Aseptic Technique and Liquid Cultures: Trouble shooting problems and how to grow mushrooms with LC

This is a deep description video of LC's or Liquid Cultures and mushroom cultivation - ENJOY
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[@Fungipedia-IN]

I'm already a decent cultivator and a graduate of microbiology, but I must say I learned a lot from your videos. Thank you so mush :)

[@Subs1338]

He seems to know a ton and has a lot of good info but its sometimes hard to get through a video cause he talks slow, repeats himself alot and is verbose.

[@HeyRaeSunnyDay]

Wait what, did you guys have a baby? (Based on your t-shirt) wow, congratulations and welcome back Garry, we missed you!

[@MasterShibo]

Vert informative and professional video! I purchased the jar lids, amazing!

[@FattyFelix]

Gary, love your videos, long time watcher, first time commenter. This has really helped and stopped me caring for a culture that looks nothing like my others where they literally cloud, this one grows but is flakey like the one you show here, so thank you! Really appreciate your videos, you cover so many subjects that are not found elsewhere. Would love to see more fail videos, not that I wish you bad luck! Seeing fails and helping us all recognise them is just really helpful. Wishing you love, happiness and prosperity! looks forward to your future vids! Baz, Scotland 🏴󠁧󠁢󠁳󠁣󠁴󠁿

[@skyelord6229]

I like those printed labels you use. I've peeled them off the syringe and stick them onto my LC lid. Good to see you!

[@syberphish]

Gary I'm so glad you're back!! A few times now I've thoroughly sprayed my syringes with iso only to realize the labels bled off... 🤣 It's so nice to know I'm not the only one who's done that. Had to try to remember which was which. A piece of scotch tape over the label works pretty good.

[@MarcCGN]

Get some Edding 780 pens, they're alcohol-safe.

[@bounceurabdelaziz4973]

Thank you very much Gary for your sharing, my greetings to you from Algeria🇩🇿☘️☘️.

[@spicytrinirice]

im sooo happy you are back..always a pleasure to watch a new video from you

[@noblefungifarm599]

Hi Gary, this is an outstanding summary video. You have covered about 5 different vids worth of info. Thank you for your generosity kind Sir.

[@MagicinalExplorer]

Gary! You’re awesome, keep rolling out the mind blowing content 🪶👁🍄

[@ZCRAIZED1]

The cheap Temu thermal printers and labels work incredibly well. 12 to 15$ for a Bluetooth printer and 10$ for 1000 labels. I spray alcohol right in them many times and rhey dint change at all. I soaked one in alcohol for a few minutes completely submerged and it lightened but did not disappear.

[@jaysonramsay3896]

This is very helpful. LCs are amazing, a few threads of mycelium expands to a snow globe

[@M00nBeard]

Your 16gauge needles are so much better quality than what I’ve been getting. Out of curiosity where do you get those?

[@Chloekat1234]

I started an LC with a spore syringe successfully. It works well. But better if you have mycelium to start.

[@TechGorilla1987]

Funny story - or not... I made an LC and it looked great, but got a dark spot in it - then two. It was made from agar not spore. I made 4 more LC jars and let them rest in the pressure cooker before using. All had growth without being inoculated. What I had in common with all 5 was using a decorative polished rock (boiled and pressure cooked) as a stir mechanism. Obviously, the rock was the source of contamination as I made LC with glass beads previously without issue. I have two more new ones with boro3.3 stoppers as stir bars. I hope this goes better.

[@kartalbaba981]

Thanks for this great video.

[@mahmoudbamarni3803]

thanks for this information

[@HBaum-qg5fy]

Hey Gary, once you showed LC with bakterial contamnation. I did not (accoustically) understood what you are doing to clean these glasses up ?? Thankful greetings from Germany !

[@FreshfromtheFarmFungi]

wash them out with soap and water and let dry, some people autoclave them but it’s not necessary

[@HBaum-qg5fy]

@@FreshfromtheFarmFungi TNX ! You mean the glasses? But can I save the mycel or is it gone? Thinkt, it is gone and over, right?

[@ranjanty]

The shirt is the coolest ever. It probably was a gift but would you happen to know where they can be purchased? As always love your work , thanks.

[@FreshfromtheFarmFungi]

Thanks! My friend got it for me here: tidd.ly/3jKlmUB

[@jakestephens4868]

Going to try your Piopinni and Wine Cap cultures soon. Love the vids, some of the best info (esp on breeding) on youtube. What generation are your LCs? Plan to use each LC to make a grain "master" and g2g for spawn and just want to keep track of the generations.

[@FreshfromtheFarmFungi]

Nice! They are first or second gen off of the slants 👍 I usually start my king stropharia in Feb

[@TechGorilla1987]

@9:47 - What sort of LC would have darker spots in it that semi-resemble spores? That example that you show has them, and you advise that looks healthy. Could you elaborate please? I have an LC (the one from the rock story) that looks somewhat like that example but with only 2 darker areas.

[@craiginboro679]

Bit worried about how sterile your environment is, see video of flying insect from 5:35

[@FreshfromtheFarmFungi]

good catch, rogue fungus gnat which can cause a lot of problems but we shut down our grow and are moving to the new spot

[@InconspicuousOwl]

Very tasty!

[@skiddysm]

You can easily make your own self healing injection lid for cheaper and no need to buy replacements you can just repair or make more very cheap and easy! Also don't use mushroom spawn bags use the jars for mushrooms spawn you can do this as a beginner all the way to semi commercial! Only use bags for commercial growing...

[@flowleopard893]

Bags are ideal for people with a small pressure cooker. By weight a lot more spawn will fit. Also, glass is expensive. Running consecutive PC cycles with low efficiency is expensive. I agree on making one's own lids but bags are not a bad idea for beginners.

[@akashicrecord2120]

Can you please share a link to the vendors selling the IV LC setups that are similar to the one you said you bought at Lions Mane in Denver?

[@FreshfromtheFarmFungi]

amzn.to/3X3mNfp Amazon link

[@FreshfromtheFarmFungi]

amzn.to/3X3mNfp @mushroomchamp here in Denver

[@FreshfromtheFarmFungi]

amzn.to/3X3mNfp @myersmushrooms version

[@5280fame]

How many cc do you use to start a lc in a jar?

[@nelsonwelser116]

Hello, here from Uganda, in Dubai now. Qn is, how long can that LC syringe be kept? and which method is best for long term storage of mycelium

[@FreshfromtheFarmFungi]

best method is with a slant like here: kzbin.info/www/bejne/e3-ufHh9l6lkiLs but lc’s can last months or even years

[@tedkravos7129]

If you fill a jar with 2 LC's together, does that create a new species of hybrid mushroom when it grows mycelium?

[@eyesdontsee]

No

[@Chloekat1234]

No. But you can cross strains among the same species. At least with p. Cubensis 😉

[@Chloekat1234]

But you do it with spores. Not mycelium.

[@carloslao6374]

Do LIquid cultures need to be in the dark for the mycelium to grow?

[@FreshfromtheFarmFungi]

no they will be fine just out of direct sunlight as UV can damage the mycelium

[@zachsonpub]

Hey when you PC these myco lids (with the syringe filter/SHIP) do you foil them?

[@FreshfromtheFarmFungi]

no

[@zachsonpub]

Nice. I have no experience with these filters so wasn’t sure. Thanks Gary

[@ScottYdo]

How long can mycelium last on a petri dish sealed and put in the fridge ? How long can LCC last stored in the fridge?

[@FreshfromtheFarmFungi]

both about the same (6-12 months are best - it may very strain to strain and as long as neither dry out or get contammed or freeze it can be much much longer (decades))

[@Chloekat1234]

@@FreshfromtheFarmFungiyou can freeze LC syringes? I deal with P cubensis only. And it’s a tropical species- wouldn’t freezing kill the mycelium? I know heat over 105-6 F will kill it. I’d assume freezing would too

[@Mycoraiseall]

Good sir.. what size stirbar is best?

[@FreshfromtheFarmFungi]

it depends on the container

[@antonanon]

Hi Gary, what may I ask is your stirring regime?

[@FreshfromtheFarmFungi]

I usually stir for a few minutes at a time once a day

[@antonanon]

@@FreshfromtheFarmFungi Thank you for this. My poor LC must be dizzy AF as I’ve been stirring overnight! This has been causing what I believe are mycelial micro fragments to create some turbidity & mimic contamination; which subsequent quality testing has negated. I wonder whether severely agitated agar sections can also cause some turbidity. From what I’ve observed bacteria or mould contamination remains suspended in the LC, whereas, the agar & or mycelia micro fragments settle out. Cultures which have had excessive agitation form, jiz like colonies at the bottom, whereas less agitated ones are more distinct confetti like segments.

[@RustyColonsCPA]

@Gary how long do the syringes last in the fridge?

[@FreshfromtheFarmFungi]

months to years it depends on strains, conditions, contamination control etc. but at least 6 months if maintenance is done correctly

[@RustyColonsCPA]

Thank you!

[@RustyColonsCPA]

No sound

[@ShowMe78]

Has sound on my end

[@triple_gem_shining]

You have NO SOUL!

[@RustyColonsCPA]

Mine is working now

[@RustyColonsCPA]

@@triple_gem_shining nobody does smarty pants

[@syberphish]

Gary... are you gonna be a dad???? Dude I just read your shirt.

[@FreshfromtheFarmFungi]

yes we had our first child December 1st! 🙏🏻🍄❤️

[@syberphish]

@@FreshfromtheFarmFungi I'm so stoked for you guys!!! ^__^ you're both going to be amazing parents. Congrats!!