Overview of Recombinant DNA | MIT 7.01SC Fundamentals of Biology

[@ncertscience767]

it's my good luck to see MIT classes from India. Thanks to MIT opencourseWare to give this opportunity..

[@Bob-fj7lr]

The end scared the shit out of me but I've been doing so much studying the last 3 weeks I just needed that lmao

[@xxnl510xx]

Wow I honestly thought he was gonna have to run for the emergency shower or something

[@AmenIamHotepRA]

Through genetic engineering, bacteria could be designed to perform any function; the same is true for viruses. For instance: "Direct stimulation of T lymphocytes by immunosomes: Virus-like particles decorated with T cell receptor/CD3 ligands plus costimulatory molecules," (Immunosomes= immune cells envelope proteins or virosomes... ) It is the ligand of the virus that binds to CCR5 delta 32 receptor of the host t-cell. E. Coli is the most common bacteria known to cause blood infections...

[@HafizahHoshni]

Overview of Recombinant DNA, Excerpt 2 (00:22:17)

[@peachesandsunshine9]

the HIV virus is not a bacteriophage. Number 1. HIV infects specific immune cells. Number 2. HIV depends on a reliable host with a lifespan that typically outlives E. coli given the virus's long incubation period. Also to point, HIV transmission happens in a variety of ways

[@AmenIamHotepRA]

In the presence of another viral strain, recombination occurs a second time giving rise to a third strain. The expression of viral DNA or RNA-- following the hijacking of a viable cell machinery--- is by definition a function of DNA/ RNA governed by biological law. Nonetheless, my hypothesis [based on the lecture etc.]is it seems plausible that e. coli might have been genetically manipulated to create a vector--- for the delivery of viral genome into T-cells, as it relates to HIV.

[@Felipe-53]

THANK YOU MIT!

[@FindingGreenOS]

AHHHH I LOVE SCIENCE!!!!

[@AmenIamHotepRA]

Given the scientific definition of a Virus, what vector is used in order to transfer the DNA sequence of HIV into T-cells of the host? Also, can you identify the organism or cell that's responsible for transmitting the HIV DNA if-- as you've said-- it's not a bacteriophage?

[@AmenIamHotepRA]

What I'm presenting is outside of the box ideas based on what I read in Retrovrus Biology and Human Disease by Robert C. Gallo & Flossie Wong-Staal 1990 Emerging Viruses: AIDS And Ebola : Nature, Accident or Intentional? by Leonard G. Horowitz, KZbin video by Dr Robert Strecker: "the strecker memorandum" and other General Biology & Microbiology college classes I've taken. These classes doesn't in anyway provide the information i'm seeking or that's found in Dr Gallo's or Dr Horowitz book.

[@AmenIamHotepRA]

If you know for certain (not based on opinion) that E. coli is not the vehicle used in the transmission of the HIV DNA into the T-cell upon docking its receptor site ( ccr5 delta 32) , then what is? Viruses on their own are unable to perform any function, therefore a living organism is needed. What organism that has a symbiotic relationship with the human body would provide the machinery for recombination? Bovine leukemia virus sequence etc. recombined in this vector could cause infection.

[@HafizahHoshni]

Hahaha. Seriously I thought the end was real. I was surprised when the lab assistant brought in the pathogenic bacteria and he drank it. Lol.

[@michalchik]

What makes you think HIV has a vector. Most diseases don't.

[@michalchik]

I know the feeling, I deal with hundreds very bizarre ideas every year. It is amazing what is out there. If you want a few good laughs I can point you to some videos.

[@AmenIamHotepRA]

Sounds like a lysogenic cycle involving a bacteriophage to my, based on your statement. Lentiviruses do remain dormant for long periods, which brings into the equation zoonosis. I do understand the enzymatic mechanisms that are involved in retrovirology, however, I'm trying to determine-- based on extensive use of e. coli in recombinant DNA-- if it is possible that E. coli was manipulated to initiate HIV infection in any way.

[@srimuharyati2387]

Menarik. Thanks

[@useyournoodle100]

I really enjoy this series but i just can't stand the chalk board. Really, no overhead projector? No document cameras?

[@AmenIamHotepRA]

Gregor Johann Mendel (07/20/1822 - 01/ 06/1884) was pioneering with his early work in genetic engineering on plants. Francis Peyton Rous (10/5/1879 - 2/16/1970) was involved in the discovery of the role of viruses in the transmission of certain types of cancer.1898 mycoplasma was used to cause epidemics in cattle. In 1972 Paul Berg created the first recombinant DNA molecules by combining DNA from the monkey virus SV40 with that of the lambda virus.. 10 yrs before the advent of AIDS.

[@bobagopaa]

get a load of this guy

[@AmenIamHotepRA]

"Many infectious viruses coevolved with the vertebrate immune system. During the assembly of enveloped viruses, lipid ordered domains of the host cell plasma membrane, called lipid rafts, frequently function as a natural meeting point for viral proteins. The role of lipid rafts in the organization of complex combinations of immune receptors during antigen presentation and T cell signaling is widely recognized." Specific genes (through manipulation) could cause this to also happen in bacteria.

[@SpiritualSpace432]

Alina Gavrilyuk well if you're so clever why waste time and watch this?

[@michalchik]

Frankly I don't care if you ignore me, but I do care about facts being right. Most diseases spread by contact, water or air. STD's like Aids all spread by intimate contact only. They have no vectors. Cold and flu and measles all spread by air. Cholera and giardia by water, etc,,,

[@AmenIamHotepRA]

Could it have been through genetic manipulation of e.coli, that the HIV virus synthesis was made possible? Considering that the bacteria e.coli is found mainly in the gut--- which includes the anus--- and the transmission of HIV occurs through anal intercourse, it is reasonable to conclude that e.coli is the vehicle through which infection occurs.

[@t5e2]

depending on which type of engineering, some are more boring than others

[@peachesandsunshine9]

I am glad the feeling's mutual. Oh please do lol.

[@sumedhchipde6097]

12:58

[@parvathavarthinimadhavan2777]

Thanks for making me laugh ❤

[@someshskanda5029]

Its insane...

[@AmenIamHotepRA]

If your responses were legitimate & academically engendered, this question would not have been asked. Therefore, review General Biology 101: lysogenic & Lytic cycle.If after reviewing the two methods of viral reproduction and you still choose to be persistently diametrical in your opposition, then we should agree to disagree. Also, If you are unable to elevate this dialogue & continue presenting vacuously unsubstantiated statements & questions, I'm afraid you will be ignored. So Choose wisely!

[@CatAiLin]

I was like Are they stupid at MIT???? hahaha

[@AmenIamHotepRA]

It is the HIV vector that I'm trying to determine.

[@HarpreetSingh-gv4lo]

🤣🤣🤣apple juice

[@skiptaker]

what a boring specialist the engineering is more interesting and also more complicated

[@worldtodayhowitis5083]

absolute un useful