I had so many doubts about all these things (as i am currently working on antimicrobial activity)................. Thankyou sooo sooo much for clearing my doubts.❤....... You're a life saver!
@anshikagarg16443 ай бұрын
For plates incubation at which temperature ???
@Botanyphd Жыл бұрын
Thank you so much sir ❤ Well explained... You are a blessing to research scholars or Ph.D. students.. 🙏🙏
@Dr.Rakesh.B Жыл бұрын
Means a lot 🙏
@claudinec.padalapat18915 ай бұрын
Thanks Sir, Now I have a good guide for our research paper.
@Botanist-ib6qd21 күн бұрын
Thanks for clearing all doubts
@whitetulips19723 жыл бұрын
U r doing great job sir .. but please also demonstrate the statistical analysis of the obatained data.. it would be much helpfull
@Dr.Rakesh.B3 жыл бұрын
Sure
@madhumithaganesh45602 жыл бұрын
@@Dr.Rakesh.B sir I’m doing a research on this same topic, in MBBS, kindly guide me in statistics and proposal
@vikasg46833 жыл бұрын
Amazing sir. All things well explained. Thanks a lot sir. Keep it up. #Please wear hand glows while performing practical.
@ericwuluglayjr60602 жыл бұрын
Can you pls explain on how crude extracts can be used to test antibacterial and antifungal activities. Considering the the following: concentration, pH, temperature etc.🙏🌷
@Dr.Rakesh.B2 жыл бұрын
You can use different solvents to extract your compounds. And also can check their pH. Preferably a weak acid, neutral or strong acid solvents you can use to check their growth . Also take in different concentrations. For temperature you can do in two ways, you can alter the extraction temperature or incubation temp.
@aamiraamir4992 жыл бұрын
Sir how to dissolve plant extract completely because I have crude for of Chinese rice flower plant but when I dissolve in water it's not completely dissolve please suggest me which chemical I use to completely dissolve it
@Dr.Rakesh.B2 жыл бұрын
Read paper on that plant as i have not worked with it
@anupamamoirangthem1020 Жыл бұрын
Could you kindly tell us how to calculate the concentration of the extract (in Microgram) so that we can say this much microgram is inhibiting the microbial growth
@VARSHAPBT Жыл бұрын
beautiful explanation sir..this vedio is very helpful sir..
@priyankajayaprakash18182 жыл бұрын
Thankyou so much for the great explanation 👍
@bibirazakhanam87692 жыл бұрын
Very good explanation. Thank you.
@Dr.Rakesh.B2 жыл бұрын
Thank you
@bhushansong Жыл бұрын
Very nice explanation. Thank you I have 1 query related to methanol or ethanol. Since, these solvents themselves have antimicrobial activity, do we have to prepare a blank well for them? or prepare a suspension with water by evaporating the solvent? Please clarify.
@Dr.Rakesh.B Жыл бұрын
Yes, solvent itself will be considered as a blank in one trial
@uzmasubhan87716 күн бұрын
Very well explained sir I couldn't download your research paper.... Pls help
@Dr.Rakesh.B16 күн бұрын
Mail me rakeshb96@yahoo.com
@dr.b.l.manjula66483 ай бұрын
Great explanation Rakesh...
@Dr.Rakesh.B3 ай бұрын
Thank you mam 🙏
@creativedressdesigningidea73072 ай бұрын
@@Dr.Rakesh.B sir can I get ur contact or email I'd plz I have some questions regarding my research I found ur videos really helpful but I still have questions
@sunitachoudhary36652 жыл бұрын
How to make 25microgram /ml solution of plant extract. 25miceogram is very less amount so how weigh this amount ?
@nivedaponmani8945 Жыл бұрын
After loading the samples in the well and while keeping it in the incubator the petridish should be kept inverted or not?
@vikashganvit35482 жыл бұрын
How to calculate the R-value (antibacterial activity ). I use iso 22196 method
@IsmailSarker-o9b6 ай бұрын
Your website is not working how can I download this article?
@crazyboys1103 Жыл бұрын
Thank Sir. very nice . clear all douts.
@ak-hl1sb10 ай бұрын
Dear sir herbal extract me jab fermentation hota hai liquid me to vo bottom me ppt bana ta hai to aise case me micro lab ko konse test karne ke liye batye bcz vo kya krte hai turbidity test krte hai but herbal me to turbidity hoti hai so please suggest
@claudinec.padalapat18915 ай бұрын
Hi Sir, may I know during incubation of well diffused petri plate well it be okay if I invertedly position the plates?Will the inoculated extracts would stay in tact?
@hksm874 ай бұрын
In my experience the solutions added in to the wells get absorbed and then you can invert the plates within few hours.
@richellecoraza202911 ай бұрын
Hi sir! Can i ask where to message you? I need to ask something about our research. Your videos are very helpful. Thank you so much
@Dr.Rakesh.B11 ай бұрын
Rakeshb96@yahoo.com
@botanika35672 жыл бұрын
sir , I have a one question please send the which method of plant extraction is correct
@Dr.Rakesh.B2 жыл бұрын
I have made one video for it in my channel pls watch
@sarashamseldin98642 жыл бұрын
A question please ... Do we add the discs immediately on the plate after inoculating the extract or do we leave them to dry first before putting them on the plate ... And thanks alot 😊
@Dr.Rakesh.B2 жыл бұрын
You can dry them for few seconds and immediately put. The more time more chances of contamination. Moreover as you add drop wise the disc absorbs the extract so it won't be a issue
@sarashamseldin98642 жыл бұрын
@@Dr.Rakesh.B oh I understand ... Thank you^_^
@LeahGergid9 ай бұрын
Hi, Sir! Thanks for this video. If I may ask how to prepare homogenized fungal suspension? Once prepared, is it good to compare it with 0.5 McFarland Standard? I hope someone can help me with my query. Thank you in advance!
@aartiuprikar53432 жыл бұрын
I work on different extract of Leucas biflora leaves I want asked u que. that the extract is solidify or direct liquid is used for antimicrobial activity. Or solidify extract is dissolved in DMSO.
@Dr.Rakesh.B2 жыл бұрын
Liquid extract is used. If it's solid redissolve in suitable solvents.
@aartiuprikar53432 жыл бұрын
thank you sir
@seemasathe864 Жыл бұрын
Sir plz can you make video on Poisioned food technique for antifungal activity using plant extract and tehn how to calculate MIC .
@fionamassey2678 Жыл бұрын
Thankyou for this video sir i wanted to ask you something I have to do antimicrobial activity of my nano particles i wanted to know when I have to load the nanoparticles solution in the agar plates during the lawn making or the next day when microbial lawn has appeared please help me with this
@SaiSurajMulukala2 жыл бұрын
No link in the description , need the links of Killing of microbes through mechanisms
@jijijith5819 Жыл бұрын
How can dissolve magnesium oxide nano particles ?
@hksm874 ай бұрын
Best expansion ❤
@muralibond1373 Жыл бұрын
Very informative video sir, how to make antibiotic at different concentrations..
@haymanottewelde8427 Жыл бұрын
how to prepare diferent concentration of plant extract?
@Dr.Rakesh.B Жыл бұрын
Pls watch my video on plant extract preparation.
@ritubasumatary8319 Жыл бұрын
Sir u r amazing🙏🙏🙏
@babusheikh66522 жыл бұрын
Dear sir, please help! I am conducting the antibacterial assay of my plant sample following the well diffusion method. But I have faced with a problem. Should I filter my plant sample with the help of 0.2 micron filter inside laminar flow cabinet before adding the samples into the wells. I found that when I filtered my sample, the concentration as well as the volume of my prepared sample also get decreased. I have also gone through severe literatures of my field but found no paper mentioning the use of 0.2 micron filter. So is it necessary to do so or I can skip this step?
@Dr.Rakesh.B2 жыл бұрын
You should not filter the extract . Add directly.
@babusheikh66522 жыл бұрын
@@Dr.Rakesh.B Thanks a lot sir
@babusheikh66522 жыл бұрын
@@Dr.Rakesh.B But how can I convince my supervisor that this filtration step is not necessary? Can you suggest me any convincing answer sir?
@Dr.Rakesh.B2 жыл бұрын
Just mention that there are no papers who have followed that filtration step.
@Dr.Rakesh.B2 жыл бұрын
And even if you filter it, after filtering make up by adding the same solvent. Solvent need not be filtered since your using that as a control in a well. So make up the concentration using the same solvent
@kubra_nik17122 жыл бұрын
Thank you sir, yours vedio is really helpful Could you tell me how to make fungal inoculum culture is it broth or agar medium And to culture Aspergillus niger which broth i have to use
@Dr.Rakesh.B2 жыл бұрын
You can use both to make culture. PDA is used
@kubra_nik17122 жыл бұрын
@@Dr.Rakesh.B thank you
@easychemistryofficial Жыл бұрын
Sir can we use same procedure for Determination of MIC of antifungal agent...
@karishmawaghmare2927 Жыл бұрын
We have prepared methanolic extract so which solvent can be used to check antifungal activity? Kindly provide your suggestion sir.
@Dr.Rakesh.B Жыл бұрын
If you have prepared methanolic extract only methanol can be used. If you need to use different solvents again you need to prepare different extracts. Watch my video about plant extract preparation in my channel to understand.
@sathsaradiwanjan93683 жыл бұрын
Thank you sir, great explanation❤❤❤
@farishtayali18923 жыл бұрын
Ur way of guiding is very impressive and obsorbing
@Dr.Rakesh.B3 жыл бұрын
Means a lot. Thank you :)
@shubhadathorat3522 Жыл бұрын
Sir can you please give some idea about how to check antimicrobial activity of fabric
@savitasandur20372 жыл бұрын
Sir i want to lyophilize my isolates mat, and also for GS-MS, HPLC, FTRI, please let me know the centers in Karnataka.
@shalinis37382 жыл бұрын
Thank you sir..well explained ..sir can you explain comparison between the standard drug , why we should compare ..
@Dr.Rakesh.B2 жыл бұрын
To compare whether your extract is more powerful or commercial drugs
@shalinis37382 жыл бұрын
Ok sir..tq🙏🙏🙏
@reshmidas7019 Жыл бұрын
Great explanation sir Sir i have one question For agar well diffusion, i have to pour the media then put the inoculum n spread it, or i can mix the inoculum with the PDA and then pour it ? Actually i am confused with this thing
@soumyakvijayan6209 Жыл бұрын
Nicely explained Sir, thank you.I have a doubt that,for microbial activity ,do we need to measure the diameter or radius of clear zone?
@Dr.Rakesh.B Жыл бұрын
Radius
@husainagandhi9922 Жыл бұрын
thank you sir this video was really helpfull but i require more knwledge related to this because i m performing my dissertation on turmeric antimicrobial affcts on bacteria which harm the fish so if i can get any help via you would b appreciated
@abdurrahmanmahir4969 Жыл бұрын
Please sir what is the difference between Antibacterial and antitumor activity. My research topic is Antitumor activity of plant extract using Agrobacterium tume Thanks
@Dr.Rakesh.B Жыл бұрын
Antibacterial is how efficiently extracts kill bacteria, antitumor is how efficiently tumor/cancer cells are killed by plant extract
@Artist-z4e2 жыл бұрын
Sir..what is the measurement of microbial inoculum, which added to the plate
@Dr.Rakesh.B2 жыл бұрын
Depends on the well size 10-50 ul
@shubhadathorat3522 Жыл бұрын
Can you give me some idea about taking antibacterial activity of fabric
@neyamurugesan91282 жыл бұрын
Can I use this method with plant oils?
@Dr.Rakesh.B2 жыл бұрын
Yes.
@brijeshkumari6501 Жыл бұрын
Bhaut ache se samjaya thanks sir
@ankitayadav2404 Жыл бұрын
very helpful thankyu so much sir
@hiteshisabharwal49292 жыл бұрын
Helo Sir, Can you tell me how to make the fungal spore solution from the spores?
@Dr.Rakesh.B2 жыл бұрын
Im sorry i haven't done that.
@nielvincansejo2 жыл бұрын
How can positive control be prepared? Should we dissolve it into a certain amount of water? TIA
@Dr.Rakesh.B2 жыл бұрын
Yes
@nielvincansejo2 жыл бұрын
@@Dr.Rakesh.B Can you suggest, Sir, such a published article on the preparation of a positive control? Thank you so much!
@nielvincansejo2 жыл бұрын
May I also ask this one, Sir. Can we use Whatman No.1 filter paper as an alternative for the antibiotic assay disc? If possible, does it affect the ZOI of the assay solution? Thank you so much!
@Dr.Rakesh.B2 жыл бұрын
I don't think so in papers such details for positive control will be given. Anyway u can browse them. If you're using well method directly you can pipette the samples control into well. Disc method requires the disc as it has a known conc. of equally coated drugs which cannot be compensated by filter papers.
@KV.OrganicsАй бұрын
Dear sir, how can I contact you?
@ressurection7278 Жыл бұрын
Sir which solvent should I use for dissolving silver nanoparticles.
@Dr.Rakesh.B Жыл бұрын
Water, check my papers for more information from research gate
@ressurection7278 Жыл бұрын
I did try dmso 10 percent but it doesn't dissolves.
@Dr.Rakesh.B Жыл бұрын
@@ressurection7278 they won't dissolve very easily. Read papers on your work to know how to dissolve
@Skincare1792 жыл бұрын
Please make a video on extract preparation of latex,(dry latex method) since i am finding it very difficult to work on.
@Dr.Rakesh.B2 жыл бұрын
Will check.
@dr.vilaschaudhari2 жыл бұрын
where can i find the disc for disc diffusion method in India?
@Dr.Rakesh.B2 жыл бұрын
Order from merchants who sell scientific products. Google it you will find...or order from Himedia
@tt-ys3zq2 жыл бұрын
Did u use whatman filter paper 1 for making disc with punching machine..pl tell..
@Dr.Rakesh.B2 жыл бұрын
No it's available in market our lab had that.
@tt-ys3zq2 жыл бұрын
@@Dr.Rakesh.B can u pl tell the make
@tt-ys3zq2 жыл бұрын
@@Dr.Rakesh.B and can whatman filter paper be used
@Dr.Rakesh.B2 жыл бұрын
No whatmann doesn't serve the purpose. You can try as a trial and error and check. The one I used was purchased not made. So I don't know the make. You can buy it as sterile disc's from internet. Or you can use even without that by well method or broth method watch that video too.
@tt-ys3zq2 жыл бұрын
@@Dr.Rakesh.B pl tell the manufacturer name
@TasawarFish3 ай бұрын
What are the findings of your research
@TasawarFish3 ай бұрын
Where they applied
@therebel53788 ай бұрын
Sir will you please explain with MIC
@Artist-z4e2 жыл бұрын
Sir.. What is the inoculum density... in your experiment??
@Dr.Rakesh.B2 жыл бұрын
It was for demonstration. All those were not calculated
@TasawarFish3 ай бұрын
Sir what is the importance of this reasearch to Pakistan and whole world
@AlokDas-yj3xb Жыл бұрын
sir please share your paper, the link is expired.
@Dr.Rakesh.B Жыл бұрын
Mail me ill send rakeshb96@yahoo.com
@nurhidayahbazir77162 жыл бұрын
Sir can you explain me about "antimicrobial activity of zno at different ph values" ?? How to prepare this?
@radhikagola22082 жыл бұрын
How can we prepare spore culture of aspergillus?
@Dr.Rakesh.B2 жыл бұрын
Im sorry I haven't performed that.
@VARSHAPBT Жыл бұрын
where I get sterile disc sir..for my pg research work I want sterile disc sir..where I get it sir..help me sir..
@Dr.Rakesh.B Жыл бұрын
Order online from Himedia
@VARSHAPBT Жыл бұрын
thank you sir
@sunitachoudhary36652 жыл бұрын
Sir please explain me . What is the control during antibacterial activity .
@Dr.Rakesh.B2 жыл бұрын
Just the solvent with which you used to prepare the extract. In my Channel 2 videos are there for antimicrobial activities watch both you will understand better
@sunitachoudhary36652 жыл бұрын
@@Dr.Rakesh.B ok . Thanku sir
@TasawarFish3 ай бұрын
What are the main terms of your research
@shrutipandey3127 Жыл бұрын
Very nice
@jankies007 Жыл бұрын
Hi I have two questions related to the topic (+ I just want to say thank you so much, these videos are exceedingly helpful) : 1. if I am testing an antifungal activity of a substance (garbage enzyme from citrus peels to be specific), can I use well diffusion method? 2. what is the most reccomended method for inoculating the fungi? Is it the same method shown in the video (using liquid spore culture first, then the liquid is spread into the plates), or can I simply transfer a portion of a culture inoculated in a plate using a scalpel? Or is there other beter method? I am using Botrytis cinerea as the fungi btw. So it's antifungal activity of garbage enzyme from citrus peel againts Botrytis cinerea From what I can understand so far (by researching the topic on the internet), the standardized/more reccomended method is using broth dillution, but I am also under the impression that well diffusion can also work to test antifungal activity againts Botrytis cinerea from a study I had found titled "A Comparative Study of Antimicrobial and Antioxidant Activities of Plant Essential Oils and Extracts as Candidate Ingredients for Edible Coatings to Control Decay in ‘Wonderful’ Pomegranate" Sidenote : tysm for the content you post here, as a student I find them very useful. It is perfectly understandable if you dont find the time to respond to my very specific questions, and sorry for taking your time
@aimananwar75072 жыл бұрын
For which extract( methanol or ethanol) we use DMSO chemical??
@Dr.Rakesh.B2 жыл бұрын
Dmso is a solvent. Like water , methanol ethanol it is also useful to make extracts.
@aimananwar75072 жыл бұрын
@@Dr.Rakesh.B actually I made ethanol extract and Wan to find its effect on bacteria so ethanol can't be used so I dried it and now I want to dissolve it in dmso so can you tell me how much amout should I use for dried weight of extract
@Dr.Rakesh.B2 жыл бұрын
Concentration you should decide 1 mg/ ml or 10 mg/ml like this based on review of literature
@aimananwar75072 жыл бұрын
@@Dr.Rakesh.B okay thanks😇
@AnjaliSingh-ug7il7 ай бұрын
What do u mean use methanol as control do I use methanol alone on a dic and place it on the media ?? I'm sorry I'm a first sem student I have doubts
@hksm874 ай бұрын
You can use different positive controls such as antibiotics
@sruthyepmthrissur74592 жыл бұрын
Could you please tell the references of this antibacterial study
@sekharsbiofactory84453 жыл бұрын
Great job sir
@lrastina24964 ай бұрын
Can we use sterile filter paper disc ?? I dont gave sterile disc
@hksm874 ай бұрын
Sure. Make the disks and the autoclave them.
@sarram.belili80873 жыл бұрын
Thank you for the video :) I have some questions please: - you are testing an antifungal activity so why are you using an antibiotic as a control? - and, can you please tell me how to prepare plant extract dilutions and what diluent to use?
@Dr.Rakesh.B3 жыл бұрын
Yes you are right, It was just for demonstration to show the audience. I haven't used any standard antimicrobial drugs here. For dilutions its trial and error, refer papers. To make plant extracts u can watch my video in channel
@bety30172 жыл бұрын
@@Dr.Rakesh.B plz sir can you expline how to make. Different concentrations of extrait
@Dr.Rakesh.B2 жыл бұрын
Watch my video on methanolic plant extract preparation in my channel. There I have explained.
@iznaahere2 жыл бұрын
For comparison u used antibiotic disc with what kind of disc? Like i don't get it. Was it not the antibiotic disc in which you added the extract drop wise previously?
@Dr.Rakesh.B2 жыл бұрын
No they are just plain discs which will absorb ur material or samples. You get antibiotic coated disc which we use as std. If you don't get that you can buy a antibiotic from chemist and dissolve it and use that
@iznaahere2 жыл бұрын
@@Dr.Rakesh.B okay so i just knew about the antibiotic discs. Now i came to know that we can find simple discs as well. I got it now. Thanks alot for the guidance. Stay immensely blessed Sir! 💫
@nahushshekar.rnahushshekar25352 жыл бұрын
sir is there any method for anti diabetic activity using microbes
@Dr.Rakesh.B2 жыл бұрын
I have not done anything related to it. I'm sorry I don't know
@Artist-z4e2 жыл бұрын
Triplicate sample testing.......Is this essential for these antimicrobial assay???
@Dr.Rakesh.B2 жыл бұрын
Yes they are to calculate error
@vennilaphysics8740 Жыл бұрын
Super explain
@pavs78273 жыл бұрын
Sir kindly make a vedio on flagella staining sir...
@Dr.Rakesh.B3 жыл бұрын
Ok sure :)
@pavs78273 жыл бұрын
@@Dr.Rakesh.B I'll b waiting for that vedio sir....kindly....
@farishtayali18923 жыл бұрын
Plz share antihemolytic, phytotoxicity and cytotoxicity assay ... Plzzzz
@Dr.Rakesh.B3 жыл бұрын
Ok
@Thisara_Me2 жыл бұрын
Thank you so much ! 👏👏
@madarajayanetti53572 жыл бұрын
How to determine the concentration of the microbial culture?
@Dr.Rakesh.B2 жыл бұрын
Its based on volume thats all. Not quantitative
@fatimaanjum69842 жыл бұрын
Thank you 😊
@unnatirathod80983 жыл бұрын
Very useful
@Dr.Rakesh.B3 жыл бұрын
Thank you :)
@aabsheensaba65323 жыл бұрын
How we make pour culture?
@Dr.Rakesh.B3 жыл бұрын
Using micropipette
@dianahaobam8536 Жыл бұрын
Thank u very much sirrrr🙏
@deepagupta9193 жыл бұрын
Sir how to prepare aloe vera plant extract
@Dr.Rakesh.B3 жыл бұрын
Please do watch the video on plant extract preparation in my channel
@navedanjum7482 жыл бұрын
From where to get antibiotic
@Dr.Rakesh.B2 жыл бұрын
You can take from medical shops/pharmacy. If you want more pure forms or discs you have to order them through companies which sell chemicals
@biryani68612 жыл бұрын
sir how if we have sample venom, which media will be better
@Dr.Rakesh.B2 жыл бұрын
Venom?
@biryani68612 жыл бұрын
@@Dr.Rakesh.B sir actually I'm using the venom of cobra in order to find bioactive peptides that can use for antibacterial activity
@Dr.Rakesh.B2 жыл бұрын
Ok, then venom is usually too toxic and concentrated. You can dilute it with water. Say for eg. 50ul in 450ul water and consider this as your stock. From this pipette different concentrations may be 25, 50, 75, 100 or 50, 100, 150, 200 etc
@biryani68612 жыл бұрын
@@Dr.Rakesh.B Alright sir, thank you very much, sir i have another question at what ic50 value sample will be consider potent against bacteria
@Dr.Rakesh.B2 жыл бұрын
That value varies from sample to sample. But generally lower the value higher the significance
@upscziddi42282 жыл бұрын
Thanku sir for video
@naitikpatel61642 жыл бұрын
How to join you for work sir I want to work with you.
@Dr.Rakesh.B2 жыл бұрын
I finished my PhD I'm teaching for NEET bio now.
@naitikpatel61642 жыл бұрын
@@Dr.Rakesh.B i am doing MSC MICROBIOLOGY i am searching some prectical methods for my research and i found your video and it have so much information about my work. So i wants to join you for work so i gain more knowledge about my fild.
@Dr.Rakesh.B2 жыл бұрын
I don't know of I can be of any help Mail me at rakeshb96@yahoo.com
@chathuminidias9863 Жыл бұрын
Thank you !!!!
@sanidas66273 жыл бұрын
Sir how to prepare plant extract ?
@Dr.Rakesh.B3 жыл бұрын
Follow this method kzbin.info/www/bejne/rGLHeaCIjNWZeJY
@swapnasg59852 жыл бұрын
Sir ,I want to research in antibacterial activity area.....can u please suggest me topic ....actually I done M.Sc in Physics
@Dr.Rakesh.B2 жыл бұрын
You got to read lots of papers to find the gap. It's not so easy for me to suggest you and I'm not that experienced too
@nehabinwal10893 жыл бұрын
Sir is this method is useful for measuring antifungal activity also, i mean in disc diffusion method and well method fungal mycelium is placed in centre of the petridisc or spread all over please reply. I have a confusion Whether to put a fungal mycelium disc in the centre of the agar plate or to spread it
@Dr.Rakesh.B3 жыл бұрын
Yes you can use for fungal. What i did is fungus only
@nehabinwal10893 жыл бұрын
@@Dr.Rakesh.B sir i have to put fungal mycelium disc at centre??