AS Biology - How to calibrate a microscope

[@ahmedhassan-fo3hj]

Thank you so much first day of sixthform had biology and couldn’t get around the stage micrometer and eye piece graticule but your video has explained clearly and I can now get on with my homework

[@JoPhillipsBiology]

Im glad it helped!

[@benedicti1767]

Same bruh

[@ayesha1929]

same hereeeeee 😣

[@fahadijaz2292]

Thank you very much. I am a student at the university of Manchester and we need to learn how to do this as first year students. They failed to explain it but you have explained it perfectly here. Your teaching style is perfect!

[@mychemicalreaction73]

BEST EXPLANATION SO FARRR

[@Sophia-ok4ce]

Usually only look at the big subscriber KZbin channels for this like free science lessons etc but this is the only video that’s helped and I fully understand thank you!

[@JoPhillipsBiology]

That's fantastic to hear, thank you for letting me know. Please subscribe if you haven't already, and tell your friends! :-)

[@bouchoswatz]

Hi Jo, I believe you added an extra '9' in your conversion to µm with the 4x objective, as you had the 10 e.g.u. equal to 0.99 mm, not 0.999 mm. So the result should be 99.0 µm per epg unit not 99.9.

[@mairaasim7487]

Yes Exactly

[@JoPhillipsBiology]

Ah I see, I'll check and correct it if needed, apologies.

[@rekikaknaw4547]

Thank you so much!! This changed my life!

[@anythingajag]

Nicely explained - have now calibrated my microscope - Thanks!

[@shovasupakar8268]

Awesome explanation. Hats off to You. Madam

[@fandisatia]

your explanation is detail and easy to understand. thank you very much.

[@anastasiiatokareva5434]

What a helpful video! Thank you very much for your clear explanation)

[@JoPhillipsBiology]

Glad it was helpful!

[@miranirb]

Great video and it's very clear! But I have one question from the past paper that I still don't understand. If it's possible, where can I contact you? Thank you very much

[@Jophillips36]

Hi. I’m glad you find the video helpful. You can ask me your question here, or you could private message me via Twitter - @jophillipsbio

[@wazinahirwin6822]

u made my day, da best!

[@felix1705]

perfectly explained, thank you I appreciate this !

[@Vain-Voyager]

Thanks for sharing! Very clear. Just wanna know why don't we have an objective graticule which comes with a given objective? In this way, wouldn't it be easier to calculate the item's size without calibration?

[@JoPhillipsBiology]

Hi. That’s a good idea and to be honest, I don’t know. I assume it’s something to do with the way microscopes are made which prevents a graticule from being placed in the objective lens. Could also just be because lenses can be swapped in and out of a microscope, so it might just be too expensive to put graticules in all objective lenses.

[@Vain-Voyager]

@@JoPhillipsBiology May I know the reason why it is 99.9 instead of 99.0 @7:30? Thanks!

[@itsjsyall]

this video was so helpful , thank you :)

[@rogelindabarraza1993]

Thank you so much for this explanation!

[@JoPhillipsBiology]

You're very welcome! Please subscribe if you haven't already :-)

[@FanFridaDuncan401]

Hi! Great video! I have a question, I see that all this calibration is made every time I need to use a different objective, 4X, 10X, etc But my question is if I need to calibrate again if I change the sample thickness, since I move the coarse and fine focus to focus the right layer. This question does not let me sleep. thanks!

[@joshuaservidad4612]

In 6:58 why is it 10 e.g.u not 100? Is one egu per one big division?

[@Fatima_567]

thank youuuuu

[@piece9382]

Hi! Does this mean that the higher magnitude, the more precise we can measure the cell’s length? (since it’s smaller?)

[@JoPhillipsBiology]

Sorry for not replying sooner. Do you mean the higher the magnification, the more precisely we can measure? Yes, you will be increasing the precision, but only because the eyepiece graticule scale becomes finer. Precision is to do with the measuring instrument you use (how small are the divisions).

[@mojam6715]

Thank you, this was very helpful

[@JoPhillipsBiology]

Glad it was helpful!

[@karolinapefinde6737]

Hi... Thank you so much for this, it's super helpful. But I kept on getting different answers from yours though.. maybe there is something wrong

[@0308.]

Thanks for this video

[@renujangra0344]

Thanks sir it was really very helpful....

[@BohorquezL]

Hi Guys anybody knows if there is a way to calibrate my dissecting microscope if I dont have a stage micrometer, can it be donde with a plain ruler ?

[@rosaliatweufiilwa1066]

thank you..... this was helpful

[@JoPhillipsBiology]

Glad it was helpful!

[@abdulqadirmaricar4596]

Why can't you use use the stage micrometer to measure it?

[@ABrian]

Because you have to take the stage micrometre off the stage to put the specimen on.

[@hongjoongswife9672]

6:02 I’m so confused how is the distance on the stage micrometer 0.1 mm, doesn’t it increase by one

[@user-eb6he9xq2v]

Hey I have a question Why did you write 10 egu = 0.99 mm instead of 0.9mm?

[@JoPhillipsBiology]

Hi. Sorry for the late reply. I think I must have been working without rounding? Tbh I’m not sure but I’ll go back and have a look. It shouldn’t make any difference to the understanding of the concept and calculations though.

[@mr.sharky3031]

Hello, thank you for the video. It was very helpful. At 11:23, I chose to do 6egu = 2.3mm and the answer I got was 1egu = 0.383 mm, but you got 0.038mm. Can you tell me why my answer is wrong? Thank you:)!

[@JoPhillipsBiology]

Hi. I'm not quite sure what you mean. When you say mm, do you mean micrometers? And when you say you chose to do 6egu = 2.3 mm, where did you get this from?

[@user-iq5tl1jk4w]

Thank you!!

[@ramsha-ky5fc]

Thank you!!! 🙏🏻

[@itztigger1373]

How do you know that each division is 0.1 mm on the stage micrometer??!

[@JoPhillipsBiology]

If you do this in real life, you would look at the stage micrometer and it will tell you. This is because you can get stage micrometers with different scales. In my video, I used those divisions as they tend to be more common. In an exam question, they would also tell you the size of the divisions (so don't assume it's always 0.1 mm!)

[@taalyelxor]

Hi there, I have just found your channel, could you please tell me if your content follows Cambridge international Examinations or another exam board? Thanks

[@JoPhillipsBiology]

Hi Alex, I'm so sorry for not replying, I must have missed it. Yes, everything is CIE. The biology itself will obviously be the same as for other exam boards, but the level of detail and emphasis may be different.

[@taalyelxor]

Cheers! Thanks for that!

[@aimantariq6377]

Very useful 👍👍 Thanks!!!!

[@JoPhillipsBiology]

You're welcome!

[@avocadowithice3426]

I don't get how you calculate

[@ainharamk550]

7:01 why is it 0.99, isn't 0.9? how would i assume that there's numbers after the 9

[@JoPhillipsBiology]

It's just a case of how many places you round your answer to. If you do the calculation, you should see that I have rounded to 2 decimal places.

[@ailtonsouza5162]

How scales are recorded in a microscope reticle?

[@JoPhillipsBiology]

I'm not sure what you mean? The eyepiece graticule scale is different, depending on the objective lens you are using. That's why you need to calibrate it using the stage micrometer.

[@hellokitty4966]

Thank youuu

[@rustamshekh9826]

Thank-you so much

[@JoPhillipsBiology]

You are so welcome!

[@hanyabdulla8113]

Thanks 🙏

[@ndinelagotulela569]

How did u get 0.99

[@ayeshaazam.1508]

thankyou

[@candoschool1975]

great

[@rustamshekh9826]

Help full video . Good work .

[@JoPhillipsBiology]

Thanks

[@Hello-ln7zi]

Please answer me! How the 1 e.g.u= 0.099 mm???!!! And from where you you got the 99.9mm I don’t get it!

[@Hello-ln7zi]

0.099 supposed to be the final answer fot the 1e.g.u right!?

[@JoPhillipsBiology]

Hi. You work out the length of the eyepiece units by using the stage micrometer. When we lined them up, 10 eyepiece graticule units matched up with 9.9 stage micrometer divisions. We know each stage micrometer division is 0.01 mm. 9.9 x 0.01 = 0.099 mm. So that’ the length of 1 eyepiece graticule unit (1 egu). There is no 99.9 mm, you have the wrong units. I converted 0.099 mm into micrometers (1 mm = 1000 micrometers). Watch the video again and try to follow through the explanation again.

[@Hello-ln7zi]

@@JoPhillipsBiology I got it now thanks alot🥰

[@Hello-ln7zi]

@@JoPhillipsBiology I got it now thanks alot🥰

[@qwertyytrewq9873]

Why 0.038mm is equal to 38.46 micrometers? Why not 38?

[@JoPhillipsBiology]

I think I just rounded when I wrote it as mm!

[@bushramaqsood5841]

Plzz explain this lecture in urdu also plzzz

[@61814_]

I love you❤

[@welln0w]

still can't make sense of this

[@barley8089]

stop putting so many ads

[@editsofstuffilike._9406]

What ads? Lol

[@monkeyfat6446]

leng video