Best Liquid Culture Inoculation Tek!! Fully Colonized Grain Jars in Record Time

[@KrYmZuNhEaD]

Hey man! Thanks for sharing this sick tek. Can I ask what type of needle that is? It looks great for pulling up lots of lc. (BTW, you seem like a super chill dude!) Thanks brother ✌️

[@russmhunt]

It's typically a 16g needle used

[@davids11131113]

What I do for continued propagation now is when my spawn is going to substrate I save some in a few ketchup cups, give them about a week to get real fuzzy then with a new needle inject a syringe of sterile water into it right thru the lid, tape over it and shake it up then draw it back into the syringe and inject it right into new grain I’m starting. They can stay in the fridge too for whenever I want to use them. Super simple and always works.

[@RickL555]

That is freaking amazing! Absolutely brilliant. I've been doing regular ol' G2G transfers but this definitely sounds like something worth trying out. Thanks a million.

[@asshoub]

great idea , ive been using around 2 mls dropped on the sides onf the jars 4 diff places. when mycelium starts to grow it forms lines from the top down and a ring at the bottom of the jars, looks cool, and fast, i will try your method and see how it goes

[@RickL555]

Ok, please do. Thanks for dropping by.

[@RickL555]

Thanks man, I really appreciate it. I'm extremely fortunate that my sister works at a hospital and so she has access to the gear I use. You can repurpose the barrel from spore syringes that you order (or just order some the same size). The needle part is an18 gauge (try not to go any smaller that that if you can) and it's 3 1/2 inches long. Also needle sizes get smaller as the number goes up. 22 is way smaller than 18 as an example so make sure the one you get is in the 16-18 gauge range. I hope that helps. Good luck amigo! Thanks for dropping by.😃

[@KrYmZuNhEaD]

Thanks for replying man! Rad, I've sussed one of them out. Peace and mush love! ✌️🍄

[@RevShifty]

When you're using so much LC that you get pooling, especially in a medium like WBS, you can also add a tablespoon or two of BRF powder to the jars before you PC them. That will even out the moisture, keep things drier, and still provide a food source to the mycelium. To be honest, the times I played around with adding some BRF (also when I used WBS, so it has been some years), I swear it would colonize even faster. I think having tiny grains of food to much on coating the larger grains, each of those BRF grains now also hosting living mycelium, seems to make it even quicker.

[@RickL555]

Awesome thanks. Good to know I will definitely keep that in mind. Happy growing!

[@Bhakta_Mark]

You can plant bird seed but you won't grow birds

[@RickL555]

Ha! That's a good one

[@mr.ganja42O]

In my experience added in a extra 2 cc or 3cc increase my colonize chance by alot. The growth is so much faster when I add in say 5cc of liquid culture then say 2ml into my grain jars. Make sure the jars stay in a warm area of the house and those jars will be done within 2-3weeks on average. Depending on what size jars you use you could get full growth within a week if done correctly. Cheers and mush love y'all. Happy cultivating!

[@RickL555]

Right on, thank you!

[@apdwilly4632]

Hey man, are you using a full 10cc per jar? Could u test the speed time of using maybe 2cc vs 10cc with the method? Thanks cool vid

[@RickL555]

Yeah, fully loaded syringe and thank you! That's a really good idea for a follow up vid.

[@roycethomas]

good music selection, what artist?

[@RickL555]

Hi , thank you. It was some royalty free tunes I found on Adobe Stock. Unfortunately they didn't list the artist. I've always wondered that myself though. Have a good one.

[@Kevfactor]

so just drown it in home made LC. I've been having a lot of trouble at the colonization part. I had some popcorn a while back colonize but everything since has been a dud. Switched to rye and WBS with no luck. THink I'll try making LC out of syringe so I'm not scared to use it hehe. :)

[@RickL555]

Right on amigo . Good luck and happy growing!

[@RickL555]

Are you drying your grains before sterilizing them. The can stall and get slime molds if they're too wet. Also over shaking or shaking them too early (if you're doing the 30%) can cause stalls. That's why I love with this tek. Least amount of steps and

[@Kevfactor]

Ya drying em.i did the paper towel thing but i wonder if it's too dry. Some of the no soak no simmer recipes seem to look wetter than when i put in the jars. Also ya i mess with it too much with the shaking. Yikes it going to be tough for me to leave it alone lol. I also put them in a box with a pad on the side. I wonder if that causes CO2 and stuff@@RickL555

[@RickL555]

Too dry takes a little longer but is way better than too wet. Check out my WBS vid to learn how to dry every batch consistently. I learned a lot of patience from home mycology but used to obsess over it when I was a beginner. @@Kevfactor

[@RickL555]

Haha no shit. I used to run to my closet with a flashlight to check the mycelium every half hour. Now I just let it do it's thing.

[@thelastrebelshow1627]

I’m surprised the hot needle doesn’t kill the mycelium as it passes through. So you can go straight from flame to jar port with a hot needle?

[@RickL555]

Yup, the first tiny little squirt cools it down just fine. The redder the better. Just made that one up 😁

[@thelastrebelshow1627]

@@RickL555 That’s what I told her. 😂

[@RickL555]

@@thelastrebelshow1627 hahaha

[@plamenconev3387]

Hi, I have two questions: Do you shake the jars during colonization? One more question, do you think coconut fiber is a good food source for the fungi!? Thanks in advance ❤️

[@RickL555]

No shaking. It's an extra step that I started skipping a couple of years ago. It can increase the chances of your jar stalling out and sometimes the mycelium gets weakened a little and grows slower. Maybe if I were still using spore syringes I might but a good, healthy LC grows so fast the shake step was not needed. Thanks for dropping by!😃

[@RickL555]

Yup, coco is the best un less you're growing a wood or a dung lover look up the Philly Teacher CVG vid. That's what got me started. Happy growing amigo!!

[@plamenconev3387]

@@RickL555 hi mate, before I read your reply I had already shaken the jars! There is 30 percent colonization, I noticed that there was a small area left at the bottom that was stuck and did not want to crumble. I also feel that the beans look a little dry! The temperature is 27-30 degrees Celsius. I have a feeling that I'm doing something wrong, I'm asking for help and advice.

[@RickL555]

You're probably fine but keep an eye out for contamination. Give it a few days. If there's no visible mold and if it's not all slimly you should be good to go. Just don't re-shake them another time. It's hard when you a beginner because it could be a couple of weeks before you'll know if you screwed up or not. It can be stressful at times. Check out the Shroomery message boards. Lots of good info there too. Good luck!!@@plamenconev3387

[@plamenconev3387]

@@RickL555 Is 27-30 degrees Celsius good for colonization? Maybe that will dry out the grain!

[@chascodelviso]

Did I miss something or did you mention your temperatures??

[@RickL555]

Anything higher than 82 degrees can make it a better environment to contamination to grow . I'm not sure if it's true but I also read that it can be a couple degrees warmer inside the jar than the incubation area itself so I keep it around 78 to be safe. It's been working fine. Happy growing amigo!!😀

[@oh.no.noodles]

This is a great video, but I think the name is a bit misleading. It's more about how to inoculate with LC and not how to prepare LC. Perhaps make a video explaining your recipe and how you inoculate your LC. Do you work with agar?

[@RickL555]

That's a good idea. I'm wrapping up an editing project this weekend and then next week I'll get started on an LC vid. Yes, I'm working with agar. Took me a while but I got in the habit of testing all my LC on agar before putting it to grains.

[@londonfleet1099]

I have a jar of LC as well, and was wondering what can I do instead of using agar to test the sterility. How are you knowing you won’t contaminate your grain? I don’t have agar and I would rather do it this way without contamination.

[@RickL555]

Agar is the only way to really know for sure. Also learning how to spot the difference between healthy mycelium and mycelium that is bacterially stressed, goes a really long way. If it's marshmallow creamy texture, jars are sweatier than normal, seeds against the jar glass don't fully cover and little patches of spikes.. These are the sings that your spawn has something funky in there. Might still grow mushies but they will be little mutants and you won't get as many flushes. Good luck amigo!

[@aceagain7483]

Inoculate a smaller batch of grain...if it's healthy, Grain 2 grain, if not,toss it

[@andrewmodell5889]

So stamets said if you freeze the shrooms on water then let it thaw it pulls out oll of the juice ..said it really works can you try it ...

[@RickL555]

Hi, I think you're referring to Stamet's Blue Juice Tek. I haven't tried it yet but it's been on my list for a while now and thanks for reminding me. I do have some freshies to experiment with so maybe I'll do that this weekend. I'll drop you a message and let you know how it goes. Thank you

[@asshoub]

HOW DID IT GO@@RickL555

[@RickL555]

Hey, buddy, I never got around to trying it. Took more fresh mushrooms that I had at the time but I'm hoping to give it a go sometime this summer.@@asshoub

[@Houseofmycology]

I have ape colonizing popcorn and it took 3 days to begin seeing mycelium growth! What species is that?

[@RickL555]

Oh man, that was a while ago so I completely forgot. Happy growing!!

[@Houseofmycology]

@@RickL555 oh ok it’s cool ty

[@MattRoadhouse]

APE is a slow goer mutant, but that adds to its potency I believe

[@RickL555]

Yup, agreed. 🙂 @@MattRoadhouse

[@asshoub]

OW LONG USUALLY APE TAKE TO COLONIZE AND FRUIT. @@RickL555

[@ZenAndPsychedelicHealingCenter]

Good video but it's best to put the LC to agar first. There is also no need to throw out sunflower seeds and floaters from WBS. The Shroomery has more up to date tek on this that can help and makes it clear why sunflower seeds are just fine, among other things.

[@RickL555]

I have a bunch of chubby squirrels that would disagree with that. They think I'm throwing out the perfect amount of sunflower seeds.

[@RickL555]

Love the Shroomery, I'll look into it, Thanks buddy !

[@mattyb3490]

WBS is a very good sub for your LC / Agar to grow! I’ve been using it for monotubs with amazing results!

[@Enochulate88]

Bells on

[@Enochulate88]

I just realized I should stop breaking jars up with my hand they could be weak and shatter into my ✋

[@badmandansanchez1823]

Rolled up towel and don't be a hero

[@iCeDoG420]

Hey Man. New grower here. I just want to know if it is normal for the mycelium to be a light light green colour amd not pure white

[@RickL555]

No, that doesn't sound good.

[@RickL555]

Just dawned on me that pure white is also a sign of contamination. Like Trich before it turns green is noticeably several shades brighter than mycelium. And bacterial growth can have these smudges that sometimes grow little spikes and it's also noticeably a brighter shade of white. Anything that looks marshmallow like is also a bad sign. It's all a major pain in the ass but after a while you get the eye for this kind of stuff. Good luck Amigo!!

[@godseek444r]

How long did it take you to figure out that all that shit turning blue was mold Oh my gosh

[@RickL555]

You know it took me a sec to realize it but I got your question mixed up with someone else. Ha, you must have thought I was having a stroke. Slime mold doesn't turn blue that I'm aware of. Yeah, I didn't believe this would work at first either but when you shake the jars a few times that fully loaded rig gets spread around and you can hardly tell it was ever there. I was having problems with the WBS I was getting and thought I could fix the issue by simmering it for much longer and than I normally would and that's when the slime molds issue started. Way too much moisture. Now that I've switched to using popcorn kernels exclusively I'm also back to loading my jars with as much LC as one syringe will hold and so far it's working out just fine.

[@joshuajensen5868]

What portions are put in jar first?

[@RickL555]

I fill the jar with birdseed to around the 600ml line.

[@chaseTheCase9]

8 days!? 😯 At 25 days I still barely have any white showing 😳

[@RickL555]

That's a long time. Sounds like maybe you grains are too dry. That can make colonization drag on forever. Good luck

[@chaseTheCase9]

@@RickL555 makes sense thank you for that information 👊🏼 I’m gonna let them keep going I have time and I’m gonna make some more jars soon so I’m not stressing.

[@RickL555]

@@chaseTheCase9 Yup, my WBS vid has a cool trick for getting all your grains the same consistency. Been working great for years now. Good luck amigo! Happy growing

[@chaseTheCase9]

@@RickL555 thanks bro! I appreciate you!

[@mahmutvural8885]

How many cc liquid culture do you use for 100 ml bird food?

[@RickL555]

Not much for an amount that small. No more than 3 cc. Too much LC can lead to slime molds.

[@mahmutvural8885]

@@RickL555 according to your logic, you put 30cc liquid culture in 1000cc bird food, is it correct?

[@RickL555]

@@mahmutvural8885 No, I guess I meant that I just would risk putting any more LC than that (3cc 's) into an amount that small. 100ml of bird seed isn't much at all. What I've been doing these days is using 6cc's of LC into a jar of bird seed filled to around the 600-700 ml line. I could probably use more than that but then I risk having slime mold issues so I play it safe and just use 6cc. It's all relative to how long you soak and simmer for and what type of bird seed you're using so plan on a bit of trial and error to adjust for these variables.

[@badmandansanchez1823]

@@RickL555 thanks for that explanation. Slime mold sucks

[@RickL555]

​ Yes, indeed. I was having some issues with the WBS I was getting and thought I could solve the problem by simmering my birdseed for much longer than I normally would. That put a lot of extra moisture in the jar and that's when I dropped the LC to 6 units per jar to avoid the slime molds. Before that I had been using a full syringe of LC with amazing results!! You have to shake it so LC can't pool up in the jar and you're all good. WBS is cheap and it had been very reliable until the day it wasn't anymore. It's not meant for human consumption so it's processed, packaged, stored and shipped much differently than if you got those same raw grains from the hippie food aisle. Anything from rat turds to molds and bacteria can hitch a ride in WBS at any point along the way and some of that stuff can survive the pressure cooker. I found a 50lb bag of popcorn from a restaurant supply store for about $35. I'm back to loading my rig with as much gooey goodness as one syringe will hold. Good luck Amigo!

[@mahmutvural8885]

how many grams of bird food in the jar

[@RickL555]

I've never weighed it so I'm not 100% sure. If your jar has measuring units on it then I would say around the 700ml line is good.

[@Dundeemac799]

Not really a LC tek is it.... This was more of a "how to innoculate jars" vid. Was hoping to see a vid showing and actual LC preparation...

[@RickL555]

Crap, yeah I see what you mean. I'll take that into consideration for future titles. Thank you

[@Cloner442Central]

What kind of bird food

[@RickL555]

I was getting the big bags of Wild Bird Seed at Lowes because that brand had the least amount of Sunflower seeds in it but stopped using it after I started having issues with bacteria that survived the pressure cooker. I now use popcorn kernels meant for human consumption. You can find a 50 lb bag for about $35 if you shop around.

[@Enochulate88]

First

[@Enochulate88]

Sabs are cool and work n stuff but I must be retarded because they are hard for me to use. Flow hoods is all I want. Where did Yu get such a long needle

[@Enochulate88]

And why do your grains look black or like coco

[@RickL555]

My sister works at a hospital so I get hooked up with needles but you can get them online. Don't go smaller that 17 ga. The length is 3.5 inches.

[@RickL555]

I got a 24x24 flow hood a couple months after I filmed this vid.

[@Enochulate88]

Do you like bella Bora

[@RickL555]

@@Enochulate88 I never tried one out. Looks pretty slick though. I was able to find a flow hood for about $1000 on Esty.

[@andyfulton2316]

I would never shake a jar that was just inoculated, unless u love waiting twice as long for colonization. I've done it. It was awful.

[@tangyonions]

He used liquid culture, when using spores the spores need to grow into hyphae and then combine to form mycelium In a liquid culture the hyphae are already clumped together and so shaking it spreads the amount of inoculation points I might be wrong tho idk I never grew mushrooms but that's my understanding anyway