

thanks these videos are very helpful to me as a basic understanding

[@soumyaranjanmahanta9127]

Thank you so much 😊 Sir 🙏

[@shomusbiologyofficial]

You're welcome

[@shankark7341]

nice lecture to clear the problems

[@arpitbondade2230]

sir u explain all techniques very well

[@puskaraditya1117]

today is my wbut exam .i am preparing by wathing your videos

[@shomusbiologyofficial]

+PUSKAR ADITYA all the best for the preparation

[@Niharikaswarna]

Thank u so much i love u r classes

[@shomusbiologyofficial]

You're welcome

[@vinayakmore5691]

Thanks a lot sir all your chromatography videos are very useful to understand the concept of chromatography in detail 🙌

[@shomusbiologyofficial]

+Vinayak More thank you. Glad you liked my lectures. Please subscribe and share

[@vinayakmore5691]

Shomu's Biology yes sir I already subscribed your channel and shared with my friends also 🙌

[@davidwong2010]

Hi Sir, can u explain why certain proteins need a Fc tag to be purified? Does the Fc tag have affinity to the variable region of the antibody? Thank you!

[@shomusbiologyofficial]

Fc tags have constant regions which can be used to adhere and purify antibody proteins

[@davidwong2010]

@@shomusbiologyofficial Thank you sir!

[@lisaross4002]

Sir amazing👍

[@shomusbiologyofficial]

Thank you

[@mayapatidar3543]

Sir u explained precisely..🖤🌻

[@shomusbiologyofficial]

Thank you. Glad it helped

[@shivakishor1033]

Sir u r helping me a looot.

[@shomusbiologyofficial]

You're welcome

[@郭佳鑫-n8c]

big thanks very useful❤

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@martinamiglietta6843]

Thank you for your videos Sir, you are really helpful. I have only a question for you: which technique do you use to separate antibodies from antigens attached to agarose?

[@sonamsingh-ez3hn]

Sir am your big fan ur lactures are awsm

[@kamrunnahar5309]

Good one ! Please use a microphone for better sound quality!

[@anushaverma6134]

sir .. you are just brillient

[@seshunarendra6832]

Nice Explanation Thank you Sir

[@shomusbiologyofficial]

You're welcome

[@sammrayounas2681]

Sir plz made a video on transition metal....

[@gayatriasawa2721]

Sir, I request you to make video on selective adsorption too

[@shomusbiologyofficial]

Okay

[@gayatriasawa2721]

@@shomusbiologyofficial Sir you're just replying okay but not making video on most of the topic we suggested....so please upload the video as soon as possible 🙏

[@priyachhabra215]

Thanku so much sir. Really helped me alot.

[@shomusbiologyofficial]

You're welcome

[@mahaboobbasha8208]

Informative

[@shomusbiologyofficial]

Thank you

[@sylvenafernandes5720]

Sir you are really awesomeeeeeeeeeeeeeeeee

[@shomusbiologyofficial]

Thank you

[@kaneezfatima6732]

Very nice sir thank u so muchhhh

[@shomusbiologyofficial]

Glad to hear that you are getting benefit from the videos

[@jameschimidjibril2089]

Thanks for your teaching

[@dhanadhanu7927]

Nice teaching

[@AshokKumar-pg6iq]

Super anna

[@bhargavmohan5387]

This lecture was good

[@worldshootout3298]

Tell about HIC hydrophobic interactions chrometography

[@sumondas8946]

stti shomu da tomar tulona hoi na, stti osadharo tmar video gulo

[@abbasmiya5555]

Superb

[@20UBC113OviyaRavichandran]

Clarkes oxygen electrode video panunga

[@chanechimyang4863]

U've been great help to me.

[@manojkumarsatpati2241]

nice lecture...........thank you sir

[@jasminesong9802]

Thank you so much!! Can I ask one question? If I set up an affinity column using antibody and antigen, can all the antigen be isolated? In other words, is 100% recovery possible?

[@Chem4students]

Yes brother It is possible to recover all components by choosing suitable ligand as adsorbent material I started a new channel with inspiration of shomu sir. If possible, please watch my channel

[@yogitha4147]

Thank u anna

[@shomusbiologyofficial]

You're welcome

[@surabhiambastha9631]

Superb

[@shomusbiologyofficial]

+surbhi ambastha you're welcome

[@khushbusharma5593]

Awesome 😇

[@shomusbiologyofficial]

You're welcome

[@princeyumnam6980]

good lecture

[@shomusbiologyofficial]

Thank you

[@rashmikulkarni4997]

sir plz make videos on genetics & evoulition I understand ur teaching only not that other sir videos plz plz I request

[@aniketpatil8047]

Please tell me in my proteins solution i have enterokinase enzyme so i have to remove EK with affinity chromatography? Which resin which buffer how ek bind to resin plz explain me it help me lot

[@gutemajebe4365]

I WOULD LIKE TO KNOW ABOUT Ni-NTA agarose

[@sree7834]

Thank you so much sir!

[@chanechimyang4863]

Exactly what kind of molecule is used to elude the target molecule in case of nickel-histidine interaction?

[@suprabahijam]

I can't find bio material about affinity chromatography

[@SMSQUARE899]

Osm...bro keep it up

[@aboutstudies123]

Thank you Sir.

[@arupborah57]

Sir you are grat plzz make videos on gel filtration chromatography

[@senolgundogan4787]

many thanks

[@bhagyalaxmibb9444]

why the glycoproteins has affinity towards lectin ?

[@dr.vikramchoudhary3270]

Plz upload electrophorosis video

[@ranboianimations6125]

How components from serum sample can be separated? Is it by affinity chromatography or by immunoelectrophoresis?

[@Chem4students]

Both techques useful for separation of serum components. Time & cost of analysis varies. Affinity chromatography is taking less time to separate components than electrophoresis. I have started new channel by inspiration of shomu sir lectures. Please watch my channel.

[@khadijabano1143]

Sir may u provide notes for these chromatography topics🙏🙏🙏

[@PunjabiRDX.]

Affinity purification mass spectrometry pae lecture bnye

[@rajaaelmansouri8849]

thank you sooooooooooo much sir

[@sciencevision8180]

Thnku

[@shomusbiologyofficial]

You're welcome

[@saumyagupta07]

What is the mobile phase in this type of chromatography?

[@Ana-ks3je]

He says it's liquid, like blood for example

[@Chem4students]

Usually buffer solution like tris buffer, pyridine buffer, etc I started channel with inspiration of shomu sir. I did video on ion exchange chromatography.

[@nidanasiha3983]

too much learng frm ths video

[@Ros2283]

Damn YOU ARE GOOD!!!

[@martinamari558]

That's was understandable sir... But what about the column and batch chromatography... Those r it's types right😎

[@shomusbiologyofficial]

Making new videos soon

[@abdulahad6082]

THANKS

[@mmww_]

thanks