annotating assembly

[@AbductedAlien-l3u]

tq so much for ths video . keep going on

[@selmanurkeskin]

i have come back to this video many times, thanks a lot !!

[@mikevandewege3007]

Thank you. For more detailed annotation instruction, see the Trinotate documentation :) github.com/Trinotate/Trinotate.github.io/blob/master/index.asciidoc

[@sheetal_soul]

Hello, Thanks for the very informative videos. I have a query.I am working with Transcriptome NGS data. from that, I have to find the isoforms sequence (transcript sequence to further designed primer and confirm their expression) for a gene of interest. can you tell me a pipeline I follow to achieve my desired outcomes? Thank you very much for your time.

[@mikevandewege3007]

Primer3 probably.

[@sheetal_soul]

@@mikevandewege3007 thank u for ur reply/ But I asked about isoform analysis.