Mycology Lids Explained: Full breakdown of liquid culture lids and back engineering media extractor

[@arcies9286]

People who donate experience and information are just as generous as those who donate to charity or the needy. This is one of the mushroom cultivation "treasure channels" that gives much and demands nothing but the time spent learning in return. Thank you for this and every other video you've shared inspiring aspiring mycologists o/

[@SarahStone-l2x]

at first i wasn't sure about your content but i'm sold, every time i have a weird question your videos pop up with the answer...you rock 😁

[@chicofromph33nix64]

Same... I was searching everywhere for the purpose of the syringe filters and nobody talked about it. So I would ask in the comments and nobody would give an explanation. Found this channel and this guy has already answered 3 of my questions in 2 videos.

[@mycologylids]

Very informative and detailed breakdown. Your passion for this subject is really evident in your explanations. I appreciate how you took the time to walk us through the different types of lids and their respective benefits. Mush love! EDIT: The tubing inner diameter is 1/4" and the outer diameter is 3/8" (for the master LC lids)

[@BarackObamaJedi]

I made diy lids with silicone and micropore tape, works wonders, but i never thought about how the hole is needed for air displacement. Great insight!

[@LeonardoPeSe]

Dude your videos are amazing, have learn a lot, I miss your content.

[@natishamullis-brooks5715]

Great video! That was a small mouth quart jar, in case anyone wants to know.

[@SaltyJuan]

Thanks for the video Gary! Been meaning to modify lids for LC etc for a while, so this is a calling! Mush love from Australia

[@SPOREdotSTORE]

Awesome!

[@nickdeborbon4947]

I make my own. And for my lids. One 5/32 and one 7/16 drill bits are perfect sizes for my filter and injection ports. No adhesive, just a very tight press in seal. I just pull out the filter and SHIP when I think they need replaced. It's so easy to do It doesn't feel right selling them.

[@FreshfromtheFarmFungi]

thanks for the info! You’d be surprised how many people can’t use a drill or just don’t have the time/patience 👍🍄❤️

[@jbooger13]

How do you keep the end of the piping sterile? Especially if you cap it off with a syringe or leur lock cap wouldn't any lc remain in the end section? Wouldn't it be preferrable for it to run back into the jar? Thanks.

[@lornemosley8892]

Loved the video! This will help step up my liquid cultures. Thank you so much for all the content, it's become my after work ritual to learn from your videos.

[@FreshfromtheFarmFungi]

thanks for watching and following along!

[@DaxPegels]

Yes! I have been waiting for this video. Been working on more LC and wanted to make an extractor lid. I can source all the lab stuff pretty easily

[@Alisha-wr3os]

GARY!!! josh here (again) in Colorado springs.. you helped me find my flowhood (modular cleanrooms) and you were gonna come to my lab for some consulting.. Quick question for ya gary.. i have a tea business and i want to get on amazon but im not sure what app to use for my product thumbnails.. all of your thumbnails always look so awesome.. any advice on what apps to use? thanks again man.. ive been a subscriber and admirer of you for a few years now..i was subscriber 4,000 ish and im always sending my friends to your Etsy for gourmet strains etc..

[@FreshfromtheFarmFungi]

thanks for following along! I just used microsoft word and imovie with my iphone - keep it simple and easy! 🙏🏻 I have an Imac for video editing and my macbook for all the thumbnails, titles etc. People on Fiverr helped me a lot in the beginning and then it took a lot of practice to get honed in. Some of my earlier ones are still up and quite different then the style now - MUSHLOVE

[@irate74]

@ 10:23 That obnoxious bend in the tubing ends in the middle of the jar so you'd essentially only be able to draw half of your LC through the tubing. You could get the last half with a syringe but that defies the whole point of using the tubing imo

[@CamxCam.]

The length was for a quart jar, & I'm sure he was just using the half quarts as a demonstration.

[@ScottWConvid19]

Excellent content, as usual. Have you got an update on the morcella garden? Also, which glass pint or quart jars work best with the magnetic stirrer? I bought a case from my local garden center and they have a fairly exaggerated concaved bottom which sucks because I basically used all my culture on them to learn that the magnetic stirrer will not work with them

[@FreshfromtheFarmFungi]

nothing popped yet 😬 it’s a half pint widemouth ball jar try getting a smaller stir bar that can help too

[@Jack-pi2lz]

Great stuff. Thanks for the breakdown Gary

[@jimycrackcorn408]

yo Gary, what’s up with discount code for master LC lids? I watch your videos, religiously and over and over and over again whenever I need a reference, but I find a whole world of useful information from you and your company I appreciate all your hard work. I appreciate you putting this information out there for all of us to view and learn from I appreciate 🫵Thank you.🥰

[@FreshfromtheFarmFungi]

thanks! I don’t know of a code for the lids but I have some listed on ETSY and our ETSY code is FungiFam303 for $20 off any order over $120 etsy.com/shop/freshfungi 🍄❤️

[@mycologylids]

Use code FRESH10 for 10% off. Code is valid until 31Dec2024!

[@johnnyfish6051]

How long would you say morchella Americana spores would remain viable in a dry climate controlled environment?

[@shaqx7039]

Where did you get your laminar flow hood from?

[@FreshfromtheFarmFungi]

we got one from air science and one from myers mushrooms

[@louisw5858]

Small question:- while the culture is being preserved in fridge, is it necessary to do fresh transfers every 2-3 months as they run out of nutrients??

[@josephconkwright4215]

I've had a culture of Nepal chitwan that made it in room temperature for two years so in the fridge it should be fine for at least longer than 3 months

[@louisw5858]

@@josephconkwright4215 so what is this transferring about?? I hear it often

[@josephconkwright4215]

@@louisw5858 it's taking a s syringe and sucking some mycelium out of one liquid culture and then innoculating another jar of fresh liquid culture such as honey water or corn syrup water with the syringe.

[@josephconkwright4215]

You can transfer to be safe and that's better but I don't think it's necessary as often as one might think

[@FreshfromtheFarmFungi]

it’s not necessary the culture will regulate itself for a while

[@baroncastanares2469]

Hey. I had a question about the advanced lid with the clip to prevent back flow. 1. How do you extract a full syringe of LC when there is air in the tube? When I try it only fills about 4cc 2. When you snap the clip to close the tubing, how do you expel the liquid in between the syringe and clip to prevent air in the system and prepare for subsequent LC extractions? Thanks!

[@FreshfromtheFarmFungi]

you can either ad a backflow preventer or just keep the hose kinked between syringes. Also, I will leave the last syringe in use on the end as a “label” and as a cap to keep air from entering the system. Things have calmed down a bit here I want to make a recap video soon

[@dantepicantemartinez]

I would love to see I recapping vid. I bought the master Lc lids and gave the jar with the lid bacteria. Im not sure where I’m the process I mess up but I’m sure it’s user error on my end.

[@Chris-ru5hb]

How do the mycology lids tubing not get contaminated after pressure cooking?

[@FreshfromtheFarmFungi]

if it’s already connected and capped off it will be sterile until it’s opened and then it’s a matter of doing proper aseptic technique

[@johnhuynh248]

Can the syringe displacement port sterilize ?

[@levittperez]

How would you sterilize between uses for the master lids with tubbing?

[@DJ-xo4jg]

I'm sure your aware of the mono tub tek, and flushes. How do you feel about using grain water for misting and rehydration for flush?

[@mybigsteaminjohn4027]

No.

[@DJ-xo4jg]

@mybigsteaminjohn4027 why? Isn't that what the fungi thrives on? That's why it grows in dung, it's the grain the animal was eating that supplies the food source.. or am I wrong? No. Doesn't answer a fucking thing, much less the original question.. now if you're nothing but a douche, well that's easy to see now.

[@a23meme]

does the pipe get contaminated while storing? since we're using the same pipe to connect syringes multiple times ?

[@FreshfromtheFarmFungi]

this is the caveat - I keep a bunch of spare tubing sterilized in pouches and if I suspect this, I just swap for fresh tubing. The key is to never have the liquid go back to the main container that way there is a break point you can just swap between pulls

[@nicolemichele-darkwoodland2136]

which way do you have the filter facing in the lids and why that way?

[@Mechaneer]

The one with the clamp...doesn't solution get stuck between the clamp and the tip where you insert the syringe, and then spill out when you pull the syringe off?

[@FreshfromtheFarmFungi]

no, there isn’t any back pressure the clamp acts as a backflow valve

[@jeremekrug458]

Have u posted links for the parts to make the lc lids

[@FreshfromtheFarmFungi]

they are on our amazon affiliate page

[@edarrenellis]

Gary, some of the parts on your list seem to only have a temp rating of 175F. Have you found this to not be a problem?

[@FreshfromtheFarmFungi]

it shouldn’t matter unless it’s tubing, don’t use vinyl tubing but what I have listed is what Ive been using and it works great

[@م.مهاابراهيم]

I need your help please Haw can I prepare bromothymol blue stain?? I have the powder of bromothymol blue

[@GNARLOUSE]

Thanks I needed this. My idea (what I'm presently using) of the early days is the original jar lids I modified with hobby lobby felt and silicone. I'm assuming all of the components of these lids are vulcanized right?

[@FreshfromtheFarmFungi]

Im not sure but they lasted for 2.5 years

[@GNARLOUSE]

@@FreshfromtheFarmFungi nice, thanks again!

[@jhonnewton7433]

5bl Grain spawn bag is how many can use 5bl bags for block?

[@jgarcia9734]

What about aerating ur liquid culture with an air pump and filters. Almost like brewing a compost tea.

[@fancycrafts7774]

Why though?

[@FreshfromtheFarmFungi]

yes I have done this and am planning a system to scale it, there is risk with contamination but it helps grow it fast

[@FlatcapMushrooms]

Hey how are your planted morels doing this morel season do a video please we live close in proximity and I’ve been trying new things too

[@FreshfromtheFarmFungi]

still waiting 😬😬😬

[@cestlaviehappner1872]

So do you still wrap up your tops with aluminum foil or do you just let's set them in there like the ones for the syringes

[@FreshfromtheFarmFungi]

no foil needed just be careful to not pull them by the tubing and it works well

[@cestlaviehappner1872]

@@FreshfromtheFarmFungi so I'm assuming the syringe filters will be just fine into

[@Shroomaholics]

Do you know what type of tubing is used for the lids?

[@FreshfromtheFarmFungi]

it’s lab grade silicone I believe

[@thekhibros7388]

So you can PC these with the tubbing?

[@mycologylids]

Yes

[@MycoDogs]

i was awake this morning

[@FreshfromtheFarmFungi]

I have to wake up before the baby to do these now hah

[@archiewebster5034]

Great video, really helpfull

[@FrankEmmanuel799]

I bought some growing kits, spore prints, spawns, liquid culture and psychedelic products from an online supplier and they are affordable and discreetly delivered

[@FrankEmmanuel799]

FROM TRIPWITH_JEFF

[@FrankEmmanuel799]

in instagram

[@christod.1061]

Thanks!

[@bill6943]

Just ordered some Porcini LC. I have not been able to find it anywhere in Australia. Has be be the most I have ever paid for an LC. AU $68

[@FreshfromtheFarmFungi]

it is an aggressive one - I am planting under some douglas fir this year

[@MJ-bg8gn]

Very good!

[@Zushi1312]

Love the vid! Unrelated question but do you have any thoughts, opinions or any hot-takes on why Paul Stamets wouldn't comment on the portabelo mushroom industry in his latest apearence on JRE ? Personally I I think he contains a dogwistle in his response for myco nerds saying in passing "its a very explose issue" from my very limited research I think its because portabellos contain a compound 'ageritine' which, apart from being carcinogenic can be used as an ingredient to make 💥?!? Pauls response to joe was really weird and exeptionally jarring. I also just cant believe that the gormet mushroom industry has 😴r agents ready to take someone out just at the mere mention of the potential issue but then again maybe im blissfully naive 🤷‍♀️ As i say i havent done a lot of research but though I would ask yourself as you're wayyyy more clued up on this whole sphere on knowledge than myself lol 😆 so yeah... TL;DR : Why are portabello mushrooms bad Gary ?

[@salovamrani2084]

How to sterilize injection ports?

[@FreshfromtheFarmFungi]

rubbing alcohol

[@salovamrani2084]

@@FreshfromtheFarmFungi is it goid enough? I read on the forums its not 100% reliable

[@jbtravelssolo7596]

you do realize the back of those syringes are open and exposed and then you aspirate back and forth you are introducing contaminates right. 8:37

[@FreshfromtheFarmFungi]

Yes that is the whole point to displace the air while aspirating and filter it through .2 microns did you even watch the video?

[@Alex-gb4vn]

The problem with putting a syringe on immediately after inoculation is that the majority of the syringe is exposed to open air until you decide to take your first sample.

[@VideoMatti90]

I think mycolids is not selling these lids anymore...do you any other valid source to get them?

[@FreshfromtheFarmFungi]

they may just be sold out

[@FreshfromtheFarmFungi]

also, check the amazon page out all the parts are there to build your own

[@VideoMatti90]

@@FreshfromtheFarmFungi yeah i've seen these,but seems not that easy to build

[@xMrSilver99x]

find their origin at Spencers Shroomery

[@graceyou237]

@@xMrSilver99x they never ship, tried to order last month

[@MrIzzy11B]

That’s nowhere near a half a gallon jar 🤣🤣🤣🤣🤣