Basic Mechanisms of Cloning, excerpt 1 | MIT 7.01SC Fundamentals of Biology

Basic Mechanisms of Cloning, excerpt 1
Instructor: Eric Lander
View the complete course: ocw.mit.edu/7-01SCF11
License: Creative Commons BY-NC-SA
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[@johnasd4685]

You are an excellent lecturer. Hope your students understand your great value.

[@rosacosta4091]

I wish this lecturer was my teacher, he is very skilled

[@hamidkiangaikani]

@@alejandrodelabarra2838 Wrong! Not yet

[@alejandrodelabarra2838]

@@hamidkiangaikani Sorry! I was misiformed... He deserves it.

[@hamidkiangaikani]

@@alejandrodelabarra2838 Totally. He's my super hero

[@juliocoutinomontes7978]

the best lectures are those that includes stories of the origin of the things, this man is a great teacher

[@dianamaeponce5847]

This professor is so great! The definitions doesn't come straight from him but from his students. He uses the art of questioning and humor to elicit responses from the audience and build up understanding of the lesson. Excellent!

[@Togetherness23]

I love the energy he has makes me want to learn more and more and more ! #biomajor. The 21 people that disliked this , need to find a new major.

[@alejandrodelabarra2838]

They don't have the money to pay 5 seconds of his lecture...

[@NomeCultJoe]

Update: Ecor1 in 2018 costs $60 for 10,000 units. This is $40 less than the 2012 price of $100 for 10,000 units.

[@surat8591]

Excellent 👌!! Great lecturer!! Engaging, passionate and personable. Those MIT students are so lucky to have a scientist like this as their professor!! None of that unfounded arrogance you see from other professors who are not half as good as this man.

[@ta-tahuang4904]

Best introductory lecture ever

[@valle5169]

I wish I could have this lecturer. Thank you for sharing this content. I really needed it.

[@veeraselvan1956]

Very excellent teaching l like that Eric Lander sir very great !

[@bichngoccaotran1109]

I love professor Eric Lander!

[@kundhan829]

i am too

[@puekai]

Cleared many of my frustrations in this topic.

[@CaptainVelveeta]

This is the only guy I've come across that teaches exactly like my Professor...with nitro base sequence charts instead of elaborate drawings.

[@alejandrodelabarra2838]

Simply Outstanding.

[@MohammedAmodu]

waw! so, interesting . Really , your are a great scientist

[@puekai]

Amazing teacher!

[@Abdullah-vx7dx]

best lecture I have ever heard in my life

[@deebharath3940]

This why I love molecular biology just fall in love with that this lecture too good thank you sir nd MIT

[@HafizahHoshni]

16 Basic Mechanisms of Cloning, Excerpt 1 (00:13:19) Thanks !

[@wasiqamin]

0:14 step 1 cut DNA 1:41 palindrome 3:55 E .coli 4:48 Frequency e.coli 5:45 why isnt e.coli chopping up its own DNA 8:04 e.coli imune system 9:23 restriction cells 9:39 another restriction enzyme 10:09 another guy 10:28 another 10:40 another some

[@AlonsoRules]

this dude explained a whole lecture series in 10 minutes

[@bharath1397]

Super explanation.

[@muffinman12269]

It turns out this guy is amazing

[@dilpreetsingh7645]

Very helpful

[@KSATica]

So the 4 he got is from AATT or TTAA then the 6th power he got from the letters then 4 to the 6 is 20000 is number of possiblities of where it can land.

[@alejandrodelabarra2838]

Thanks for giving us a lecture we could never afford!!!

[@jasonjones2064]

This doesn’t have much to do with cloning ....... still...... something to consider....... The 52 amino acid host killing peptide (Hok) from the hok/sok post-segregational killer system of the Escherichia coli plasmid R1 was synthesized using Fmoc (9-fluorenylmethoxycarbonyl) chemistry, and its molecular weight was confirmed by mass spectroscopy. Hok kills cells by depolarizing the cytoplasmic membrane when it is made in the cytosol. Six microorganisms, E. coli, Bacillus subtilis, Pseudomonas aeruginosa, P. putida, Salmonella typhimurium, and Staphylococcus aureus were exposed to the purified peptide but showed no significant killing. However, electroporation of Hok (200 microgml(-1)) into E. coli cells showed a dramatic reduction (100000-fold) in the number of cells transformed with plasmid DNA which indicates that the synthetic Hok peptide killed cells. Electroporation of Hok into P. putida was also very effective with a 500-fold reduction in electrocompetent cells (100 microgml(-1)). Heat shock in the presence of Hok (380 microgml(-1)) resulted in a 5-fold reduction in E. coli cells but had no effect on B. subtilis. In addition, three Hok fragments (Hok(1-28), Hok(31-52) and Hok(16-52)) killed cells when electroporated into E. coli at 200 microgml(-1) (over 1000-fold killing for Hok(1-28), 50-fold killing for Hok(16-52) and over 1000-fold killing for Hok(31-52)). E. coli cells electroporated with Hok and visualized using transmission electron microscopy showed the same morphological changes as control cells to which Hok was induced using a plasmid inside the cell.

[@vickyoli]

I really like this professor Lander. But, who got the CRISPR-Cas9? ** drops microphone **

[@KSATica]

And does it matter what sequence. In other words the re-arranging of the letters. And I like this teacher but aren't there more clear-cut explanation you know short and sweet way to explain. Gracias.

[@KSATica]

I agree :)

[@anilkumarsharma1205]

genome mixing of all types of oil plants together will gives a complex compound for fractional distillations and patrol solution become easy forever

[@user-ps9fd8sz4g]

制限酵素だ　懐かしい

[@KSATica]

So basically what Eric Lander did was once he found out the first ladder I assume then flip the second and reverse it. I'm not so sure if its true. I'm just guessing here.

[@KSATica]

It's only pretty cool if we can experiment mutiple of times and apply the formulas to the test lab.

[@ammarahmedani601]

Why not bacteria do the same with acquired gene e.g. gene of resistance acquired fro other bacerium ? Is it cleaver to the extend?

[@SebastianRodriguez-vf5um]

Probably because that gene it is methylated too.

[@joedart8449]

@@SebastianRodriguez-vf5um So that new gene's methylation is matched to the bacteria it will be inserted into?

[@KSATica]

Plus I do apologize with all of these series of question but I'm a student who is kind of curious to learn pretty much that sounds like a challenge and have something to do with science or mathematical formulas. :) Lo siento. But hey I rather be doing this then writing a book. Playing with letters and numbers don't sound difficult. It's child's play to me once I understand why.

[@Abeer_Al]

After 5 years, have u understood it know?

[@KSATica]

So do these pair always like come together or are meant to be like destiny if you find your soul mate. (A) always matches with (T) as well as (C) matches with (G). Question isn't there like a special case or one of the those rules that the letter (C) has to be paired up with (A) but what about (T). C=A/T I don't know ;) just guessing and I took a stab at it and I'm pretty sure it doesn't make sense but since I'm coming off a math mind since all I learned in my classes in OSU is manipulation.

[@joedart8449]

I'm not sure if I understand this question, but if you are asking could C be paired up with A, the answer is no. Get a basic description of DNA (search youtube for "central dogma DNA") and it will tell you why in DNA, A normally attaches to a T and why C normally attaches to a G from the other strand.

[@bm7u]

He wrote the whole board.Maybe that's what I'll have to expect when I study genetic engineering.

[@zhengchaobong2112]

Subscribed =)

[@kundhan829]

oooooooo it protect bacteria from viruses....i am impressed

[@anilkumarsharma1205]

found that genome which gives oils in plants so we got directly patrol from trees and oil plants genome mixing

[@arijitpalit2756]

Are you working on biofuel? I would like to know more on that.

[@papaisduniyakapapa]

@@arijitpalit2756 IDC, But Maybe They Used A Tree Called Jatropa For BioFuel, Isn't It?

[@humeeayisha3199]

❤️👍🏻

[@zhengxu1186]

molecular biology is boring. But This class is really interesting. well done

[@muddypalmsera]

I have questions, but do you answer comments?

[@mitocw]

MIT OpenCourseWare is intended as a publication of MIT course materials, not as an interactive experience with MIT faculty. MIT OpenCourseWare does not offer users the opportunity for direct contact with MIT faculty. You are welcome to post questions and see if someone on KZbin can help you.

[@farahali5754]

I got the mechanism of enzymes

[@mattf1787]

Where does 4 to the 6th come from?

[@dianamaeponce5847]

@Bablu Sahu amazing!

[@farahali5754]

I watched

[@anilkumarsharma1205]

gives number to the genome and we found that limit and structure and size and arrangement of any genome probability gives total number of combination and permutations and pauli exclusivity principles the number of elements repetition and we got the ultimate behaviour of genome mixing gives desired result😋😋😝😝😝

[@jerrypeukert5732]

It seems disturbing that you can buy such things as though they are a commodity. Is everything for sale, even the basics of life, it doesn't seem right.

[@KSATica]

What is exactly 5 prime. Can anyone lend me a hand :)

[@rustyshackleford9877]

Peruviantank Do you stilp need some help😛 If yes then let's discuss

[@LuKa-mf9qe]

so it turns out... 😄

[@shellstorm1]

Great for the mind of a bored 12 year old

[@rakeshsreeramkr1675]

3:07 my hearing ability lost RIP headphone users

[@greenbeecolony1911]

Prehistoric days lol 😆😅

[@kundhan829]

bingo

[@l.m.mccormick1470]

Isn't bacteria so smart! lol... God bless.

[@kyleboulanger7835]

Back to see Lander in the White House Cabinet

[@steez2510]

Wow my professors suck 😒😑

[@fluxpistol3608]

lol basic