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Hello everyone welcome to Monu tutorial academy.
today our topic is antibiotic sensitivity test so first we will discuss about types of antibiotic sensitivity test in microbiology AND then we also study definition in easy way so you watch full video at till end. and learn everything in this Antibiotic sensitivity test video if you any doubt in this video so you ask me in comment box then I replied in shortly time. your doubt in any subject like hematology, microbiology then you tell me in comment box also.
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Microbiology playlist :-
Microbiology: • Microbiology
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Histopathology: • Histopathology
Theory of antibiotic sensitivity test :-
Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics. It is used because bacteria may have resistance to some antibiotics. Sensitivity testing results can allow a clinician to change the choice of antibiotics from empiric therapy, which is when an antibiotic is selected based on clinical suspicion about the site of an infection and common causative bacteria, to directed therapy, in which the choice of antibiotic is based on knowledge of the organism and its sensitivities.[1]
Sensitivity testing usually occurs in a medical laboratory, and may be based on culture methods that expose bacteria to antibiotics, or genetic methods that test to see if bacteria have genes that confer resistance. Culture methods often involve measuring the diameter of areas without bacterial growth, called zones of inhibition, around paper discs containing antibiotics on agar culture dishes that have been evenly inoculated with bacteria. The minimum inhibitory concentration, which is the lowest concentration of the antibiotic that stops the growth of bacteria, can be estimated from the size of the zone of inhibition.
Antibiotic susceptibility testing has occurred since the discovery of the beta-lactam antibiotic penicillin. Initial methods were phenotypic, and involved culture or dilution. The Etest, an antibiotic impregnated strip, has been available since the 1980s, and genetic methods such as polymerase chain reaction (PCR) testing have been available since the early 2000s. Research is ongoing into improving current methods by making them faster or more accurate, as well as developing new methods for testing, such as microfluidics.
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