That was so informative thank you so much. I was struggling with this to understand through paper for so long and your video cleared everything
@priyadarshinichanyal10457 ай бұрын
Thank you SIR ! Much needed video.
@zs77852 жыл бұрын
Your video is extremely informative Sir. Thank you so much 🙏
@Dr.Rakesh.B2 жыл бұрын
:)
@zs77852 жыл бұрын
Please keep posting more videos regarding phytochemical analysis of the plant and also about essential oils of the plants.
@ArslanAli-jn4hd2 ай бұрын
Thanks Brother!! Truly helpful.
@bepratibha3827 ай бұрын
very clear explaination...👌👌🙏👍👍
@muntasirkamal1112 Жыл бұрын
Thank you for the clear explanation. I have a question- after methanol extraction and evaporating the methanol, when you re-dissolve the plant residue, can you use any solvent other than methanol? Can I re-dissolve the residue in DMSO to make 1 mg/mL extract in DMSO? Will it dissolve in DMSO? For my experiment I cannot use methanol. It must be DMSO that's why I am asking. Thanks.
@sagarikaparida49303 жыл бұрын
Very much clear and informative
@jayadevikarthikeyan46786 күн бұрын
Thank you sir I had one doubt can we do methanolic extract of plant from fresh plant material or not?
@Dr.Rakesh.B6 күн бұрын
No not advised. As moisture is there its not accurate.
@sakshithakur89272 жыл бұрын
Hi sir Just need to know one thing, I have done serial plant extraction using pet ether, chcl3, and ethanol.. so will u plz tell me how can I make 1mg/ml solution of chcl3 and pet ether extract for TFC TAc, etc. Like which solvent I can use to dissolve these solvent extract for my further analysis
@nusratbibi324811 ай бұрын
Sir very good job done by you kindly share videos related to bacterial research related to metal NPs and ethyl acetate extraction of bacterial metabolites
@satyamt.305910 ай бұрын
Thank you so much. Can you share plant extract preparation in different solvent for GCMS analysis, so that I can send the sample for analysis to other lab. Thanks again.
@rohitpaithane4065Ай бұрын
Hello Sir. I have some queries about plant collection and drying processes. 1. Can we pluck leaves and leave the plucked leaves to dried separately? 2. Can we cut the roots and leaves separately and dried and powdered at the same time. 3. How long time we should dry the plant sample in shaded area? 4. After powder prepared, how long time we can use powder to carry various experiments? I request you to please answer my doubts.
@ankitachowdhury52684 жыл бұрын
Thnkyou so much sir for your effort....your videos are very beneficial ......
@carol_ronydalmet9 Жыл бұрын
How do you store powder? I was planning to put it in a ziplock bag. Shall I store it in refrigerator or room temperature?
@Dr.Rakesh.B Жыл бұрын
Ziplock will do or any air tight vials and refrigerator (freezer).
@binitayadav81654 ай бұрын
Sir i m doing phytoremediation of BPA.. Nd for extraction after drying i grind the sample with buffer nd aftr that i put methanol. Will it hamper in any way?
@dr.b.l.manjula66483 ай бұрын
Well explained Rakesh...
@Dr.Rakesh.B3 ай бұрын
Thank you mam 🙏
@varshinipreethi31893 жыл бұрын
Sir wonderful explanation i have a doubt which analysis is best to test the phytochemicals in plant extract? Is GCMS is good?
@akshayapramod4189 Жыл бұрын
Sir I have a doubt . I was asked to take the quantity of the sample to be dissolved in the solvent according to the concentration of the sample. Can you tell me how to check whether the sample is concentrated ? Is it by keeping the sample in the solvent i.e; 1:10 ratio overnight and if the solvent evaporates to 3/4 th it means my sample is concentrated Sir?
@easychemistryofficial2 жыл бұрын
Very nice👌
@wistonphiri49958 ай бұрын
is there a specific number of days that medicinal plant extracts are supposed to stay before bioassay screening?
@Dr.Rakesh.B8 ай бұрын
No you can test them immediately. Maximum of 1 month u can store at 0c
@wistonphiri49958 ай бұрын
@@Dr.Rakesh.B thanks very much Dr. I am enjoying your videos. How about adding 1mL of 70% ethanol as a preservative and store them At 4°C ?
@Dr.Rakesh.B8 ай бұрын
It will change the solubility of chemical compounds
@numerasaghir2515 Жыл бұрын
@Dr.Rakesh.B SIR I need to know. If I have to synthesize nanoparticles out of ethanolic extract of powdered seeds of some plant. Do I have to completely remove ethanol from our extract or can I use it in concentrated form coz I can't evaporate it completely.?
@Dr.Rakesh.B Жыл бұрын
You can make a liquid extract and use that liquid.
@numerasaghir2515 Жыл бұрын
@@Dr.Rakesh.B liquid extract in ethanol?.
@Dr.Rakesh.B Жыл бұрын
@numerasaghir2515 yes
@numerasaghir2515 Жыл бұрын
@@Dr.Rakesh.B this will not impose some bad effect on my nanoparticles na?.
@aminaqureshi41945 ай бұрын
Please make a video on work mechanisms of rotary evaporator? And is there any simple method to remove solvent?
@deannaaudrey30143 жыл бұрын
Thank you for the video! My project is to identify the best brewing temperature and time for green tea leaves to exhibit the most antioxidant capacity. Can I directly use the sample ( solution of tea and water) to carry out DPPH essay?
@madansharma27003 ай бұрын
Can one do vacuum (room temperature or lower) drying.
@bintenaeem83152 жыл бұрын
sir , My topic of research is to determine the invitro antidiabetic properties of the medicinal plants. A humble request to you to make a video on this assay.I always watch your videos before performing assays.plz make the video as soon as possible .
@Dr.Rakesh.B2 жыл бұрын
Im not working in lab. Im afraid that i can't be doing any experiments for a while.
@bintenaeem83152 жыл бұрын
sir as you perform dpph and other assay so i told you for this bcz these videos helps me alot
@mehwishhameed90542 ай бұрын
If we do centrigation, then how we can make different concentration of extract for antibacterial activity?
@dhananjaysharma13712 жыл бұрын
It is so useful sir Thanks 👍
@narmathag2082 жыл бұрын
Hi sir, lets say i have prepared my methanolic extract for 1mg/ml, i want to further dilute it for some range of concentrations, can i use distilled water to dilute ? this is especially for total phenolic content.
@MHui-nj4dq3 жыл бұрын
Thank you for the video, sir. I was wondering what concentration of extract (in mg/mL) is needed for the DPPH assay? Thanks!
@lakykhatun71192 жыл бұрын
Yes sir i have the same question
@surjitdeka70922 жыл бұрын
If the plant extract is sticky and oily then is it possible to make powder?? I have got the sticky extract which is oily..after removal of methanol Can I use it for quantitative analysis
@Dr.Rakesh.B2 жыл бұрын
Yes u can
@surjitdeka70922 жыл бұрын
@@Dr.Rakesh.B Thank you so much sir
@katyperry5570 Жыл бұрын
Sir how many days is the macerated drug kept in beaker in methanol solvent to drying process
@Dr.Rakesh.B Жыл бұрын
3-4 days
@aminaqureshi41945 ай бұрын
I need the cellulose contents in gel or viscous form , so is there any method to get gel sample?
@fouziyayousuf6436 Жыл бұрын
Thanks a lot sir 🤝❤️
@jana-t6y7f9 ай бұрын
hello, what do we need to preserve the extracts if we will use only for a few days? is anything necessary to add or no need?
@almasmulla37913 жыл бұрын
Very informative video sir. Thank you for uploading. I have a plant whose phytoconstituents are not known. M planning for tlc bioautography can you help me how to select solvent system?
@madansharma27003 ай бұрын
Good effort, but falls short.
@abhijeetdarade78192 жыл бұрын
Killler Absolutely brilliant
@Dr.Rakesh.B2 жыл бұрын
Thank you 🙏
@priyanshukul16 ай бұрын
Are these extracts can be consumed by normal person?
@navitagujja990312 күн бұрын
Can we use ethanol imstead of methanol for extracting antioxidants from plants? because i want to use the extract in food. So could you help please.
@DAVLBOSS12412 күн бұрын
Yes u can.
@tejashreesande14183 жыл бұрын
very very useful video...sir will you please tell..for extraction how many solvents we can take ideally?
@Dr.Rakesh.B3 жыл бұрын
It depends on the work, is it Ph.D or Msc project. Ideally we take 2 polar solvents and 2 non polar solvents. You can increase if you have time and interest.
@tamanna8754 Жыл бұрын
Sir can i use the fresh plant material for methanolic extract . For eg can i homogenise the sample directly in methanol, centrifuge amd use the supernatant for DPPH assays
@Dr.Rakesh.B Жыл бұрын
Yes
@easychemistryofficial2 жыл бұрын
It was very informative... sir I have a question... after evaporation I have the extract in pasty form can I dilute it in the same way as u have shared in ur video?
@kalpanarautela3202 жыл бұрын
Thank you for easy explanation of procedures. Sir, could you please provide a reference for this procedure... ? Thank you
@Dr.Rakesh.B2 жыл бұрын
Usually no paper provides or explain this as it's a well known basic procedure. Not discovered by any particular person to give reference. You can watch my video on references and download my paper and cite the same
@nehagaikwad3563 Жыл бұрын
Sir I have performed extraction of plant in microwave by using methanol solvent and I have to prepare methanolic extract to detect flavonoids on tlc plate please suggest me how to do that
@aimananwar75072 жыл бұрын
Can you please tell me how much quantity of orange peel powder and ethanol should I use to make extract to find out inhibition zone of bacterial strains. Please tell.
@Dr.Rakesh.B2 жыл бұрын
It's all trial error I can't say just like that
@aimananwar75072 жыл бұрын
@@Dr.Rakesh.B okay
@m123vihan65 ай бұрын
Sir j plant extract bny h vo 20microlitre m hi hoga ky ml m nhi hoga ky
@unzilamalik68233 жыл бұрын
Thankyou, it helped me quite alot.😃
@Dr.Rakesh.B3 жыл бұрын
Happy its helping:)
@foodotechos760011 ай бұрын
Nice video sir, 1f 10 mg is required for 10 ml methanol, how much methanol will be required for 0.32 mg
@wistonphiri49958 ай бұрын
320mL
@zahrakhan8053 жыл бұрын
Thankyou . Its quite helpful.
@purabidas14918 ай бұрын
Sir I have one doubt, can we dissolve the powdered methanolic extract in oil
@Dr.Rakesh.B8 ай бұрын
Yes but not completely. Depends on the polarity of the compounds present in extract.
@purabidas14918 ай бұрын
Sir, can we make a nanoemulgel with w/o nanoemulsion.
@deepakkhatak9271 Жыл бұрын
Hello sir I extracted my phytochemicals using n-hexane and I dried it, now can I dissolve this dried extract into some other solvents like methanol or DMSO for my further experiment
@Dr.Rakesh.B Жыл бұрын
No You should use n-hexane only
@deepakkhatak9271 Жыл бұрын
@@Dr.Rakesh.B Thank you
@easychemistryofficial2 жыл бұрын
Sir in hot air oven up to how much time I should keep the extract for further evaporation after rotavap n also tell plz that is dryer oven n hot air is same...
@Dr.Rakesh.B2 жыл бұрын
As long as you can regulate the temperature any oven is fine. Keep for 2-3 days at 40 degrees
@aswanivmanoj8115 Жыл бұрын
Thank you so much sir
@bollywoodboowal80677 ай бұрын
Sir plz make more videos on phd experiment
@arudd643 Жыл бұрын
Is this same for water extraction also?
@syedibrahim9527 Жыл бұрын
good morning sir i want to know about plant extract for antimicrobial activities .....because this is my research topic ..plz guide me ...
@somnathdhar12172 жыл бұрын
Sir you are great.
@Dr.Rakesh.B2 жыл бұрын
Means a lot. Thank you :)
@ritusolanki3 жыл бұрын
I dried my Methanolic, acetone and aqueous solvent at 45° in oven for 3?? 4 days. Is my sample can't be used for phenol estimation
@siddhip35882 жыл бұрын
Sir...I have a question..suppose we have no idea regarding solubility characteristic of our sample....then we have to follow successive solvent extraction method with polar to nopolar solvent or vice versa..Right? After extracting with all solvent...What will v do..we will calculate % yield for all extract or what? Before proceeding or selecting extract for any activity....pls rply soon I have to do an activity but no idea abt solubility ...which solvent should i use for preparing extract?
@Dr.Rakesh.B2 жыл бұрын
You have to read literature for ur paper and discuss with your supervisor
@siddhip35882 жыл бұрын
@@Dr.Rakesh.B thnx for replying sir......but no sufficient review is available for the same....
@sangeethak81102 жыл бұрын
I am Extracting oil from raw leafy vegetables without drying and powder by using soxhlet apparatus. How to prepare sample for total phenolic content?
@Dr.Rakesh.B2 жыл бұрын
Consider the oil directly to be ur sample and proceed. But i don't know if this will work i haven't worked on oils. Try reading papers on oil or u can ask some experienced person in research gate.
@awaam395 Жыл бұрын
Sir how can we calculate the amount of herb needed for a certain amount of liquid extract? E.g. if we need 0.5 litre of extract for storage then how much herb will be needed for extraction?
@Dr.Rakesh.B Жыл бұрын
For 1 ml of extract usually 20-25gms of dry weight is required. Its all trial and error cannot be predicted.
@archanashukla7632 Жыл бұрын
Very informative video
@sonicarathore95057 ай бұрын
Sir plz reply how to use fresh sample for flavonoid content and phenolic content determination
@benjaminchanges7336 Жыл бұрын
Sir, Can I use this for plant protein extraction before using ultrasonication?
@nazishjameel-o2v7 күн бұрын
Sir I have evaporated solvent by using rotary apparatus. But now they become dry and get struck with plate. so how can I use them??
@Dr.Rakesh.B6 күн бұрын
Take 1 gm dissolve in 10 ml and prepare a stock
@NazishJameel-oy5yt17 сағат бұрын
Sir for how long time stock will use؟@@Dr.Rakesh.B
@shikharani49732 жыл бұрын
It is necessary to further dissole it in a methano? Cant we leave the compound undissolved in -20
@Dr.Rakesh.B2 жыл бұрын
You have to dissolve it in known volume of methanol so that you know the concentration. If ur experiments require crude extract in solid form then u can leave as it is and store it
@shikharani49732 жыл бұрын
@@Dr.Rakesh.B thanku sir for replying
@sarangabro3847 Жыл бұрын
Hello Sir, I am doing an experiment on a ethanol plant extract that I bought from a well known company. I want to check its antibacterial actions against some bacterial strains. I found no literature about the preparation of the solution of the commercially available ethanol extract of the plant. Therefore, could you please guide me as how to make the solution to check the antibacterial effects of the extract? Thanks
@vegetablescience83732 жыл бұрын
Can we crush sample in liquid nitrogen to make powder?
@Dr.Rakesh.B2 жыл бұрын
Such Lower temperature might affect the effectiveness
@lakykhatun71192 жыл бұрын
Sir is it okey if i use the liquid i mean methanol in which the grind sample pourend first?
@Dr.Rakesh.B2 жыл бұрын
Yes
@Xyz_curious2 жыл бұрын
1. Is the procedure sane for plain water extracts of plants? 2. Can these falcon tubes be used for Long term storage of extract?
@Dr.Rakesh.B2 жыл бұрын
Yes for both
@Xyz_curious2 жыл бұрын
@@Dr.Rakesh.B thank you sir
@Xyz_curious2 жыл бұрын
@@Dr.Rakesh.B sir wat about evaporating step? It will also be sam fr water extracts?
@Dr.Rakesh.B2 жыл бұрын
Yes since it's water it will take more time if you keep in room temperature. You can keep it at 40-50 degrees.
@Xyz_curious2 жыл бұрын
@@Dr.Rakesh.B what will happen if I skip the evaporation step?
@preetipatil63111 ай бұрын
Hello sir,may i know how to estimate the concentration of plant extract using UV double beam spectrophotometer?
@priyankakesavan5462 жыл бұрын
Thank you for the video sir. In my case I have to extract the chlorophyll-a from algae, so do I have to store the filter paper with solvent for 24 hours in the refrigerator before grinding it or should I have to grind and then store it in the refrigerator
@Dr.Rakesh.B2 жыл бұрын
Chlorophyll estimation is done by arnons method using 80% chilled acetone..Browse with this keywords you will find papers or in one video in my channel theres a video about references. Watch that video and download my paper for reference
@rajakumaris89095 ай бұрын
Super sir
@Akash_DC2 жыл бұрын
Can you please tell me the research papers which you followed make these videos like Preparation of plant extract, DPPH, TPC
@Dr.Rakesh.B2 жыл бұрын
There's a separate video in my channel for references. Please Watch that
@Mppscwithchauhan Жыл бұрын
Can i use normal filter paper ?
@Dr.Rakesh.B Жыл бұрын
Yes
@ruksanabegamchowdhury51582 жыл бұрын
sir i hv extracted volatile oil from hydrodistillation method now i want to dilute the oil in hexane for GCMS analysis...how can i dilute the sample can u plz guide me...i hv vry less amount of sample n i hv to perform further exleriment with that oil
@Dr.Rakesh.B2 жыл бұрын
For gcms 1:100 dilution also will work. It's highly sensitive. So dilute according to your other experiments. Just 10ul will be injected to gcms that's all
@ruksanabegamchowdhury51582 жыл бұрын
tnq sir
@titelmegu9632 Жыл бұрын
Sir, is it necessary for also vit c quantification ?
@easychemistryofficial2 жыл бұрын
Sir I rinsed my dried extract from rotavap flask with corresponded solvent and keep it in open air to dry the sample completely but it is taking long time what should I do? Ur response will b appreciated...
@Dr.Rakesh.B2 жыл бұрын
Use oven keep it at 40-45 degrees
@easychemistryofficial2 жыл бұрын
@@Dr.Rakesh.B Any type of oven means can I use microwave oven or not? Thanks 4 ur response😊
@Dr.Rakesh.B2 жыл бұрын
No you have to leave it for 2-3 days that's not possible in microwave oven. I had meant hot air oven
@easychemistryofficial2 жыл бұрын
@@Dr.Rakesh.B thanks🥰
@muskansheikh2629 ай бұрын
How much percentage of ethanol is used in this process
@nikshithajain252 жыл бұрын
Thank you brother ☺️
@upscziddi42282 жыл бұрын
Does Brahmi plant get extract by using hot water or cold water?
@Dr.Rakesh.B2 жыл бұрын
Refer from any published paper. It's not possible to know everything for everyone.
@nitinnangnurkar284011 ай бұрын
Bro pls explain how to make mother tincture ?
@nikkitaverma7354 Жыл бұрын
Sir for our project we need to prepare the methanolic extract of aloe vera gel. Can you please guide us on how to proceed for it.
@bhartirohilla3017 Жыл бұрын
How long we use crude extract for further activity
@easychemistryofficial2 жыл бұрын
Sir same extract is used for antimicrobial activity?
@Dr.Rakesh.B2 жыл бұрын
Yes you can use
@shilpasharma43834 жыл бұрын
Gr8 video sir
@sanjaykp10982 жыл бұрын
Is the method can be used for other sample? Means other than plant samples
@shreyakaushal82968 ай бұрын
Sir how can I mail you? I want to ask about one plant extract preparation for my master’s thesis sir
@gauravmishra21216 ай бұрын
How to take extraction from euphorbia plant
@ypdkm33403 жыл бұрын
Hello sir, can i use freeze drying to evaporate the methanol after filtration?
@anjanajayachandran68192 жыл бұрын
Sir for the extraction of saussurea lappa , is methanol or ethanol is good?
@Dr.Rakesh.B2 жыл бұрын
Please read literatures related to it.
@mohdyaseen45315 ай бұрын
sir could you please mention the article for references purpose. thank you
@Dr.Rakesh.B5 ай бұрын
I have made a separate video for references pls watch it
@priyamohan52552 жыл бұрын
good evening sir sir can we perform antibacterial activity of plant powder without any extraction if it is possible kindly guide me i m working on a herbo mineral preparation for my dissertation work
@Dr.Rakesh.B2 жыл бұрын
No, you have to dissolve it in some solvent .
@masoodahmedunar69712 жыл бұрын
How to do water exract of Chlorophytum Borivilianum Sufed Musli for human research use? plz answer soon I have to present my topic in interview
@Dr.Rakesh.B2 жыл бұрын
It will be done same way as this. You can watch other videos related to fungal extract preparation for more info
@anusihag162 жыл бұрын
If solvent is water.. how to obtain powder form of plant extract..lyophilizer or just evaporation in oven??
@Dr.Rakesh.B2 жыл бұрын
Oven will do the work. Just that a day or two extra time will be required.
@anusihag162 жыл бұрын
@@Dr.Rakesh.B thanks💫
@aartiAtkar Жыл бұрын
Can we use water as a solvent
@Dr.Rakesh.B Жыл бұрын
Yes
@dianahaobam8536 Жыл бұрын
Sir our guide let us make in 70 percent methanol....did u used absolute methanol means that 99.9 percent one?