ID Laboratory Videos: Antibiotic susceptibility testing

"Antibiotic susceptibility testing", 2015.
Produced by Tufts University School of Medicine Center for Science Education (CSE) as part of the Infectious Disease high school curriculum:
sites.tufts.ed....
The Great Diseases curriculum provides a framework for students to learn high school biology in a more approachable and life-relevant context, designed to improve science engagement and health literacy.
Supported by the National Institute of Allergy and Infectious Diseases (1R25AI098781-01A1).
Written by EmilyKate McDonough and Kayle Sawyer.
Filmed and edited by EmilyKate McDonough.
Actors: EmilyKate McDonough, Revati Masilamani, Berri Jacque, and Eleanor Fleming
Lab space and materials: Andrew Camilli, Katya Heldwein, Tufts CSE
Music: "Discomutante" - A Smile for Timbuctu (alterations made) and "Air Hockey Saloon" - Chris Zabriskie.
Animation consultant: Andy Musser

[@TimidAction]

for any poor soul who needs to find the 9 mistakes Labeled the top of the agar plate rather than the bottom. Did not keep the cotton tip of the swab sterile. Did not orient the swab correctly when selecting a single bacteria colony from the stock culture plate. Did not spread the bacteria colony evenly over the agar plate to create a lawn of growth. Did not discard the swab in the waste container. Moved the disc after it fell on the agar plate. Placed a disc too close to the edge of the plate so it was not possible to measure the diameter of the zone of inhibition. Dropped a disc on the benchtop. Did not space the discs evenly on the agar plate.

[@Old_Guard2]

Thanks from the future, you are a life-saver

[@livybearful]

I love you

[@juandiegofajardohernandez5369]

@@livybearful omg, thank you! HAHA

[@afnansyifamuhammad9016]

OMG, Thanks So Much

[@vanessaestrela1050]

You saved me!!! thank you so much!

[@iqdefinition3253]

Thanks for not rushing through but putting it in simple and understandable terms. And that yellow glove scientist I found humorous though important as these are mistakes we could make but now we are alerted to be careful. thank you again and please keep helping students like us.

[@mitra2028]

Are antibiotic resistance test diagnosed by doing Urine for C/s test?

[@therubinlab]

I like the idea of including food colouring, that's a great teaching trick to show the concentration gradient! I think my students sometimes struggle with visualizing this with a verbal explanation alone.

[@nourserag5991]

WOW the experiment now for me seems so easy after watching every step of it carefully and also the brief summary in the end made it even more illustratable to one's mind .thank you

[@SavePaws_FriendlyWorld]

Best educational Concept Clarity.... Ur nature of Guiding is Excellent 👌 Now I can Study, rather than boring my Nuisance lectures

[@asifkhanrahime445]

I like your way of teaching please keep doing all these stuff about microbiology..... And if you have time please make some videos about its full details........... THANK YOU SO MUCH

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@yogeshlakshman8488]

You explained it like a movie mam It is so awesome hoping more videos Thank you so much❤️

[@user-cx5in7pi5c]

Heat, measurable scale, reference table, sterilization

[@SavePaws_FriendlyWorld]

U r just amazing.... crystal clear Concept. Thanks a lot madam🥰 amazing video

[@navaneeth_us]

Top-notch conceptual correlation and beautifully crafted and video. Thank you so much.

[@adammajaliwa3125]

U 're so amazing madam 🙌 u make it short but very much clear and educative too 🙏🙏

[@manaseetelaang328]

This made the concept incredibly clear, thank you!!

[@adrianamathai7481]

This was so helpful! Thank you!

[@prettysavage2203]

So beautifully addressed this lecture ❤😊

[@jenniferudeh9357]

I found this helpful ❤. I would love to connect to a medical laboratory scientist here. I am a Nigerian 😊.

[@RedRose-gw9bt]

I'm looking for a lab idea (fast) and I was wondering if E.coli bacteria was used for this lab?

[@liketylickchick]

Thank you for doing this video! I think you're an excellent teacher, also very pretty!

[@RedRose-gw9bt]

I absolutely love your channel, but what type of bacteria did you use for this lab?

[@sokefunful]

Narrator: Label the bottom of the plate Yellow gloves: how about no...

[@sunandinisingh589]

Thank you sooo much! I could not visualize this by reading from my book!

[@jessicaneamtu9053]

Thank you! This video was so helpful!!

[@jakechandler5702]

Why is she hating on scientists who wear yellow gloves? -_-

[@olduglyentwife5700]

They have messy handwriting

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@poosichomper]

I'm learning this at school, For science and I find this very interesting!

[@jericoramirez-ac5397]

What is likely to happen if antibiotic discs are placed on the inoculated media and left at room temperature for an hour before incubation?

[@aichamni3543]

I'm just a biggenner and my English is not very good but I understand this video it was so helpful thanks a lot 😘😘

[@moh19931000]

Do we have to do this in a laminar flow hood?

[@manikankana2553]

That's one engaging and informative video. Thank you.

[@yuniarelfira5793]

thanks for the video. But, i want to ask something about the preparation of inoculum. I read the protocol of Kirby-Bauer Disk Diffusion Susceptibility and it said that the preparation mus be followed by 0.5 McFarland standard. It means we have to check the density of our inoculum according to 0.5 McFarland standard. On your video, you just pick one colony from the agar then spred it with the sterile swab. According to your opinion, which one is better for the preparation of inoculum? And how the differences between them? thank you

[@divyanshumishra4802]

yeah...I am agree to you...

[@endymion_hyp]

What dye was used to show the diffusion zone?

[@unknownuser8297]

What are the 9 mistakes?

[@moonlight950]

I also want to know the 6 errors in the video

[@BrittanyTeague12398]

There are a total of 9 mistakes. If you actually watch the video, you will see the mistakes. She basically points them out for you.

[@pigeonlove]

Stooopid

[@hassanalaa2112]

@@BrittanyTeague12398 do you know what are the 9 mistakes ?? just say it briefly... p.s: i spoted 6 mistakes

[@mustafaali3333-q1m]

Ur mom

[@samassefa8579]

Great video. Super clear explanation!

[@user-ve8yt7dy6e]

هذا شرح يخبل وشغل مفصل مو مثلنا كضينا كل أسبوع تأجل الشغل عالورا 🚶‍♂️💔

[@dayanacheong901]

what is the name for the antimicrobial disc with the code W 2.5 ?

[@contentsofjoy]

Great video thanks

[@shalleey]

Thank you!❤️

[@MD-bf2ce]

I'm very new to Microbiology, but just about flipped when yellow grabbed the sterile cotton tip by the tip! Ahh!

[@aya.h8846]

What about the 9 mistakes?

[@velusamypothiraj2300]

In result they mension "sensitive" what it is means

[@nonyequeto8406]

Whats the method of inoculation? Thank u

[@Keepstudying-ex1vw]

I have one quaries ,i have isolated endophytic bacteria from plant ,then pure culture ,and i want to test my isolated strain on pathogenic bacteria by MIC Method,but i dont know how to perform it.

[@chrissychris9967]

Thank you

[@divya821]

Mam provide more like multiculture on different samples on single media

[@azizaabdumuminova3135]

Great and helpful video 👍

[@roselycullen9071]

Thank you it's quite interesting and helpful

[@tozfkfkvhv6053]

Good morning great information sister

[@erickemanueli898]

this was fabulous

[@dr.umheart2388]

Thank you so much

[@Az.Beauty.by.C]

what if a disc does not have a halo over it ? does it mean it is resistant to the antibiotic?

[@pinkeupanda6265]

yes

[@rafiaakhter2214]

what are the aseptic techniques that were used

[@shresthamukherjee6071]

So helpful 💯 Thank you so much

[@user-ch9ng3no9m]

Thank you bae 😍

[@sankimseng6094]

This so useful and help me a lot Thank!

[@zakariazaki7513]

I like this video keep going 🤠 greeting from Morocco

[@abdixakiinmukhtarcmm116]

I very interesting the way u explain thnkz

[@kmyachan9795]

Thank you very much

[@matthiaseckert7344]

Very nicely done

[@danna3922]

How to prepare the antibiotic dics?

[@gabrielrogerio7128]

muito obrigado!!

[@malakilani6283]

that was amazing, thank you!

[@daryaissa3191]

Thank u so helpful ❤️

[@ujumiriam9174]

Educative, thanks for this

[@user-tj6rf5tr7r]

Очень полезное видео, спасибо большое!

[@nurulhidayah-ls6rc]

Great and helpful.. thank you

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@mltlecturesbyTT83]

Excellent

[@anisahmad2714]

Thanks for the video!

[@naimanasrin7073]

Thank you sooo much!!!!

[@buhariabdullahi3128]

Biochemical test

[@bauhiniafolia9673]

Wait, how about the 9 mistakes?

[@kimberlycalip7981]

Same,

[@user-wz8he9lx8i]

تشکر .مرسی.tankeyou

[@idrisadamu5133]

Very interesting.

[@alakwahid]

Do you use tetracycline in different dosage for six time?? Or different antibiotics for six time?? Someone explain me

[@drhumajawed7536]

She used 6 different antibiotic, but here we can use one antibiotic with six different concentration

[@hassanalaa2112]

@@drhumajawed7536 do you know what are the 9 mistakes ??

[@pauldradre7169]

Thank you for your video

[@asifkhanrahime445]

Thank u

[@heatherwelberry1]

Why in the world would you tell us there would be 9 mistakes and then not tell us what they were so we could see if we got the right one s?? That's actually cruel, you know !!!

[@komlalebu8333]

thank you so much

[@aboodameri2145]

Thank you Soooo much

[@stellafuego]

Hi! I'm just a newbie. Just wondering how can I use garlic or other natural antibiotics for antimicrobial susceptibility test? I hope someone would answer.

[@mostafijurrahmanalhadi5292]

You need to extract the juice from garlic or other natural sources, storing them in ethanol. Make a disc soaked with those extract at different conc. (e. g. 30/60/90 microL or as you want). After preparing the discs with those natural extract, simply follow this spreading method and put your discs on the plate. Search for papers or articles on this.

[@stellafuego]

@@mostafijurrahmanalhadi5292 thanksssss

[@bsparks4211]

I like your question , impressive

[@FunnyMoments-qh6lx]

@@mostafijurrahmanalhadi5292 Men I had the same question and you answer it perfectly

[@aarani4655]

Thank you so much, really helped with my homework! :)

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@mikemiller7231]

Why are they so baffled at doctor offices when you ask for them to do a simple test like this? Compartmentalized idiots we usually call them now! We did this in high school. Now they get frustrated rude and say bs.

[@tomasescribanoselak4334]

Awesome!

[@dallonpihema16]

Thanks exam passed

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@A000a97]

@@hassanalaa2112 I found 6 mistake 1 It is mistake when picking the coloni 2 Contamination of swab cotton 3 false way to distribute 4 don't put swab cotton in recyclbin 5 antibiotic fall from forceps 6 growth of bacteria not true

[@zahraafakih7020]

To grow the bacteria no meed for a specific medium right? Just wonderning

[@45bounty]

perfect..thks so much

[@tatendachawanda6129]

Well Explained

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@sivaprasadaraoboppudi4804]

procedure explain in english

[@shalugoutam1763]

So nice

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@innocentabae1118]

Beautiful 🥰🥰

[@Peace-fo1vx]

thanks a lot

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@sam_khan_1995]

Thans

[@bilalahmad6102]

thnks ma'am.

[@naomiyorgba2420]

Pls make more video

[@krmanjahmad659]

thanks for this type of vedio i love it.

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@krmanjahmad659]

@@hassanalaa2112 @Hassan Alaa yea 1.labeling is wrong, with line writing. 2. contamination of swab. 3.wrong in striking of plate. 4. mistake in puting antibiotics in target place. 5. not handling of cup plate. 6. mistake in taking one colony. 7. forgeting small dot(.) on swab 8.upside down of plate that cause differentiation . 9. not adding another same antibiotic on plate. 10. not taking care of safety (like not putting swab in waste container).

[@humeeayisha3199]

Good job 👏

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@humeeayisha3199]

@@hassanalaa2112 no please Tell me what they are

[@bangashkpk7914]

thanks madam

[@nargizaraximova762]

It is interesting Video thanks,😇

[@axlaamali7560]

great d helpful thank you

[@zeenatullah9960]

Gr8 madom

[@kamalkaperera3599]

D diomand Test

[@khalafalasad3209]

Nice

[@hassanalaa2112]

do you know what are the 9 mistakes ??

[@jennie-jen8138]

Great video.

[@hassanalaa2112]

do you know what are the 9 mistakes ??