Isoelectric Focusing and Isoelectric Point

[@Wandelaarke]

The interwebz respects people like you, who actually manage to make things clear in a clear way. Go Humanity! Thanks

[@leonardozacharias9723]

Congratulations on your incredible knowledge, fluency and dedication to the task of disseminating the biochemistry.

[@lamdinh6847]

AK LECTURES ! You are the best lecturer on youtube. To be honest , You are even better than my prof. in my school. I wish I could only stay home, eat, sleep, and watch your video for my education haha JK!. I know I am so lazy for not going to school someday, if that such a case, you are my savior.

[@tal8871]

You can do just that now! It's quarantine

[@sarahhardy4991]

This comment aged well, lmao. Now we're all doing that!

[@kameshcruise]

There's no one in the world who disagree to that 🔥🔥🔥

[@Oioioiiioioiioio]

I've been watching your videos recently, it is clear and easy to understand eventhough I am not a native english speaker. thank you for the videos, and it really helped me through the mid term test!

[@o0lale0o]

oh my goodness! I am really impressed by the quality of your teaching skills! Thank you loads; You helped me to finally understand what my biochemistry prof can't bring across in lectures at uni! You saved my good mark

[@emilygreen2436]

you're such an amazing guy. thank you for all your work!

[@AKLECTURES]

thanks Emily! I like the piano pieces! talented :)

[@emilygreen2436]

wow thanks! you are one of the only people to have heard those pieces.. not many people know about them. funny you listened. thank you so much! your work has helped me tremendously and i am so grateful.

[@AKLECTURES]

Well it was a pleasure to listen to, especially "Noch". Something about it just made it so relaxing and peaceful. Did you compose it?

[@emilygreen2436]

yah i did. i'm surprised you like it.. that's so nice. i have a few more pieces, but haven't recorded them because i've been locked up studying for the mcat. wouldn't have been able to make it this far without you actually. so thank you again. it's really nice to know someone likes my music.

[@aliaub75]

Seriously...why are u less than 1M subs? Ur a genius my friend.

[@rimaraheem1504]

I don’t usually comment on KZbin videos, but thank you so much- this was really helpful!

[@izzat7458]

The best explanation EVER! Thank you :)

[@kareem9102]

Finally I do understand the Ip. Thank you very much for amazing explanation.

[@dl1643]

OMG you're the best lecturer!!!! Thank you so much for making these videos!

[@tiredcat6653]

You are so amazing. Thank you so much.I love you.

[@alejandravalenzuela342]

I love this. Thank you for taking time for doing this!

[@faryaarshad9846]

YOU ARE THE BEST!!!! OMG I CANNOT THANK YOU ENOUGH.

[@greciaalvarez6604]

I’m teaching myself biochem bc my profesor sucks. Thanks for your lectures this is helping me too ;)

[@youknowitis]

Great Explanation 👍👍 AK

[@arinsadeghi3155]

Great video, thanks a lot. You are a great teacher!

[@Guacamole44479]

For case #2, best NOT to memorize but rather understand. Fully protonate at first (i.e., low pH) and then work your way up.

[@fatimasandoval9133]

In this lecture, when you are explaining the technique of Isoelectric Focusing, you mention that a protein with a positive net charge would move towards a higher pH. This confuses me because I have understood, and correct me if I am wrong, that if the protein has a positive net charge then it has a "surplus" of H+ atoms, therefore, I would assume that the protein is acidic. I appreciate any explanation or clarification! Thanks a lot!

[@LauraGonzalez-le8iq]

thank you for existing... really

[@Eli_YourMajesty]

Thank you so much! I love your videos and the way you teach:)

[@mahya8450]

Awesome explanation 😍🤩

[@ihsanulhaq8602]

Amazing teaching skills keep it up thanks👍👍👍

[@johnbarba851]

Excellence as always!!!! Much Appreciation !

[@snehagupta3992]

thank you so much sir for explaining such complex topics so easily...

[@adent94]

This is very helpful! I wish you were my biochemistry professor 😩😩😩😩😩

[@sungkimm123]

I wish you were my mentor!

[@mihip619]

For the tyrosine, isn't the charge on the hydroxy group supposed to be OH not O- since the pka is higher than the pH?

[@sabienblewanus4228]

If group 4 only contains Cysteine and Tyrosine, where do Serine, Threonine, Aspargine and Glutamine belong? In which of the 4 groups? Thanks! :)

[@hinsadaba2757]

I understand PI separation methods thank u for best explanation !!!!

[@kndhrty]

test in two hours. lets do this

[@saadrehman362]

how did you do?

[@DavidYang-et4ds]

Why would IEF gel not denature protein? Since by going to their pI, you're messing with protein's charge (which in turn mess with their ionic & H-bond).

[@arunkumars6257]

Thank you so much!

[@kb24baller9]

Is the negative end of the voltage source always connected to the basic end of the gel? Thank you :)

[@herimphubusa7454]

you're the best ever

[@xg4199]

Sorry, one question. For Aspartate (acidic side chain). Why do we only average alpha-COOH and side chain? If the pH=3.6, then, alpha-COOH will be negative (pH>pKa) , side chain will be positive (pH

[@moch.nafkhanalzamzami4840]

That's what I've been wondering too...

[@cheatunion]

The side chain won’t be positive but neutral. COOH is neutral when acidic and negative when basic.

[@kashafseducationalsite363]

best explanation... thank u..

[@Procrastinerd]

So theoretically if you placed a single unitary amino acid (the one you explained in step 1 where the pI would be 5.55) anywhere on the gel, the amino acid will migrate to its isoelectric point by the nature of the isoelectric focusing process - is this correct?

[@AlessandroNota]

Just perfect, thank you

[@yokoshiko9427]

the best lecture

[@TitaniumMau]

Very well explained - thank you!

[@AKLECTURES]

+Maria Xhako Thanks again !

[@cheyenneschilder2180]

These are really good lectures. Just one minor tip, maybe try to move less. It's distracting from the lecture. The rest of it is amazing. Helped me really well for my test.

[@IrfanAli-bs8nm]

@ak lectures please help me, how can I find diffusion coefficient of each peptide/amino acid? which formula should I need to use?

[@imane2858]

Thanks a lot

[@huijuinshak]

This is really good!

[@benheithoff9430]

great video, very helpful!

[@chukwuemekachikelu8153]

Thank you!

[@giwu2423]

awesome awesome job. thank you so much for these they're so helpful!

[@IdahoWilliams]

please expand the biochem series ....This is about where we're at for this current semester.

[@AKLECTURES]

yes, thats what I am current working on now. my website will have the complete biochem playlist so you can follow it there! good luck! :)

[@lateshsingh6740]

thank u

[@dylanhicks3212]

Correct me if I'm wrong...but I believe your tyrosine structure is missing a carbon.

[@massimiliano93.]

thanks

[@ayshwaryar7251]

How to separate two proteins with same isoelectric point?

[@anelahoxhallari2636]

i love this video... :)

[@ShivamKumar-kr6yz]

Which book u follow sir??

[@Supernova2995]

Amazing, I love you, no homo.

[@fluffyberry2222]

12:18

[@lovelyyarora6207]

nyc :) (y)

[@SomeCallMeGinger]

And what that means is... XD