Antibiotic Sensitivity Test by Disc diffusion method (Kirby Bauer method & Stokes method)

[@chigbataosaletin1215]

This really helps me a lot in carrying out sensitivity test in my lab

[@jamaicagamus8967]

This really helps me a lot about my individual report, thank you very much 💖

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[@TAMSUN14]

Very interesting sir..Also you did very nice performance this work. This like videos very useful for all trainers. Thanks for your support. Bye.

[@bashgen4211]

The best so far

[@shisampoudel532]

Thanks for making this video.

[@aryanhanamshet6823]

Extremely helpful, thank you so much.

[@maddelaanjaneyulu4615]

Sir this video helps me a lot thank you

[@relaxingshorts9928]

Sir u are simply superb😍

[@paballomoono]

What's the purpose of comparing the bacterial suspension with the 0.5 McFarland standard?

[@rejojacobjoseph]

www.slideshare.net/RejoJacobJoseph/antibiotic-susceptibility-test-ast-239030380

[@Thinker-gd8ck]

Thanks for sharing!

[@davidzhangpsu27]

Sir i have a question, how big should the antibiotic disc be? Thank you

[@rejojacobjoseph]

6mm plain disc (for research purpose) we r getting at 8mm in size

[@shubhadathorat3522]

How can we take reading of 2mm or 2cm fabric by this method

[@divya821]

But vollume is very slow kindly increase pls

[@Blevic18]

Which chart did you use as reference please and where can I download it?

[@salmaallah3703]

How many colonies do we need to pick ? Thanks

[@hddggfdaa]

What will be the distance between two disc in 110 mm Mueller Hinton Agar?

[@pink35day5]

Is incubation not required if bacterial suspension is prepared using MH broth, as with glucose broth or peptone water where the suspension is incubated before making a lawn

[@rejojacobjoseph]

Incubation is required after inoculation

[@benefoebenezer9835]

Wonderful. How do I get the interpretation charts for the antibiotics. Any help will be much appreciated. Thanks

[@rejojacobjoseph]

Interpretation chart u will get it from the antibiotic company site / u can enquire with the suppliers.

[@anjua3771]

How wil yuh add sample at different concentrations??

[@yasir3719]

Sir I prepare sample 10mg/ml concentration. For standard I use ofloxacin so I make the standard in same conc as test samples are?

[@rejojacobjoseph]

No need to prepare standard in same concentration.

[@afnan8610]

Thanks 👏👏👏👏✨⭐️⭐️

[@shubhadathorat3522]

Can this method work for 2cm or 2mm fabric test

[@sasikalac4874]

Why the bacterial suspension is compared to 0.5 Mc farland standard

[@rejojacobjoseph]

It helps to ensure that the number of bacteria will be within a given range to standardize microbial testing.

[@divya821]

Sir helpful thanks

[@meenageeths9730]

Sir helpfull thanks 👍

[@mdobaid4129]

In stokes method same antibiotics disc we've to use and same bacteria for test and control?

[@rejojacobjoseph]

Same bacteria for test & the control.. e.g: for the test u can use identified Hospital sample and for the control u need to use standard strain (ATCC strains).

[@sasmitadas345]

Sir is this entire procedure done inside luminar air flow

[@rejojacobjoseph]

Yes..All the works must done in the safety cabinet only.

[@mrstevens445]

Thanks for this. I however have a question. I did triplicate, so I find the average before comparing to an interpretation chart? Please help me

[@rejojacobjoseph]

Yes. U can take the average and then compare with interpretation chart. But the difference of the trip. results should be near by. if ur doing sensitivity test with ur own prepared antibiotic then u need to do triplicate otherwise no need.

[@mrstevens445]

@@rejojacobjoseph thank you

[@coffeestarsgalaxy9369]

what culture did you use sir, is it s. aureus and e. coli?

[@rejojacobjoseph]

S.aureus

[@خديجةالحويطي-ب9م]

Sorry, can you write the method here?

[@yuvrajkatkar2597]

👍👍

[@linussamuel8748]

My heart just keeps thanking you and thanking you Dr Igudia for all you have done for me, you gave me your words that you could heal any sickness or disease, thank you for permanently curing me of my Hepatitis B virus you are amazing

[@beyzasualp2225]

Why is there no Turkish translation?

[@sureshalamuri3666]

Well sir

[@nyesuraju912]

Tq sir

[@hddggfdaa]

What is the distance between the disc and the periphery?

[@sabhakailash7728]

👍👏👏👏👏