Gas chromatography | GC

Gas chromatography is a chromatographic technique used for the separation of
volatile compounds. The volatile compounds are the compounds that easily gets
vaporised at room temperature. Separation occurs based on the interaction of molecules with the mobile phase and the stationary phase. The less volatile molecules interact more with the stationary phase and moves slowly
where as more volatile molecules interact more with the mobile phase and moves fast. When the sample is detected the computer gives a peak with respect to the retention time of the sample.
The area under the peak gives information about the concentration of the sample.

[@prangyadas5127]

A detailed information in a very shorter period of time and was explained in a crystal clear manner. Excellent.

[@sayurirathnayake48]

Thanks a very lot.i studied A to Z clearly from this video.it's amazing how you explain them simply.please make more & more videos.Good Luck

[@pepe6666]

oh dude thank you. finally a way to understand this. the terminology of a gas chromatograph is used so much with mass spectropy that its difficult to figure out whats what. i had always thought those peaks on the chart were speaks on a spectrum! this has cleared a lot up for me, thanks.

[@prayoga4419]

Very brief and to the point. Love it. Thank you so much.

[@tuffel03]

Thank you this was the exact format and presentation I needed

[@sarahholland5980]

this is amazing. so simple and clear. thank you!!!!

[@mustafeabdiazizismail3771]

Thanks 🙏 to your efforts, we finally understand the different types of chromatography

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[@sydneym3251]

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[@henrycalhoun1119]

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[@sarahkamau421]

Very well done! You're really helping us beginners! Thank you!

[@sequeira7330]

summary of the video: GC is used for the separation of volatile compounds( compounds which are easily vapourized at room temp) Components of GC: 1) SAMPLE is mixed withe a highly volatile compound like Hexane, cyclohexane, etc. • Long coiled column: can either be a packed column(made of glass/stainless steel) or Capillary column (made of fused quartz). this column is placed in a CHAMBER in order to facilitate a uniform temperature. (HIGH TEMP) During analysis, placed at 150-300°C • the SP is packed in the column. it is usually made of silicon grease or wax as it can withstand high temperature • Presence of a SEPTUM to inject sample just before the chamber - the temperature of the injection region is 20-50° higher than chamber. this is to facilitate the rapid vapourization of the molecules. 2) Mp: usually helium / nitrogen (inert/unreactive gases are used) are placed in a cylinder connected to the Column via the Molecular sieve. 3) Molecular sieve is used to filter out unwanted HCs, Water vapour, oxygen etc that may interfere with the sample during experimentation. 4) Detector

[@sequeira7330]

Detector - common: FID ( FLAME IONISATION DETECTOR ) which has 3 inlets: • carrier gas from the column • hydrogen • oxygen ignitor ignited the H2 and O2 to produce a flame. when sample molecules reach the flame, they get ionized and electrons are released. across the flame, there are 2 electrodes ( cathode for cations, and anode for anions) the electrons generated are detected by the electrodes in the form of current and it is amplified by the amplifier/Computer.

[@sequeira7330]

the computer then generates a graph: X axis- time y axis- current based on known samples and comparing their output with the output of the unknown samples, the retention time/concentration can be deduced

[@nh-vt5ws]

​@@sequeira7330 seems like you're a technician of GLC... Excellent explaination bruh🎉

[@chandrashekarn5376]

Good explanation sir with animations 🙏🙏

[@aga4966]

Really good video, made me understand GC!

[@table2790]

You are absolutely amazing! Thank you So much :))

[@Boooommerang]

Excellent video! Thanks

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WOW! I am still repeating.

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Wow online me itne marks.😜

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This video totally cleared everything up, thank you so much

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[@hysysplusunisimandchemcad4499]

Excellent Work, Thumbs Up.

[@temmietemmieson6756]

Really well explained thank you!

[@divya.t.george6030]

very well explained understood it greatly

[@Carmelo12320]

Thank you exactly what I needed.

[@shynosalexander9200]

This was very helpful. Thank you :)

[@bankthanadorn4598]

Well explained, better than my lecturer tbh thankss

[@palanirajramalingam1131]

WELL EXPLANATION EASILY UNDER STAND THANK YOU

[@triciajones6923]

So very good.Thank you!

[@mdraselmiah3403]

good explanation and it will help me more..thanks

[@sibghatkhan3986]

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[@AntonioAdame1]

Great vid. Thank you

[@themoreyouknow585]

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[@JERKINSRICE]

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[@wen6519]

That was helpful, thank you for sharing

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impressive..beautifully explained

[@jono2167]

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Thank uuu sooo much for this wonderful explanation 👏👏👏

[@arbazchaudhary5752]

thanks for this video you explained very well

[@MrKpy86]

I work for a company in the Chemistry industry that manufactures instrument and that was pretty accurate, good job. Only thing is inlet is at 20-50C higher than the max temperature reached by the GC and there is also an inlet of Makeup Gas for the FID which is usually to compensate the fact that nitrogen or helium coming from the column is usually much lower than the other gases. Other than that, great video

[@rabiaameer8588]

Amazing lecture clear my all ambiguous points about GC

[@mustakim2144]

Thank you!

[@imtme]

thank u, u are so helpful, more helpful than my lecturer

[@jayliu7786]

i have a organic chemistry lab tonight and hoping that it goes well. thanks for the video, it's very insightful :)

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Respect 🙏 from Africa Zambia better than my lecturer for a year thanks I know love this course❤

[@ianedmonds9191]

Excellent. Thanks for that. Luv and Peace.

[@zahidhussainzaigham9223]

Well explained briefly. Thanks

[@Ara2061]

thank you.. .thank you... thank you

[@shourovhossain8498]

Amazing.....the video clear the concept of chromatography.

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Thank you Sir.❤️🙏🏻

[@_sri_702]

Thank you 👍

[@dimitrikolotauri6622]

thank you sir.!

[@esterindahkusumaputr]

Thank you for your video, it's clear

[@mariofelipequevedolozano3659]

Thanks a lot!!!

[@ValeriaGobjila]

thank you for this informational video on behalf of all science students :)

[@sujikumar7831]

Super..very useful.. simple

[@bijiteshchoudhury8274]

Well explained Thank you

[@praveenkumarkp4084]

Very helpful 👍 plz do classes of HPLC and spectrophotometer

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Thank you

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[@mumizashahid6047]

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Thank you. Very helpful

[@jignasachiramana8269]

really helpful !

[@bhaveshthakor4219]

Amazing explanation

[@user-jd3bk2wq6m]

In bad need for applications of gas chromatography 🥺💔

[@user-dq9ty5tq1z]

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[@juliana_jesus]

Thank you very much!! May I have the references for further reading please?

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[@user-wt9gv3kd3q]

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Hiee

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Thanks😍

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tq ...Vry useful

[@khushbujha4957]

As we can say in 6min our separate sample is there??

[@seeker8906]

Thank you Sir

[@Matteo-mx1sz]

thanks

[@mairaseiilgazy7510]

Thanks

[@zahidhussainzaigham9223]

Respected mam, kindly make videos on spectroscopic techniques and cover all thier types separately. Thanks

[@seripahnyambura2782]

Thankyou

[@ianmatthewkline8279]

Okay but what about when two samples have similar or even the same retention time? I use LC-MS/MS as a main tool for my PhD work but I was curious about how this other technique works.

[@1987Wulf]

You can use a different phase to achieve different RTs. There are some that will never work on GC. For instance, M,P-xylenes are not chromatographically separable. However, using a HRMS will differentiate the identifications.

[@niazali5861]

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Amazing :D

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[@w.a6369]

Well explaination