Antimicrobial activity by Broth Dilution Assay with calculations // MIC // IC50 value

[@hemalathasa1547]

Thank you for the video. It is explained very clearly .not possible to know in research papers. Really helpful for my work.

[@Dr.Rakesh.B]

Happy it's helping you 😊

[@Rashdi_]

Thanks God I found your video.

[@howabegam6293]

EXCELLENT.. NICELY EXPLIANED... THANK YOU

[@minib566]

Cen u do for solid media too for dual culture of fungus

[@Md.SadmanHossain-r7x]

It is really a good vedio...

[@amizayasmeen6016]

Excellent. A good way to demonstrate..

[@sarram.belili8087]

Thank you very much Can please do a video about synergy and antagonism of plant extracts

[@garimayadav4306]

Nicely explained..

[@NishmithaRamaraj]

very good explanation

[@dr.b.l.manjula6648]

Well explained..Loved your demonstration. But one should be careful in front of flame while sterilizing pipettes...

[@sivaraj159]

sir it was very fast and spontaneous.but one thing you should concentrate while flaming dont sprinkle alcohol.by using antibiotic you demonstrate.

[@amizayasmeen6016]

Would you like to share a research paper in which this method has been discussed because for research writing we need references.

[@Dr.Rakesh.B]

There are so many papers. Just google using the terms. I have modified accordingly for my research. Once published will let you know.

[@rjknowledgeblogger3618]

Sir, Sir, OD will be taken by normal spectrophotometer or any other instrument. If so, Does chlorophyl in plant extract does not affect OD.

[@anshikagarg1644]

Sir for more sample what should i use??

[@eveberry9215]

Sir what about the control that contained 5ml broth + 20ul inoculum + solvent (e.g. methanol)? You did not mention the formula 9:56

[@divyankumale5303]

Thank you sir for such great video,but i m not able to download your reseach paper that you have provided title and link,kindly share pdf if possible.

[@Dr.Rakesh.B]

Mail me which paper you need [e-mail]

[@JoydebChandraRoy-g8z]

sir.... thank you..for your helpful videos. sir .. I'm a student of Master of science in Entomology .. My research is estimation of protease of entomopathogenic fungus beaveria bassiana by caseine test. sir, g didn't get the protocol of it ... if you make a video on it it will be good for me... I'm looking forward to releasing the video.

[@priyanshugautam9892]

Please tell us how can we calculate on exel Please tell this also

[@s.ch.rajesh]

Nice explanation ❤

[@poulamisarkar3127]

In 5ml of media we are innoculating 20microlitre of innoculum.... But what amout of different antimicrobial agent should be poured in every test tube?

[@mahmoudkassem1801]

Can you provide us with a research paper that use this methode in the same steps you show . Because i saw different research papers that use this method but in diferent way. as an example they do serial dilution and so on . Thank you ❤

[@PujaDas-yo7sh]

What solvent can we use for preparing different concentrations of nanoparticles? Can we use the broth as solvent?

[@Dr.Rakesh.B]

Broth has the media components that will alter the media and favor/limit microbial growth. It is better to use any solvent like water if soluble or organic solvent to dissolve. Incase if ur using any solvents like methanol, chloroform etc then you can take just this solvent as one of your control too

[@PujaDas-yo7sh]

@@Dr.Rakesh.B thank you sir

[@sudeepa5851]

the link for paper is not opening. Resend plz

[@reshmabenson1812]

Sir which bacteria you choose for the test

[@jigyasasahu7717]

Is it compulsory to get straight line in graph in MIC..???

[@unzishqamar4934]

Thank you for this video kindly suggest some research papers related to that video

[@aartiuprikar5343]

explain how the concentration is taken for MIC of extract which dissolved in DMSO

[@atrayeedutta6531]

Can I use the same protocol to find out the antifungal activity?

[@Adviknaidu5458]

Sir what about cfu in inoculum specifically in case of fungi to calculate

[@aartiuprikar5343]

I used different extract of leaves then solidify and dissolved in DMSO and make different concentration for agar well diffusion method but for MIC solidify extract is dissolved in DMSO or water please clear the confusion and also DMSO and ethanol used as a control or not if ethanolic extract is used

[@Dr.Rakesh.B]

Water and dmso both you can use. If ur using dmso take dmso as control. If it's ethanol extract, when redissolving use ethanol only and take the same as control

[@aartiuprikar5343]

thank you sir

[@aartiuprikar5343]

I use ethanol, acetone, ethyl acetate, petroleum ether extract and then solidify and dissolved in DMSO then I use DMSO as a control or ethanol or other solvent as a control

[@shellysharma1693]

Hii Sir, thanks you for this video. I had a question that if my solvent is in a gel form and my plant extract is in powder then how much amount of powder will i mix in what concentration of gel for disc diffusion method ?

[@jeniferjames2448]

Hello sir I have a doubt I have a stock solution of 100mg/ml. If I take 100ul from this solution and add it to the well containing 100ul broth and serial dilute it what will be the concentration (mg/ml) in the first well. Please reply soon sir.

[@bety3017]

How to make different concetration od plant extra, plz sir help me

[@sruthyepmthrissur7459]

Sir could you please tell me what you take as blank for reading

[@Dr.Rakesh.B]

Just the Media

[@sreevidyasankarasubramania733]

Thank you once again for a very clear explanation. How do you find IC 50 by using excel, can you help me in finding out that, from % inhibition using DPPH assay.

[@kirtyferwani7695]

Sir will the value for test and non cross test differ by microbroth technique

[@Dr.Rakesh.B]

What is non cross test?

[@pavs7827]

Sir can we do this for bactericidal chemicals??

[@Dr.Rakesh.B]

Yes you can

[@pavs7827]

@@Dr.Rakesh.B ok sir thank you

[@MsRavitheja]

Sir to test MIC for marine organisms should the broth be supplimented with salt

[@Dr.Rakesh.B]

Marine microorganisms?? In our normal media itself we have NaCl. So it should be fine . You can modify the normal media based on their growth. Refer research papers for more information

[@ankitnegi8070]

Sir if I have a methanolic extract of plant what should be the control. Media plus methanol or media only

[@Dr.Rakesh.B]

You will just add methanol to the media and the bacterial inoculum

[@ankitnegi8070]

Sir kya mujh apka email address ya koi contact number mil sakta hai.... I have so many queries

[@bhushansong]

Thank you. Very nice explanation

[@basantasarkar7475]

What is the plant extract volume used against 5 ml broth here ?

[@Dr.Rakesh.B]

It is the concentration that matters. Here i have taken 100 to 1000 ul.

[@atrayeedutta6531]

Sir Please share the reference of the protocol.

[@Dr.Rakesh.B]

For this reference I don't have. You can search any research papers

[@atrayeedutta6531]

Yeah I already searched a lot of papers. But I am unable to find the exact reference for this method. Please share if it is possible for you. Thank you

[@Dr.Rakesh.B]

I don't have. My paper is under publication. You can follow me in research gate or Google Scholar for updates.

[@atrayeedutta6531]

Thank you sir

[@kishoredinesh2441]

Bro I need a article containing this procedure

[@Dr.Rakesh.B]

Mail me [e-mail]

[@kishoredinesh2441]

@@Dr.Rakesh.B okay sir

[@jialewee3922]

Hi Sir. Thank you for the video. I have several questions after watching the video. 1. You didn't mention about the bacteria concentration during the assay. Is it necessary to have a specific concentration? Or as long as the concentration applied is the same then it's fine? 2. What is the blank you used when measuring the OD? Is it the broth + sample (that having the same concentration as tested sample)? 3. Isn't the MIC should be at least 80% of growth inhibition? Hope to hear from you soon. Thanks

[@Dr.Rakesh.B]

Yes the conc. Will be in different volume. You might take 100ul, 200 ul or 0.5ml, 1.0ml like this. You cannot count bacteria to know its concentration so volume based concentration you might take. Blank is just the media what you use. There's no fixed rule for MIC it varies from bacteria to bacteria plant to plants.

[@krunaldholiya8502]

thnak you for explaining it nicely, but what you shown here is "50% of assay dynamic range"

[@artivarma6066]

Hello sir, want to check the antimicrobial activity of hing essential oil by using cup borer method so for that which solvent I can use to dilute the essential oil and how to dilute?

[@Dr.Rakesh.B]

Oils are not soluble in water generally so use a appropriate solvent which is soluble in oil. Check papers for the solvent and concentrations

[@seemakedar3865]

👸👸👸

[@aishwaryaudhayakumar7339]

Sir can you please give your mail d tomorrow I have viva I have doubt in calculation

[@rosujha]

Kindly understand ur audience, layman wont be one.