Agarose Gel Electrophoresis of DNA fragments amplified using PCR

  Рет қаралды 672,084

Schools Project

Schools Project

Күн бұрын

Пікірлер: 136
@callyx5783
@callyx5783 4 жыл бұрын
Because of COVID my science course at college are unable to do certain experiments like PCR and gel electrophoresis, so this account is a life saver for my assignment on them. Thank you!!!
@godislove8740
@godislove8740 4 жыл бұрын
Are your labs being used?
@maryprojectsph
@maryprojectsph 3 жыл бұрын
@@godislove8740 nnnnnnn
@maryprojectsph
@maryprojectsph 3 жыл бұрын
@@godislove8740 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnñnnnnnnnnnnñ9n
@Entertainmenttz360
@Entertainmenttz360 5 жыл бұрын
Thank you so much, i did it with lab mates and then come here watching it, completely clear understand it, be blessed much!!
@Anchal187
@Anchal187 3 жыл бұрын
Are you in college or school
@zmazn
@zmazn Жыл бұрын
absolute lifesavers, i had this experiment as my lab report and this video helped me like no other ❤
@747070
@747070 6 жыл бұрын
Never understood how electrophoresis worked by just being told about it, nice to see it for real. Thank you, very thorough video! :)
@aayushshroff03
@aayushshroff03 4 жыл бұрын
Do u see Newton before studying his law?🙄🤔😄
@GunCops
@GunCops 3 жыл бұрын
Electrophoresis is the separation of molecules with the use of an electrical field. Every substance, usually a nucleic acid or a protein, has a pI. That's the Isoelectric point and it's a feature that comes from the amount of positive and negative radicals it contains around it's perimeter (if it's a protein) or in it (if its a nucleic acid. Positive radicals move towards the negative pole of the field and negatives - to the postive. If you submerge a gel with the proper pH containing these molecules in a medium that allows an electrical current to pass through, the current will pull the molecules along with it. Heavier molecules stay behind, lighter move faster, thus, a mixture is seperated. Now, the molecular weight (how heavy they are) of a molecule is also its signature. You identify the molecules you need by their molecular weight.
@yashfaasif938
@yashfaasif938 4 жыл бұрын
Now I understood better reading book is useless after watching this animation it's clear my all concept Thanks for making this Video
@umknownchannel7244
@umknownchannel7244 3 жыл бұрын
which books are you learning for bsc zoology 1st year
@prabhathdeep.m9836
@prabhathdeep.m9836 4 жыл бұрын
GEL ELECTROPHORESIS is really easy to understand after seeing this video Thanks
@TheWaleedKhalid
@TheWaleedKhalid 5 жыл бұрын
Great video. It helped me to better understand the concept of Gel Electroforesis. Thanks ❤️
@nomanshah2491
@nomanshah2491 2 жыл бұрын
Great, I have used same method in my research , it's very sample and very beneficial video, Thanks ♥️
@user-bi8uh5fq3q
@user-bi8uh5fq3q 4 жыл бұрын
Thank you for showing the entire procedure , now I understood electrophoresis properly after seeing all the steps... reading it from the book doesn't clear the entire concept of it.
@tessemabibo7381
@tessemabibo7381 6 жыл бұрын
it is nice.I got enough understand about laboratory session as biotechnology student and thank you!
@Hurrdurrderpable
@Hurrdurrderpable 4 жыл бұрын
Protips. Use thin wells for sharp bands. Use black paper to ease loading. You need only 1 tip if you wash ur tip in the buffer
@y.t.7832
@y.t.7832 4 жыл бұрын
شكراً جزيلاً thank you very much
@nextagro
@nextagro 4 жыл бұрын
Not sure is the comments are still answered, but... Why do we need a PLATINUM wire electrode for the electrophoresis? What exactly will happen if one replaces this to an ordinary Copper wire electrode?
@sathisht8596
@sathisht8596 3 жыл бұрын
The addition of Ethidium Bromide before the solidification of agarose gel was missing.
@Sciencemm
@Sciencemm 3 жыл бұрын
you forget to add Ethidium Bromide to the conical flask after heating the agarose solution.
@ziaullah4916
@ziaullah4916 3 жыл бұрын
A bundle of thanks
@Gauldame
@Gauldame 5 ай бұрын
The rubber seals? Bro...they come with seals? We've always had to do the blue tape walls. (when you don't know if the tray actually came with one and they were lost or if you never had them to begin with.)
@bhushanbhadade9064
@bhushanbhadade9064 4 жыл бұрын
Can we use 1X TBE buffer in to the tank
@urvashilomas6504
@urvashilomas6504 9 ай бұрын
why etbr or any other visualization dye is not added?
@iqramalik6031
@iqramalik6031 4 жыл бұрын
In DNA fingerprinting! DNA from gel is transferred to......... For hybridization?
@supershuckel4298
@supershuckel4298 5 жыл бұрын
But longer molecules of DNA will have more -ve charge then why it is behind shorter ones
@preethathilakan5402
@preethathilakan5402 6 жыл бұрын
How can we see the DNA without exposing it to UV radiation???
@adi6899
@adi6899 4 жыл бұрын
for that your going to need a microscope
@cynthiarao8324
@cynthiarao8324 4 жыл бұрын
U can't see DNA in visible light
@ambikaheerekar2158
@ambikaheerekar2158 3 жыл бұрын
How about addition of ethidium bromide???
@divyar8063
@divyar8063 3 жыл бұрын
Yeah, I think they forgot to mention about it in the video..
@mr.musical1853
@mr.musical1853 3 жыл бұрын
Amazing. Thank you 🙏
@tony232cool
@tony232cool 2 жыл бұрын
why do you need to separate dna by charge after pcr? my boss says pcr can tell us everything that is what they use in forensic files.
@Nityashah492
@Nityashah492 2 жыл бұрын
Why don't you mix agarose with etbr
@suvojitmistry4826
@suvojitmistry4826 Ай бұрын
Very well explained
@NourishHub-zh5lv
@NourishHub-zh5lv 11 ай бұрын
Thank you! it is a helpful video for my experiment.
@emmanuelgkigkilinis9124
@emmanuelgkigkilinis9124 7 жыл бұрын
Very intersting and helpful video! Quick misiterpretation question : In 5:45 you say to add 10 micro-L of control sample (DNA ladder) and then 20 μL of each of the other DNA samples, am I correct?
@preethathilakan5402
@preethathilakan5402 6 жыл бұрын
You didnt show elution.
@prachetaghosh2308
@prachetaghosh2308 6 жыл бұрын
Amazing video Thank you☺I hope that can also do the same in future days✌
@supershuckel4298
@supershuckel4298 5 жыл бұрын
So u was a neet aspirant
@mjchronicles1438
@mjchronicles1438 Жыл бұрын
Great video I think I will pass my lab test
@prathibhajay845
@prathibhajay845 2 жыл бұрын
Thank you so much❤... Plz continue to do more videos like this
@georgeieboy3875
@georgeieboy3875 3 жыл бұрын
this method i trust because you can watch it develop and prove it visually. i don't trust people saying you can hand carry a sequencer and plug it into a laptop. that doesn;t make any sense.
@Getgot-en1kv
@Getgot-en1kv Жыл бұрын
May I use QG buffer in agarose gel instead of TBE buffer?
@khansaamin8620
@khansaamin8620 3 жыл бұрын
Best way❣️
@soummi1
@soummi1 8 жыл бұрын
How can we see the DNA without the EtBr i.e intercalating agent which was not being added in the Agarose gel ?
@TheScharda
@TheScharda 7 жыл бұрын
Indeed . Thatswhat i was thinking too . We used Gelred instead of EtBr.
@kartikeyvishnoi2978
@kartikeyvishnoi2978 7 жыл бұрын
Nivedita Mitra he did say that the gel is ready to be stained. Though he did not showed the process.
@DivyaSharma-vj4vz
@DivyaSharma-vj4vz 6 жыл бұрын
Loading dye is also called ETBR
@dipanwitasarkar6285
@dipanwitasarkar6285 6 жыл бұрын
yess loading dye is also called Etbr
@LofiReverbMood121
@LofiReverbMood121 6 жыл бұрын
Loading dye ****?????
@subasubi8145
@subasubi8145 5 жыл бұрын
I`m.. biotechnology student bsc.. i do'nt know please tell any project topic..what kind of project i.. do?
@diewo3364
@diewo3364 8 жыл бұрын
hello,I want to ask that have you miss the EB or Gel Red?
@dyanakhan2011
@dyanakhan2011 5 жыл бұрын
I also want to know is it ok to skip EB?
@priyankaflorina8411
@priyankaflorina8411 7 жыл бұрын
Do they add amplified DNA sample from PCR or only DNA sample?
@thunderthunder9251
@thunderthunder9251 2 жыл бұрын
I did the same but after cutting gel to extract the DNA, the concentration was very low, dose anyone knows how to increase it?
@Woanne28
@Woanne28 Жыл бұрын
Where is the gelred or gelgreen?
@jaisramchaudhari505
@jaisramchaudhari505 6 жыл бұрын
This is helping in reading
@itspooja6181
@itspooja6181 4 жыл бұрын
Thanks a lot 😊
@renukarenu4954
@renukarenu4954 4 жыл бұрын
Superb👍👍
@ItachiAmiraLucy
@ItachiAmiraLucy 6 жыл бұрын
Won't the DNA molecules leave the walls created in the gel to swim in the TAE buffer ?
@liniencemaposa8677
@liniencemaposa8677 5 жыл бұрын
No, because they are being pulled in one direction by the current.
@tejendrarathod8351
@tejendrarathod8351 5 жыл бұрын
Great video
@Darko4323
@Darko4323 6 жыл бұрын
tips on not piercing the gel when inducing the dna?
@LofiReverbMood121
@LofiReverbMood121 6 жыл бұрын
Sir upload also video on elisa pcr chromatography centrifugation and dna fingerprinting...
@sayantansaha53
@sayantansaha53 3 жыл бұрын
Can we use TBE instead of TAE?
@ahamedtahur8357
@ahamedtahur8357 Жыл бұрын
Yup
@mandavitiwari1073
@mandavitiwari1073 5 жыл бұрын
Awesome... 😊😊😊😉
@jpjsjp4870
@jpjsjp4870 3 жыл бұрын
Thanks alot.nice explanation with video...
@prettyladyp2318
@prettyladyp2318 6 жыл бұрын
Which loading dye did you use?
@preethathilakan5402
@preethathilakan5402 6 жыл бұрын
Ethidium Bromide
@trump408
@trump408 4 жыл бұрын
@@preethathilakan5402 😲 it's too toxic... It's not suggested for use, better SYBR Green
@MatTroiLuoi
@MatTroiLuoi 7 жыл бұрын
Can I ask how many liters the microwave is ?
@zahidbhat5709
@zahidbhat5709 7 жыл бұрын
there are master mix reagents where we dont need to add tracking dye , but making gel without etbr i hv nvr seen
@omsingharjit
@omsingharjit 4 жыл бұрын
7:23 can we see it just by naked eyes ?
@arnavgandhi2942
@arnavgandhi2942 4 жыл бұрын
No, u need UV light
@omsingharjit
@omsingharjit 4 жыл бұрын
@@arnavgandhi2942 is dna Fluorescence ?
@dyanakhan2011
@dyanakhan2011 5 жыл бұрын
Can we use 2 micro- L dna ladder with 5 Micro- L of pcr product/sample?
@pratimagahane5112
@pratimagahane5112 6 жыл бұрын
best vedeo sir
@IzzaKamalHaridhi
@IzzaKamalHaridhi 2 жыл бұрын
Thank you so much..
@ritikawaghmare8822
@ritikawaghmare8822 5 жыл бұрын
uh did not added etbr
@lunallena5519
@lunallena5519 4 жыл бұрын
Muchas gracias i understand very good thank You for help me.
@hassanyousaf7824
@hassanyousaf7824 7 жыл бұрын
its gud video but you did n't tell about the loading dye and tracking dye.Try to give complete concept about experiment...
@yermiamokosuli7172
@yermiamokosuli7172 7 жыл бұрын
thank you
@sheikhforid2698
@sheikhforid2698 5 жыл бұрын
Thnks..
@baapsabka7615
@baapsabka7615 4 жыл бұрын
Everywhere I go I found loads of Indians in the comment section
@areebakhan141
@areebakhan141 Жыл бұрын
That is according to ur location set in the yt settings
@meenaeghazal3155
@meenaeghazal3155 7 ай бұрын
These basterds are almost Avery where
@ujjalpatra1223
@ujjalpatra1223 6 жыл бұрын
Thanks.
@awanimillavithanachchi931
@awanimillavithanachchi931 4 жыл бұрын
if can disply the procedure by words while play the video good
@eff_rah6475
@eff_rah6475 5 жыл бұрын
Wow great
@priyaprakashu8605
@priyaprakashu8605 4 жыл бұрын
its very intresting
@maryamghuman8852
@maryamghuman8852 8 жыл бұрын
yes u miss etbr
@marsimohi6920
@marsimohi6920 5 жыл бұрын
Best to gain better knowledge about........
@priyankanagra3637
@priyankanagra3637 4 жыл бұрын
Nice👍👍
@satheesanek932
@satheesanek932 5 жыл бұрын
Good
@noname-qn1lf
@noname-qn1lf 4 жыл бұрын
What happens when we eat a DNA
@karimabdelbary1898
@karimabdelbary1898 5 жыл бұрын
شكرا
@الهاكرالجزائري-ح5ح
@الهاكرالجزائري-ح5ح 5 жыл бұрын
كنت اظن نفسي الوحيد هنا الذي يتكلم اللغة العربية
@lavadibo3987
@lavadibo3987 3 жыл бұрын
@@الهاكرالجزائري-ح5ح نو لست لوحدك
@sagarsagar3397
@sagarsagar3397 5 жыл бұрын
Thanks
@awatifalahmad6024
@awatifalahmad6024 4 жыл бұрын
اريد باللغه العربيه نفس التجربه
@abed0019
@abed0019 4 жыл бұрын
You forget put ethidiom bromaid in liqued gel befor rins it
@princetonseparations7241
@princetonseparations7241 5 жыл бұрын
Hey all! If anyone's interested, check out our channel to see Electro-Sep - a product that can capture DNA bands in an agarose gel!
@dhruvitvadher6955
@dhruvitvadher6955 4 жыл бұрын
thank you kevane laayak nathi tu
@Biswajit_Goswami
@Biswajit_Goswami 6 жыл бұрын
Thanks.. helpful
@soumyanr2442
@soumyanr2442 2 жыл бұрын
Etbr?😶
@Mircovoice
@Mircovoice 4 жыл бұрын
I think she broke the walls in the gell
@vaibhavmhaske3480
@vaibhavmhaske3480 6 жыл бұрын
Thanks a lot
@arresteddevelopment2158
@arresteddevelopment2158 3 жыл бұрын
Why do I always say Methyl A instead of Poly A tail? Sheesh
@animeenjoyer9382
@animeenjoyer9382 5 жыл бұрын
THANK YOU
@ristusjeesus
@ristusjeesus 5 жыл бұрын
you are so cute and pcr goes firmly
@jameelgee1859
@jameelgee1859 4 жыл бұрын
Plz explain it in Urdu
@williambdagoseh541
@williambdagoseh541 6 жыл бұрын
Barkai wayy
@vaishnavipawar1603
@vaishnavipawar1603 5 жыл бұрын
Try muting the video ☕
@jsvclubdeciencia6283
@jsvclubdeciencia6283 3 жыл бұрын
In silico: kzbin.info/www/bejne/eJy3g4yDn96UqqM
@MannansElearning2021
@MannansElearning2021 3 жыл бұрын
kzbin.info/www/bejne/fKeQp5SvppqAeaM (Watch this video for precautions)
@puneethnagaraj796
@puneethnagaraj796 3 жыл бұрын
I'm from mit
@cansunuryaylacoglu6272
@cansunuryaylacoglu6272 5 жыл бұрын
Pls add Turkish language
@kichu2555
@kichu2555 3 жыл бұрын
Any class 12 Bois
@lincolnoliveira3041
@lincolnoliveira3041 3 жыл бұрын
Rmddu
@skilvarajput3151
@skilvarajput3151 7 жыл бұрын
average
@tessemabibo7381
@tessemabibo7381 6 жыл бұрын
it is nice.I got enough understand about laboratory session as biotechnology student and thank you!
@vinayvinni6827
@vinayvinni6827 5 жыл бұрын
R u BSC student
@asclepius-thegodofmedicine3810
@asclepius-thegodofmedicine3810 5 жыл бұрын
Thanks
@jaydenmweshilonga463
@jaydenmweshilonga463 Жыл бұрын
Thanks so much
@nisrinejarmoune6415
@nisrinejarmoune6415 2 жыл бұрын
Thank you
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