BioCoupler Best Practices

[@niroshajayskody333]

When you use biocuplar for philodendron multiplication what is the most suitable amount of the following chemicals 1.BAP 2. Half or full ms 3.sugar And how many times do we need to turn the biocupler per week? ,

[@jst3455]

What would happen if I tried TC with a really large explant (like a 6 inch long piece of new, actively growing plant growth on a tropical rainforest cactus)? Do you think this could potentially be used to accelerate plant growth and maturity, if I also never divide it? Also I bought a biocoupler and can't wait to experiment with it!

[@PlantCellTechnology]

6 inches (that is about 15 cm) of the explant is too large. You don't need to use that big explant for the tissue culture procedure. IF you want to use a larger explant then try 2-4 cm. that will work.

[@jedz86]

hi did u autoclaved this sheet of paper in beginning of the film?

[@PlantCellTechnology]

No. you never autoclave a sheet of paper. We just keep the new box of tissue after opening its packet in the TC lab itself to wipe the culture area.

[@craigheath3800]

Where do you purchase the Micro Spore filter for replacement for the Bio-coupler at? also what type

[@NATOF-35]

When are you going to do a full cannabis media break down? :)

[@PlantCellTechnology]

Thanks for showing your interest in our videos. We're trying to create content on diverse topics and many are lined up to be released in coming weeks. But, we will try our best to create a series on cannabis soon. However, before that, if you need immediate assistance with your cannabis tissue culture, you can choose PCT's cannabis consulting service to get your answers instantly.

[@amtd3808]

What happened to seed treated with hydrogen peroxide ... Are the results r positive ???

[@PlantCellTechnology]

In the same video, you will find that seeds have been transferred to tissue culture and we have obtained tiny shoots growing in each of them. :)

[@samarthshukl7463]

What is this in the jaar water or media??

[@PlantCellTechnology]

It's media in the jar.

[@niroshajayskody333]

What is the difference between the media used in bio couppler from normal semi solid media( full ms or half MS)?

[@PlantCellTechnology]

Full MS and Half MS are different and Semi-solid and liquid media is different. Full MS and Half MS dictate the strength of the media. half MS would be the use of half the quantity of macro and micronutrients including sucrose for your tissue culture process. The biocoupler need liquid media (zero agar), while the semi-solid media is a media containing agar concentrations of 0.2 to 0.5%.

[@niroshajayskody333]

Thanks soo much ☺

[@ryanfenimore4114]

Does the vent filter make it so that the jars can be sealed in the autoclave?

[@agrippanyagwaya2308]

Were can I buy biocouplers in South Africa

[@PlantCellTechnology]

Hi there, Please find the distributor closest to your location on this page: www.plantcelltechnology.com/distributors/ Or you can order from our website directly: www.plantcelltechnology.com/pct-store/

[@RusticRaver]

Hi Francisco! Yesterday I was thinking after all these free lessons that I would order 6 biocouplers from you guys to thank you, unfortunately when I went to basket to pay, it turns out FEDEX defeats the purpose of sending things abroad, I'm in the UK here and 100£ of bio coupler turns into 350£ with postage, is there a way you could send stuff at a normal rate!? 30squids tops, thank you.

[@PlantCellTechnology]

Hey, Try contacting our distributor in the UK with your requirements. Here're the details: ASR Sciences Main Office Manchester, UK 08000 842 543 asrsciences.com

[@RusticRaver]

@@PlantCellTechnology excellent thx

[@Spencerrcr]

Would this be good for beginners? I'm still browsing through your videos and this caught my eye. I did want to ask, what is the liquid used? I was under the impression the liquid is usually thick due to the agar. Thanks!

[@PlantCellTechnology]

Hi, Of course, beginners can also use these. The media used is normal MS media supplemented with sugar and plant hormones. Do not use agar with Biocouplers, you don't need it.

[@agrippanyagwaya2308]

I recently received my MS media and it's written 5ltr on the side of the bottle,my question is , can I still add up the sugar when preparing the tissue culture media

[@PlantCellTechnology]

@@agrippanyagwaya2308 Yes, you will need to add that. check out some videos we have on our channels to best utilize the starter kit.

[@agrippanyagwaya2308]

@@PlantCellTechnology Wy agar is so expensive lke that

[@PlantCellTechnology]

@@agrippanyagwaya2308 Are you talking about all agar in general?

[@niroshajayskody333]

Btw thanks for making these amazing videos they are really helpful 😊

[@PlantCellTechnology]

Thanks, Nirosha!! If you are interested in tissue culture, check out our website www.plantcelltechnology.com for any of your tissue culture requirements.