Antimicrobial activity of Plants/Clearing zone/Disc and well method/Medicinal plants/Microbiology

[@avocadoexplanations7582]

I had so many doubts about all these things (as i am currently working on antimicrobial activity)................. Thankyou sooo sooo much for clearing my doubts.❤....... You're a life saver!

[@anshikagarg1644]

For plates incubation at which temperature ???

[@Botanyphd]

Thank you so much sir ❤ Well explained... You are a blessing to research scholars or Ph.D. students.. 🙏🙏

[@Dr.Rakesh.B]

Means a lot 🙏

[@claudinec.padalapat1891]

Thanks Sir, Now I have a good guide for our research paper.

[@Botanist-ib6qd]

Thanks for clearing all doubts

[@whitetulips1972]

U r doing great job sir .. but please also demonstrate the statistical analysis of the obatained data.. it would be much helpfull

[@Dr.Rakesh.B]

Sure

[@madhumithaganesh4560]

@@Dr.Rakesh.B sir I’m doing a research on this same topic, in MBBS, kindly guide me in statistics and proposal

[@vikasg4683]

Amazing sir. All things well explained. Thanks a lot sir. Keep it up. #Please wear hand glows while performing practical.

[@ericwuluglayjr6060]

Can you pls explain on how crude extracts can be used to test antibacterial and antifungal activities. Considering the the following: concentration, pH, temperature etc.🙏🌷

[@Dr.Rakesh.B]

You can use different solvents to extract your compounds. And also can check their pH. Preferably a weak acid, neutral or strong acid solvents you can use to check their growth . Also take in different concentrations. For temperature you can do in two ways, you can alter the extraction temperature or incubation temp.

[@aamiraamir499]

Sir how to dissolve plant extract completely because I have crude for of Chinese rice flower plant but when I dissolve in water it's not completely dissolve please suggest me which chemical I use to completely dissolve it

[@Dr.Rakesh.B]

Read paper on that plant as i have not worked with it

[@anupamamoirangthem1020]

Could you kindly tell us how to calculate the concentration of the extract (in Microgram) so that we can say this much microgram is inhibiting the microbial growth

[@VARSHAPBT]

beautiful explanation sir..this vedio is very helpful sir..

[@priyankajayaprakash1818]

Thankyou so much for the great explanation 👍

[@bibirazakhanam8769]

Very good explanation. Thank you.

[@Dr.Rakesh.B]

Thank you

[@bhushansong]

Very nice explanation. Thank you I have 1 query related to methanol or ethanol. Since, these solvents themselves have antimicrobial activity, do we have to prepare a blank well for them? or prepare a suspension with water by evaporating the solvent? Please clarify.

[@Dr.Rakesh.B]

Yes, solvent itself will be considered as a blank in one trial

[@uzmasubhan877]

Very well explained sir I couldn't download your research paper.... Pls help

[@Dr.Rakesh.B]

Mail me [e-mail]

[@dr.b.l.manjula6648]

Great explanation Rakesh...

[@Dr.Rakesh.B]

Thank you mam 🙏

[@creativedressdesigningidea7307]

​@@Dr.Rakesh.B sir can I get ur contact or email I'd plz I have some questions regarding my research I found ur videos really helpful but I still have questions

[@sunitachoudhary3665]

How to make 25microgram /ml solution of plant extract. 25miceogram is very less amount so how weigh this amount ?

[@nivedaponmani8945]

After loading the samples in the well and while keeping it in the incubator the petridish should be kept inverted or not?

[@vikashganvit3548]

How to calculate the R-value (antibacterial activity ). I use iso 22196 method

[@IsmailSarker-o9b]

Your website is not working how can I download this article?

[@crazyboys1103]

Thank Sir. very nice . clear all douts.

[@ak-hl1sb]

Dear sir herbal extract me jab fermentation hota hai liquid me to vo bottom me ppt bana ta hai to aise case me micro lab ko konse test karne ke liye batye bcz vo kya krte hai turbidity test krte hai but herbal me to turbidity hoti hai so please suggest

[@claudinec.padalapat1891]

Hi Sir, may I know during incubation of well diffused petri plate well it be okay if I invertedly position the plates?Will the inoculated extracts would stay in tact?

[@hksm87]

In my experience the solutions added in to the wells get absorbed and then you can invert the plates within few hours.

[@richellecoraza2029]

Hi sir! Can i ask where to message you? I need to ask something about our research. Your videos are very helpful. Thank you so much

[@Dr.Rakesh.B]

[e-mail]

[@botanika3567]

sir , I have a one question please send the which method of plant extraction is correct

[@Dr.Rakesh.B]

I have made one video for it in my channel pls watch

[@sarashamseldin9864]

A question please ... Do we add the discs immediately on the plate after inoculating the extract or do we leave them to dry first before putting them on the plate ... And thanks alot 😊

[@Dr.Rakesh.B]

You can dry them for few seconds and immediately put. The more time more chances of contamination. Moreover as you add drop wise the disc absorbs the extract so it won't be a issue

[@sarashamseldin9864]

@@Dr.Rakesh.B oh I understand ... Thank you^_^

[@LeahGergid]

Hi, Sir! Thanks for this video. If I may ask how to prepare homogenized fungal suspension? Once prepared, is it good to compare it with 0.5 McFarland Standard? I hope someone can help me with my query. Thank you in advance!

[@aartiuprikar5343]

I work on different extract of Leucas biflora leaves I want asked u que. that the extract is solidify or direct liquid is used for antimicrobial activity. Or solidify extract is dissolved in DMSO.

[@Dr.Rakesh.B]

Liquid extract is used. If it's solid redissolve in suitable solvents.

[@aartiuprikar5343]

thank you sir

[@seemasathe864]

Sir plz can you make video on Poisioned food technique for antifungal activity using plant extract and tehn how to calculate MIC .

[@fionamassey2678]

Thankyou for this video sir i wanted to ask you something I have to do antimicrobial activity of my nano particles i wanted to know when I have to load the nanoparticles solution in the agar plates during the lawn making or the next day when microbial lawn has appeared please help me with this

[@SaiSurajMulukala]

No link in the description , need the links of Killing of microbes through mechanisms

[@jijijith5819]

How can dissolve magnesium oxide nano particles ?

[@hksm87]

Best expansion ❤

[@muralibond1373]

Very informative video sir, how to make antibiotic at different concentrations..

[@haymanottewelde8427]

how to prepare diferent concentration of plant extract?

[@Dr.Rakesh.B]

Pls watch my video on plant extract preparation.

[@ritubasumatary8319]

Sir u r amazing🙏🙏🙏

[@babusheikh6652]

Dear sir, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?

[@Dr.Rakesh.B]

You should not filter the extract . Add directly.

[@babusheikh6652]

@@Dr.Rakesh.B Thanks a lot sir

[@babusheikh6652]

@@Dr.Rakesh.B But how can I convince my supervisor that this filtration step is not necessary? Can you suggest me any convincing answer sir?

[@Dr.Rakesh.B]

Just mention that there are no papers who have followed that filtration step.

[@Dr.Rakesh.B]

And even if you filter it, after filtering make up by adding the same solvent. Solvent need not be filtered since your using that as a control in a well. So make up the concentration using the same solvent

[@kubra_nik1712]

Thank you sir, yours vedio is really helpful Could you tell me how to make fungal inoculum culture is it broth or agar medium And to culture Aspergillus niger which broth i have to use

[@Dr.Rakesh.B]

You can use both to make culture. PDA is used

[@kubra_nik1712]

@@Dr.Rakesh.B thank you

[@easychemistryofficial]

Sir can we use same procedure for Determination of MIC of antifungal agent...

[@karishmawaghmare2927]

We have prepared methanolic extract so which solvent can be used to check antifungal activity? Kindly provide your suggestion sir.

[@Dr.Rakesh.B]

If you have prepared methanolic extract only methanol can be used. If you need to use different solvents again you need to prepare different extracts. Watch my video about plant extract preparation in my channel to understand.

[@sathsaradiwanjan9368]

Thank you sir, great explanation❤❤❤

[@farishtayali1892]

Ur way of guiding is very impressive and obsorbing

[@Dr.Rakesh.B]

Means a lot. Thank you :)

[@shubhadathorat3522]

Sir can you please give some idea about how to check antimicrobial activity of fabric

[@savitasandur2037]

Sir i want to lyophilize my isolates mat, and also for GS-MS, HPLC, FTRI, please let me know the centers in Karnataka.

[@shalinis3738]

Thank you sir..well explained ..sir can you explain comparison between the standard drug , why we should compare ..

[@Dr.Rakesh.B]

To compare whether your extract is more powerful or commercial drugs

[@shalinis3738]

Ok sir..tq🙏🙏🙏

[@reshmidas7019]

Great explanation sir Sir i have one question For agar well diffusion, i have to pour the media then put the inoculum n spread it, or i can mix the inoculum with the PDA and then pour it ? Actually i am confused with this thing

[@soumyakvijayan6209]

Nicely explained Sir, thank you.I have a doubt that,for microbial activity ,do we need to measure the diameter or radius of clear zone?

[@Dr.Rakesh.B]

Radius

[@husainagandhi9922]

thank you sir this video was really helpfull but i require more knwledge related to this because i m performing my dissertation on turmeric antimicrobial affcts on bacteria which harm the fish so if i can get any help via you would b appreciated

[@abdurrahmanmahir4969]

Please sir what is the difference between Antibacterial and antitumor activity. My research topic is Antitumor activity of plant extract using Agrobacterium tume Thanks

[@Dr.Rakesh.B]

Antibacterial is how efficiently extracts kill bacteria, antitumor is how efficiently tumor/cancer cells are killed by plant extract

[@Artist-z4e]

Sir..what is the measurement of microbial inoculum, which added to the plate

[@Dr.Rakesh.B]

Depends on the well size 10-50 ul

[@shubhadathorat3522]

Can you give me some idea about taking antibacterial activity of fabric

[@neyamurugesan9128]

Can I use this method with plant oils?

[@Dr.Rakesh.B]

Yes.

[@brijeshkumari6501]

Bhaut ache se samjaya thanks sir

[@ankitayadav2404]

very helpful thankyu so much sir

[@hiteshisabharwal4929]

Helo Sir, Can you tell me how to make the fungal spore solution from the spores?

[@Dr.Rakesh.B]

Im sorry i haven't done that.

[@nielvincansejo]

How can positive control be prepared? Should we dissolve it into a certain amount of water? TIA

[@Dr.Rakesh.B]

Yes

[@nielvincansejo]

@@Dr.Rakesh.B Can you suggest, Sir, such a published article on the preparation of a positive control? Thank you so much!

[@nielvincansejo]

May I also ask this one, Sir. Can we use Whatman No.1 filter paper as an alternative for the antibiotic assay disc? If possible, does it affect the ZOI of the assay solution? Thank you so much!

[@Dr.Rakesh.B]

I don't think so in papers such details for positive control will be given. Anyway u can browse them. If you're using well method directly you can pipette the samples control into well. Disc method requires the disc as it has a known conc. of equally coated drugs which cannot be compensated by filter papers.

[@KV.Organics]

Dear sir, how can I contact you?

[@ressurection7278]

Sir which solvent should I use for dissolving silver nanoparticles.

[@Dr.Rakesh.B]

Water, check my papers for more information from research gate

[@ressurection7278]

I did try dmso 10 percent but it doesn't dissolves.

[@Dr.Rakesh.B]

@@ressurection7278 they won't dissolve very easily. Read papers on your work to know how to dissolve

[@Skincare179]

Please make a video on extract preparation of latex,(dry latex method) since i am finding it very difficult to work on.

[@Dr.Rakesh.B]

Will check.

[@dr.vilaschaudhari]

where can i find the disc for disc diffusion method in India?

[@Dr.Rakesh.B]

Order from merchants who sell scientific products. Google it you will find...or order from Himedia

[@tt-ys3zq]

Did u use whatman filter paper 1 for making disc with punching machine..pl tell..

[@Dr.Rakesh.B]

No it's available in market our lab had that.

[@tt-ys3zq]

@@Dr.Rakesh.B can u pl tell the make

[@tt-ys3zq]

@@Dr.Rakesh.B and can whatman filter paper be used

[@Dr.Rakesh.B]

No whatmann doesn't serve the purpose. You can try as a trial and error and check. The one I used was purchased not made. So I don't know the make. You can buy it as sterile disc's from internet. Or you can use even without that by well method or broth method watch that video too.

[@tt-ys3zq]

@@Dr.Rakesh.B pl tell the manufacturer name

[@TasawarFish]

What are the findings of your research

[@TasawarFish]

Where they applied

[@therebel5378]

Sir will you please explain with MIC

[@Artist-z4e]

Sir.. What is the inoculum density... in your experiment??

[@Dr.Rakesh.B]

It was for demonstration. All those were not calculated

[@TasawarFish]

Sir what is the importance of this reasearch to Pakistan and whole world

[@AlokDas-yj3xb]

sir please share your paper, the link is expired.

[@Dr.Rakesh.B]

Mail me ill send [e-mail]

[@nurhidayahbazir7716]

Sir can you explain me about "antimicrobial activity of zno at different ph values" ?? How to prepare this?

[@radhikagola2208]

How can we prepare spore culture of aspergillus?

[@Dr.Rakesh.B]

Im sorry I haven't performed that.

[@VARSHAPBT]

where I get sterile disc sir..for my pg research work I want sterile disc sir..where I get it sir..help me sir..

[@Dr.Rakesh.B]

Order online from Himedia

[@VARSHAPBT]

thank you sir

[@sunitachoudhary3665]

Sir please explain me . What is the control during antibacterial activity .

[@Dr.Rakesh.B]

Just the solvent with which you used to prepare the extract. In my Channel 2 videos are there for antimicrobial activities watch both you will understand better

[@sunitachoudhary3665]

@@Dr.Rakesh.B ok . Thanku sir

[@TasawarFish]

What are the main terms of your research

[@shrutipandey3127]

Very nice

[@jankies007]

Hi I have two questions related to the topic (+ I just want to say thank you so much, these videos are exceedingly helpful) : 1. if I am testing an antifungal activity of a substance (garbage enzyme from citrus peels to be specific), can I use well diffusion method? 2. what is the most reccomended method for inoculating the fungi? Is it the same method shown in the video (using liquid spore culture first, then the liquid is spread into the plates), or can I simply transfer a portion of a culture inoculated in a plate using a scalpel? Or is there other beter method? I am using Botrytis cinerea as the fungi btw. So it's antifungal activity of garbage enzyme from citrus peel againts Botrytis cinerea From what I can understand so far (by researching the topic on the internet), the standardized/more reccomended method is using broth dillution, but I am also under the impression that well diffusion can also work to test antifungal activity againts Botrytis cinerea from a study I had found titled "A Comparative Study of Antimicrobial and Antioxidant Activities of Plant Essential Oils and Extracts as Candidate Ingredients for Edible Coatings to Control Decay in ‘Wonderful’ Pomegranate" Sidenote : tysm for the content you post here, as a student I find them very useful. It is perfectly understandable if you dont find the time to respond to my very specific questions, and sorry for taking your time

[@aimananwar7507]

For which extract( methanol or ethanol) we use DMSO chemical??

[@Dr.Rakesh.B]

Dmso is a solvent. Like water , methanol ethanol it is also useful to make extracts.

[@aimananwar7507]

@@Dr.Rakesh.B actually I made ethanol extract and Wan to find its effect on bacteria so ethanol can't be used so I dried it and now I want to dissolve it in dmso so can you tell me how much amout should I use for dried weight of extract

[@Dr.Rakesh.B]

Concentration you should decide 1 mg/ ml or 10 mg/ml like this based on review of literature

[@aimananwar7507]

@@Dr.Rakesh.B okay thanks😇

[@AnjaliSingh-ug7il]

What do u mean use methanol as control do I use methanol alone on a dic and place it on the media ?? I'm sorry I'm a first sem student I have doubts

[@hksm87]

You can use different positive controls such as antibiotics

[@sruthyepmthrissur7459]

Could you please tell the references of this antibacterial study

[@sekharsbiofactory8445]

Great job sir

[@lrastina2496]

Can we use sterile filter paper disc ?? I dont gave sterile disc

[@hksm87]

Sure. Make the disks and the autoclave them.

[@sarram.belili8087]

Thank you for the video :) I have some questions please: - you are testing an antifungal activity so why are you using an antibiotic as a control? - and, can you please tell me how to prepare plant extract dilutions and what diluent to use?

[@Dr.Rakesh.B]

Yes you are right, It was just for demonstration to show the audience. I haven't used any standard antimicrobial drugs here. For dilutions its trial and error, refer papers. To make plant extracts u can watch my video in channel

[@bety3017]

@@Dr.Rakesh.B plz sir can you expline how to make. Different concentrations of extrait

[@Dr.Rakesh.B]

Watch my video on methanolic plant extract preparation in my channel. There I have explained.

[@iznaahere]

For comparison u used antibiotic disc with what kind of disc? Like i don't get it. Was it not the antibiotic disc in which you added the extract drop wise previously?

[@Dr.Rakesh.B]

No they are just plain discs which will absorb ur material or samples. You get antibiotic coated disc which we use as std. If you don't get that you can buy a antibiotic from chemist and dissolve it and use that

[@iznaahere]

@@Dr.Rakesh.B okay so i just knew about the antibiotic discs. Now i came to know that we can find simple discs as well. I got it now. Thanks alot for the guidance. Stay immensely blessed Sir! 💫

[@nahushshekar.rnahushshekar2535]

sir is there any method for anti diabetic activity using microbes

[@Dr.Rakesh.B]

I have not done anything related to it. I'm sorry I don't know

[@Artist-z4e]

Triplicate sample testing.......Is this essential for these antimicrobial assay???

[@Dr.Rakesh.B]

Yes they are to calculate error

[@vennilaphysics8740]

Super explain

[@pavs7827]

Sir kindly make a vedio on flagella staining sir...

[@Dr.Rakesh.B]

Ok sure :)

[@pavs7827]

@@Dr.Rakesh.B I'll b waiting for that vedio sir....kindly....

[@farishtayali1892]

Plz share antihemolytic, phytotoxicity and cytotoxicity assay ... Plzzzz

[@Dr.Rakesh.B]

Ok

[@Thisara_Me]

Thank you so much ! 👏👏

[@madarajayanetti5357]

How to determine the concentration of the microbial culture?

[@Dr.Rakesh.B]

Its based on volume thats all. Not quantitative

[@fatimaanjum6984]

Thank you 😊

[@unnatirathod8098]

Very useful

[@Dr.Rakesh.B]

Thank you :)

[@aabsheensaba6532]

How we make pour culture?

[@Dr.Rakesh.B]

Using micropipette

[@dianahaobam8536]

Thank u very much sirrrr🙏

[@deepagupta919]

Sir how to prepare aloe vera plant extract

[@Dr.Rakesh.B]

Please do watch the video on plant extract preparation in my channel

[@navedanjum748]

From where to get antibiotic

[@Dr.Rakesh.B]

You can take from medical shops/pharmacy. If you want more pure forms or discs you have to order them through companies which sell chemicals

[@biryani6861]

sir how if we have sample venom, which media will be better

[@Dr.Rakesh.B]

Venom?

[@biryani6861]

@@Dr.Rakesh.B sir actually I'm using the venom of cobra in order to find bioactive peptides that can use for antibacterial activity

[@Dr.Rakesh.B]

Ok, then venom is usually too toxic and concentrated. You can dilute it with water. Say for eg. 50ul in 450ul water and consider this as your stock. From this pipette different concentrations may be 25, 50, 75, 100 or 50, 100, 150, 200 etc

[@biryani6861]

@@Dr.Rakesh.B Alright sir, thank you very much, sir i have another question at what ic50 value sample will be consider potent against bacteria

[@Dr.Rakesh.B]

That value varies from sample to sample. But generally lower the value higher the significance

[@upscziddi4228]

Thanku sir for video

[@naitikpatel6164]

How to join you for work sir I want to work with you.

[@Dr.Rakesh.B]

I finished my PhD I'm teaching for NEET bio now.

[@naitikpatel6164]

@@Dr.Rakesh.B i am doing MSC MICROBIOLOGY i am searching some prectical methods for my research and i found your video and it have so much information about my work. So i wants to join you for work so i gain more knowledge about my fild.

[@Dr.Rakesh.B]

I don't know of I can be of any help Mail me at [e-mail]

[@chathuminidias9863]

Thank you !!!!

[@sanidas6627]

Sir how to prepare plant extract ?

[@Dr.Rakesh.B]

Follow this method kzbin.info/www/bejne/rGLHeaCIjNWZeJY

[@swapnasg5985]

Sir ,I want to research in antibacterial activity area.....can u please suggest me topic ....actually I done M.Sc in Physics

[@Dr.Rakesh.B]

You got to read lots of papers to find the gap. It's not so easy for me to suggest you and I'm not that experienced too

[@nehabinwal1089]

Sir is this method is useful for measuring antifungal activity also, i mean in disc diffusion method and well method fungal mycelium is placed in centre of the petridisc or spread all over please reply. I have a confusion Whether to put a fungal mycelium disc in the centre of the agar plate or to spread it

[@Dr.Rakesh.B]

Yes you can use for fungal. What i did is fungus only

[@nehabinwal1089]

@@Dr.Rakesh.B sir i have to put fungal mycelium disc at centre??

[@TasawarFish]

Why you use this topic