How Much LC to Use? My Ideal Ratio of Liquid Mycelium Culture to Grain for Mushroom Spawn.

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Over the last few years, I've received a lot of questions about how much liquid culture is needed to inoculate grain. In this video, I dive in to the factors at play and share my ideal ratio of liquid mycelium culture to grain in order to have the greatest chance of successful spawn generation.

[@NicoleCtirad]

Mushrooms did wonders in my life. I suffered severe trauma several years ago after divorce with my husband. Got diagnosed with cptsd. Spent my whole life fighting cptsd. I suffered severe depression and mental disorder. Not until my son recommended me to psilocybin mushrooms treatment. Psilocybin treatment saved my life honestly. 6 years totally clean. Never thought I would be saying this about mushrooms.

[@Ronkaja]

Congrats on your recovery. Most persons never realizes psilocybin can be used as a miracle medication to save lives. Years back i wrote an entire essay about psychedelics. they saved you from death bud, lets be honest here.

[@DonnHowes]

YES sure of Dr.benfungi. Did straight shrooms in few days. Left me like a blank slate after words, no more addictions, pains, ptsd and depression. Shit saved my life, all thanks to Dr.benfungi

[@Mcdogmom288]

100% agree I used to have Psychosis and paranoid thoughts like "people thinking about me talking about me etc. Very odd behavior after getting off Adderall from 7-16. Antidepressants at 18-29. 31 now. I took way to much, but took about 20g of Gold caps (Psilocybin containing mushroom) I analyzed my entire life. The emotions that came out helped me understand behavior etc more. Wont ever need to do it again because I'm happy and contempt forever, but I wish more people did this to alter their perception of reality. Would help with healing much trauma

[@VanRyan-bv7du]

How do I reach out to him? Is he on insta

[@Iiisslogan-co6np]

wish they were readily available in my place. Microdosing was my next plan of care for my husband. He is 59 & has so many mental health issues plus probable CTE & a TBI that left him in a coma 8 days. It's too late now I had to get a TPO as he's 6'6 300+ pound homicidal maniac. He's constantly talking about killing someone. He's violent. Anyone reading this Familiar w/ BPD know if it is common for an obsession with violence.

[@shaggygoatboy1125]

3:50 To get to the point.

[@Waterloo_Bear]

😂😂😂

[@ScottWConvid19]

There's no such thing as a stupid question. There are, however, stupid people and stupid people don't usually ask questions. But not all questions have a simple answer, yet you took this seemingly complicated process and simplified it for us. Thank you

[@johnbauby6612]

I always appreciate and enjoy your vids even if the info is not new to me. Thanks for taking the time to make and post.

[@vtphoto1]

Thank you for your instruction and supplies!

[@raisnaix]

Great video brother. Your channel is coming along great.

[@TheTaylorsVideos]

Definitely will be using your stuff soon, thanks

[@N9524Q]

I do about 2 cc per pound of hydrated grain of wheat berry or brown rice or popcorn. So your recommendation seems to be on the high side. Usually fully colonized with one shake in 10 days to two weeks depending on the mushrooms. I usually stick to 2 different varieties of oyster and one of lions mane. Lions mane is definitely slower and may take up to 18 days. My conditions are at about 80° Fahrenheit. But I agree that it is a race with the mycelium usually winning

[@ixxgxx]

Yeah I thought on high side too. So north spore 3lb bags about 6cc ?

[@N9524Q]

@@ixxgxx I make grain spawn most of the time in 1 qt mason jars. With 2 cc I've liquid culture. I use about 1/2 pound fully colonized grain spawn to 5 pounds of fully sterilized substrate

[@Barbie.Boo1961]

@@ixxgxxI just bought from pnw , I have 2 grain bags going, just starting to grown , it’s been about 13 days for one and 7 for the other, DO I NEED TO BREAK UP THE GRAIN? ITS AND ALL IN ONE BAG? I THOUGHT I WAS SUPPOSED TO JUST LET IT Colonize then break it up and mix the dirt through it…. Dang I with I could sit down with someone, bad dyslexia here. Lol, seriously though my dyslexia can be brutal.

[@travisherbermann7249]

Really informative. Keep up the good work. I am very interested in learning about creating from dehydrated material.

[@AllThatIKnowNow]

I mistakenly added 10cc of liquid culture to 500g (about 1 lb) of grain, and now it's like this snowy, large, white dimorphous shape. I had previously removed it from the substrate and grain, all in one grow bag and put it in a shoebox. Then I had to remove the lid of the shoebox tub because the white blob was up against it.

[@u8myufo]

@OneEarth Mushrooms, informative video thanks. So given that a 10ml syringe would just about do a 5lb bag of substrate, could you not create say 500ml of homemade mycelium liquid culture from that one syringe of culture? Similar to that which a baker always has a culture of yeast in storage.

[@JohnSmoke-ih1ow]

Thank you

[@michaelgillespie1206]

You don't have to use very much of the LC solution, the key is to shake your jars every two days, or your bags whatever the case may be, your grain spawn should be colonized within two weeks, ready for the next step, growing out on substrate.

[@archieh4058]

IMO this is a misnomer, 2 weeks for what varietal of mushroom? Since we may or may not be growing the same thing, and more LC usually means a slightly faster cultivation period, i always say depending on your variety- it will take between 2 and 5 weeks for mycelium colonization

[@mr.ganja42O]

I put in 20ml of B+ liquid cultrue in one grain bag(about 2pounds of grain) ( LC is over 7months old and been store in a wardrobe) and get strong mycelium growth within 72 hours of inoculation. So do as much you want :) you can literally use half of a ML and still get great success!

[@thelastrebelshow1627]

What’s the best temperature to store inoculated grain jars at? I’m thinking 75 does that sound right?

[@leeirwin6239]

I've put in about 5-7 cc into around a half full jam jar worth of millet.......so stressed I think my grain is too wet too. I'm on day 4.....do i shake the grains up every day? And my temperature checker/ hydration checked has a in and out setting the in setting says its hotter then in is....who do i do to check the temperature...lol? The in or out...thanks guys. So glad i found this channel....x

[@dany-922]

I just thought of this, KZbind it, and found this! Cheers man! I have Pink & Golden Oyster LC almost ready, so I just ordered a refill kit ;)

[@xxg0t3mxx]

I use 1cc per lb does the trick just got into agar though so hope it works out

[@HaydenVanGundy]

What if you bring your grain below field capacity then added extra lc to adjust the hydration to proper field capacity

[@judyhowell7075]

My go to guy

[@Animalis_Mundana]

Is there supposed to be a difference before and after P.C? Seems like it has settled a bit, 20 hr soak, 12 minute simmer, strain cool down until passes spoon test, fill jars, foil etc, pressure cook for 90 at 14-15 PSI. Idk, just seems like less in jar after PC but they appear to be hydrated. No pooling water, plenty of condensation after inside after, I let it cool naturally in PC for about an hour, about 30 after depressurized, i tighten jars right away and shake.let sit overnight.

[@imohpius8956]

Hello bro how are you doing, plz I need your advice. My sterilizing jar broke while I was sterilizing my LC.

[@andyfromtoystory7426]

30cc of LC is absolutely insane for 5lbs, my 5ccs do just fine 😂

[@jasonvicente3712]

I agree 😂😅 a 12cc syringe I’ve inoculated 2 5lbs bags

[@6hottk]

Seems like a rude way to state your findings.

[@ricksanchez3204]

Go make a channel then show us prove it instead of talking shit

[@ricksanchez3204]

Also this guy "doubles up" his LC so to speak, do you think that may have something to do with why he uses a lot? That's a sincere question not a put-down tho... Did you even consider that before saying it was insane or did you just see somebody doing something different than you do it and decided that's a good enough reason to try to insult him?

[@BLACK_CAT_TRAVEL_AGENCY]

​@@phillypharmerdepends on how fast you want it to spread

[@NetsarimTheWatchman]

I do 10ml LC for 1 gallon jars

[@ZeStaticWolf]

I feel like this is over kill you can do masters mix with 1cc with high chances of success. Even if it’s not fast, it’s prevents wet rot if you use that much.

[@Barbie.Boo1961]

Hi, just started learning to grow mushies. I really need help. I don’t understand why they have to be in a sterile environment and sterilize air? Love your stuffies

[@fisrtnamelastname3083]

Basically there is stuff In the air and environment that can and will try to grow in your medium. Sterilizing decreases the risk of things you don't want growing to grow

[@ZeStaticWolf]

There ate spores from mold and bacteria in the air from movement, breathing, and other mold sources. That’s why some people buy respirators and goggles and Lysol everything in a 5 ft radius. When you innoculate anything you have to realize it’s the same perfect environment for molds and bacteria’s to grow so your trying to give the mushrooms a head start by creating a sterile environment with only them having a success rate!

[@judyhowell7075]

Would you shake if adding spores?

[@TheGreatLake1998]

Yeah, disperse your inoculate for more rapid colonization. You can repeat this process after the mycelium has spread through some of the bag. Again, dispersing for a faster track to full colonization.

[@michaelgillespie1206]

If starting from spores, it's best to start them on agar, then isolate a vigorous strain of dikaryotic mycelium and use that to spawn your grain.

[@judyhowell7075]

@@michaelgillespie1206 I understand but I’m leaving town for a week and will attempt that next!Thank you

[@slimreaper7779]

Don’t use spores unless your using agar yr begging for contam

[@Mattysmeatsandeats]

If you get a liquid culture from a reputable seller can you use the same method to make more liquid culture?

[@bigtime4794]

That's a good question I'm assuming in way easier than spores. I just LC 'd with 10 drops but I'm confident it will work. I'm not in any hurry

[@dandy8308]

Yes

[@sabahhassan3140]

I watch all your vedio and try to understand but I can't I need Arabic translatin all times I failed and failed and failed to grow mushroom thanks

[@daphnecranes3009]

If you can't grow them, why just buy some to save you the stress order for them it will be delivered to you.

[@roy9161]

Does anyone know where I can Buy Spores please... This is for medicinal use for me.. can anyone help me please??

[@jhood8367]

I heard about a place called legends mycology thats got some lc syringes (they're called isolated syringes) great prices and pretty fast shipping. It's a way easier and faster start w a lc as opposed to spores.

[@PPP-on3vl]

THE MORE THE BETTER

[@n8n888]

I feel like that's tooooo much lc.

[@lordhaitian3853]

Bro side note: You look like Jason Statham!!!!!!!!!!!!!!!!!!!

[@610231718]

I thought it was 448grams in a pound not 454. Math time, 28grams(in an ounce) x 16 ounces = 448g. if anyone can explain why he said 454 that'd be rad to unless it was a genuine mistake

[@bmartin2304]

There is actually 28.35 grams in an oz. we been getting ripped off all these years!

[@SachaLotus]

@@bmartin2304😂😂😂😂

[@crapisnice]

hi, another freeloader here, i have never made this but i think the volume of liquid culture should be taylored by the growth speed rate of different mushrooms and the time we want to wait. thanks a lot for this exploitative patron content when should be a team group of broadcasting instead youtube jesus

[@bamba2046]

And no one asked why don’t you have a mic? Just saying.

[@journeytoascension7018]

448 grams per pound. BASIC AZZ MATH.

[@GlorifiedPizza]

453.59 grams in a pound.

[@420ramborip]

close but not quite a lb.....

[@610231718]

these people in your comments need to show us there math to get there numbers lol show your work

[@fattymcbastard6536]

I wonder if you can get away with a higher inoculation rate on grain prepared by the no soak/ no boil method. This is the method I use, and I wonder if the grain has the capacity to absorb a little bit more.

[@ZeStaticWolf]

This is what I was wondering as well since he doesn’t soak his rye before boiling and pressure cooking. He might be forcing the grain to absorb the lc by starving them of liquid if so that’s kinda genius imo.