Thank you for the simplified lesson. Keep up the good work.
@BioLabCollective10 ай бұрын
Thanks for watching and commenting!
@luissantos23552 жыл бұрын
the level of production is amazing. how do you not have more views?
@BioLabCollective2 жыл бұрын
The algorithm is tricky to figure out, but glad you were able to find the channel!
@emmaportnoyАй бұрын
What amount of DNA should be loaded to each well?
@fatcammal7 ай бұрын
This is THE most thorough and effective PCR video i've ever seen
@BioLabCollective7 ай бұрын
Thank you so much! Glad it helps
@ronitdey80995 ай бұрын
This is exactly what I was looking for! Thank you sir for this masterpiece!!
@BioLabCollective5 ай бұрын
Thanks for watching!
@freespirit68553 ай бұрын
Do we need serial dilution for running a PCR?
@BioLabCollective3 ай бұрын
Not always, depends on what you are trying to dilute out? Same principles apply when you are setting up your PCR mastermix and diluting it out for the different reactions.
@skazka3789 Жыл бұрын
Hello again Professor! With regards to the PCR, the machine says there is an amplification in my negative controls. But the CT values for the negative controls are consistently 10 points higher than the CT values for the actual samples. For example CT value of 37 for negative control vs CT of 21 for the sample. These results are still fine yeah?
@BioLabCollective Жыл бұрын
I'm not really sure about your experimental context so can't really say by CT values alone. Have you run the samples on a gel to assess the size of the bands? Probably the next thing to try
@emmadegroote4014 Жыл бұрын
Thank you, this helped sooo much!!! You're videos are amazing!
@BioLabCollective Жыл бұрын
Glad they help - thank you for taking the time to comment!
@siasmith67463 ай бұрын
this is the clearest, most precise video I have seen on PCR. I normally do not comment but this was phenomenal
@siasmith67463 ай бұрын
I am a subscriber now, all of your videos are made with such care.
@BioLabCollective3 ай бұрын
Thank you! Took a long time to make, glad the effort shows on screen
@InquilineKea Жыл бұрын
OH THE COMB CREATES HOLES IN THE GEL
@BioLabCollective Жыл бұрын
The gel lanes (or holes) need to be standard sizing all the way across - a comb makes that happen more or less