MIC By Agar Dilution Method | Antibiotic sensitivity Test By Agar Dilution Method |

[@BMHlearning]

At 4:54 the container with red solution is actually agar, for 4 microgram antibiotic solution.... After the antibiotic is added into the agar solution.... Its poured into the petri dish ( the one in grey color...)

[@ryd3775]

I really want to understand how to prepare the inoculum. I still don't know if I need to dilute the inoculum from 10ˆ8 until 10ˆ5, it means 3x diluting it 1/10 or something else.

[@khemzie3616]

nice video... good narration thank you

[@sepidehshahriary4528]

Thank you for the informative video. That would be great if you help me with the confusing part on 3:55 which is animated by transferring 800 micro liter while the mentioned transferred amount is 100 micro liter, besides considering the dilution calculation formulas the proper amount to be transferred from stock solution to make the 4 micro-gram / ml solution should be 0.08 ml or 80 micro liter.

[@BMHlearning]

It might be possible that there is a calculation mistake in the video... I dont really remember how i made the video. My suggeation is go by the formula

[@sepidehshahriary4528]

@@BMHlearning Thank you very much for your repl. Any good text book to suggest for calculating different antibiotic stock solution dilution to perform MIC ( Broth/Agar)test ?

[@rossulh.al-bahadili3340]

I have a question at 5:00, if add 50ul of the antibiotic at 4 ug/ml to 50 ml agar, does that change the concentration of antibiotic?

[@muhammadabdullah2735]

Appreciated thank you 😊

[@brajesdoez1282]

Bro one more doubt. What is the difference between the red coloured solution and gray coloured solution at time 5:04. Because both of the solution is labeled with the same ' 4ug/ml antibiotic sol' .

[@BMHlearning]

At 4:54 the container with red solution is actually agar, for 4 microgram antibiotic solution.... After the antibiotic is added into the agar solution.... Its poured into the petri dish ( the one u said as grey... I drew the petri in that color)

[@brajesdoez1282]

@@BMHlearning Thanks bro for explaining. Now I have understood

[@dhinakaranirusan5130]

One dilution how many plates used 4 micro gram 1 tube

[@brajesdoez1282]

Super video bro. Bro I have a doubt on how you got the concentration as 4ug/ml at time 4:07. I get 40ug/ml. Can you explain?

[@BMHlearning]

Thanx for that.... Actually u are right... Its 40 not 4.... I miscalculated it.

[@brajesdoez1282]

@@BMHlearning Thanks bro for explaining. Now I have understood

[@Vijayhemmadiprakash]

@@BMHlearning exactly

[@abdulrehman6643]

​@@brajesdoez1282 can you explain me what is the formula?

[@زمنصاحب-غ3ف]

Dr. When I isolated bacteria from food sample can I do Susceptility test and I read result aCorredin clsi M 100

[@umaimakhan7974]

At 4:12 how you make 4 micrograms per 1 ml from 1 mg per ml?

[@pomelobunnycarrot9153]

It's nice video, I have a question at the time 5:00. How many volume of gray solution that you add in red solution? How many volume of red solution that you pour in a plate? I communicate English language not well, I'm so sorry but I hope to get answer from you, Bro. Thank you.

[@BMHlearning]

Have a look at the pinned comment.

[@shameemamol5315]

Sir I want the MIC range from 8 to 4..can u please help me with the calculation

[@BMHlearning]

Its calculation part, just reduce the number as u want

[@kaitlyn7171]

at 5:00 you did not say the volume of the agar you are using or the volume of the solution that is being added.

[@BMHlearning]

May be ...

[@laxmeshwarpharmacy8718]

How is it become 4 micro per ml , 1 mg / ml added in 19.2 ml to obtained 5 micro gram / ml

[@BMHlearning]

There is some miscalculation in the video .....

[@md.zahirulislam9379]

Anyone have written pdf of this method ???