How to prepare plant extracts using various solvents||Methanolic extract

[@zs7785]

I am following your videos in my PhD work

[@RockBison-qb3of]

Bro can you tell me the reference he's is using

[@uzmasubhan877]

Wonderful session... 😊

[@ABCDEFGHIJKLMNOPQ209]

That was so informative thank you so much. I was struggling with this to understand through paper for so long and your video cleared everything

[@priyadarshinichanyal1045]

Thank you SIR ! Much needed video.

[@zs7785]

Your video is extremely informative Sir. Thank you so much 🙏

[@Dr.Rakesh.B]

:)

[@zs7785]

Please keep posting more videos regarding phytochemical analysis of the plant and also about essential oils of the plants.

[@ArslanAli-jn4hd]

Thanks Brother!! Truly helpful.

[@bepratibha382]

very clear explaination...👌👌🙏👍👍

[@muntasirkamal1112]

Thank you for the clear explanation. I have a question- after methanol extraction and evaporating the methanol, when you re-dissolve the plant residue, can you use any solvent other than methanol? Can I re-dissolve the residue in DMSO to make 1 mg/mL extract in DMSO? Will it dissolve in DMSO? For my experiment I cannot use methanol. It must be DMSO that's why I am asking. Thanks.

[@sagarikaparida4930]

Very much clear and informative

[@jayadevikarthikeyan4678]

Thank you sir I had one doubt can we do methanolic extract of plant from fresh plant material or not?

[@Dr.Rakesh.B]

No not advised. As moisture is there its not accurate.

[@sakshithakur8927]

Hi sir Just need to know one thing, I have done serial plant extraction using pet ether, chcl3, and ethanol.. so will u plz tell me how can I make 1mg/ml solution of chcl3 and pet ether extract for TFC TAc, etc. Like which solvent I can use to dissolve these solvent extract for my further analysis

[@nusratbibi3248]

Sir very good job done by you kindly share videos related to bacterial research related to metal NPs and ethyl acetate extraction of bacterial metabolites

[@satyamt.3059]

Thank you so much. Can you share plant extract preparation in different solvent for GCMS analysis, so that I can send the sample for analysis to other lab. Thanks again.

[@rohitpaithane4065]

Hello Sir. I have some queries about plant collection and drying processes. 1. Can we pluck leaves and leave the plucked leaves to dried separately? 2. Can we cut the roots and leaves separately and dried and powdered at the same time. 3. How long time we should dry the plant sample in shaded area? 4. After powder prepared, how long time we can use powder to carry various experiments? I request you to please answer my doubts.

[@ankitachowdhury5268]

Thnkyou so much sir for your effort....your videos are very beneficial ......

[@carol_ronydalmet9]

How do you store powder? I was planning to put it in a ziplock bag. Shall I store it in refrigerator or room temperature?

[@Dr.Rakesh.B]

Ziplock will do or any air tight vials and refrigerator (freezer).

[@binitayadav8165]

Sir i m doing phytoremediation of BPA.. Nd for extraction after drying i grind the sample with buffer nd aftr that i put methanol. Will it hamper in any way?

[@dr.b.l.manjula6648]

Well explained Rakesh...

[@Dr.Rakesh.B]

Thank you mam 🙏

[@varshinipreethi3189]

Sir wonderful explanation i have a doubt which analysis is best to test the phytochemicals in plant extract? Is GCMS is good?

[@akshayapramod4189]

Sir I have a doubt . I was asked to take the quantity of the sample to be dissolved in the solvent according to the concentration of the sample. Can you tell me how to check whether the sample is concentrated ? Is it by keeping the sample in the solvent i.e; 1:10 ratio overnight and if the solvent evaporates to 3/4 th it means my sample is concentrated Sir?

[@easychemistryofficial]

Very nice👌

[@wistonphiri4995]

is there a specific number of days that medicinal plant extracts are supposed to stay before bioassay screening?

[@Dr.Rakesh.B]

No you can test them immediately. Maximum of 1 month u can store at 0c

[@wistonphiri4995]

@@Dr.Rakesh.B thanks very much Dr. I am enjoying your videos. How about adding 1mL of 70% ethanol as a preservative and store them At 4°C ?

[@Dr.Rakesh.B]

It will change the solubility of chemical compounds

[@numerasaghir2515]

@Dr.Rakesh.B SIR I need to know. If I have to synthesize nanoparticles out of ethanolic extract of powdered seeds of some plant. Do I have to completely remove ethanol from our extract or can I use it in concentrated form coz I can't evaporate it completely.?

[@Dr.Rakesh.B]

You can make a liquid extract and use that liquid.

[@numerasaghir2515]

@@Dr.Rakesh.B liquid extract in ethanol?.

[@Dr.Rakesh.B]

@numerasaghir2515 yes

[@numerasaghir2515]

@@Dr.Rakesh.B this will not impose some bad effect on my nanoparticles na?.

[@aminaqureshi4194]

Please make a video on work mechanisms of rotary evaporator? And is there any simple method to remove solvent?

[@deannaaudrey3014]

Thank you for the video! My project is to identify the best brewing temperature and time for green tea leaves to exhibit the most antioxidant capacity. Can I directly use the sample ( solution of tea and water) to carry out DPPH essay?

[@madansharma2700]

Can one do vacuum (room temperature or lower) drying.

[@bintenaeem8315]

sir , My topic of research is to determine the invitro antidiabetic properties of the medicinal plants. A humble request to you to make a video on this assay.I always watch your videos before performing assays.plz make the video as soon as possible .

[@Dr.Rakesh.B]

Im not working in lab. Im afraid that i can't be doing any experiments for a while.

[@bintenaeem8315]

sir as you perform dpph and other assay so i told you for this bcz these videos helps me alot

[@mehwishhameed9054]

If we do centrigation, then how we can make different concentration of extract for antibacterial activity?

[@dhananjaysharma1371]

It is so useful sir Thanks 👍

[@narmathag208]

Hi sir, lets say i have prepared my methanolic extract for 1mg/ml, i want to further dilute it for some range of concentrations, can i use distilled water to dilute ? this is especially for total phenolic content.

[@MHui-nj4dq]

Thank you for the video, sir. I was wondering what concentration of extract (in mg/mL) is needed for the DPPH assay? Thanks!

[@lakykhatun7119]

Yes sir i have the same question

[@surjitdeka7092]

If the plant extract is sticky and oily then is it possible to make powder?? I have got the sticky extract which is oily..after removal of methanol Can I use it for quantitative analysis

[@Dr.Rakesh.B]

Yes u can

[@surjitdeka7092]

@@Dr.Rakesh.B Thank you so much sir

[@katyperry5570]

Sir how many days is the macerated drug kept in beaker in methanol solvent to drying process

[@Dr.Rakesh.B]

3-4 days

[@aminaqureshi4194]

I need the cellulose contents in gel or viscous form , so is there any method to get gel sample?

[@fouziyayousuf6436]

Thanks a lot sir 🤝❤️

[@jana-t6y7f]

hello, what do we need to preserve the extracts if we will use only for a few days? is anything necessary to add or no need?

[@almasmulla3791]

Very informative video sir. Thank you for uploading. I have a plant whose phytoconstituents are not known. M planning for tlc bioautography can you help me how to select solvent system?

[@madansharma2700]

Good effort, but falls short.

[@abhijeetdarade7819]

Killler Absolutely brilliant

[@Dr.Rakesh.B]

Thank you 🙏

[@priyanshukul1]

Are these extracts can be consumed by normal person?

[@navitagujja9903]

Can we use ethanol imstead of methanol for extracting antioxidants from plants? because i want to use the extract in food. So could you help please.

[@DAVLBOSS124]

Yes u can.

[@tejashreesande1418]

very very useful video...sir will you please tell..for extraction how many solvents we can take ideally?

[@Dr.Rakesh.B]

It depends on the work, is it Ph.D or Msc project. Ideally we take 2 polar solvents and 2 non polar solvents. You can increase if you have time and interest.

[@tamanna8754]

Sir can i use the fresh plant material for methanolic extract . For eg can i homogenise the sample directly in methanol, centrifuge amd use the supernatant for DPPH assays

[@Dr.Rakesh.B]

Yes

[@easychemistryofficial]

It was very informative... sir I have a question... after evaporation I have the extract in pasty form can I dilute it in the same way as u have shared in ur video?

[@kalpanarautela320]

Thank you for easy explanation of procedures. Sir, could you please provide a reference for this procedure... ? Thank you

[@Dr.Rakesh.B]

Usually no paper provides or explain this as it's a well known basic procedure. Not discovered by any particular person to give reference. You can watch my video on references and download my paper and cite the same

[@nehagaikwad3563]

Sir I have performed extraction of plant in microwave by using methanol solvent and I have to prepare methanolic extract to detect flavonoids on tlc plate please suggest me how to do that

[@aimananwar7507]

Can you please tell me how much quantity of orange peel powder and ethanol should I use to make extract to find out inhibition zone of bacterial strains. Please tell.

[@Dr.Rakesh.B]

It's all trial error I can't say just like that

[@aimananwar7507]

@@Dr.Rakesh.B okay

[@m123vihan6]

Sir j plant extract bny h vo 20microlitre m hi hoga ky ml m nhi hoga ky

[@unzilamalik6823]

Thankyou, it helped me quite alot.😃

[@Dr.Rakesh.B]

Happy its helping:)

[@foodotechos7600]

Nice video sir, 1f 10 mg is required for 10 ml methanol, how much methanol will be required for 0.32 mg

[@wistonphiri4995]

320mL

[@zahrakhan805]

Thankyou . Its quite helpful.

[@purabidas1491]

Sir I have one doubt, can we dissolve the powdered methanolic extract in oil

[@Dr.Rakesh.B]

Yes but not completely. Depends on the polarity of the compounds present in extract.

[@purabidas1491]

Sir, can we make a nanoemulgel with w/o nanoemulsion.

[@deepakkhatak9271]

Hello sir I extracted my phytochemicals using n-hexane and I dried it, now can I dissolve this dried extract into some other solvents like methanol or DMSO for my further experiment

[@Dr.Rakesh.B]

No You should use n-hexane only

[@deepakkhatak9271]

@@Dr.Rakesh.B Thank you

[@easychemistryofficial]

Sir in hot air oven up to how much time I should keep the extract for further evaporation after rotavap n also tell plz that is dryer oven n hot air is same...

[@Dr.Rakesh.B]

As long as you can regulate the temperature any oven is fine. Keep for 2-3 days at 40 degrees

[@aswanivmanoj8115]

Thank you so much sir

[@bollywoodboowal8067]

Sir plz make more videos on phd experiment

[@arudd643]

Is this same for water extraction also?

[@syedibrahim9527]

good morning sir i want to know about plant extract for antimicrobial activities .....because this is my research topic ..plz guide me ...

[@somnathdhar1217]

Sir you are great.

[@Dr.Rakesh.B]

Means a lot. Thank you :)

[@ritusolanki]

I dried my Methanolic, acetone and aqueous solvent at 45° in oven for 3?? 4 days. Is my sample can't be used for phenol estimation

[@siddhip3588]

Sir...I have a question..suppose we have no idea regarding solubility characteristic of our sample....then we have to follow successive solvent extraction method with polar to nopolar solvent or vice versa..Right? After extracting with all solvent...What will v do..we will calculate % yield for all extract or what? Before proceeding or selecting extract for any activity....pls rply soon I have to do an activity but no idea abt solubility ...which solvent should i use for preparing extract?

[@Dr.Rakesh.B]

You have to read literature for ur paper and discuss with your supervisor

[@siddhip3588]

@@Dr.Rakesh.B thnx for replying sir......but no sufficient review is available for the same....

[@sangeethak8110]

I am Extracting oil from raw leafy vegetables without drying and powder by using soxhlet apparatus. How to prepare sample for total phenolic content?

[@Dr.Rakesh.B]

Consider the oil directly to be ur sample and proceed. But i don't know if this will work i haven't worked on oils. Try reading papers on oil or u can ask some experienced person in research gate.

[@awaam395]

Sir how can we calculate the amount of herb needed for a certain amount of liquid extract? E.g. if we need 0.5 litre of extract for storage then how much herb will be needed for extraction?

[@Dr.Rakesh.B]

For 1 ml of extract usually 20-25gms of dry weight is required. Its all trial and error cannot be predicted.

[@archanashukla7632]

Very informative video

[@sonicarathore9505]

Sir plz reply how to use fresh sample for flavonoid content and phenolic content determination

[@benjaminchanges7336]

Sir, Can I use this for plant protein extraction before using ultrasonication?

[@nazishjameel-o2v]

Sir I have evaporated solvent by using rotary apparatus. But now they become dry and get struck with plate. so how can I use them??

[@Dr.Rakesh.B]

Take 1 gm dissolve in 10 ml and prepare a stock

[@NazishJameel-oy5yt]

Sir for how long time stock will use؟​@@Dr.Rakesh.B

[@shikharani4973]

It is necessary to further dissole it in a methano? Cant we leave the compound undissolved in -20

[@Dr.Rakesh.B]

You have to dissolve it in known volume of methanol so that you know the concentration. If ur experiments require crude extract in solid form then u can leave as it is and store it

[@shikharani4973]

@@Dr.Rakesh.B thanku sir for replying

[@sarangabro3847]

Hello Sir, I am doing an experiment on a ethanol plant extract that I bought from a well known company. I want to check its antibacterial actions against some bacterial strains. I found no literature about the preparation of the solution of the commercially available ethanol extract of the plant. Therefore, could you please guide me as how to make the solution to check the antibacterial effects of the extract? Thanks

[@vegetablescience8373]

Can we crush sample in liquid nitrogen to make powder?

[@Dr.Rakesh.B]

Such Lower temperature might affect the effectiveness

[@lakykhatun7119]

Sir is it okey if i use the liquid i mean methanol in which the grind sample pourend first?

[@Dr.Rakesh.B]

Yes

[@Xyz_curious]

1. Is the procedure sane for plain water extracts of plants? 2. Can these falcon tubes be used for Long term storage of extract?

[@Dr.Rakesh.B]

Yes for both

[@Xyz_curious]

@@Dr.Rakesh.B thank you sir

[@Xyz_curious]

@@Dr.Rakesh.B sir wat about evaporating step? It will also be sam fr water extracts?

[@Dr.Rakesh.B]

Yes since it's water it will take more time if you keep in room temperature. You can keep it at 40-50 degrees.

[@Xyz_curious]

@@Dr.Rakesh.B what will happen if I skip the evaporation step?

[@preetipatil631]

Hello sir,may i know how to estimate the concentration of plant extract using UV double beam spectrophotometer?

[@priyankakesavan546]

Thank you for the video sir. In my case I have to extract the chlorophyll-a from algae, so do I have to store the filter paper with solvent for 24 hours in the refrigerator before grinding it or should I have to grind and then store it in the refrigerator

[@Dr.Rakesh.B]

Chlorophyll estimation is done by arnons method using 80% chilled acetone..Browse with this keywords you will find papers or in one video in my channel theres a video about references. Watch that video and download my paper for reference

[@rajakumaris8909]

Super sir

[@Akash_DC]

Can you please tell me the research papers which you followed make these videos like Preparation of plant extract, DPPH, TPC

[@Dr.Rakesh.B]

There's a separate video in my channel for references. Please Watch that

[@Mppscwithchauhan]

Can i use normal filter paper ?

[@Dr.Rakesh.B]

Yes

[@ruksanabegamchowdhury5158]

sir i hv extracted volatile oil from hydrodistillation method now i want to dilute the oil in hexane for GCMS analysis...how can i dilute the sample can u plz guide me...i hv vry less amount of sample n i hv to perform further exleriment with that oil

[@Dr.Rakesh.B]

For gcms 1:100 dilution also will work. It's highly sensitive. So dilute according to your other experiments. Just 10ul will be injected to gcms that's all

[@ruksanabegamchowdhury5158]

tnq sir

[@titelmegu9632]

Sir, is it necessary for also vit c quantification ?

[@easychemistryofficial]

Sir I rinsed my dried extract from rotavap flask with corresponded solvent and keep it in open air to dry the sample completely but it is taking long time what should I do? Ur response will b appreciated...

[@Dr.Rakesh.B]

Use oven keep it at 40-45 degrees

[@easychemistryofficial]

@@Dr.Rakesh.B Any type of oven means can I use microwave oven or not? Thanks 4 ur response😊

[@Dr.Rakesh.B]

No you have to leave it for 2-3 days that's not possible in microwave oven. I had meant hot air oven

[@easychemistryofficial]

@@Dr.Rakesh.B thanks🥰

[@muskansheikh262]

How much percentage of ethanol is used in this process

[@nikshithajain25]

Thank you brother ☺️

[@upscziddi4228]

Does Brahmi plant get extract by using hot water or cold water?

[@Dr.Rakesh.B]

Refer from any published paper. It's not possible to know everything for everyone.

[@nitinnangnurkar2840]

Bro pls explain how to make mother tincture ?

[@nikkitaverma7354]

Sir for our project we need to prepare the methanolic extract of aloe vera gel. Can you please guide us on how to proceed for it.

[@bhartirohilla3017]

How long we use crude extract for further activity

[@easychemistryofficial]

Sir same extract is used for antimicrobial activity?

[@Dr.Rakesh.B]

Yes you can use

[@shilpasharma4383]

Gr8 video sir

[@sanjaykp1098]

Is the method can be used for other sample? Means other than plant samples

[@shreyakaushal8296]

Sir how can I mail you? I want to ask about one plant extract preparation for my master’s thesis sir

[@gauravmishra2121]

How to take extraction from euphorbia plant

[@ypdkm3340]

Hello sir, can i use freeze drying to evaporate the methanol after filtration?

[@anjanajayachandran6819]

Sir for the extraction of saussurea lappa , is methanol or ethanol is good?

[@Dr.Rakesh.B]

Please read literatures related to it.

[@mohdyaseen4531]

sir could you please mention the article for references purpose. thank you

[@Dr.Rakesh.B]

I have made a separate video for references pls watch it

[@priyamohan5255]

good evening sir sir can we perform antibacterial activity of plant powder without any extraction if it is possible kindly guide me i m working on a herbo mineral preparation for my dissertation work

[@Dr.Rakesh.B]

No, you have to dissolve it in some solvent .

[@masoodahmedunar6971]

How to do water exract of Chlorophytum Borivilianum Sufed Musli for human research use? plz answer soon I have to present my topic in interview

[@Dr.Rakesh.B]

It will be done same way as this. You can watch other videos related to fungal extract preparation for more info

[@anusihag16]

If solvent is water.. how to obtain powder form of plant extract..lyophilizer or just evaporation in oven??

[@Dr.Rakesh.B]

Oven will do the work. Just that a day or two extra time will be required.

[@anusihag16]

@@Dr.Rakesh.B thanks💫

[@aartiAtkar]

Can we use water as a solvent

[@Dr.Rakesh.B]

Yes

[@dianahaobam8536]

Sir our guide let us make in 70 percent methanol....did u used absolute methanol means that 99.9 percent one?

[@Dr.Rakesh.B]

Both is fine

[@dianahaobam8536]

@@Dr.Rakesh.B thank u