Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 2 | MIT 7.01SC Fundamentals of Biology

[@appybee]

This professor makes science exciting no matter how old you are!

[@franciscovessani6720]

TRUE!

He is the master of genomic knowledge. The legendary Eric Lander, the director of Human Genome Project.

[@ptgrenville75]

This is how science should be taught. Teaching the experiments that led to the concepts rather than just teaching the concepts themselves without any context

[@rosacosta4091]

This instructor is a life savior! Thank you so much for your lectures Mr. Lander

[@mahmiiranda]

Thank you for this wonderful class. I wish there were qualified teachers as well here in Brazil, because my molecular biology's classes were very disappointing and I could only understand the subject after seeing this video. Thank you so much, success sir Eric Lander!

[@aoifewest]

best lecture of this kind I ever had; studied in France, Germany, Ireland and I am really understanding and learning from him, Thank you so much.

[@nobitte]

I'm as jealous as i am grateful that i don't have Professor Lander asking me questions in class... I'd clam the hell up quick smart. Such great lectures

[@TheFirstBK]

Straightforward, thank you Professor Lander and MIT!

[@mallikarjunbangale7990]

Blessed by this lecture

[@llewellynhamiltoniiim.d.1057]

Reminds me of my Genetics Professor 50 years ago. Interesting.

[@ScattMatt3000]

Bless your soul

[@yanpingwei6519]

He recombined the basic knowledge of molecular biology in a logical way and maked it understandable and interesting~Thx Pro.Lander~

[@Chaunguyen1502]

thank you Professor Lander

[@debayan8221]

Prof. Eric Lander... take a bow

[@mariaph9700]

thank you professor Lander. You are truly inspirational, thank you

[@marlenesoifer7219]

God bless you I'm back must put 2 and 2 together thankyou

[@HafizahHoshni]

Lecture 16 : Recombinant DNA III Thanks !

[@garynicholl4235]

36:57 applications of PCR

[@nahulseyon54]

🙏Thanks a lot...

[@NicholeRojas-r8i]

Wow! What an excellent professor!

[@kinzakhan9456]

keep posting wonderful and amazing content and make possible quality of education for everyone. Admin please keep going. bundle of thanks

[@skiingowl]

Thank you. Clear and easy to understand.

[@SeaJay_Oceans]

Thank you MIT. I enjoy all your classes ! :-)

[@damsell02]

amazing lecture, simple and clear. I wish my lecturers were such when i was in grad school. oh well never too late to gain a lil knowledge

[@xIbra25x]

20:14 having a stop codon in an intron would still be a problem because the polymerase would stop before finishing the transcription of the whole transcript wich is then supposed to undergo splicing

[@ReparentingDiary]

First question: where does the primer come from? Answer to the first question: insert it to a vector which has a different specific RE cut site a couple of base pair upstream to the gene of interest and use that sequence as a primer.

[@ReparentingDiary]

Second question: if we have to sequence a long gene for example has 3000 bp, we have to use a longer primer? Answer: can do primer walking or can cut the 3000 bp gene up into smaller fragments and subclone these fragments and do overlapping assembly aka shotgun sequencing

[@ReparentingDiary]

How do you know you recognize a gene: look for open reading frames (this is done for not complicated species) or we can make a cDNA and then compare the sequence of your gene of interest with that of another organism

[@ReparentingDiary]

4th question: how do you get a gene of interest from a second individual from the same species(aka you know the sequence of the gene of interest of individual A): do PCR!

[@transon6655]

Primer is just a small chunk of RNA which are complementary to the original DNA. Since we know the DNA sequence, we know the Primer sequence, type in the automatic synthesis machine and BOOM you have your Primer.

[@RavjiPindoria]

Wonderful Lecture - Thank you for sharing.

[@JimBlakeLectures]

great lecture ! At 26.21 it is over 6 billion bases - 3 billion base PAIRS

[@arthuralmeida3319]

What a Excellent professor

[@floramanful]

Many thanks. This series is amazingly helpful. Just a little suggestion. I turn my speaker to its highest volume, it is only at an acceptable level to listen clearly. So maybe a little improvement in recording can help.

[@SirajUddin-gs8vi]

Just loved your lecture sir....❤

[@songthanh896]

Thanks Prof Lander

[@ruthzafar7272]

GOOD LECTURE 👍GOD BLESS YOU TEACHER 😊

[@RBG02005]

Well done. Have been wondering how the whole genome shotgun sequencing works compared to primer walking.

[@eclaireurspatial]

This is amazing! Thanks for uploading these lessons.

[@rahulparmar2456]

I'm gonna meet this professor

[@thetechnik]

this prof is amazing

[@yanpinghuang8154]

thank you for sharing.

[@panjwaniricha]

amazing teaching...flawless...:-)

[@irfana19nov]

Amazing lecture

[@sarahassanraoof3930]

I envy your students , Thanks Mr. Lander

[@NickFarrow]

Function, Gene, Protein is a great cycle many dreams will be created. ;)

[@stuartlutimba6867]

Nice lecture keep sharing more

[@523101997]

how do you add a primer onto a gene?

[@franciscovessani6720]

man, this guy is clever

[@lucamoi474]

how we know where the restriction enzyme cut, before sequencing?

[@Isaiaswolf66]

+LUCA Moi Normally the catalogue gives you a list of enzymes and gives you a map of where it will cut. Google , nebcutter.

[@Wahrscheinlichkeit]

awesome lecture... thank you

[@greggrizzla9169]

Is it really true that stop codons occur in introns? How would that work? Does the ribosome know that it is transcribing an intron and therefore ignore stop codons? I don’t get how this would work to be honest. Other than that I would like to say that I truly envy those who get to take Eric Lander’s classes, he is one of the best biology teachers that I have ever seen.

[@manouchershirbacheh244]

these are great but i have difficulty navigating the series, is there somewhere where these lectures are combined or sequenced such that i find the next lectures?

[@mitocw]

These videos make more sense when viewed through the course on MIT OpenCourseWare at ocw.mit.edu/7-01SCF11.

[@kilazasamson7249]

Nice lecturing....

[@nilsgreinert7088]

kann ich nur empfehlen*------*

[@bonbonpony]

34:54 How does one extract just that particular enzyme (DNA polymerase) from a bacteria?

[@shashiprakashsingh214]

just clone your gene with tag..so simple.!!!!!

[@ushagarg3792]

Thanks Sir

[@tolibelom]

Does anyone know what textbook was used in this class?

[@mitocw]

The following textbook was used when this course was taught on the MIT campus: Campbell, N. A., J.B. Reece, et al. Biology. 8th ed. Pearson Benjamin Cummings, 2008. ISBN: 9780805368444. For more information see the course on MIT OpenCourseWare at: ocw.mit.edu/7-01SCF11.

[@lucamoi474]

how we know the sequence from the vectore which we use it a primer if we dont know how to sequence?

[@jesco6]

I think it's too late now, but anyways 😂 We know it's a sequence recognised by a specific restriction enzyme, if we know what enzyme it is and we know the sequence it recognises we just use that same sequence as the primer

[@quantumcrash7266]

Would love to grab a beer at the tavern and talk about crazy science shit with this prof!

[@amberdelgadillo9461]

As a UC Berkeley student... i shouldve gone here instead 😕

[@sxyngel]

wow tnx

[@anuvindkeezhatoor5949]

LOVE UUUUUU

[@sweetsriracha2583]

p poli, mer chay ne reatcion

[@krightonzilon9140]

What is a CATALOGUE that you are talking about. How comes we find something from the catalogue?

[@alphonsedhasantony4306]

14:22 XD XD

[@pauld4355]

datude sandsrom

[@МихаилД-ч4з]

37:25 PCR need for COVID :-)

[@EliotMcLellan]

his schtickt:::: REPEAT YOURSLEF ...... AND THEY ARE ALL FUNCTIONING ON THAT EXACT SAME SORRY LEVEL -----?>>>>> SOMETHING TELLING TO THOSE 'SORY' DYNAMICS

[@anneka3980]

I am a beginner and I find his method engaging for my level. He is clear and I enjoy following the process along.

[@mohamedomar8018]

Nice lecture keep sharing more