9. Isoenzymes or Isozymes

Definition
different forms of the same enzyme catalyzing same reaction
How they are formed
1. Different genes- true isozymes
2. all variants are present in same individual
3. isoforms- due to post translational modification
4. allozymes- from different alleles
characteristics
1. electrophoretic mobility
2. substrate specificity and Km
3. Heat stability
4. Inhibitor
5. Coenzymes
6. Antibody sensitivity
7. Tissue of origin
examples
LDH- 1 to 5
CK 1 to 3
ALP-6
clinical significance

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Bhai isoenzyme satyanarayan me kis page pe hai?

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[@pmuralidharan5042]

Sir you have said that alpha 2 HL is present in liver and is sensitive to heat but in my textbook it is given as another isoenzyme of ALP that is present in bone is also heat labile. Can two of isoenzymes be heat labile sir ?

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I didn't understand heat labile property of isoenzyme ??

[@aasemahsan]

11:32 LDH 13:59 CK (Creatine Kinase) 15:57 ALP (Alkaline Phosphatase)

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Sir please make lacture classification of isoenzyme and significance of isoenzyme 🙏

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Excellent

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Please suggest any good book for enzymology in english. Thanks

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Vasudevan for MBBS

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Sir. Normal range OF ALP is 30 to 300 or 30 to 120 IU/L???

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👏🏻

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CK-M peak is also differnt

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What is the 6th isoenzyme of alkaline phosphatase ??

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Leukocyte alkaline phosphatase

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Sir H3m and H2m2 ..where they present

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